eLife

Latest preprint reviews

1. Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatment

   This article has 14 authors:

   1. Nicholas Tolman
   2. Taibo Li
   3. Revathi Balasubramanian
   4. Guorong Li
   5. Rebecca Pfeiffer
   6. Violet Bupp-Chickering
   7. Ruth A Kelly
   8. Marina Simón
   9. John Peregrin
   10. Christa Montgomery
   11. Bryan Jones
   12. W Daniel Stamer
   13. Jiang Qian
   14. Simon WM John

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides a fundamental advancement in our understanding of trabecular meshwork cell diversity and its role in eye pressure regulation and glaucoma using multimodal single-cell analysis, spatial validation, and functional testing that go beyond the current state-of-the-art. The study demonstrates that mitochondrial dysfunction, specifically in one of three distinct cell subtypes (TM3), contributes to elevated IOP in a genetic mouse model of glaucoma carrying a mutation in the transcription factor Lmx1b. While the identification of TM3 cells as metabolically specialized is compelling, there is somewhat limited evidence linking mitochondrial dysfunction to the Lmx1b mutation in TM3 cells.

   Reviewed by eLife

2. Passive muscle forces in Drosophila are large but insufficient to support a fly’s weight

   This article has 7 authors:

   1. Ninghan Wang
   2. Helene Babski
   3. Jonathan Elliot Perdomo
   4. Sarah Beth McMahan
   5. Arun Ramakrishnan
   6. Tirthabir Biswas
   7. Vikas Bhandawat

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study introduces a novel experimental and modeling framework to quantify passive joint torques in Drosophila, revealing that passive forces are insufficient to support body weight, contrary to prior assumptions based on larger insects. The approach is technically impressive, combining genetic silencing, kinematic tracking, and biomechanical modeling. However, the strength of evidence is incomplete, limited by concerns about the specificity of the genetic tools, simplifications in the mechanical model, and limited functional interpretation.

   Reviewed by eLife

3. Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

   This article has 7 authors:

   1. Jure Majnik
   2. Manon Mantez
   3. Sofia Zangila
   4. Stéphane Bugeon
   5. Léo Guignard
   6. Jean-Claude Platel
   7. Rosa Cossart

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental study presents a new method for longitudinally tracking cells in two-photon imaging data that addresses the specific challenges of imaging neurons in the developing cortex. It provides compelling evidence demonstrating reliable longitudinal identification of neurons across the second postnatal week in mice. The study should be of interest to development neuroscientists engaged in population-level recordings using two-photon imaging.

   Reviewed by eLife

4. A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

   This article has 8 authors:

   1. Alice Gros
   2. Jules Vanaret
   3. Valentin Dunsing-Eichenauer
   4. Agathe Rostan
   5. Philippe Roudot
   6. Pierre-François Lenne
   7. Léo Guignard
   8. Sham Tlili

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work describes the establishment of an image analysis pipeline for signal correction, segmentation and quantitative data analysis of multilayered organoid and tumoroid systems. The revised study is important for the field to address many practical challenges in deep-tissue visualization. The image analysis pipeline is well-designed and compelling.

   Reviewed by eLife

5. SETD2 suppresses tumorigenesis in a KRASG12C-driven lung cancer model, and its catalytic activity is regulated by histone acetylation

   This article has 11 authors:

   1. Ricardo J Mack
   2. Natasha M Flores
   3. Geoffrey C Fox
   4. Hanyang Dong
   5. Metehan Cebeci
   6. Simone Hausmann
   7. Tourkian Chasan
   8. Jill M Dowen
   9. Brian D Strahl
   10. Pawel K Mazur
   11. Or Gozani

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is a fundamental study providing molecular insight into how cross-talk between histone modifications regulates the histone H3K36 methyltransferase SETD2. The manuscript contains excellent quality data, and the conclusions are convincing and justified. This work will be of interest to many biochemists working in the field of chromatin biology and epigenetics.

   Reviewed by eLife

6. Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules

   This article has 10 authors:

   1. Samantha J Cassell
   2. Weiyan Li
   3. Simon Krautwald
   4. Maryam Khoshouei
   5. Yan Tony Lee
   6. Joyce Hou
   7. Wendy Guan
   8. Stefan Peukert
   9. Wilhelm Weihofen
   10. Jonathan R Whicher

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this important manuscript, Cassell and colleagues set out on a mechanistic and pharmacological exploration of an engineered chimeric small conductance calcium-activated potassium channel 2 (SK2). They show compelling evidence that the SK2 channel possesses a unique extracellular structure that modulates the conductivity of the selectivity filter, and that this structure is the target for the SK2 inhibitor apamin. The interpretations are sound and the writing is clear, and the manuscript was strengthened during review by providing more detailed information for the electrophysiological experiments and the structural analyses attempted, in addition to relating dilation of the filter to mechanisms of inactivation in other potassium channels. This high-quality study will be of interest to membrane protein structural biologists, ion channel biophysicists, and chemical biologists, and will help to inform future drug development targeting SK channels.

   Reviewed by eLife

7. Multiple pathways prevent bi-parental mitochondria transmission in C. elegans

   This article has 5 authors:

   1. Valentine Melin
   2. Justine Cailloce
   3. Fanny Husson
   4. Jorge Merlet
   5. Vincent Galy

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable work used molecular biology, cell biology, and genetic approaches to unravel individual genes and potential pathways that contribute to paternal mitochondrial inheritance using C. elegans as the model organism. Their microscopy method is cutting edge, with sufficient biological replicates, proper control, and appropriate statistics. These findings are convincing and are of general interest for understanding mitochondrial inheritance in C. elegans, which could have implications for understanding similar biological processes in other organisms.

   Reviewed by eLife

8. A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis

   This article has 6 authors:

   1. Ivana Petrovic
   2. Samit Desai
   3. Polina Isaikina
   4. Layara Akemi Abiko
   5. Anne Spang
   6. Stephan Grzesiek

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors investigate arrestin2-mediated CCR5 endocytosis in the context of clathrin and AP2 contributions. Using an extensive set of NMR experiments, and supported by microscopy and other biophysical assays, the authors provide compelling data on the roles of AP2 and clathrin in CCR5 endocytosis. This important work will appeal to an audience beyond those studying chemokine receptors, including those studying GPCR regulation and trafficking. The distinct role of AP2 and not clathrin will be of particular interest to those studying GPCR internalization mechanisms.

   Reviewed by eLife

9. Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

   This article has 8 authors:

   1. Çağrı Çevrim
   2. Béryl Laplace-Builhé
   3. Ko Sugawara
   4. Maria Lorenza Rusciano
   5. Nicolas Labert
   6. Jacques Brocard
   7. Alba Almazán
   8. Michalis Averof

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable technical advance in the long-term live imaging of limb regeneration at cellular resolution in Parhyale hawaiensis. The authors develop and carefully validate a method to continuously image entire regenerating legs over several days while minimizing photodamage and optimizing conditions for robust cell tracking, together with post-hoc in situ identification of cell types. The data are convincing, the methodology is rigorous and clearly documented, and the results will be of interest to researchers in regeneration biology, developmental biology, and advanced live imaging and cell tracking software development.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

10. Xcr1+ type 1 conventional dendritic cells are essential mediators for atherosclerosis progression

    This article has 17 authors:

    1. Tianhan Li
    2. Liaoxun Lu
    3. Juanjuan Qiu
    4. Xin Dong
    5. Le Yang
    6. Kexin He
    7. Yanrong Gu
    8. Binhui Zhou
    9. Tingting Jia
    10. Toby Lawrence
    11. Marie Malissen
    12. Guixue Wang
    13. Rong Huang
    14. Hui Wang
    15. Bernard Malissen
    16. Yinming Liang
    17. Lichen Zhang

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This manuscript by Li, Lu et al., presents important findings on the role of cDC1 in atherosclerosis and their influence on the adaptive immune system. Using Xcr1Cre-Gfp Rosa26LSL-DTA ApoE-/- mouse models, these data convincingly reveal an unexpected, non-redundant role of the XCL1-XCR1 axis in mediating cDC1 contributions to atherosclerosis.

    Reviewed by eLife