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  1. Robust de novo design of protein binding proteins from target structural information alone

    This article has 31 authors:
    1. Longxing Cao
    2. Brian Coventry
    3. Inna Goreshnik
    4. Buwei Huang
    5. Joon Sung Park
    6. Kevin M. Jude
    7. Iva Marković
    8. Rameshwar U. Kadam
    9. Koen H.G. Verschueren
    10. Kenneth Verstraete
    11. Scott Thomas Russell Walsh
    12. Nathaniel Bennett
    13. Ashish Phal
    14. Aerin Yang
    15. Lisa Kozodoy
    16. Michelle DeWitt
    17. Lora Picton
    18. Lauren Miller
    19. Eva-Maria Strauch
    20. Samer Halabiya
    21. Bradley Hammerson
    22. Wei Yang
    23. Steffen Benard
    24. Lance Stewart
    25. Ian A. Wilson
    26. Hannele Ruohola-Baker
    27. Joseph Schlessinger
    28. Sangwon Lee
    29. Savvas N. Savvides
    30. K. Christopher Garcia
    31. David Baker
    This article has no evaluationsAppears in 1 listLatest version
  2. Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex–dependent endocytosis

    This article has 3 authors:
    1. Brae M. Bigge
    2. Nicholas E. Rosenthal
    3. Prachee Avasthi
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This manuscript addresses the role of the Arp2/3 complex in endocytic retrieval of ciliary precursors from the plasma membrane for use in assembly of Chlamydomonas cilia, a topic of broad interest to cell biologists. The manuscript can serve as basis for future research addressing whether Arp2/3 affects cilium biogenesis solely via endocytic retrieval or through additional mechanisms, and whether these findings apply to species other than Chlamydomonas.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  3. Visualizing molecules of functional human profilin

    This article has 6 authors:
    1. Morgan L Pimm
    2. Xinbei Liu
    3. Farzana Tuli
    4. Jennifer Heritz
    5. Ashley Lojko
    6. Jessica L Henty-Ridilla
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This paper will be of interest to a broad audience of cell biologists and biochemists who study the cytoskeleton. It reports the development and rigorous characterization of a fully functional, fluorescently labeled version of profilin that can be used to visualize profilin's dynamic interactions in live cells. Owing to profilin's dual functions in regulating actin and microtubule assembly, this technological development will be a useful tool for a wide range of studies aimed at understanding the role of the cytoskeleton in driving fundamental cellular processes.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife, ASAPbio crowd review

    This article has 4 evaluationsAppears in 4 listsLatest version Latest activity
  4. Determinants shaping the nanoscale architecture of the mouse rod outer segment

    This article has 6 authors:
    1. Matthias Pöge
    2. Julia Mahamid
    3. Sanae S Imanishi
    4. Jürgen M Plitzko
    5. Krzysztof Palczewski
    6. Wolfgang Baumeister
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      Pöge at al. present a study of the rod outer segment (ROS). These are specialised cilia of rod photoreceptor cells, essential for sensing light cues and initiating the vision process. The authors apply cryo-FIB milling to generate highly preserved rod samples and report high-quality cryo-tomographic data providing new insights into the ultrastructure of the ROS. The work reveals potential molecular scaffolds both in the lumen of the membrane stacks and on the surface of the stack providing the structural basis for ROS crucial ordered ultrastructure. The data presented here will be highly valuable for the field of phototransduction.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  5. Systems-wide analysis revealed shared and unique responses to moderate and acute high temperatures in the green alga Chlamydomonas reinhardtii

    This article has 21 authors:
    1. Ningning Zhang
    2. Erin M. Mattoon
    3. Will McHargue
    4. Benedikt Venn
    5. David Zimmer
    6. Kresti Pecani
    7. Jooyeon Jeong
    8. Cheyenne M. Anderson
    9. Chen Chen
    10. Jeffrey C. Berry
    11. Ming Xia
    12. Shin-Cheng Tzeng
    13. Eric Becker
    14. Leila Pazouki
    15. Bradley Evans
    16. Fred Cross
    17. Jianlin Cheng
    18. Kirk J. Czymmek
    19. Michael Schroda
    20. Timo Mühlhaus
    21. Ru Zhang
    This article has no evaluationsAppears in 1 listLatest version