eLife

Latest preprint reviews

1. Dynamics and variability in the pleiotropic effects of adaptation in laboratory budding yeast populations

   This article has 4 authors:

   1. Christopher W Bakerlee
   2. Angela M Phillips
   3. Alex N Nguyen Ba
   4. Michael M Desai

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The pleiotropic effects of beneficial mutations have been characterized in various settings, but it is less clear whether and how these pleiotropic patterns change over the course of evolution. Using a technically innovative and intensive experimental design with evolving yeast populations, the authors show that patterns of pleiotropy depend on the evolution environment and can change and vary substantially over relatively short timescales. They also find a surprising amount of variation among replicate populations that increases over time, so generalism or specialism is not deterministic. These technical and conceptual strengths were diminished by insufficient focus on the details of certain treatments that are demonstrative of these broader findings, making the take-home message somewhat unclear.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

2. Temporo-cerebellar connectivity underlies timing constraints in audition

   This article has 5 authors:

   1. Anika Stockert
   2. Michael Schwartze
   3. David Poeppel
   4. Alfred Anwander
   5. Sonja A Kotz

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors demonstrate deficits in perceptual tests related to fine-time perception in non-speech and speech sounds in a group of patients with stroke aphasia compared to a control group without a lesion. An area in left auditory cortex is defined that is essential for fine-time perception that is shown in a separate group of normal subjects to other areas including the cerebellum. The work in interesting in suggesting an anatomical basis for interaction between cortical and cerebellar system for perceptual timing.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Gaps in global wildlife trade monitoring leave amphibians vulnerable

   This article has 3 authors:

   1. Alice C Hughes
   2. Benjamin Michael Marshall
   3. Colin T Strine

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     These are pressing times for nature, standing alone the impact of multiple (human-based) ecological stressors. Wildlife trade is one of these stressors. And, although it is an acute one, it is the easiest solvable global ecological problem. The authors increase dramatically our understanding of legal and illegal trade of amphibians, and offer a wider methodology (however, and importantly, not necessarily a more complex one) to gain a deeper understanding of the causes and consequences) of amphibians' trade. The work will inspire in conservation biologists similar approaches to learn about the trade of other taxa.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

4. Structural insights into the activation of human calcium-sensing receptor

   This article has 14 authors:

   1. Xiaochen Chen
   2. Lu Wang
   3. Qianqian Cui
   4. Zhanyu Ding
   5. Li Han
   6. Yongjun Kou
   7. Wenqing Zhang
   8. Haonan Wang
   9. Xiaomin Jia
   10. Mei Dai
   11. Zhenzhong Shi
   12. Yuying Li
   13. Xiyang Li
   14. Yong Geng

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript reveals new molecular details about the human calcium sensing receptor (CaSR), a G protein-coupled receptor that maintains calcium homeostasis and is involved in pathological states. This study, together with other recent structural work on this molecule, will have important implications for the design of molecules that control CaSR activity and treat human disease.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

5. A distributed residue network permits conformational binding specificity in a conserved family of actin remodelers

   This article has 7 authors:

   1. Theresa Hwang
   2. Sara S Parker
   3. Samantha M Hill
   4. Meucci W Ilunga
   5. Robert A Grant
   6. Ghassan Mouneimne
   7. Amy E Keating

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes follow-up studies on a hit from a proteome-wide screen for peptides that can bind to the EVH1 domain of the ENAH protein, one of three highly similar Ena/VASP actin regulators. The hit investigated is from a protein called PCARE, which selectively binds to ENAH but not the other two members of the Ena/VASP family, EVL and VASP. The authors provide a good explanation for how this selectivity is achieved and develop a peptide, PCARE-Dual, that specifically binds ENAH more tightly, setting out the stage for developing potent and selective inhibitors of ENAH activity.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. HIV status alters disease severity and immune cell responses in Beta variant SARS-CoV-2 infection wave

   This article has 45 authors:

   1. Farina Karim
   2. Inbal Gazy
   3. Sandile Cele
   4. Yenzekile Zungu
   5. Robert Krause
   6. Mallory Bernstein
   7. Khadija Khan
   8. Yashica Ganga
   9. Hylton Rodel
   10. Ntombifuthi Mthabela
   11. Matilda Mazibuko
   12. Daniel Muema
   13. Dirhona Ramjit
   14. Thumbi Ndung'u
   15. Willem Hanekom
   16. Bernadett Gosnell
   17. COMMIT-KZN Team
   18. Moherndran Archary
   19. Kaylesh J Dullabh
   20. Jennifer Giandhari
   21. Philip Goulder
   22. Guy Harling
   23. Rohen Harrichandparsad
   24. Kobus Herbst
   25. Prakash Jeena
   26. Thandeka Khoza
   27. Nigel Klein
   28. Rajhmun Madansein
   29. Mohlopheni Marakalala
   30. Mosa Moshabela
   31. Kogie Naidoo
   32. Zaza Ndhlovu
   33. Kennedy Nyamande
   34. Nesri Padayatchi
   35. Vinod Patel
   36. Theresa Smit
   37. Adrie Steyn
   38. Richard J Lessells
   39. Emily B Wong
   40. Tulio de Oliveira
   41. Mahomed-Yunus S Moosa
   42. Gil Lustig
   43. Alasdair Leslie
   44. Henrik Kløverpris
   45. Alex Sigal

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript is of primary interest to readers in the field of infectious diseases especially the ones involved in COVID-19 research. The identification of immunological signatures caused by SARS-CoV-2 in HIV-infected individuals is important not only to better predict disease outcomes but also to predict vaccine efficacy and to potentially identify sources of viral variants. In here, the authors leverage a combination of clinical parameters, limited virologic information and extensive flow cytometry data to reach descriptive conclusions.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife, ScreenIT

7. Sticks and Stones, a conserved cell surface ligand for the Type IIa RPTP Lar, regulates neural circuit wiring in Drosophila

   This article has 3 authors:

   1. Namrata Bali
   2. Hyung-Kook (Peter) Lee
   3. Kai Zinn

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper claims to identify a long-sought ligand for the receptor protein tyrosine phosphatase Lar that mediates its functions in neuromuscular junction development, mushroom body development, and photoreceptor axon targeting. If correct, this would be of interest to many developmental neurobiologists, However, further evidence is needed to strongly support this claim.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. Genetically engineered mice for combinatorial cardiovascular optobiology

   This article has 15 authors:

   1. Frank K Lee
   2. Jane C Lee
   3. Bo Shui
   4. Shaun Reining
   5. Megan Jibilian
   6. David M Small
   7. Jason S Jones
   8. Nathaniel H Allan-Rahill
   9. Michael RE Lamont
   10. Megan A Rizzo
   11. Sendoa Tajada
   12. Manuel F Navedo
   13. Luis Fernando Santana
   14. Nozomi Nishimura
   15. Michael I Kotlikoff

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript by Lee and co-workers describes the development of 21 unique transgenic mouse lines that express optogenetic sensors and effectors in a cell lineage-specific fashion. The knock-in approach allows the sensors and effectors to be rapidly combined or moved to different backgrounds, such as genetic disease models. Such manipulations are often impractical when using a Cre-based system. This constitutes a vital advantage for many studies. The new mice described here will be very powerful tools to study physiology and alteration in disease models.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. Megalencephalic leukoencephalopathy with subcortical cysts is a developmental disorder of the gliovascular unit

   This article has 22 authors:

   1. Alice Gilbert
   2. Xabier Elorza-Vidal
   3. Armelle Rancillac
   4. Audrey Chagnot
   5. Mervé Yetim
   6. Vincent Hingot
   7. Thomas Deffieux
   8. Anne-Cécile Boulay
   9. Rodrigo Alvear-Perez
   10. Salvatore Cisternino
   11. Sabrina Martin
   12. Sonia Taïb
   13. Aontoinette Gelot
   14. Virginie Mignon
   15. Maryline Favier
   16. Isabelle Brunet
   17. Xavier Declèves
   18. Mickael Tanter
   19. Raul Estevez
   20. Denis Vivien
   21. Bruno Saubaméa
   22. Martine Cohen-Salmon

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study shows how astrocytic MCL1 can contribute to postnatal maturation of the brain vascular system. Since the development and physiological roles of perivascular astrocyte coverage are not well understood, this manuscript provides potentially important frame works and should be of interest to the broad fields of neuroscientists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

10. A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccines

    This article has 17 authors:

    1. Sara Feola
    2. Jacopo Chiaro
    3. Beatriz Martins
    4. Salvatore Russo
    5. Manlio Fusciello
    6. Erkko Ylösmäki
    7. Chiara Bonini
    8. Eliana Ruggiero
    9. Firas Hamdan
    10. Michaela Feodoroff
    11. Gabriella Antignani
    12. Tapani Viitala
    13. Sari Pesonen
    14. Mikaela Grönholm
    15. Rui MM Branca
    16. Janne Lehtiö
    17. Vincenzo Cerullo

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study describes an immunopeptidomic-based pipeline to discover new tumor antigens for the development of cancer vaccines. The pipeline is relatively straightforward and exploits molecular mimicry and tumor pathogen cross-reactive T-cells and would be interesting for cancer immunologists. If the utility of the pipeline were demonstrated in more diverse systems, including carcinogen-induced tumors and human settings, this work would provide an immediate impact to the immuno-oncology field and personalized cancer vaccine development.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife