eLife reviews preprints in the life sciences and medicine.

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  2. TrueProbes: Quantitative Single-Molecule RNA-FISH Probe Design Improves RNA Detection

    1. Jason J Hughes
    2. Benjamin K Kesler
    3. John E Adams
    4. Blythe G Hospelhorn
    5. Gregor Neuert

    • Curated by eLife

      eLife Assessment

      This useful study introduces a computational pipeline for designing RNA in situ fluorescence hybridization probes that could improve the sensitivity and specificity of RNA detection in cells. While the approach is novel and the preliminary data suggestive, the evidence supporting a clear advantage over existing probe design strategies is incomplete. The work will be of interest to researchers developing or using molecular tools for imaging RNA in cells.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  3. Evolutionary Adaptations of IRG1 Refines Itaconate Synthesis and Mitigates Innate Immunometabolism Trade-offs

    1. Richard V Szeligowski
    2. Francois Miros
    3. Andres Saez
    4. Marisa DeCiucis
    5. Gunter P Wagner
    6. Hongying Shen

    • Curated by eLife

      eLife Assessment

      This important study addresses the timely and interesting question of how itaconate generation emerged in evolution, using taxonomic analysis of the gene and enzyme cis-aconitate decarboxylase (CAD). The authors provide solid evidence identifying three CAD branches in metazoans and showing that the early metazoan paleo-form indeed generates aconitate and is already linked to innate immunity. They further provide limited evidence suggesting that taxonomic differences in subcellular localisation of this enzyme may allow for innate immune signalling without compromising cellular energetics. The implications of the study will be of high interest to the field of innate host defence and immunometabolism.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  6. Generation of knock-in Cre and FlpO mouse lines for precise targeting of striatal projection neurons and dopaminergic neurons

    1. Eddy Albarran
    2. Akira Fushiki
    3. Anders Nelson
    4. David Ng
    5. Corryn Chaimowitz
    6. Laudan Nikoobakht
    7. Tanya Sippy
    8. Darcy S Peterka
    9. Rui M Costa

    • Curated by eLife

      eLife Assessment

      This important work has the potential to expand the repertoire of transgenic animals for systems neuroscience investigations across multiple fields. The generation of new reagents has the potential to open new directions in experimental design, and the Cas9-based approach for generating mice may provide additional benefits compared to existing BAC transgenic mouse lines. However, whereas some of the imaging data are compelling, quantitative analysis of transgene fidelity is incomplete, as it relies on a qualitative description of reporter XFP expression at low magnification, with some electrophysiological characterization.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  7. Enhancer-AAVs allow genetic access to oligodendrocytes and diverse populations of astrocytes across species

    1. John K Mich
    2. Smrithi Sunil
    3. Nelson Johansen
    4. Refugio A Martinez
    5. Jiatai Liu
    6. Bryan B Gore
    7. Joseph T Mahoney
    8. Mckaila Leytze
    9. Yoav Ben-Simon
    10. Darren Bertagnolli
    11. Ravi Bhowmik
    12. Yemeserach Bishaw
    13. Krissy Brouner
    14. Jazmin Campos
    15. Ryan Canfield
    16. Tamara Casper
    17. Nicholas P Donadio
    18. Nadezhda I Dotson
    19. Tom Egdorf
    20. Amanda Gary
    21. Shane Gibson
    22. Jeff Goldy
    23. Erin L Groce
    24. Kenta M Hagihara
    25. Daniel Hirschstein
    26. Han Hou
    27. Will D Laird
    28. Elizabeth Liang
    29. Luke V Loftus
    30. Nicholas Lusk
    31. Jocelin Malone
    32. Naomi X Martin
    33. Deja Monet
    34. Josh S Nagra
    35. Dakota Newman
    36. Nhan-Kiet Ngo
    37. Paul Olsen
    38. Victoria Omstead
    39. Ximena Opitz-Araya
    40. Aaron Oster
    41. Christina A Pom
    42. Lydia Potekhina
    43. Melissa Reding
    44. Christine Rimorin
    45. Augustin Ruiz
    46. Adriana E Sedeño-Cortés
    47. Nadiya V Shapovalova
    48. Michael Taormina
    49. Naz Taskin
    50. Michael Tieu
    51. Nasmil J Valera Cuevas
    52. Sharon W Way
    53. Natalie Weed
    54. Vonn Wright
    55. Zizhen Yao
    56. Thomas Zhou
    57. Delissa A McMillen
    58. Michael Kunst
    59. Medea McGraw
    60. Bargavi Thyagarajan
    61. Jack Waters
    62. Trygve E Bakken
    63. Nick Dee
    64. Shenqin Yao
    65. Kimberly A Smith
    66. Karel Svoboda
    67. Kaspar Podgorski
    68. Yoshiko Kojima
    69. Greg D Horwitz
    70. Hongkui Zeng
    71. Tanya L Daigle
    72. Ed S Lein
    73. Bosiljka Tasic
    74. Jonathan T Ting
    75. Boaz P Levi

    • Curated by eLife

      eLife Assessment

      This important study presents convincing findings on creating an exhaustive library of new enhancer-AAVs targeting astrocytes and oligodendrocytes with high potential for both basic and translational work, which will be of value to a large and growing community. However, the outdated description of glial biology in the Introduction, the overstated claims of utility in the Conclusion, and the loose stringency in the criteria used to assemble the library diminish the strengths of the claims. The work will be of interest to neuroscientists working on glial cell biology.

    Reviewed by eLife

    3 evaluationsAppears in 1 listLatest version Latest activity

  8. Functional connectivity, structural connectivity, and inter-individual variability in Drosophila melanogaster

    1. Takuto Okuno
    2. Alexander Woodward
    3. Hideyuki Okano
    4. Junichi Hata

    • Curated by eLife

      eLife Assessment

      This paper presents a collection of analyses relating structure and function in the whole-brain Drosophila EM connectome and whole-brain calcium imaging data. The linkage of detailed anatomical structure with population activity is of broad interest in circuit neuroscience in light of increasingly detailed brain maps, but the methods used made the evidence inadequate due to a lack of consideration of neurotransmitter identity and technical issues with the network analysis. The conclusions are useful for specific network observations, but a more thorough analysis of the anatomical and functional data is needed to support the overall claims.

    Reviewed by eLife

    8 evaluationsAppears in 1 listLatest version Latest activity
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