eLife

Latest preprint reviews

1. Multi-tract multi-symptom relationships in pediatric concussion

   This article has 7 authors:

   1. Guido I Guberman
   2. Sonja Stojanovski
   3. Eman Nishat
   4. Alain Ptito
   5. Danilo Bzdok
   6. Anne L Wheeler
   7. Maxime Descoteaux

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript aims to address an important issue in the study of concussion: both the brain damage caused by concussion, as well as the behavioral symptoms that result vary widely across individuals. The study uses novel and interesting methods to relate multi-variate diffusion MRI data with multi-variate symptom-related data. The methods of analysis are sophisticated and well-executed and the results are quite interesting. The methods developed here could have broad impact in their application to the many other neurological diseases that have heterogeneous outcomes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

2. Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes

   This article has 6 authors:

   1. Stefan T Huber
   2. Edin Sarajlic
   3. Roeland Huijink
   4. Felix Weis
   5. Wiel H Evers
   6. Arjen J Jakobi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Sample preparation for single-particle electron cryo-microscopy (cryo-EM) remains a bottleneck of this technique. The sample ice thickness cannot be accurately controlled, molecules may display strongly preferred orientations that make more elaborate data collection schemes necessary, and the sample may degrade at the air-water interface before it is finally frozen. In their pioneering work, the authors describe a prototype of a new microfluidic device that addresses some of these problems, including a refreshingly objective and critical discussion about the pros and cons of this novel approach. While some development will be required for this method to become mainstream, it has the potential to become a powerful alternative to the conventional workflow of single-particle cryo-EM, enabling full automation and making sample preparation highly reproducible.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

3. Diverse mating phenotypes impact the spread of wtf meiotic drivers in Schizosaccharomyces pombe

   This article has 8 authors:

   1. José Fabricio López Hernández
   2. Rachel M Helston
   3. Jeffrey J Lange
   4. R Blake Billmyre
   5. Samantha H Schaffner
   6. Michael T Eickbush
   7. Scott McCroskey
   8. Sarah E Zanders

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Meiotic drivers are selfish elements that distort segregation to be over-represented in offspring of heterozygotes. Multiple meiotic drive elements are known in the yeast Schizosaccharomyces pombe, which can seem puzzling as this fungus has long been thought to undergo moslty same-clone mating because of its mating-type switching system. This manuscript reports theoretical and experimental analyses suggesting that the outcrossing rate can be high enough in this species to explain the spread of multiple meiotic drive elements. The findings support the emerging view that homothallic fungi can undergo quite high rates of outcrossing, which is also in agreement with evolutionary considerations on the evolution of mating types. This study can thus be of high relevance for scientists studying meiotic drivers and/or mating systems and their evolution.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

4. mRNA vaccine-induced T cells respond identically to SARS-CoV-2 variants of concern but differ in longevity and homing properties depending on prior infection status

   This article has 8 authors:

   1. Jason Neidleman
   2. Xiaoyu Luo
   3. Matthew McGregor
   4. Guorui Xie
   5. Victoria Murray
   6. Warner C Greene
   7. Sulggi A Lee
   8. Nadia R Roan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work will be of broad interest to those studying adaptive immunity to SARS-CoV-2, particularly with a focus on T cell immunology. The study confirms that mRNA vaccine-elicited T cell responses maintain recognition of peptides derived from VOC, and provides phenotypic characterisation of spike-specific T cells from both convalescent and infection-naive subjects.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

5. Novel mechanistic insights into the role of Mer2 as the keystone of meiotic DNA break formation

   This article has 11 authors:

   1. Dorota Rousová
   2. Vaishnavi Nivsarkar
   3. Veronika Altmannova
   4. Vivek B Raina
   5. Saskia K Funk
   6. David Liedtke
   7. Petra Janning
   8. Franziska Müller
   9. Heidi Reichle
   10. Gerben Vader
   11. John R Weir

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Using a combination of biochemical approaches and yeast genetics, the authors study the function of the DNA double-strand break factor Mer2. The authors show that Mer2 interacts with a meiotic chromosome axis factor (Hop1), nucleosomes, the nucleosome-binding protein Spp1, and the double-strand break factor Mre11 to serve as a "keystone" for meiotic DNA break formation. These findings represent an important step forward in understanding the functions of this highly conserved protein in meiosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

6. Correct regionalization of a tissue primordium is essential for coordinated morphogenesis

   This article has 3 authors:

   1. Yara E Sánchez-Corrales
   2. Guy B Blanchard
   3. Katja Röper

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Sanchez et al investigate how morphogenetic movements driving epithelial tube formation are patterned to occur with the correct spatiotemporal dynamics, a fundamental question in developmental biology. By correlating dynamic patterns of transcription factor expression with rigorous, quantitative analyses of cell behaviors across the salivary gland primordium, their results suggest Hkb and Fkh transcription factor patterning induces switches in cell behaviors at fixed positions to promote continued morphogenesis of the tubular structure. This mechanism is likely to be more generally important for the development of complex tubular organs.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

7. The E3 ubiquitin ligase mindbomb1 controls planar cell polarity-dependent convergent extension movements during zebrafish gastrulation

   This article has 6 authors:

   1. Vishnu Muraleedharan Saraswathy
   2. Akshai Janardhana Kurup
   3. Priyanka Sharma
   4. Sophie Polès
   5. Morgane Poulain
   6. Maximilian Fürthauer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes a novel role of the E3 ubiquitin ligase Mindbomb1 (Mib1), a known key regulator of Notch signaling, in regulating convergent extension movements of the zebrafish gastrula, which are dependent on planar cell polarity (PCP) signaling. The authors show that the ability of Mib1 to modulate PCP is totally dependent on the receptor tyrosine kinase Ryk via endocytosis. This paper will be of interest to scientists studying cell signaling and cell movement.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. SOX4 facilitates PGR protein stability and FOXO1 expression conducive for human endometrial decidualization

   This article has 17 authors:

   1. Pinxiu Huang
   2. Wenbo Deng
   3. Haili Bao
   4. Zhong Lin
   5. Mengying Liu
   6. Jinxiang Wu
   7. Xiaobo Zhou
   8. Manting Qiao
   9. Yihua Yang
   10. Han Cai
   11. Faiza Rao
   12. Jingsi Chen
   13. Dunjin Chen
   14. Jinhua Lu
   15. Haibin Wang
   16. Aiping Qin
   17. Shuangbo Kong

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Huang et al have identified SOX4 as a key regulatory factor that controls prolactin and FOXO1 transcription factor which in turn regulate decidualization, an important process in embryo development. Further, they have identified that SOX4 also regulates progesterone receptor and dysregulated SOX4-progesterone and ubiquitin ligase HERC4can lead to embryo implantation failure thus explaining the molecular basis of recurrent implantation failure in humans. Overall the study is interesting and the data are very strong. Some concerns noted were the use of immortalized stromal cells, and incomplete nature of studies with human endometrial stroll cells from endometriosis patients, and lack of sufficient discussion in some parts of the text, and whether specific progesterone isofoms are involved downstream of SOX4.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain

   This article has 15 authors:

   1. Elizabeth L Wagner
   2. Jun-Sub Im
   3. Stefano Sala
   4. Maura I Nakahata
   5. Terence E Imbery
   6. Sihan Li
   7. Daniel Chen
   8. Katherine Nimchuk
   9. Yael Noy
   10. David W Archer
   11. Wenhao Xu
   12. George Hashisaki
   13. Karen B Avraham
   14. Patrick W Oakes
   15. Jung-Bum Shin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study investigates a process by which hair cell stereocilia, the sensory structures that respond to sound in the hearing organ and to head motion or tilt in the vestibular organ, can recover from damage-induced gaps in their actin core, possibly allowing for the rescue of transient hearing loss after exposure to noise. This manuscript will be of strong interest to the inner ear field as well as readers with broader interest in actin cytoskeleton dynamics. Although meticulous controls, a combination of molecular, histological and functional studies and an innovative mouse model generally support the major conclusions of this study, additional controls are needed to confirm the mechanistic claims made in the manuscript.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. An entropic safety catch controls hepatitis C virus entry and antibody resistance

    This article has 16 authors:

    1. Lenka Stejskal
    2. Mphatso D Kalemera
    3. Charlotte B Lewis
    4. Machaela Palor
    5. Lucas Walker
    6. Tina Daviter
    7. William D Lees
    8. David S Moss
    9. Myrto Kremyda-Vlachou
    10. Zisis Kozlakidis
    11. Giulia Gallo
    12. Dalan Bailey
    13. William Rosenberg
    14. Christopher JR Illingworth
    15. Adrian J Shepherd
    16. Joe Grove

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      HCV is unique in its glycoprotein structure, complex receptor usage and its unusual persistence for a (+)RNA virus. This is a well done study that explains a number of observations regarding receptor usage and how HCV may evade antibody control via HVR1 due to its disordered nature, enable mutation to continually evade antibody responses. This manuscript should be of substantial interest to those in the fields of virus entry, vaccination against human viruses, and the study of how intrinsically disordered regions can play regulatory roles.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife