eLife

Latest preprint reviews

1. No evidence from complementary data sources of a direct glutamatergic projection from the mouse anterior cingulate area to the hippocampal formation

   This article has 7 authors:

   1. Lilya Andrianova
   2. Steliana Yanakieva
   3. Gabriella Margetts-Smith
   4. Shivali Kohli
   5. Erica S Brady
   6. John P Aggleton
   7. Michael T Craig

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this study, the authors attempted to further examine the existence of a potential direct projection from the anterior cingulate cortex to the hippocampus which has important functional implication but is currently supported by only one major publication in the literature. They used distinct anterograde et retrograde viral tracing strategies and analyzed the data available in the Allen atlas but found no evidence in support of the existence of this connection.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

2. Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence

   This article has 8 authors:

   1. Sarah Rose Anderson
   2. Jacqueline M Roberts
   3. Nathaniel Ghena
   4. Emmalyn A Irvin
   5. Joon Schwakopf
   6. Isabelle B Cooperstein
   7. Alejandra Bosco
   8. Monica L Vetter

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work convincingly provides important novel mechanisms on how microglial states are shaped during retinal development, an important question that should be of interest for a broad readership.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Tadr is an axonal histidine transporter required for visual neurotransmission in Drosophila

   This article has 3 authors:

   1. Yongchao Han
   2. Lei Peng
   3. Tao Wang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Han et al. report the discovery of an amino acid transporter that is required locally at axon terminals of fly photoreceptors neurons for the uptake of histidine, the precursor of the neurotransmitter histamine. This function is required for transmitter synthesis locally and neurotransmission. The work exemplifies a specialized model for local monoamine transmitter synthesis at synapses in the nervous system, the generality of which for other monoamine systems remains to be tested.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Neutrophil extracellular traps arm DC vaccination against NPM-mutant myeloproliferation

   This article has 16 authors:

   1. Claudio Tripodo
   2. Barbara Bassani
   3. Elena Jachetti
   4. Valeria Cancila
   5. Claudia Chiodoni
   6. Paola Portararo
   7. Laura Botti
   8. Cesare Valenti
   9. Milena Perrone
   10. Maurilio Ponzoni
   11. Patrizia Comoli
   12. Mara Lecchi
   13. Paolo Verderio
   14. Antonio Curti
   15. Mario P Colombo
   16. Sabina Sangaletti

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors successfully demonstrated the immunogenic effects of a new dendritic cell-based vaccine on AML with NPM1 mutation. Using a transgenic mouse model that carries human NPM1 mutation and an elegant bone marrow chimera approach, they showed that the tumor growth is reduced by the vaccine. Furthermore, they provided evidence for increased CD8+ T cell presence and activity at the tumor site and increased anti-NPMc antibody levels in the serum. These findings are timely and novel and the new methods presented here would be of interest to a broad audience from immunology, inflammation and cancer fields.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. The VINE complex is an endosomal VPS9-domain GEF and SNX-BAR coat

   This article has 5 authors:

   1. Shawn P Shortill
   2. Mia S Frier
   3. Ponthakorn Wongsangaroonsri
   4. Michael Davey
   5. Elizabeth Conibear

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The SNX-BAR family of sorting nexins are a diverse group of dimeric proteins that form tubules from endosomal membranes where they also select protein cargoes for incorporation into these transport carriers. The authors of this manuscript describe a new SNX-BAR complex in budding yeast, named the VINE complex, that uniquely harbors a guanine nucleotide exchange factor (GEF) domain for Rab5-related small GTP-binding proteins. The author's data implicate VINE in endosomal membrane remodeling events and the sorting of a vacuolar hydrolase receptor. The paper would be strengthened by some additional quantifications and by taking advantage of new modeling opportunities.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. Multi-omic rejuvenation of human cells by maturation phase transient reprogramming

   This article has 9 authors:

   1. Diljeet Gill
   2. Aled Parry
   3. Fátima Santos
   4. Hanneke Okkenhaug
   5. Christopher D Todd
   6. Irene Hernando-Herraez
   7. Thomas M Stubbs
   8. Inês Milagre
   9. Wolf Reik

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study describes a novel "maturation phase transient reprogramming" (MPTR) method to restore the epigenome of cells to a more youthful state. The authors demonstrate the effectiveness of the method to reverse several age-related changes including remodeling of the transcriptome. The method appears to peform favorably compared to other transient reprogramming protocols, and the study will be of interest to developmental biologists as well as researchers that study ageing.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Glutathione binding to the plant AtAtm3 transporter and implications for the conformational coupling of ABC transporters

   This article has 2 authors:

   1. Chengcheng Fan
   2. Douglas C Rees

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Oxygen consumption in mitochondria by the respiratory chain leads to a major source of reactive oxygen species, and mitochondrial glutathione is an important line of defence against free radical production. The ABC transporter Atm3 exports oxidized glutathione from the mitochondria to help maintain a suitable reducing environment. Here, the authors have determined structure of Atm3 in multiple conformations by single-particle cryo EM and have revealed new insights into local changes coupled to substrate export of oxidised glutathione and ABC exporters in general. The work will be of interest to the mitochondrial biology and transporter communities.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. Association of egg consumption, metabolic markers, and risk of cardiovascular diseases: A nested case-control study

   This article has 17 authors:

   1. Lang Pan
   2. Lu Chen
   3. Jun Lv
   4. Yuanjie Pang
   5. Yu Guo
   6. Pei Pei
   7. Huaidong Du
   8. Ling Yang
   9. Iona Y Millwood
   10. Robin G Walters
   11. Yiping Chen
   12. Weiwei Gong
   13. Junshi Chen
   14. Canqing Yu
   15. Zhengming Chen
   16. Liming Li
   17. on behalf of China Kadoorie Biobank Collaborative Group

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Pan et al investigated associations of self-reported egg consumption with plasma metabolic markers and these plasma metabolic markers with the risk of cardiovascular diseases. In general there was some impact on metabolic markers which could protect against CVD. The paper will interest scientists in the field of nutritional epidemiology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. SLC38A2 provides proline to fulfill unique synthetic demands arising during osteoblast differentiation and bone formation

   This article has 6 authors:

   1. Leyao Shen
   2. Yilin Yu
   3. Yunji Zhou
   4. Shondra M Pruett-Miller
   5. Guo-Fang Zhang
   6. Courtney M Karner

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The paper unequivocally proves that the key function of proline during bone formation is being incorporated into proline-enriched proteins rather than contributing to other metabolic processes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Humanization of wildlife gut microbiota in urban environments

    This article has 5 authors:

    1. Brian A Dillard
    2. Albert K Chung
    3. Alex R Gunderson
    4. Shane C Campbell-Staton
    5. Andrew H Moeller

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      Urbanization has broad impacts for macroecology but its consequences for wildlife microbial ecology remain unclear. Dillard et al. hypothesize that humans living in an urban setting may transfer their microbes to wildlife with potential adverse effects. They analyze 16S rRNA gene sequencing data from humans, crested anoles, and coyotes, leading to the discovery of multiple bacteria that fit the pattern of urbanization and inter-species transfer.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife