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  1. Cortical layer 6b mediates state-dependent changes in brain activity and effects of orexin on waking and sleep

    This article has 12 authors:
    1. Elise J Meijer
    2. Marissa Mueller
    3. Lukas B Krone
    4. Tomoko Yamagata
    5. Anna Hoerder-Suabedissen
    6. Sian Wilcox
    7. Hannah Alfonsa
    8. Atreyi Chakrabarty
    9. Luiz Guidi
    10. Peter L Oliver
    11. Vladyslav V Vyazovskiy
    12. Zoltán Molnár
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study offers a valuable contribution to our understanding of the role of layer 6b cortical neurons in sleep-wake regulation, providing new insight into how this understudied neural population may regulate cortical arousal via orexin signaling. The evidence supporting these findings is solid, although somewhat constrained by limitations in the specificity of the genetic targeting strategy. Nonetheless, the work introduces new avenues for uncovering how the classical wake-promoting peptide, orexin, exerts its effects on the cortex.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  2. Post-translational modifications of microtubules are crucial for malaria parasite transmission

    This article has 10 authors:
    1. Kodzo Atchou
    2. Magali Roques
    3. Ruth Rehmann
    4. Reto Caldelari
    5. Melanie Schmid
    6. Simone Grossi
    7. Bianca Manuela Berger
    8. Torsten Ochsenreiter
    9. Friedrich Frischknecht
    10. Volker Heussler
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study provides valuable insights into microtubule remodeling during liver-stage Plasmodium berghei development, demonstrating that deletion of the alpha-tubulin C-terminal tail impairs parasite growth in mosquitoes and abolishes infection in HeLa cells. The work is technically ambitious, employing advanced microscopy, genetic mutants, and pharmacological approaches. However, key claims are only partially supported due to incomplete evidence linking tubulin modifications to microtubule dynamics and uncertain antibody-based PTM detection.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  3. A novel mechanism for bacterial sporulation based on programmed peptidoglycan degradation

    This article has 9 authors:
    1. Carlos A Ramírez Carbó
    2. Oihane Irazoki
    3. Srutha Venkatesan
    4. Lauren JS Chen
    5. Haylie A Morales
    6. Assariel J Garcia Avila
    7. Hoi-Ling Cheung
    8. Felipe Cava
    9. Beiyan Nan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study identifies and partially characterises two proteins optimised for coordinated peptidoglycan degradation during two spore morphogenesis programs in the bacterium Myxococcus xanthus. The evidence supporting the conclusions is solid, although the description of the data is somewhat overstated. After some editing, the paper will be of interest to those studying peptidoglycan synthesis and reorganisation, which is a central aspect of microbial cell biology.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  4. Arrayed single-gene perturbations identify drivers of human anterior neural tube closure

    This article has 6 authors:
    1. Roya E Huang
    2. Giridhar M Anand
    3. Heitor C Megale
    4. Jason Chen
    5. Chudi Abraham-Igwe
    6. Sharad Ramanathan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study is a fundamental advance in the field of developmental biology and transcriptional regulation that demonstrates the use of hPSC-derived organoids to generate reproducible organoids to study the mechanisms that drive neural tube closure. The work is exceptional in its development of tools to use CRISPR interference to screen for genes that regulate morphogenesis in human PSC organoids. The additional characterization of the role of specific transcription factors in neural tube formation is solid. The work provides both technical advances and new knowledge on human development through embryo models.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  5. Resolving synaptic events using subsynaptically targeted GCaMP8 variants

    This article has 7 authors:
    1. Jiawen Chen
    2. Junhao Lin
    3. Kaikai He
    4. Luyi Wang
    5. Yifu Han
    6. Chengjie Qiu
    7. Dion Dickman
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this important study, the authors engineered and characterised novel genetically encoded calcium indicators (GECIs) and an analytical tool (CaFire) capable of reporting and quantifying various sub-synaptic events, including miniature synaptic events, with a speed and sensitivity approaching that of intracellular electrophysiological recordings. While the evidence supporting the improvements in the speed and accuracy of these tools is convincing, including additional information about key imaging parameters, the Bar8f experiments, and CaFire would strengthen the study. This work will be of interest to neurobiologists studying synaptic calcium dynamics in various model systems.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  6. Widespread cortical representations of innate behaviors in the mouse

    This article has 3 authors:
    1. Nicholas J Michelson
    2. Pankaj K Gupta
    3. Timothy H Murphy
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable work shows that subcortically-generated behaviors, like grooming, can have widespread representations in cortical activity. While the evidence is solid, additional analyses are necessary to strengthen the claims associated with outsized cortical representations of grooming onsets, as well as to address atypical grooming events. This work will be of interest to neuroscientists interested in how subcortically-generated behaviors are represented across the cortex.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  7. BuzzWatch: Uncovering Multi-scale Temporal Patterns in Mosquito Behavior Through Continuous Long-term Monitoring

    This article has 4 authors:
    1. Théo Maire
    2. Zhong Wan
    3. Louis Lambrechts
    4. Felix J.H. Hol
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This paper reports a useful low-cost platform for studying mosquito behaviors such as flight activity, sugar feeding, and host-seeking responses over the course of several weeks, and demonstrates key applications of this platform. While the authors provide a biological proof of principle, the evidence that supports the validation of the tracking algorithm is incomplete; it lacks biological replicates, independent confirmation of the tracking algorithm, and data on mosquito survival.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  8. Sulfation affects apical extracellular matrix organization during development of the Drosophila embryonic salivary gland tube

    This article has 6 authors:
    1. J Luke Woodward
    2. Jeffrey Matthew
    3. Rutuparna Joshi
    4. Vishakha Vishwakarma
    5. Ying Xiao
    6. SeYeon Chung
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This paper is important in demonstrating a requirement for sulfation in organizing apical extracellular matrix (aECM) during tubulogenesis in Drosophila melanogaster. The authors identify and characterize the organization of some of the first known components of the non-chitinous aECM in the Drosophila salivary gland tube, and these findings are supported by convincing data. This study would be of interest to developmental and cell biologists.

      [Editors' note: this paper was reviewed by Review Commons.]

    Reviewed by eLife, Review Commons

    This article has 12 evaluationsAppears in 2 listsLatest version Latest activity
  9. Evidence of off-target probe binding in the 10x Genomics Xenium v1 Human Breast Gene Expression Panel compromises accuracy of spatial transcriptomic profiling

    This article has 4 authors:
    1. Caleb Hallinan
    2. Hyun Joo Ji
    3. Steven L Salzberg
    4. Jean Fan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study identifies and characterizes probe binding errors in a widely used commercial platform for visualizing gene activity in tissue samples, discovering that at least 21 out of 280 genes in a human breast cancer panel are not accurately detected. The authors provide convincing evidence for their findings validated against multiple independent sequencing technologies and reference datasets. Given the broad adoption of this spatial gene detection platform in biomedical research, this work provides an essential quality control resource that will improve data interpretation across numerous studies.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  10. Single-cell transcriptome sequencing for opening the blood-brain barrier through specific mode electroacupuncture stimulation

    This article has 11 authors:
    1. Congcong Ma
    2. Zhaoxing Jia
    3. Tianxiang Jiang
    4. Qian Cai
    5. Jinding Yang
    6. Lin Gan
    7. Kecheng Qian
    8. Zixin Pan
    9. Qinyu Ye
    10. Mengyuan Dai
    11. Xianming Lin
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This paper reports a valuable discovery that specific-mode electroacupuncture (EA) transiently opens the blood-brain barrier (BBB) in rats. The evidence is solid but lacks functional validation of BBB permeability changes. The work will be of interest to medical scientists working in the field of electroacupuncture and drug delivery.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  11. The basolateral amygdala complex and perirhinal cortex represent focal and peripheral states of information processing in rats

    This article has 4 authors:
    1. Francesca S Wong
    2. A Simon Killcross
    3. R Fred Westbrook
    4. Nathan M Holmes
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important Research Advance builds on the authors' previous work delineating the roles of the rodent perirhinal cortex and the basolateral amygdala in first- and second-order learning. The convincing results show that serial exposure of non-motivationally relevant stimuli influences how those stimuli are encoded within the perirhinal cortex and basolateral amygdala when paired with a shock. This manuscript will be interesting for researchers in cognitive and behavioral neuroscience.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  12. Peripheral anatomy and central connectivity of proprioceptive sensory neurons in the Drosophila wing

    This article has 3 authors:
    1. Ellen Lesser
    2. Anthony Moussa
    3. John C Tuthill
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important work by Lesser et al provides a first and comprehensive description of Drosophila wing proprioceptors at an EM resolution. By linking peripheral neurons with information on their morphology and connectivity in the central nervous system, the authors provide new hypotheses and tools to study proprioceptive motor control of the wing in the fruit fly. The evidence and techniques supporting this work are solid, and this resource will contribute to connectome-based modeling of fly behavior.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  13. The hypoxic response extends lifespan through a bioaminergic and peptidergic neural circuit

    This article has 13 authors:
    1. Elizabeth S Kitto
    2. Shijiao Huang
    3. Mira Bhandari
    4. Cassie Tian
    5. Rebecca L Cox
    6. Safa Beydoun
    7. Emily Wang
    8. Danielle Shave
    9. Hillary A Miller
    10. Sarah A Easow
    11. Ella Henry
    12. Megan L Schaller
    13. Scott F Leiser
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This fundamental study identifies specific neural mechanisms through which HIF-1 signaling in ADF serotonergic neurons extends lifespan in C. elegans, revealing that downstream signaling in multiple types of neurons, as well as other neuromodulators like GABA, tyramine, and NLP-17, is required for this effect. The strength of the evidence is largely convincing, as the authors establish the necessity and causality of key neuronal components using multiple genetic tools and functional dissection in a well-validated model organism.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  14. Comprehensive Neural Representations of Naturalistic Stimuli through Multimodal Deep Learning

    This article has 5 authors:
    1. Mingxue Fu
    2. Guoqiu Chen
    3. Yijie Zhang
    4. Mingzhe Zhang
    5. Yin Wang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents a valuable application of a video-text alignment deep neural network model to improve neural encoding of naturalistic stimuli in fMRI. The authors found that models based on multimodal and dynamic embedding features of audiovisual movies predicted brain responses better than models based on unimodal or static features. The evidence supporting the claims is generally solid, with clear benchmarking against baseline models. The work will be of interest to researchers in cognitive neuroscience and AI-based brain modeling.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  15. Transcriptional responses to chronic oxidative stress require cholinergic activation of G-protein-coupled receptor signaling

    This article has 10 authors:
    1. Kasturi Biswas
    2. Caroline Moore
    3. Hannah Rogers
    4. Khursheed A Wani
    5. Read Pukkila-Worley
    6. Daniel P Higgins
    7. Amy K Walker
    8. Gregory P Mullen
    9. James B Rand
    10. Michael M Francis
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This useful study advances our understanding of how organisms respond to chronic oxidative stress. Using the nematode C. elegans, the authors identified key neuronal signaling molecules and their receptors that are required for stress signaling and survival. The evidence supporting the conclusions is solid, with rigorous genetics, stress response analysis, and transcriptional profiling. This research will be of broad interest to neuroscientists and researchers working in the field of oxidative stress regulation.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  16. Trpv4 mediates temperature induced sex change in ricefield eel

    This article has 4 authors:
    1. Zhi Yang
    2. Tingting Luo
    3. Yimin Zhang
    4. Yuhua Sun
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents useful findings on the molecular mechanisms driving female-to-male sex reversal in the ricefield eel (Monopterus albus) during aging, which would be of interest to biologists studying sex determination. The manuscript describes an interesting mechanism potentially underlying sex differentiation in M. albus. However, the current data are incomplete and would benefit from more rigorous experimental approaches.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  17. An expanded palette of bright and photostable organellar Ca2+ sensors

    This article has 12 authors:
    1. Agathe Moret
    2. Helen Farrants
    3. Ruolin Fan
    4. Kelsey G Zingg
    5. Bryon Silva
    6. Camilla Roselli
    7. Thomas G Oertner
    8. Christine E Gee
    9. Dafni Hadjieconomou
    10. Vidhya Rangaraju
    11. Eric R Schreiter
    12. Jaime de Juan-Sanz
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study introduces a new class of spectrally tunable, dye-based calcium sensors optimized for imaging in organelles with high calcium concentrations, such as the endoplasmic reticulum and mitochondria. The experimental evidence supporting the applicability of these sensors is convincing, with thorough validation in cultured cells and neurons. The work will be of high interest to researchers studying calcium signaling dynamics in subcellular compartments.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  18. Spectraplakin cooperates with noncentrosomal microtubule regulators to orient dendritic microtubules in Drosophila

    This article has 7 authors:
    1. Matthew Davies
    2. Neeraja Sanal
    3. Neele Wolterhoff
    4. Ulrike Gigengack
    5. Yitao Shen
    6. Ines Hahn
    7. Sebastian Rumpf
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      Davies et al. present a valuable study proposing that Shot can act as a molecular linker between microtubules and actin during dendrite pruning, suggesting an intriguing role in non-centrosomal microtubule organization. However, the experimental evidence is incomplete and does not robustly support these claims, and the lack of a cohesive model connecting the findings weakens the overall impact. While the data suggest that Shot, actin, and microtubule nucleation contribute to dendritic pruning, their precise interplay remains unresolved.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  19. Multiple modes of cholesterol translocation in the human Smoothened receptor

    This article has 4 authors:
    1. Prateek D Bansal
    2. Maia Kinnebrew
    3. Rajat Rohatgi
    4. Diwakar Shukla
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this useful study, the authors conducted a set of computational and experimental investigations of the mechanism of cholesterol transport in the smoothened (SMO) protein. The computational component integrated multiple state-of-the-art approaches such as adaptive sampling, free energy simulations, and Markov state modeling, providing support for the proposed mechanistic model, which is also consistent with the experimental mutagenesis data. However, substantial revisions are needed for the discussion of the computational results and interpretation of the literature to provide a more balanced and accurate perspective on cholesterol-mediated SMO regulation. In the current form, therefore, the strength of evidence of the study is considered incomplete.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  20. MIRO1 controls energy production and proliferation of vascular smooth muscle cells

    This article has 13 authors:
    1. Lan Qian
    2. Olha M Koval
    3. Benney T Endoni
    4. Denise Juhr
    5. Colleen S Stein
    6. Chantal Allamargot
    7. Li-Hsien Lin
    8. Deng-Fu Guo
    9. Kamal Rahmouni
    10. Ryan L Boudreau
    11. Jennifer Streeter
    12. William H Thiel
    13. Isabella M Grumbach
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study is valuable for understanding how dysfunctional mitochondria contribute to vascular diseases by investigating the influence of Miro1 on smooth muscle cell proliferation and neointima development. The solid findings collectively indicate that Miro1 regulates mitochondrial cristae architecture and the efficiency of the respiratory chain. Nevertheless, the analysis would benefit from a more thorough assessment of the relationship between Miro1-dependent mitochondrial defects and vascular smooth muscle cell proliferation.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity