eLife

Latest preprint reviews

1. Endurance exercise ameliorates phenotypes in Drosophila models of spinocerebellar ataxias

   This article has 5 authors:

   1. Alyson Sujkowski
   2. Kristin Richardson
   3. Matthew V Prifti
   4. Robert J Wessells
   5. Sokol V Todi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is important work trying to decipher some of the potential benefits and pathways from exercise. SCA2 was most impacted by exercise, and this correlated with Sestrin increases that proportionally led to decreases in the disease causing SCA2 protein (but not SCA3 so much). Sestrin alone was able to affect the disease severity in SCA2 flies, via the interaction with mTOR and the autophagy pathway.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

2. Nanoscale architecture and coordination of actin cores within the sealing zone of human osteoclasts

   This article has 9 authors:

   1. Marion Portes
   2. Thomas Mangeat
   3. Natacha Escallier
   4. Ophélie Dufrancais
   5. Brigitte Raynaud-Messina
   6. Christophe Thibault
   7. Isabelle Maridonneau-Parini
   8. Christel Vérollet
   9. Renaud Poincloux

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript, the authors studied, using different types of microscopy, the structure and function of podosomes in primary human osteoclasts, providing interesting new insights into the organization and dynamics of the osteoclast sealing zone. The authors describe the general structure of the actin rings of osteoclasts and explore the dynamics of actin and associated proteins in the actin ring, proposing a model in which the function of podosomes during bone resorption is accomplished through the coordination of islets of the actin core and not through the global coordination of all podosome subunits that form the sealing zone. This article has the potential to generate a significant impact in the field of osteoclast biology. Notably, this study could also expand the understanding of matrix degradation/invasion in other tissues.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. In vivo intraoral waterflow quantification reveals hidden mechanisms of suction feeding in fish

   This article has 4 authors:

   1. Pauline Provini
   2. Alexandre Brunet
   3. Andréa Filippo
   4. Sam Van Wassenbergh

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     How do fish suck food underwater? Using new artificial food particles that are radio opaque and naturally buoyant, Provini et al. imaged the roller-coaster ride that food particles make being sucked-in from outside to inside the fish, using 3D stereo high-speed fluoroscopy. The recordings show fish to have an intriguing ability to generate flows that center the food particles as they enter the buccal cavity that carries them from the outside to the center of the digestive tract. Remarkably, the flow patterns in the mouth that accomplish this seem to differ between the two species of fish studied, although samples sizes are small at present. These new insights will interest biologists working on suction feeding mechanisms ranging from millimeter-sized carnivorous water plants, tadpoles and fish larvae, to large fish and marine mammals, and even gigantic whales. Bioinspired engineers designing rapid underwater suction apparatuses may benefit from harnessing the new insights to elegantly center items of interest.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. T cells discriminate between groups C1 and C2 HLA-C

   This article has 6 authors:

   1. Malcolm J W Sim
   2. Zachary Stotz
   3. Jinghua Lu
   4. Paul Brennan
   5. Eric O Long
   6. Peter D Sun

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this study, the authors use structural, functional and immunopeptidomics analysis to provide insights into how HLA-C C1/C2 dimorphism impacts T cell recognition. This knowledge is important in immunotherapies targeting HLA-C-specific T cells. Overall, the experiments are well-performed, and the conclusions are largely supported by the presented data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members

   This article has 5 authors:

   1. Jakub Gemperle
   2. Thomas S Harrison
   3. Chloe Flett
   4. Antony D Adamson
   5. Patrick T Caswell

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work reports a genome editing strategy that enables blocking and tetracycline-controlled re-expression of fluorescently-tagged proteins from endogenous loci. The authors combine this with the photoactivatable tet-on/off system, a knocksideways approach, as well as the auxin-inducible degron system to improve spatial and temporal control of gene expression. They demonstrate the applicability of the methods by studying the localization, function and protein-expression dynamics of the Rab11-family of small GTPases. Using these approaches, the authors discover subtle differences in the localization as well as expression and degradation kinetics of Rab11a/b/Rab25. The work will be of broad interest to molecular and cell biologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. Rapid, Reference-Free human genotype imputation with denoising autoencoders

   This article has 7 authors:

   1. Raquel Dias
   2. Doug Evans
   3. Shang-Fu Chen
   4. Kai-Yu Chen
   5. Salvatore Loguercio
   6. Leslie Chan
   7. Ali Torkamani

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The paper describes a novel neural-network-based strategy for imputing unmeasured genotypes, which is standard part of most association testing pipelines. The method, while computationally intensive to train, can perform the imputation quickly and conveniently, and has the potential to be a practically-appealing alternative to existing methods. However, further work will be required to realize this potential, and further data are required to support the accuracy of the method.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Intrinsic timescales as an organizational principle of neural processing across the whole rhesus macaque brain

   This article has 5 authors:

   1. Ana MG Manea
   2. Anna Zilverstand
   3. Kamil Ugurbil
   4. Sarah R Heilbronner
   5. Jan Zimmermann

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Neural activity measured in both electrophysiological and functional neuroimaging experiments are often temporally correlated, and the timescales of such correlation in ongoing neural activity, or intrinsic neural timescales, show a hierarchical pattern across the cortical surface. The present study establishes a close link between these timescales and functional connectivity in the brains of non-human primates, suggesting that temporal autocorrelation is an important organizing feature of large-scale neural activity.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Listeria monocytogenes requires cellular respiration for NAD+ regeneration and pathogenesis

   This article has 13 authors:

   1. Rafael Rivera-Lugo
   2. David Deng
   3. Andrea Anaya-Sanchez
   4. Sara Tejedor-Sanz
   5. Eugene Tang
   6. Valeria M Reyes Ruiz
   7. Hans B Smith
   8. Denis V Titov
   9. John-Demian Sauer
   10. Eric P Skaar
   11. Caroline M Ajo-Franklin
   12. Daniel A Portnoy
   13. Samuel H Light

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In their study, Rivera-Luogo et al. investigate the main role of respiration in Listeria monocytogenes pathogenicity, whether it is more important for redox balance control (ratio of balance NAD+/NADH) or generation of proton motive force. Drawing on results from an elegant heterologous NOX system supporting regeneration of NAD without producing proton motive force, the authors propose that NAD regeneration, i.e. redox balance, is more important for Listeria than proton motive force as a result of respiration. This is clearly a new way of perceiving respiration that should be of interest broadly to the microbiology community and broader readership.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation

   This article has 8 authors:

   1. Jiale Du
   2. Sandor Babik
   3. Yanfeng Li
   4. Kirandeep K Deol
   5. Stephen J Eyles
   6. Jasna Fejzo
   7. Marco Tonelli
   8. Eric Strieter

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study identifies a cryptic ubiquitin-binding site on the proteasome-associated deubiquitinase UCH37 and reveals that branched ubiquitin chains are bound and processed differently from mono-ubiquitin modifications. The authors use a variety of elegant biochemical and biophysical approaches to characterize this new binding site, and the conclusions are very well supported by the experimental data. These findings provide important new insights into the cleavage of branched ubiquitin chains during protein degradation by the 26S proteasome, therefore represent a critical advance to the ubiquitin-proteasome field and our understanding of how ubiquitin signaling regulates protein turnover in the cell, and will be of interest to a broad audience.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Expansion and contraction of resource allocation in sensory bottlenecks

    This article has 3 authors:

    1. Laura R Edmondson
    2. Alejandro Jiménez Rodríguez
    3. Hannes P Saal

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The paper develops a mathematical approach to study the allocation of cortical area to sensory representations in the presence of resource constraints. The theory is applied to study sensory representations in the somatosensory system. This problem is largely unexplored, the results are novel and can be of interest to experimental and theoretical neuroscientists.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife