eLife

Latest preprint reviews

1. An adhesion G protein-coupled receptor is required in cartilaginous and dense connective tissues to maintain spine alignment

   This article has 8 authors:

   1. Zhaoyang Liu
   2. Amro A Hussien
   3. Yunjia Wang
   4. Terry Heckmann
   5. Roberto Gonzalez
   6. Courtney M Karner
   7. Jess G Snedeker
   8. Ryan S Gray

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is an interesting and thorough study of the developmental roles of the G-coupled protein receptor Adgr6 in spine development that contributes both to the understanding of spine morphogenesis and the etiology of common types of scoliosis that are of unknown origin. Using conditional mouse knockouts, the authors dissect the contributions of Adgr6 in each spine-associated tissue. In addition to the use of state-of-the-art genetic tools, the authors show beautiful histological and tomography data illustrating developmental processes and phenotypes with great detail. Their results also implicate cAMP signaling and CREB activity in the regulation of mechanical properties of dense spine tissues.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

2. Muscle-specific economy of force generation and efficiency of work production during human running

   This article has 5 authors:

   1. Sebastian Bohm
   2. Falk Mersmann
   3. Alessandro Santuz
   4. Arno Schroll
   5. Adamantios Arampatzis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to scientists within the field of motor control and biomechanics studying human locomotion. The work provides evidence for the optimization of muscle function during locomotion depending on the specific biomechanical constraints. The overall methodology is sound and data are properly analyzed, although the in vivo measurements required a complex experimental setup together with sophisticated modeling which on the one hand conclusively support the key claims of the paper for the experiment within this paper, on the other hand weakens the generalizability of the results.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner

   This article has 14 authors:

   1. Lucas C Pantaleão
   2. Isabella Inzani
   3. Samuel Furse
   4. Elena Loche
   5. Antonia Hufnagel
   6. Thomas Ashmore
   7. Heather L Blackmore
   8. Benjamin Jenkins
   9. Asha A M Carpenter
   10. Ania Wilczynska
   11. Martin Bushell
   12. Albert Koulman
   13. Denise S Fernandez-Twinn
   14. Susan E Ozanne

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes the effects of maternal diet-induced obesity on lipid composition in maternal and fetal serum and the fetal heart, and in the fetal heart transcriptome. This study revealed sex-specific effects of obesity during pregnancy. The results presented provide insight into the still poorly understood processes influencing the long-term health of the fetus.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Loss of Tsc1 in cerebellar Purkinje cells induces transcriptional and translation changes in FMRP target transcripts

   This article has 10 authors:

   1. Jasbir Singh Dalal
   2. Kellen Diamond Winden
   3. Catherine Lourdes Salussolia
   4. Maria Sundberg
   5. Achint Singh
   6. Truc Thanh Pham
   7. Pingzhu Zhou
   8. William T Pu
   9. Meghan T Miller
   10. Mustafa Sahin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The main strength of the manuscript is the data sets generated by the cell type-specific RNA-seq and TRAP-seq in cerebellar PCs that lack Tsc1. In addition, the bioinformatic analysis revealed several interesting findings, including the observation that FMRP target RNAs are reduced in the Tsc1 mutant PCs and that the translational efficiency of these RNAs is actually increased, likely through compensatory mechanisms.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

5. Translation inhibitory elements from Hoxa3 and Hoxa11 mRNAs use uORFs for translation inhibition

   This article has 4 authors:

   1. Fatima Alghoul
   2. Schaeffer Laure
   3. Gilbert Eriani
   4. Franck Martin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Non-canonical pathways for regulating protein synthesis in animals serve important roles for controlling gene expression in critical developmental pathways. Homeobox (Hox) genes encode many mRNAs regulated at the level of translation. A general feature for many of these mRNAs has been the proposal they are regulated by Internal Ribosome Entry Sites and possess sequences in the 5'-untranslated regions of the mRNA that prevent canonical cap-dependent translation, termed "translation inhibitory elements". Here, the authors focus on two Hox mRNAs and find they use entirely different means to achieve the same end of repressing cap-dependent translation. Overall, the experiments support the major conclusions drawn by the authors, and nail down mechanisms that have been left unresolved since the Hox mRNAs were first discovered to be regulated at the level of translation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Natural Selection is Unlikely to Explain Why Species Get a Thin Slice of π

   This article has 1 author:

   1. Vince Buffalo

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript revisits an enduring and central question in population genetics known as Lewontin's paradox: that in contrast to the prediction of the field's null model, which suggests that levels of neutral genetic diversity should be proportional to the census population size, in reality, census population sizes span several orders of magnitude more than the approximately three orders of magnitude spanned by levels of genetic diversity. The manuscript provides a nice review of previous work as well as thought-provoking novel analyses. There are also several issues that make it difficult to interpret the new results.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #4 agreed to share their names with the authors.)

   Reviewed by eLife

7. HIF1α is required for NK cell metabolic adaptation during virus infection

   This article has 11 authors:

   1. Francisco Victorino
   2. Tarin M Bigley
   3. Eugene Park
   4. Cong-Hui Yao
   5. Jeanne Benoit
   6. Li-Ping Yang
   7. Sytse J Piersma
   8. Elvin J Lauron
   9. Rebecca M Davidson
   10. Gary J Patti
   11. Wayne M Yokoyama

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     By using mice lacking the hypoxia inducible factor-1α (HIF1α) in NK cells, the study unravels a previously unknown function of this transcription factor in virus control by NK cells. Mechanistically, the authors provided evidence that HIF1α supports survival of NK cells through an efficient glucose metabolism required for optimal NK cell response to viral infection.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast

   This article has 8 authors:

   1. Pascal D Odermatt
   2. Teemu P Miettinen
   3. Joël Lemière
   4. Joon Ho Kang
   5. Emrah Bostan
   6. Scott R Manalis
   7. Kerwyn Casey Huang
   8. Fred Chang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This article contributes to the fundamental understanding of how a cell grows. It provides a broadly applicable method for dry mass measurement of single cells and, using it, it describe how cell density varies accross the cell division cycle. The key finding of this article is the fact that growth in mass and volume seem to be generally uncoupled, leading to significant density changes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. Disrupting cortico-cerebellar communication impairs dexterity

   This article has 8 authors:

   1. Jian-Zhong Guo
   2. Britton A Sauerbrei
   3. Jeremy D Cohen
   4. Matteo Mischiati
   5. Austin R Graves
   6. Ferruccio Pisanello
   7. Kristin M Branson
   8. Adam W Hantman

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The present paper investigated the role of cortico-cerebellar loops in motor control with high density physiological recordings and by using optogenetics to perturb responses of precerebellar neurons in the pontine nuclei during reaching. The study adds to a long line of work supporting the view that the cortico-cerebellar pathway is required for fine motor control. The experiments are well performed, but a number of revisions in analysis and presentation are required.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Tonic interferon restricts pathogenic IL-17-driven inflammatory disease via balancing the microbiome

    This article has 14 authors:

    1. Isabelle J Marié
    2. Lara Brambilla
    3. Doua Azzouz
    4. Ze Chen
    5. Gisele V Baracho
    6. Azlann Arnett
    7. Haiyan S Li
    8. Weiguo Liu
    9. Luisa Cimmino
    10. Pratip Chattopadhyay
    11. Gregg Silverman
    12. Stephanie S Watowich
    13. Bernard Khor
    14. David E Levy

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This manuscript describes a critical function for interferons (IFNs) in suppression of spontaneous autoinflammatory disease. The inflammatory disease is seen in mice lacking all types (I, II, III) of IFN signaling and is ameliorated by deficiency in interleukin-17 as well as by antibiotic treatment. The latter result implies a role for the microbiota in disease pathogenesis, but it remains unclear whether or how IFNs regulate the microbiota composition, whether this occurs across genetic backgrounds or housing conditions, or how exactly IFN deficiency leads to inflammatory disease. Nevertheless this work demonstrates a critical regulatory function for tonic IFN signaling in suppressing autoinflammation that serves as a foundation for future studies and will be of interest to a broad audience of immunologists.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife