eLife

Latest preprint reviews

1. Atmospheric particulate matter aggravates CNS demyelination through involvement of TLR-4/NF-kB signaling and microglial activation

   This article has 14 authors:

   1. Bing Han
   2. Xing Li
   3. Ruo-Song Ai
   4. Si-Ying Deng
   5. Ze-Qing Ye
   6. Xin Deng
   7. Wen Ma
   8. Shun Xiao
   9. Jing-Zhi Wang
   10. Li-Mei Wang
   11. Chong Xie
   12. Yan Zhang
   13. Yan Xu
   14. Yuan Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors investigated Atmospheric Particulate Matter (PM) , a mimetic of current air pollution and how it affects myelination in the central nervous system (CNS) using an animal model of demyelination. They showed that the PM caused changes in myelination that associated with microglial activation and markers of inflammation. In vitro modeling indicates PM can activate microglial via the TLR4/NFkB signaling pathway.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. Kv3.3 subunits control presynaptic action potential waveform and neurotransmitter release at a central excitatory synapse

   This article has 10 authors:

   1. Amy Richardson
   2. Victoria Ciampani
   3. Mihai Stancu
   4. Kseniia Bondarenko
   5. Sherylanne Newton
   6. Joern R Steinert
   7. Nadia Pilati
   8. Bruce P Graham
   9. Conny Kopp-Scheinpflug
   10. Ian D Forsythe

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors make a compelling case for a special type of potassium channel (Kv3.3) in the control of the presynaptic AP spike waveforms. Importantly, mice that lack Kv3.3 showed auditory response deficits, including increases in pre-synaptic AP halfwidth, AP latency, AP jitter and spontaneous activity. The in vivo recordings are impressive. The study contains an extensive data set and makes a compelling argument for the uniquely important role for Kv3.3 in synaptic transmission. Overall, the findings will significantly advance our understanding of the calyx of Held and the neural circuit of sound processing, as well as the functions of Kv3 channels.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Boosting targeted genome editing using the hei-tag

   This article has 8 authors:

   1. Thomas Thumberger
   2. Tinatini Tavhelidse-Suck
   3. Jose Arturo Gutierrez-Triana
   4. Alex Cornean
   5. Rebekka Medert
   6. Bettina Welz
   7. Marc Freichel
   8. Joachim Wittbrodt

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors describe the hei-tag, which, when added to a genome editing enzyme, results in increased editing rates in fish embryos and mammalian cell culture. The hei-tag tool could provide a valuable alternative that can potentially boost genome editing efficiency in different species and systems. The wider applicability of this approach still requires further investigation, since the improvement of editing efficiency is so far supported by experimental data on only a few targets. It would also be important to learn how the authors' design decisions affect activity, especially when benchmarked against current state-of-art genome editing tools.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Epigenetic reprogramming by TET enzymes impacts co-transcriptional R-loops

   This article has 10 authors:

   1. João C Sabino
   2. Madalena R de Almeida
   3. Patrícia L Abreu
   4. Ana M Ferreira
   5. Paulo Caldas
   6. Marco M Domingues
   7. Nuno C Santos
   8. Claus M Azzalin
   9. Ana Rita Grosso
   10. Sérgio Fernandes de Almeida

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The work provides new insight into the potential role of 5hmc DNA in specific transcriptional processes. This implies that 5hmC containing DNA has specific epigenetic features beyond being a simple intermediate in interconversion between repressive 5mC and active C DNA. This work has the merit to focus the attention on a rare DNA modification, helping defining its functions, starting from in vitro evidence and extending these findings in cellular context. There are some weaknesses in the presentation of the data, the controls and the statistical analyses.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Host casein kinase 1-mediated phosphorylation modulates phase separation of a rhabdovirus phosphoprotein and virus infection

   This article has 9 authors:

   1. Xiao-Dong Fang
   2. Qiang Gao
   3. Ying Zang
   4. Ji-Hui Qiao
   5. Dong-Min Gao
   6. Wen-Ya Xu
   7. Ying Wang
   8. Dawei Li
   9. Xian-Bing Wang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Recently liquid-liquid phase separation (LLPS) has emerged as a mechanism by which membraneless compartments are formed inside the cells to compartmentalize biomolecules. In this paper, the authors show that the P protein from a plant-infecting negative sense RNA virus undergoes LLPS to promote virus replication. The host casein kinase 1 phosphorylates P protein and inhibits phase separation and viral replication. This paper will be of interest to virologists and researchers who study LLPS.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

6. Risk of heart disease following treatment for breast cancer – results from a population-based cohort study

   This article has 11 authors:

   1. Haomin Yang
   2. Nirmala Bhoo-Pathy
   3. Judith S Brand
   4. Elham Hedayati
   5. Felix Grassmann
   6. Erwei Zeng
   7. Jonas Bergh
   8. Weiwei Bian
   9. Jonas F Ludvigsson
   10. Per Hall
   11. Kamila Czene

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to oncologists, cardiologists, cardio-oncologists, and primary care providers who treat patients with breast cancer and adds to the growing body of literature that identifies the increased risk for cardiotoxicity associated with breast cancer treatment and does so at the general population level. The results of this study are interesting and supported by the data provided, however they must be interpreted with caution as the database utilized includes intended treatment regimens (chemotherapy, radiotherapy) rather than the confirmed treatments patients received.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

7. Global and context-specific transcriptional consequences of oncogenic Fbw7 mutations

   This article has 11 authors:

   1. H Nayanga Thirimanne
   2. Feinan Wu
   3. Derek H Janssens
   4. Jherek Swanger
   5. Ahmed Diab
   6. Heather M Feldman
   7. Robert A Amezquita
   8. Raphael Gottardo
   9. Patrick J Paddison
   10. Steven Henikoff
   11. Bruce E Clurman

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     FBXW7 is a CRL controlling the abundance of numerous transcription factors through targeted degradation. The authors use isogenic cancer and normal cell lines that differ only in the functional status of FBXW7 to examine the genome-wide effects of FBXW7. The authors demonstrate that null and missense mutations have similar large-scale effects on gene expression and chromatin modification status of many loci at intergenic and intronic regions, but that there are substantial differences in affected loci between the mutant backgrounds. This study represents the first such systematic evaluation of the impact of FBXW7 functional variation on global gene expression.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. Adult mouse fibroblasts retain organ-specific transcriptomic identity

   This article has 14 authors:

   1. Elvira Forte
   2. Mirana Ramialison
   3. Hieu T Nim
   4. Madison Mara
   5. Jacky Y Li
   6. Rachel Cohn
   7. Sandra L Daigle
   8. Sarah Boyd
   9. Edouard G Stanley
   10. Andrew G Elefanty
   11. John Travis Hinson
   12. Mauro W Costa
   13. Nadia A Rosenthal
   14. Milena B Furtado

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of general interest to a broad audience of scientists working in regenerative medicine, tissue engineering, disease modelling, and stem cell fields. It reveals organ fibroblast heterogeneity and shows that the organ-specific identity is preserved in vitro and during ectopic transplantation in vivo. The right choice of fibroblasts might therefore be critical in the fields mentioned above.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Noisy metabolism can promote microbial cross-feeding

   This article has 2 authors:

   1. Jaime G Lopez
   2. Ned S Wingreen

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of broad interest to microbiologists interested in gene expression noise and/or metabolic interactions in microbial communities. It provides a novel hypothesis that complements existing theoretical frameworks. The hypothesis is well supported by data from a mathematical model, and it its predictions could be tested experimentally in future work.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Lead-OR: A multimodal platform for deep brain stimulation surgery

    This article has 10 authors:

    1. Simón Oxenford
    2. Jan Roediger
    3. Clemens Neudorfer
    4. Luka Milosevic
    5. Christopher Güttler
    6. Philipp Spindler
    7. Peter Vajkoczy
    8. Wolf-Julian Neumann
    9. Andrea Kühn
    10. Andreas Horn

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This manuscript is of interest to clinicians and researchers who are involved in both placement and controlling of the accuracy of the location of deep brain stimulation electrodes. The authors present a software tool combining and correlating the documentation of intraoperative neurophysiological findings with atlas and imaging data. They also show an exemplary validation of their tool in a clinical series of 52 Parkinson disease patients who underwent DBS surgery.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife