eLife

Latest preprint reviews

1. De novo apical domain formation inside the Drosophila adult midgut epithelium

   This article has 2 authors:

   1. Jia Chen
   2. Daniel St Johnston

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper addresses a fundamental cell biological question - the de-novo development of an apical membrane during the integration of an initially unpolarized cell, the enterocyst, into an an existing epithelium, the Drosophila midgut. The data will be of interest to a wide range of researchers including those in the fields of cell, development, stem cell and cancer biology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. Post-translational modification patterns on β-myosin heavy chain are altered in ischemic and nonischemic human hearts

   This article has 15 authors:

   1. Maicon Landim-Vieira
   2. Matthew C Childers
   3. Amanda L Wacker
   4. Michelle Rodriquez Garcia
   5. Huan He
   6. Rakesh Singh
   7. Elizabeth A Brundage
   8. Jamie R Johnston
   9. Bryan A Whitson
   10. P Bryant Chase
   11. Paul ML Janssen
   12. Michael Regnier
   13. Brandon J Biesiadecki
   14. J Renato Pinto
   15. Michelle S Parvatiyar

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study reports new post-translational modifications (PTMs) to β-myosin heavy chain, using tissue samples from normal and failing human hearts. Atomistic simulations of myosin molecular dynamics suggest that these PTMs lead to meaningful alterations in structure, solvent exposure, and dynamics of certain regions of the protein. These data and simulations provide a foundation for further work to determine the precise functional significance of β-myosin heavy chain PTMs. The work will be of interest to cell biologists and cardiologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

3. Development and evaluation of a machine learning-based in-hospital COVID-19 disease outcome predictor (CODOP): A multicontinental retrospective study

   This article has 28 authors:

   1. Riku Klén
   2. Disha Purohit
   3. Ricardo Gómez-Huelgas
   4. José Manuel Casas-Rojo
   5. Juan Miguel Antón-Santos
   6. Jesús Millán Núñez-Cortés
   7. Carlos Lumbreras
   8. José Manuel Ramos-Rincón
   9. Noelia García Barrio
   10. Miguel Pedrera-Jiménez
   11. Antonio Lalueza Blanco
   12. María Dolores Martin-Escalante
   13. Francisco Rivas-Ruiz
   14. Maria Ángeles Onieva-García
   15. Pablo Young
   16. Juan Ignacio Ramirez
   17. Estela Edith Titto Omonte
   18. Rosmery Gross Artega
   19. Magdy Teresa Canales Beltrán
   20. Pascual Ruben Valdez
   21. Florencia Pugliese
   22. Rosa Castagna
   23. Ivan A Huespe
   24. Bruno Boietti
   25. Javier A Pollan
   26. Nico Funke
   27. Benjamin Leiding
   28. David Gómez-Varela

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This article is dealing with the unmet need to generate a machine-learning approach for the early and accurate estimation of the risk among COVID-19 admission. The presented data generate confidence on the validity since they have been developed in a vast number of patients and they are validated in cohorts from different geographical regions.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

4. Genetic variation of putative myokine signaling is dominated by biological sex and sex hormones

   This article has 7 authors:

   1. Leandro M Velez
   2. Cassandra Van
   3. Timothy Moore
   4. Zhenqi Zhou
   5. Casey Johnson
   6. Andrea L Hevener
   7. Marcus M Seldin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This elegantly performed systems-genetics paper on the predicted human skeletal muscle secretome highlights the importance of sex and sex hormones in regulating myokine expression and predicted cross-tissue effects. Male and female mice lacking estrogen receptor α (Esr1) were used to understand how estrogen signalling affects myokine genes expression. The methods used and data presented in this manuscript can serve as an important resource for other researchers in the field.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Visualizing molecules of functional human profilin

   This article has 6 authors:

   1. Morgan L Pimm
   2. Xinbei Liu
   3. Farzana Tuli
   4. Jennifer Heritz
   5. Ashley Lojko
   6. Jessica L Henty-Ridilla

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to a broad audience of cell biologists and biochemists who study the cytoskeleton. It reports the development and rigorous characterization of a fully functional, fluorescently labeled version of profilin that can be used to visualize profilin's dynamic interactions in live cells. Owing to profilin's dual functions in regulating actin and microtubule assembly, this technological development will be a useful tool for a wide range of studies aimed at understanding the role of the cytoskeleton in driving fundamental cellular processes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife, ASAPbio crowd review

6. A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration

   This article has 7 authors:

   1. Ana S Brandão
   2. Jorge Borbinha
   3. Telmo Pereira
   4. Patrícia H Brito
   5. Raquel Lourenço
   6. Anabela Bensimon-Brito
   7. Antonio Jacinto

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors provide compelling evidence to show that injury induces activation of glycolysis during zebrafish adult tail fin regeneration. This early activation is crucial for osteoblast dedifferentiation and proliferation, which are required for blastema formation and tail fin regeneration. However, additional data are required to support the claim of a "metabolic switch" from oxidative phosphorylation to glycolysis. This study will be of interest to a broad audience in the fields of regeneration and metabolic regulation of developmental processes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. A dynamic clamp protocol to artificially modify cell capacitance

   This article has 6 authors:

   1. Paul Pfeiffer
   2. Federico José Barreda Tomás
   3. Jiameng Wu
   4. Jan-Hendrik Schleimer
   5. Imre Vida
   6. Susanne Schreiber

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript introduces a new enhancement to the dynamic clamp technique, CapClamp that, analogous to the artificial conductances of standard Dynamic Clamp, allows the experimenter to adjust the somatic time constant by setting a new membrane artificial capacitance independent of any change in input resistance. The technique is shown to have application for studying temporal integration, energetic costs of spiking and bifurcations. The technique is rigorously tested in model and physiological application and is robust when sampling frequency of the feedback (clamp) loop is fast compared to the fastest electrical event in a neuron (usually action potentials), and for vertebrate neurons it should be 20KHz or faster and yet faster for fast spiking neurons.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

8. Putting the theory into ‘burstlet theory’ with a biophysical model of burstlets and bursts in the respiratory preBötzinger complex

   This article has 2 authors:

   1. Ryan S Phillips
   2. Jonathan E Rubin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript is of significant interest to readers in the field of neural control of breathing and for researches interested in the generation of biological rhythms in general. The study assembles a sophisticated computational modelling approach to test long-standing theories and emerging views in neural control of breathing and more specifically on biophysical mechanisms of burstlet generation in the respiratory network (the preBötzinger complex network). This work is an important contribution to a better understanding of the respiratory rhythm generation, will help validate (or not) running hypotheses and will guide future experiments.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

9. Molecular determinants of complexin clamping and activation function

   This article has 5 authors:

   1. Manindra Bera
   2. Sathish Ramakrishnan
   3. Jeff Coleman
   4. Shyam S Krishnakumar
   5. James E Rothman

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Berra and colleagues revisit several mechanistic questions mainly centered on the accessory helix of mouse complexin (mCpx) and its contribution to the 'fusion clamp' property of mCpx whereby mCpx-SNARE interactions prevent full assembly and subsequent membrane fusion. This clamping function is believed to help generate a metastable pool of release-ready vesicles at the synapse, and it has been studied in a wide variety of systems including mouse, fly, worm, squid, fish, and diverse in vitro biochemical preps over the past ~ 20 years. The authors derive several conclusions from their efforts, but most relevant is a reiteration of a previous proposal that the accessory helix region of mCpx stabilizes a pre-fusion clamped state via interactions with SNAREs.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Association analyses of host genetics, root-colonizing microbes, and plant phenotypes under different nitrogen conditions in maize

    This article has 11 authors:

    1. Michael A Meier
    2. Gen Xu
    3. Martha G Lopez-Guerrero
    4. Guangyong Li
    5. Christine Smith
    6. Brandi Sigmon
    7. Joshua R Herr
    8. James R Alfano
    9. Yufeng Ge
    10. James C Schnable
    11. Jinliang Yang

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The authors have produced a rich dataset that illuminates how root-associated bacteria differ among diverse maize lines, under low and high nitrogen treatment. The authors make use of this dataset to describe interesting patterns related to the genetic architecture of factors associated with maize rhizosphere microbiome assembly, although it is not clear yet whether the microbiome is an agent of natural selection in this case, or whether other selective forces shape maize roots in a manner that in turn affect smicrobiome recruitment. In addition to interesting insights reported in the present manuscript, these data are likely to be used for and/or compared to, in many future studies.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife