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  1. iGABASnFR2: Improved genetically encoded protein sensors of GABA

    This article has 19 authors:
    1. Ilya Kolb
    2. Jeremy P Hasseman
    3. Akihiro Matsumoto
    4. Thomas P Jensen
    5. Olga Kopach
    6. Benjamin J Arthur
    7. Yan Zhang
    8. Arthur Tsang
    9. Daniel Reep
    10. Getahun Tsegaye
    11. Jihong Zheng
    12. Ronak H Patel
    13. Loren L Looger
    14. Jonathan S Marvin
    15. Wyatt L Korff
    16. Dmitri A Rusakov
    17. Keisuke Yonehara
    18. GENIE Project Team
    19. Glenn C Turner
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This manuscript reports the development and characterization of iGABASnFR2, a genetically encoded GABA sensor that demonstrates substantially improved performance compared to its predecessor, iGABASnFR1. The work is comprehensive and methodologically rigorous, combining high-throughput mutagenesis, functional screening, structural analysis, biophysical characterization, and in vivo validation. The significance of the findings is fundamental, and the supporting evidence is compelling. iGABASnFR2 represents a notable advance in GABA sensor engineering, enabling enhanced imaging of GABA transmission both in brain slices and in vivo, and constitutes a timely, technically robust addition to the molecular toolkit for neuroscience research.

    Reviewed by eLife, PREreview

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  2. Modality-Agnostic Decoding of Vision and Language from fMRI

    This article has 6 authors:
    1. Mitja Nikolaus
    2. Milad Mozafari
    3. Isabelle Berry
    4. Nicholas Asher
    5. Leila Reddy
    6. Rullen VanRullen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This manuscript introduces a potentially valuable large-scale fMRI dataset pairing vision and language, and employs rigorous decoding analyses to investigate how the brain represents visual, linguistic, and imagined content. The current manuscript blurs the line between a resource paper and a theoretical contribution, and the evidence for truly modality-agnostic representations remains incomplete at this stage. Clarifying the conceptual aims and strengthening both the dataset technicality and the quantitative analyses would improve the manuscript's significance for the fields of cognitive neuroscience and multimodal AI.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  3. A Forebrain Hub for Cautious Actions via the Midbrain

    This article has 4 authors:
    1. Ji Zhou
    2. Muhammad S Sajid
    3. Sebastian Hormigo
    4. Manuel A Castro-Alamancos
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This useful study uses fiber photometry, implantable lenses, and optogenetics to show that a subset of subthalamic nucleus neurons is active during movement, and that active but not passive avoidance depends in part on STN projections to substantia nigra. The strength of the evidence for these claims is solid, whereas evidence supporting the claims that STN is involved in cautious responding or the speed of avoidance is incomplete. This paper will be of interest to basic and applied behavioural neuroscientists working on avoidance if suitably streamlined to support the strongest claims.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  4. Pathogenic O-GlcNAc dyshomeostasis associated with cortical malformations and hyperactivity

    This article has 15 authors:
    1. Florence Authier
    2. Asad Jan
    3. Islam Faress
    4. Christian Stald Skoven
    5. Iria Esperon-Abril
    6. Shagana Tharmakulasingam Balasubramaniam
    7. Kévin-Sébastien Coquelin
    8. Jens R Nyengaard
    9. Carsten Scavenius
    10. Benedetta Attianese
    11. Oscar G Sevillano-Quispe
    12. Simon Fristed Eskildsen
    13. Jesper Skovhus Thomsen
    14. Brian Hansen
    15. Daan MF van Aalten
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This is an important study that takes a key step towards understanding developmental disorders linked to mutations in the O-GlcNAc transferase enzyme by generating a mouse model harboring the C921Y mutation. The study thoroughly examines behavioral and anatomical differences in these mice and finds behavioral hyperactivity and learning/memory deficits, as well as phenotypic differences in skull and brain formation. However, the experimental evidence is incomplete owing to discrepancy in OGT protein/RNA levels in the C921Y mutant mice in this paper and the previous paper ("Neurodevelopmental defects in a mouse model of O-GlcNAc transferase intellectual disability "). This line of research will benefit from investigation of the differences in associated glycoproteins and mechanistic insights. This study will be of interest to those studying neurodevelopment, learning and behavior, or associated brain mechanisms.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  5. Inferring variant-specific effective reproduction numbers from combined case and sequencing data

    This article has 2 authors:
    1. Marlin D Figgins
    2. Trevor Bedford
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study provides new important insights concerning pathogen variant-specific reproduction parameters from molecular sequencing and case finding. The methods for inferring which variants will likely emerge in subsequent epidemic cycles are solid. This article is of broad interest to infectious disease epidemiology researchers and mathematical modellers of the COVID-19 pandemic.

    Reviewed by eLife, ScreenIT

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  6. Mood computational mechanisms underlying increased risk behavior in adolescent suicidal patients

    This article has 9 authors:
    1. Zhihao Wang
    2. Tian Nan
    3. Fengmei Lu
    4. Yue Yu
    5. Xiao Cai
    6. Zongling He
    7. Yuejia Luo
    8. Ting Wang
    9. Bastien Blain
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study combined careful computational modeling, a large patient sample, and replication in an independent general population sample to provide a computational account of a difference in risk-taking between people who have attempted suicide and those who have not. It is proposed that this difference reflects a general change in the approach to risky (high-reward) options and a lower emotional response to certain rewards. Evidence for the specificity of the effect to suicide, however, is incomplete, which would require additional analyses.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  7. An increase of NPY1 expression leads to inhibitory phosphorylation of PIN-FORMED (PIN) proteins and suppression of pinoid (pid) null mutants

    This article has 6 authors:
    1. Michael Mudgett
    2. Zhouxin Shen
    3. Ruofan Kang
    4. Xinhua Dai
    5. Steve Briggs
    6. Yunde Zhao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study concerns a highly interesting and biologically relevant topic, the regulation of the PIN auxin transporter, which is of broad interest to the plant biology community. The authors propose NPY1 to act downstream of PID in auxin-mediated development by modulating PIN phosphorylation, which, if experimentally solidified, would expand our understanding of PIN regulation. While the genetic evidence is solid, the mechanistic role of NPY1 and the functional relevance of phosphorylated PIN residues are still uncertain. There are also concerns regarding experimental rigor and methodological transparency.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  8. Listening to the room: disrupting activity of dorsolateral prefrontal cortex impairs learning of room acoustics in human listeners

    This article has 6 authors:
    1. Heivet Hernández-Pérez
    2. Jason Mikiel-Hunter
    3. James Traer
    4. Jessica JM Monaghan
    5. Paul F Sowman
    6. David McAlpine
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment:

      This study addresses valuable questions about the neural mechanisms underlying statistical learning of room acoustics, combining robust behavioral measures with non-invasive brain stimulation. The behavioral findings are strong and extend previous work in psychoacoustics, but the TMS results are modest, with methodological limitations and over-interpretation that weaken the mechanistic conclusions. The strength of evidence is therefore incomplete, and a more cautious interpretation of the stimulation findings, alongside strengthened analyses, would improve the manuscript.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 1 listLatest version Latest activity
  9. Morphogenesis and morphometry of brain folding patterns across species

    This article has 7 authors:
    1. Sifan Yin
    2. Chunzi Liu
    3. Gary PT Choi
    4. Yeonsu Jung
    5. Katja Heuer
    6. Roberto Toro
    7. L Mahadevan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study presents a cross-species and cross-disciplinary analysis of cortical folding. The authors use a combination of physical gel models, computational simulations, and morphometric analysis, extending prior work in human brain development to macaques and ferrets. The findings support the hypothesis that mechanical forces driven by differential growth can account for major aspects of gyrification. The evidence presented, though limited in certain species-specific and parametric details, is overall strong and convincingly supports the central claims; the findings will be of broad interest in developmental neuroscience.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  10. Biophysical basis for brain folding and misfolding patterns in ferrets and humans

    This article has 7 authors:
    1. Gary PT Choi
    2. Chunzi Liu
    3. Sifan Yin
    4. Gabrielle Séjourné
    5. Richard S Smith
    6. Christopher A Walsh
    7. L Mahadevan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study characterises the morphogenesis of cortical folding in the ferret and human cerebral cortex using complementary physical and computational modelling. Notably, these approaches are applied to charting, in the ferret model, known abnormalities of cortical folding in humans. The study finds that variation in cortical thickness and expansion account for deviations in morphology, and supports these findings using cutting-edge approaches from both physical gel models and numerical simulations. The strength of evidence is convincing, and although it could benefit from more quantitative assessment, the study will be of broad interest to the field of developmental neuroscience.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  11. Bacteria detect neutrophils via a system that responds to hypochlorous acid and flow

    This article has 11 authors:
    1. Ilona P Foik
    2. Runhang Shu
    3. Serena Abbondante
    4. Summer J Kasallis
    5. Lauren A Urban
    6. Andy P Huang
    7. Leora Duong
    8. Michaela E Marshall
    9. Eric Pearlman
    10. Timothy L Downing
    11. Albert Siryaporn
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This is an important study reporting a new phenotype for a gene cluster that has previously been associated with the responses of the Gram-negative opportunistic pathogen Pseudomonas aeruginosa to flow fluid. Expression of the froABCD gene cluster is induced by HOCl in vitro and by activated immune cells, which produce these types of reactive chlorine species. Overall, the evidence presented by the authors is solid; however, the mechanism of fro-induction by HOCl remains unclear, and the evidence in support of the authors' claims is descriptive, which needs to be improved. This study is of interest to infection biologists interested in mechanisms of bacterial pathogenicity.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  12. An in vitro human vessel model to study Neisseria meningitidis colonization and vascular damages

    This article has 13 authors:
    1. Léa Pinon
    2. Mélanie Chabaud
    3. Pierre Nivoit
    4. Jérôme Wong-Ng
    5. Tri Tho Nguyen
    6. Vanessa Paul
    7. Charlotte Bouquerel
    8. Sylvie Goussard
    9. Pauline Smilovici
    10. Emmanuel Frachon
    11. Dorian Obino
    12. Samy Gobaa
    13. Guillaume Duménil
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this important study, the authors develop a microfluidic "Vessel-on-Chip" model to study Neisseria meningitidis interactions in an in vitro vascular system. Compelling evidence demonstrates that endothelial cell-lined channels can be colonized by N. meningitidis, triggering neutrophil recruitment with advantages over complex surgical xenograft models. This system offers potential for follow-on studies of N. meningitidis pathogenesis, though it lacks the cellular complexity of true vasculature including smooth muscle cells and pericytes.

      [Editors' note: this paper was reviewed by Review Commons.]

    Reviewed by eLife, Review Commons

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity
  13. Lipid Peroxidation and Type I Interferon Coupling Fuels Pathogenic Macrophage Activation Causing Tuberculosis Susceptibility

    This article has 19 authors:
    1. Shivraj M Yabaji
    2. Vadim Zhernovkov
    3. Prasanna Babu Araveti
    4. Suruchi Lata
    5. Oleksii S Rukhlenko
    6. Salam Al Abdullatif
    7. Arthur Vanvalkenburg
    8. Yuriy Alekseev
    9. Qicheng Ma
    10. Gargi Dayama
    11. Nelson C Lau
    12. W Evan Johnson
    13. William R Bishai
    14. Nicholas A Crossland
    15. Joshua D Campbell
    16. Boris N Kholodenko
    17. Alexander A Gimelbrant
    18. Lester Kobzik
    19. Igor Kramnik
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      Yabaji et al. reports a fundamental study highlighting the mechanistic connection for susceptibility to TB infection via the sst1 locus, this was shown to involve increased IFN and Myc production causing the down-regulation of anti-oxidant defence genes and chronic lipidation. Ultimately, lipid peroxidation may underlie infectivity and macrophage dysfunction. Overall, the data presented are compelling, supported by a well designed multi-omics approach and the findings will be of broad interest to researchers investigating the molecular mechanisms of TB infection.

      [Editors' note: this paper was reviewed by Review Commons.]

    Reviewed by eLife, Review Commons

    This article has 7 evaluationsAppears in 2 listsLatest version Latest activity
  14. Stimulus-Dependent Theta Rhythmic Activity in Primate V1 Predicts Visual Detection

    This article has 6 authors:
    1. Prasakti Tenri Fanyiwi
    2. Beshoy Agayby
    3. Ricardo Kienitz
    4. Marcus Haag
    5. Jaime Cadena-Valencia
    6. Michael C Schmid
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This useful study shows that stimuli of a certain size elicit theta oscillations in V1 neurons both in spikes and local field potentials, and monkeys performing a dot detection task on these stimuli show theta rhythmicity in their response times. This replicates previous findings showing rhythmic theta activity in V4 and behaviour when stimuli are presented in the receptive field along with a surrounding flanker stimulus. However, there is incomplete evidence that rhythmicity in neural activity is related to the rhythmicity in behavior, and the mechanisms underlying these oscillations remain unclear.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  15. Differential interfacial tension between oncogenic and wild-type populations forms the mechanical basis of tissue-specific oncogenesis in epithelia

    This article has 9 authors:
    1. Amrapali Datta
    2. Phanindra Dewan
    3. Aswin Anto
    4. Tanya Chhabra
    5. Tanishq Tejaswi
    6. Sindhu Muthukrishnan
    7. Akshar Rao
    8. Sumantra Sarkar
    9. Medhavi Vishwakarma
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study reports that an oncogenic population in an epithelium can either be repressed or spread, depending on the tissues. This is explained based on the differential interfacial tension hypothesis, and supported by pharmacological perturbations and numerical simulations using the vertex model. The study conveys a key message, but, as it stands, the strength of evidence is incomplete, and a more detailed analysis of the mechanistic origin of the different tensions and better comparison between experiments and simulations would strongly strengthen the message.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  16. Physiological febrile heat stress increases cytoadhesion through increased protein trafficking of Plasmodium falciparum surface proteins into the red blood cell

    This article has 7 authors:
    1. David Jones
    2. Hugo Belda
    3. Malgorzata Broncel
    4. Gwendolin Fuchs
    5. David Anaguano
    6. Stephanie D Nofal
    7. Moritz Treeck
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study provides compelling evidence that fever-like temperatures enhance the export of Plasmodium falciparum transmembrane proteins, including the cytoadherence protein PfEMP1 and the nutrient channel PSAC, to the red blood cell surface, thereby increasing cytoadhesion. Using rigorous and well-controlled experiments, the authors convincingly demonstrate that this effect results from accelerated protein trafficking rather than changes in protein production or parasite development. These findings significantly advance our understanding of parasite virulence mechanisms and offer insights into how febrile episodes may exacerbate malaria severity.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  17. Neural signatures of motor memories emerge in neural network models

    This article has 3 authors:
    1. Joanna C Chang
    2. Claudia Clopath
    3. Juan A Gallego
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents valuable computational findings on the neural basis of learning new motor memories without interfering with previously learned behaviours using recurrent neural networks. The evidence supporting the claims of the authors is solid, but it would benefit from stronger and clearer links with experimental findings. This work will be of interest to computational and experimental neuroscientists working in motor learning.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  18. Neisseria gonorrhoeae LIN codes: a Robust, Multi-Resolution Lineage Nomenclature

    This article has 6 authors:
    1. Anastasia Unitt
    2. Made Krisna
    3. Kasia M Parfitt
    4. Keith A Jolley
    5. Martin CJ Maiden
    6. Odile B Harrison
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study introduces the Life Identification Number (LIN) coding system as a powerful and versatile approach for classifying Neisseria gonorrhoeae lineages. The authors show that LIN codes capture both previously defined lineages and their relationships in a way that aligns with the species' phylogenetic structure. The compelling evidence presented, together with its integration into the PubMLST platform, underscores its strong potential to enhance epidemiological surveillance and advance our understanding of gonococcal population biology.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  19. Cortical layer 6b mediates state-dependent changes in brain activity and effects of orexin on waking and sleep

    This article has 12 authors:
    1. Elise J Meijer
    2. Marissa Mueller
    3. Lukas B Krone
    4. Tomoko Yamagata
    5. Anna Hoerder-Suabedissen
    6. Sian Wilcox
    7. Hannah Alfonsa
    8. Atreyi Chakrabarty
    9. Luiz Guidi
    10. Peter L Oliver
    11. Vladyslav V Vyazovskiy
    12. Zoltán Molnár
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study offers a valuable contribution to our understanding of the role of layer 6b cortical neurons in sleep-wake regulation, providing new insight into how this understudied neural population may regulate cortical arousal via orexin signaling. The evidence supporting these findings is solid, although somewhat constrained by limitations in the specificity of the genetic targeting strategy. Nonetheless, the work introduces new avenues for uncovering how the classical wake-promoting peptide, orexin, exerts its effects on the cortex.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  20. Post-translational modifications of microtubules are crucial for malaria parasite transmission

    This article has 10 authors:
    1. Kodzo Atchou
    2. Magali Roques
    3. Ruth Rehmann
    4. Reto Caldelari
    5. Melanie Schmid
    6. Simone Grossi
    7. Bianca Manuela Berger
    8. Torsten Ochsenreiter
    9. Friedrich Frischknecht
    10. Volker Heussler
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study provides valuable insights into microtubule remodeling during liver-stage Plasmodium berghei development, demonstrating that deletion of the alpha-tubulin C-terminal tail impairs parasite growth in mosquitoes and abolishes infection in HeLa cells. The work is technically ambitious, employing advanced microscopy, genetic mutants, and pharmacological approaches. However, key claims are only partially supported due to incomplete evidence linking tubulin modifications to microtubule dynamics and uncertain antibody-based PTM detection.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity