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A list by Prachee Avasthi Recommended Reading

See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.

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  4. IntAct: a non-disruptive internal tagging strategy to study actin isoform organization and function

    1. M.C. van Zwam
    2. A. Dhar
    3. W. Bosman
    4. W. van Straaten
    5. S. Weijers
    6. E. Seta
    7. B. Joosten
    8. S. Palani
    9. K. van den Dries

    Reviewed by Review Commons, Arcadia Science

    5 evaluationsAppears in 3 listsLatest version Latest activity

    Prachee Avasthi Recommended Reading

    I’m certain I’ve highlighted this preprint before because it’s so interesting and useful but this is a new revision so check out the new analysis in figure 5. Tagging actin can affect its ability to polymerize, interact with its regulators, localize to the correct places, and perform its normal functions. This study identify an internal site to insert an epitope tag and tests those key features. As authors note, this is of particular interest to tag isoforms so their behaviors and functions can be teased apart.

  6. Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes

    1. Karl F Lechtreck
    2. Yi Liu
    3. Jin Dai
    4. Rama A Alkhofash
    5. Jack Butler
    6. Lea Alford
    7. Pinfen Yang

    • Curated by eLife

      Evaluation Summary:

      This paper is of broad interest to readers interested in motile cilia and cargo transport mediated by intraflagellar transport (IFT). It examines how radial spokes are trafficked into cilia by IFT, which represents a key process in the assembly of motile cilia. The authors demonstrate that an adaptor protein (ARMC2) is needed for association of radial spokes with the IFT machinery. They also find that the interaction of ARMC2/radial spokes with individual IFT trains (or particles) occurs in a stochastic manner rather than there being a specialized subset of trains specifically designated for a particular cargo. The results support the key claims in the paper.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife

    3 evaluationsAppears in 2 listsLatest version Latest activity

  7. Molecular mechanism of Arp2/3 complex inhibition by Arpin

    1. Fred E. Fregoso
    2. Trevor van Eeuwen
    3. Gleb Simanov
    4. Grzegorz Rebowski
    5. Malgorzata Boczkowska
    6. Austin Zimmet
    7. Alexis M. Gautreau
    8. Roberto Dominguez
    Appears in 1 listLatest version

  14. Long-range migration of centrioles to the apical surface of the olfactory epithelium

    1. Kaitlin Ching
    2. Jennifer T Wang
    3. Tim Stearns

    • Curated by eLife

      Evaluation Summary:

      Analyzing the long-distance migration of centrioles to the dendrite tip of multicilated olfactory neurons in mice, Ching et al. use expansion microscopy to show that centrioles migrate as clusters, which mature as they reach the apical surface. The super-resolution data are impressive and the claims are generally supported by the data. Although the manuscript is largely descriptive, it is an important addition to the field, and will be of broad interest to cell biologists.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife, preLights

    5 evaluationsAppears in 3 listsLatest version Latest activity

  15. Precise control of microtubule disassembly in living cells

    1. Grace Y Liu
    2. Shiau‐Chi Chen
    3. Gang‐Hui Lee
    4. Kritika Shaiv
    5. Pin‐Yu Chen
    6. Hsuan Cheng
    7. Shi‐Rong Hong
    8. Wen‐Ting Yang
    9. Shih‐Han Huang
    10. Ya‐Chu Chang
    11. Hsien‐Chu Wang
    12. Ching‐Lin Kao
    13. Pin‐Chiao Sun
    14. Ming‐Hong Chao
    15. Yian‐Ying Lee
    16. Ming‐Jer Tang
    17. Yu‐Chun Lin
    Appears in 1 listLatest version

  17. A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility

    1. Shu-Hsien Sheu
    2. Srigokul Upadhyayula
    3. Vincent Dupuy
    4. Song Pang
    5. Fei Deng
    6. Jinxia Wan
    7. Deepika Walpita
    8. H. Amalia Pasolli
    9. Justin Houser
    10. Silvia Sanchez-Martinez
    11. Sebastian E. Brauchi
    12. Sambashiva Banala
    13. Melanie Freeman
    14. C. Shan Xu
    15. Tom Kirchhausen
    16. Harald F. Hess
    17. Luke Lavis
    18. Yulong Li
    19. Séverine Chaumont-Dubel
    20. David E. Clapham
    Appears in 1 listLatest version
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