eLife

Latest preprint reviews

1. A neurogenic signature involving monoamine Oxidase-A controls human thermogenic adipose tissue development

   This article has 13 authors:

   1. Javier Solivan-Rivera
   2. Zinger Yang Loureiro
   3. Tiffany DeSouza
   4. Anand Desai
   5. Sabine Pallat
   6. Qin Yang
   7. Raziel Rojas-Rodriguez
   8. Rachel Ziegler
   9. Pantos Skritakis
   10. Shannon Joyce
   11. Denise Zhong
   12. Tammy Nguyen
   13. Silvia Corvera

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript uses a species-hybrid model in which functional human white and thermogenic adipose tissues develop in mice. Interestingly, human adipose tissue is fully able to recruit mouse vascular networks and sympathetic innervation during human adipocyte development. These findings provide novel and valuable information about the development of human thermogenic adipose tissue.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. A CRISPR screen in intestinal epithelial cells identifies novel factors for polarity and apical transport

   This article has 8 authors:

   1. Katharina MC Klee
   2. Michael W Hess
   3. Michael Lohmüller
   4. Sebastian Herzog
   5. Kristian Pfaller
   6. Thomas Müller
   7. Georg F Vogel
   8. Lukas A Huber

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Here, the authors performed a CRISPR/Cas9 loss of function screen in polarized human epithelial cells to identify novel regulators of epithelial polarization and polarized membrane trafficking. This study provides a powerful resource for future investigations to unravel the complexity and diversity of mechanisms underlying epithelial polarization and polarized cargo transport. Furthermore, this dataset may represent an essential contribution to investigating novel congenital diseases associated with these processes, e.g., microvillus inclusion disease and necrotizing enterocolitis. A few experiments are suggested to bolster the authors' conclusions regarding the roles of key hits.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

3. Consistent coordination patterns provide near perfect behavior decoding in a comprehensive motor program for insect flight

   This article has 6 authors:

   1. Joy Putney
   2. Marko Angjelichinoski
   3. Robert Ravier
   4. Silvia Ferrari
   5. Vahid Tarokh
   6. Simon Sponberg

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript bridges neurophysiology and biomechanics and is of broad interest in improving our understanding of insect flight control. Here, Putney et al. record the activity of the flight muscles of tethered hawkmoths and demonstrate that the direction of the visual stimulus to which the insect responds can be classified using precisely timed information on muscle activity from a subset of the flight muscles. This is an important step in identifying the mapping from visual input to motor output, albeit that the mapping identified here is qualitative (i.e. classification of visual stimulus direction) rather than quantitative (i.e. prediction of output torque or apparent angular velocity of self-motion).

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

4. Effectiveness of rapid SARS-CoV-2 genome sequencing in supporting infection control for hospital-onset COVID-19 infection: Multicentre, prospective study

   This article has 51 authors:

   1. Oliver Stirrup
   2. James Blackstone
   3. Fiona Mapp
   4. Alyson MacNeil
   5. Monica Panca
   6. Alison Holmes
   7. Nicholas Machin
   8. Gee Yen Shin
   9. Tabitha Mahungu
   10. Kordo Saeed
   11. Tranprit Saluja
   12. Yusri Taha
   13. Nikunj Mahida
   14. Cassie Pope
   15. Anu Chawla
   16. Maria-Teresa Cutino-Moguel
   17. Asif Tamuri
   18. Rachel Williams
   19. Alistair Darby
   20. David L Robertson
   21. Flavia Flaviani
   22. Eleni Nastouli
   23. Samuel Robson
   24. Darren Smith
   25. Matthew Loose
   26. Kenneth Laing
   27. Irene Monahan
   28. Beatrix Kele
   29. Sam Haldenby
   30. Ryan George
   31. Matthew Bashton
   32. Adam A Witney
   33. Matthew Byott
   34. Francesc Coll
   35. Michael Chapman
   36. Sharon J Peacock
   37. COG-UK HOCI Investigators
   38. The COVID-19 Genomics UK (COG-UK) consortium
   39. Joseph Hughes
   40. Gaia Nebbia
   41. David G Partridge
   42. Matthew Parker
   43. James Richard Price
   44. Christine Peters
   45. Sunando Roy
   46. Luke B Snell
   47. Thushan I de Silva
   48. Emma Thomson
   49. Paul Flowers
   50. Andrew Copas
   51. Judith Breuer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a very extensive evaluation of the impact of rapid availability of whole-gemome sequencing results from SARS-CoV2 to inform infection control policies in hospital settings. It, most likely, is the largest analysis of its kind, clearly demonstrating the possibilities and challenges with this innovative technique.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

5. Both prey and predator features predict the individual predation risk and survival of schooling prey

   This article has 7 authors:

   1. Jolle Wolter Jolles
   2. Matthew MG Sosna
   3. Geoffrey PF Mazué
   4. Colin R Twomey
   5. Joseph Bak-Coleman
   6. Daniel I Rubenstein
   7. Iain D Couzin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study, which will be of interest to behavioral ecologists, uses highly quantitative video tracking approaches to understand the predictors of predators' success in attacking schooling fish and will be of interest to behavioral, evolutionary, and movement ecologists. While some of the results seem unsurprising (e.g., that predators tend to successfully capture prey that are closer to them), the manuscript as a whole highlights the importance of tracking the perspective of the predator as well as of the prey, and shows that animals that are central to a group may sometimes be the most vulnerable. Although the experiments and data analyses are commendable, the manuscript would benefit from more careful discussion of its overall implications for the evolution of collective behavior, including potential limits of the experimental design.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Mapping the single-cell landscape of acral melanoma and analysis of the molecular regulatory network of the tumor microenvironments

   This article has 13 authors:

   1. Zan He
   2. Zijuan Xin
   3. Qiong Yang
   4. Chen Wang
   5. Meng Li
   6. Wei Rao
   7. Zhimin Du
   8. Jia Bai
   9. Zixuan Guo
   10. Xiuyan Ruan
   11. Zhaojun Zhang
   12. Xiangdong Fang
   13. Hua Zhao

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     He and collaborators analyse eight samples from six patients with acral melanoma through single-cell RNA sequencing. They describe the tumour microenvironment in these tumours, including descriptions of interactions among distinct cell types and potential biomarkers. The study is thoroughly done. In its final form, this study will help to inform our knowledge of the immune infiltration on the poorly studied acral melanoma subtype, the most common type of the disease in several countries in Africa, Asia and Latin America.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

7. COVID-19 pandemic dynamics in South Africa and epidemiological characteristics of three variants of concern (Beta, Delta, and Omicron)

   This article has 2 authors:

   1. Wan Yang
   2. Jeffrey L Shaman

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper presents a modeling framework that can be used to track the complex behavioral and immunological landscape of the COVID-19 pandemic over multiple surges and variants in South Africa, which has been validated previously for other regions and time periods. This work may be useful for infectious disease modelers, epidemiologists, and public health officials as they navigate the next phase of the pandemic or seek to understand the history of the epidemic in South Africa.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

8. Neuroinflammation in neuronopathic Gaucher disease: Role of microglia and NK cells, biomarkers, and response to substrate reduction therapy

   This article has 17 authors:

   1. Chandra Sekhar Boddupalli
   2. Shiny Nair
   3. Glenn Belinsky
   4. Joseph Gans
   5. Erin Teeple
   6. Tri-Hung Nguyen
   7. Sameet Mehta
   8. Lilu Guo
   9. Martin L Kramer
   10. Jiapeng Ruan
   11. Honggge Wang
   12. Matthew Davison
   13. Dinesh Kumar
   14. DJ Vidyadhara
   15. Bailin Zhang
   16. Katherine Klinger
   17. Pramod K Mistry

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Patients with Gaucher disease can have significant and crippling neurological manifestations. The study uses novel mouse models of neurodegeneration associated with glucocerebrosidase 1 (GBA1) deficiency. It provides a detailed analysis of the alterations caused by targeted deletion of GBA1, including cellular, genetic and metabolic alterations in neurons, microglia, and infiltrating immune cells. The work defines novel mechanisms driving neuroinflammation in neuronopathic Gaucher disease.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. 3D optogenetic control of arteriole diameter in vivo

   This article has 5 authors:

   1. Philip J O'Herron
   2. David A Hartmann
   3. Kun Xie
   4. Prakash Kara
   5. Andy Y Shih

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript by O'Herron et al. describes an all-optical method combining optogenetic stimulation and 2-photon microscopy imaging to simultaneously manipulate and monitor brain microvasculature contractility in three dimensions. The method employs a spatial light modulator to create three-dimensional activation patterns in the brains of cranial window-model transgenic mice expressing the excitatory opsin, ReaChR, in mural cells (smooth muscle cells and pericytes). This provides a powerful new in vivo technique to control blood flow into the brain and to understand its actions on brain function.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

10. In situ X-ray-assisted electron microscopy staining for large biological samples

    This article has 5 authors:

    1. Sebastian Ströh
    2. Eric W Hammerschmith
    3. David W Tank
    4. H Sebastian Seung
    5. Adrian Andreas Wanner

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study explores the kinetics of heavy metal staining of tissue using time-lapse imaging with X-ray micro computed tomography (CT). It will be of interest to the wide community of scientists preparing biological samples for electron microscopy (EM), in particular large-volume EM. While at present the relation between CT imaging and EM contrast remains to be quantified, this study has the potential to become a reference for the field in establishing a quantitative tool for assessing and developing staining protocols.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife