eLife

Latest preprint reviews

1. Pervasive duplication of tumor suppressors in Afrotherians during the evolution of large bodies and reduced cancer risk

   This article has 2 authors:

   1. Juan M Vazquez
   2. Vincent J Lynch

   Reviewed by eLife

2. Dendritic osmosensors modulate activity-induced calcium influx in oxytocinergic magnocellular neurons of the mouse PVN

   This article has 5 authors:

   1. Wanhui Sheng
   2. Scott W Harden
   3. Yalun Tan
   4. Eric G Krause
   5. Charles J Frazier

   Reviewed by eLife

3. Rapid recycling of glutamate transporters on the astroglial surface

   This article has 3 authors:

   1. Piotr Michaluk
   2. Janosch Peter Heller
   3. Dmitri A Rusakov

   This article has been curated by 1 group:

   • Curated by eLife

     Summary: In this study, Michaluk et al. examined the membrane dynamics of the main glial glutamate transporter GLT1 in hippocampal astrocytes, which was previously shown to shape synaptic transmission through regulating extracellular levels of glutamate. Using GLT1 tagged on its surface with a pH-sensitive fluorescent marker, GLT1-SEP, the authors performed (1) fluorescence recovery after photobleaching (FRAP) experiments to assess the basal and activity-dependent dynamics of surface GLT1-SEP and (2) super-resolution dSTORM imaging to determine the relationship between GLT1 and PSD-95, an excitatory synapse marker. A large proportion of surface GLT1-SEP underwent turnover with a surface lifetime of 22 s, whereas a smaller fraction (~25%) remained largely immobile, which was decreased upon increased activity. Notably, the cytoplasmic domain of GLT1-SEP was shown to attenuate the basal turnover of surface GLT1 and to facilitate its proximal localization to synapses; moreover, GLT1 cytoplasmic domain was required for activity-dependent increase in the mobile fraction.

     While previous studies using single molecule tracking have demonstrated a role for the lateral diffusion of GLT1 in controlling the recruitment of GLT1 near active synapses, the present study uses powerful optical approaches and analysis tools to access both the surface lateral mobility and the exchange between surface and intracellular pools of GLT1. Furthermore, characterization of the nanoscale organization of GLT1 relative to synapses and its dependence on the C-terminal domain of GLT1 is presented. Altogether, the results are interesting and valuable, and underscore the importance of the GLT1 C-terminus in the membrane turnover and in the activity-dependent lateral diffusion of the surface GLT1. Nevertheless, some of the conclusions are not strongly supported by the data shown.

     Reviewer #2 opted to reveal their name to the authors in the decision letter after review.

   Reviewed by eLife

4. Inhibitory control of frontal metastability sets the temporal signature of cognition

   This article has 5 authors:

   1. Vincent Fontanier
   2. Matthieu Sarazin
   3. Frederic M Stoll
   4. Bruno Delord
   5. Emmanuel Procyk

   This article has been curated by 1 group:

   • Curated by eLife

     Summary: Both reviewers found this study's findings to be interesting and novel, and they appreciated the integration of intrinsic timescale analysis, coding of behavioral signals, and exploration of mechanisms in a biophysical circuit model. However, they both raised serious concerns about methodological and interpretational aspects of the analyses which would need to be satisfactorily addressed in subsequent review. In consultation, both reviewers were in agreement with all points raised in the other's review. I view the two reviewers' requests and suggestions as appropriate and complementary, and all of them needed response.

     I highlight here two of the major concerns raised by the reviewers (but all raised by the reviewers merit addressing). First is the need for intrinsic timescale analysis to not be confounded by slowly varying changes in firing rate coding task variables (Point 1.2 of Reviewer 2). This is important for interpretation on intrinsic timescale and its correlation with task variable coding as a major result. Second is the interpretation and implementation of Hidden Markov Models to non-simultaneously recorded neurons (Points 6 of Reviewer 1 and 3 of Reviewer 2). Here again the HMM states may be driven by task variable coding which is not corrected for, which could confound interpretation of results as in terms of meta-stable states and its link to the circuit model. Furthermore, HMM analysis of the circuit model does not match its methodology for experimental data, but could be through non-simultaneous model spike trains, which the manuscript does not justify. I will add my own suggestion that perhaps both of these methodological data analysis concerns could potentially be addressed through comparison to the null model of a non-homogeneous Poisson process with firing rate given by the variable-coding PSTH. I do not consider it necessary to study a network model that performs the task, as suggested for consideration by Reviewer 1.

   Reviewed by eLife

5. Human Erbb2-induced Erk activity robustly stimulates cycling and functional remodeling of rat and human cardiomyocytes

   This article has 6 authors:

   1. Nicholas Strash
   2. Sophia DeLuca
   3. Geovanni L Janer Carattini
   4. Soon Chul Heo
   5. Ryne Gorsuch
   6. Nenad Bursac

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to scientists in the field of regenerative medicine. The authors compare effects of persistent lentiviral expression of various mitogens in cardiomyocytes in vitro. Technically experiments are of a very high standard, but the data are somewhat difficult to translate to the in vivo situation. The statistical analyses would have to be robust and sufficient for the conclusions to be supported by the data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

6. A low Smc flux avoids collisions and facilitates chromosome organization in Bacillus subtilis

   This article has 6 authors:

   1. Anna Anchimiuk
   2. Virginia S Lioy
   3. Florian Patrick Bock
   4. Anita Minnen
   5. Frederic Boccard
   6. Stephan Gruber

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript presents some intriguing data to support the notion that B. subtilis cells have tuned a variety of parameters related to SMC loading and translocation to ensure that individual complexes do not collide. This is likely an important but poorly understood aspect of condensins/SMCs, and as such represents a valuable contribution to the field and should be of interest to a broad set of readers.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Ligand sensing enhances bacterial flagellar motor output via stator recruitment

   This article has 5 authors:

   1. Farha Naaz
   2. Megha Agrawal
   3. Soumyadeep Chakraborty
   4. Mahesh S Tirumkudulu
   5. KV Venkatesh

   This article has been curated by 1 group:

   • Curated by eLife

     Summary: This is an interesting study reporting an increase in the rotation speed of the E. coli flagellar motor upon the sensing of a non-metabolizable glucose analog (2Dg) by the cell. The authors conclude that this increase is due to an increase in the number of torque-generating stator complexes that drive the motor. Knockout of the trg gene abolished this effect, suggesting that sensing of 2Dg by the Trg chemosensor is responsible. Involvement of membrane potential, the PTS pathway, and the chemotaxis response regulator CheY is ruled out. The manuscript is well-written, and the data are convincing. But the mechanism remains unclear.

     Reviewer #3 opted to reveal their name to the authors in the decision letter after review.

   Reviewed by eLife

8. Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA

   This article has 11 authors:

   1. Matthew T Parker
   2. Katarzyna Knop
   3. Vasiliki Zacharaki
   4. Anna V Sherwood
   5. Daniel Tomé
   6. Xuhong Yu
   7. Pascal GP Martin
   8. Jim Beynon
   9. Scott D Michaels
   10. Geoffrey J Barton
   11. Gordon G Simpson

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this study, the authors examined the function of the RNA-binding protein FPA through analyzing its protein interactome and its global impact on gene expression using a combined approach of Nanopore DRS, Helicos DRS, and short-read Illumina RNA-Seq. The combined datasets and new computational approaches developed by the authors permitted them to identify the predominant role of FPA in promoting poly(A) site choice. The authors further revealed that FPA mediates widespread premature cleavage and polyadenylation of transcripts of NLR genes, important plant immune regulators. Overall, this study suggests that control of transcription termination processes mediated by FPA provides an additional layer of the regulatory dynamics of NLRs in plant immune responses.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

9. Glypicans specifically regulate Hedgehog signaling through their interaction with Ihog in cytonemes

   This article has 9 authors:

   1. Eleanor Simon
   2. Carlos Jimenez-Jimenez
   3. Irene Seijo-Barandiaran
   4. Gustavo Aguilar
   5. David Sanchez-Hernandez
   6. Adrian Aguirre-Tamaral
   7. Laura Gonzalez-Mendez
   8. Pedro Ripoll
   9. Isabel Guerrero

   Reviewed by eLife

10. Artistoo, a library to build, share, and explore simulations of cells and tissues in the web browser

    This article has 2 authors:

    1. Inge MN Wortel
    2. Johannes Textor

    Reviewed by eLife