eLife

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1. We couldn't get details of this article at this time:
   10.1101/2020.12.14.422775

2. A 3D adult zebrafish brain atlas (AZBA) for the digital age

   This article has 9 authors:

   1. Justin W Kenney
   2. Patrick E Steadman
   3. Olivia Young
   4. Meng Ting Shi
   5. Maris Polanco
   6. Saba Dubaishi
   7. Kristopher Covert
   8. Thomas Mueller
   9. Paul W Frankland

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors present the first digital adult zebrafish brain anatomy atlas that includes ten highly informative markers for different brain regions and cell types. The authors utilize new clearing techniques to enable staining of the whole fixed zebrafish adult brains, and light sheet microscopy fluorescent imaging to obtain high resolution three-dimensional data. Several individual dataset for each marker are registered and aligned to an anatomical model using fluorescent cell nuclei and autofluorescence patterns by state of the art volume registration algorithms. The results appear to be of high quality. The authors also provide a detailed and state-of-the-art anatomical annotation of brain regions. Using ten different antibodies to fluorescently label different neuron types, stem cells and glia, proliferation zones, and some specific anatomical regions, the authors demonstrate the utility of their database. This will be a valuable resource for neurobiology research in zebrafish.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

3. Control of spinal motor neuron terminal differentiation through sustained Hoxc8 gene activity

   This article has 5 authors:

   1. Catarina Catela
   2. Yihan Chen
   3. Yifei Weng
   4. Kailong Wen
   5. Paschalis Kratsios

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to developmental geneticists interested in neuroscience, as it demonstrates how spinal motor neurons maintain their unique identities in adulthood after fate decisions are made in the embryo. The work here suggests that a Hox transcription factor acts as a terminal selector to control motor neuron identity, thus mirroring recent studies in C. elegans, and pointing towards this type of gene regulation as important in building diverse nervous systems.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Structure-guided microbial targeting of antistaphylococcal prodrugs

   This article has 14 authors:

   1. Justin J Miller
   2. Ishaan T Shah
   3. Jayda Hatten
   4. Yasaman Barekatain
   5. Elizabeth A Mueller
   6. Ahmed M Moustafa
   7. Rachel L Edwards
   8. Cynthia S Dowd
   9. Geoffrey C Hoops
   10. R Jeremy Johnson
   11. Paul J Planet
   12. Florian L Muller
   13. Joseph M Jez
   14. Audrey R Odom John

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This submission reports an approach to identify bacterial specific carboxyesterases that can be exploited to activate prodrugs of antibiotics in Staphylococcus aureus. The main premise is that charged functional groups found in some antibiotics prevent entry of these into bacteria, an effect that can be circumvented through esterification of the antibiotic to allow entry. Thereafter, activation of the antibiotic will occur in the bacterial cytoplasm through hydrolysis by bacterial esterases. This could lead to new antibiotic delivery strategies.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Rpl24Bst mutation suppresses colorectal cancer by promoting eEF2 phosphorylation via eEF2K

   This article has 11 authors:

   1. John RP Knight
   2. Nikola Vlahov
   3. David M Gay
   4. Rachel A Ridgway
   5. William James Faller
   6. Christopher Proud
   7. Giovanna R Mallucci
   8. Tobias von der Haar
   9. Christopher Mark Smales
   10. Anne E Willis
   11. Owen J Sansom

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this study, Knight and colleagues investigate the role of the ribosome and translational control in colorectal tumours. A mutation of a protein of the large ribosomal subunit, RPL24, is used to suppress tumours driven by two mutations found commonly in cancer, in APC and KRAS. The authors identify a mechanistic output of the RPL24 BST mutation, eEF2 phosphorylation, which they demonstrate is a major effector in inhibiting tumour cell translation and proliferation. By targeting the eEF2 kinase eEF2K, they restore protein synthesis in RPL24 mutant cells. The conclusion is well supported by the experimental data presented, which implies that translation elongation can be a potential therapeutic target of KRAS mutated CRC. Importantly, Rpl24Bst in wildtype intestine does not affect epithelial cell proliferation and differentiation, suggesting that translation elongation can be used as tumour-specific target.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

6. N501Y mutation of spike protein in SARS-CoV-2 strengthens its binding to receptor ACE2

   This article has 8 authors:

   1. Fang Tian
   2. Bei Tong
   3. Liang Sun
   4. Shengchao Shi
   5. Bin Zheng
   6. Zibin Wang
   7. Xianchi Dong
   8. Peng Zheng

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Tian et al. used a battery of biophysical techniques to compare wild-type SARS-CoV-2 with two variants (B.1.1.7 and B.1.351). The authors obtained evidence consistent with the receptor-binding domain (RBD) of the variants exhibiting higher affinity ACE2, providing a possible explanation for their higher transmission. However, the effects are modest, and further work will be needed to confirm that this difference explains the increased transmission of the variants. Despite the need for follow-up studies, the paper is among the first to show definitively that the N501Y mutation confers enhanced binding to ACE2.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife, ScreenIT

7. APOE4 is associated with elevated blood lipids and lower levels of innate immune biomarkers in a tropical Amerindian subsistence population

   This article has 15 authors:

   1. Angela R Garcia
   2. Caleb Finch
   3. Margaret Gatz
   4. Thomas Kraft
   5. Daniel Eid Rodriguez
   6. Daniel Cummings
   7. Mia Charifson
   8. Kenneth Buetow
   9. Bret A Beheim
   10. Hooman Allayee
   11. Gregory S Thomas
   12. Jonathan Stieglitz
   13. Michael D Gurven
   14. Hillard Kaplan
   15. Benjamin C Trumble

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors ask why the APOE4 allele has persisted, often at high frequencies, in human populations despite its associations to heart disease and Alzheimer's disease. They consider the hypothesis that APOE4 may be advantageous in a high pathogen and high physical environment settings (as opposed to a low pathogen industrial lifestyle) through an in-depth characterization of the Tsimane in Bolivia. The study is of broad interest with an insightful dataset; the conclusions are somewhat limited by the nature and current description and treatment of the data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. Modulation of fracture healing by the transient accumulation of senescent cells

   This article has 8 authors:

   1. Dominik Saul
   2. David G Monroe
   3. Jennifer L Rowsey
   4. Robyn Laura Kosinsky
   5. Stephanie J Vos
   6. Madison L Doolittle
   7. Joshua N Farr
   8. Sundeep Khosla

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a well conducted study that examines the potential role of senescent cells in fracture healing. Furthermore, the authors have used senolytic agents to examine if targeting cellular senescence will have a significant impact on fracture healing phenotype. The manuscript is succinctly written, and the methods and results are well described. These data provide the first demonstration for a potential role for cellular senescence in regulating fracture healing. The authors’ conclusions are largely supported by the experimental data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1, #2, and #3 agreed to share their names with the authors.)

   Reviewed by eLife

9. Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development

   This article has 6 authors:

   1. Maryam Izadi
   2. Eric Seemann
   3. Dirk Schlobinski
   4. Lukas Schwintzer
   5. Britta Qualmann
   6. Michael M Kessels

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript is of interest to scientists within the fields of actin cytoskeleton, cellular neurobiology and neurodevelopment. It explores how actin regulators are coordinated to trigger the formation of branches in neuronal dendritic arbor. Experiments are very well performed. Conclusions of the manuscript are convincingly supported by the results, although strict dependence of Cobl and Cobl-like in dendritic branch formation should perhaps be confirmed with additional experiments or tuned down. Results concerning the spatiotemporal relationship between the molecular players involved are more preliminary and few findings already published by the same group in previous articles should be expunged from this manuscript.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Matriptase generates a tissue damage response via promoting Gq signalling, leading to RSK and DUOX activation

    This article has 14 authors:

    1. MA Jiajia
    2. Claire A. Scott
    3. HO Ying Na
    4. Harsha Mahabaleshwar
    5. Katherine S. Marsay
    6. Changqing Zhang
    7. Christopher K. J. Teow
    8. NG Ser Sue
    9. Weibin Zhang
    10. Vinay Tergaonkar
    11. Lynda J. Partridge
    12. Sudipto Roy
    13. Enrique Amaya
    14. Tom J. Carney

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This manuscript unravels a detailed bipartite signaling mechanism, activation of which results in epithelial inflammation and cell motility. The paper is potentially of broad interest to cancer biologists and epithelial cell biologists. The data generated using the combination of genetic analyses, chemical inhibitors, and state-of-the-art confocal microscopy is of exceptionally high quality and supports the majority of the claims made in this paper.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife