eLife

Latest preprint reviews

1. p75NTR prevents the onset of cerebellar granule cell migration via RhoA activation

   This article has 2 authors:

   1. Juan P Zanin
   2. Wilma J Friedman

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to neurobiologists and developmental biologists. Identifying novel mechanisms that prevent excessive neuronal migration is an important contribution to the field of neural development. The key conclusions of the paper are well supported by the data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. Specific binding of Hsp27 and phosphorylated Tau mitigates abnormal Tau aggregation-induced pathology

   This article has 15 authors:

   1. Shengnan Zhang
   2. Yi Zhu
   3. Jinxia Lu
   4. Zhenying Liu
   5. Amanda G Lobato
   6. Wen Zeng
   7. Jiaqi Liu
   8. Jiali Qiang
   9. Shuyi Zeng
   10. Yaoyang Zhang
   11. Cong Liu
   12. Jun Liu
   13. Zhuohao He
   14. R Grace Zhai
   15. Dan Li

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors found elevated Hsp 27 levels in brains from Alzheimer disease patients. Hsp27 co-localized with p-Tau, efficiently prevented pTau fibrillation in vitro, and mitigated neuropathology of pTau aggregation in a Drosophila tauopathy model. A series of biochemical assays is presented to supporrt the claim that Hsp27 prevents abnormal Tau aggregation and p-Tau pathology. Overall, the study is well designed and presented, and the data convincingly support this major conclusion, which is relevant to colleagues studying neurodegeneration.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Live-cell imaging in human colonic monolayers reveals ERK waves limit the stem cell compartment to maintain epithelial homeostasis

   This article has 11 authors:

   1. Kelvin W Pond
   2. Julia M Morris
   3. Olga Alkhimenok
   4. Reeba P Varghese
   5. Carly R Cabel
   6. Nathan A Ellis
   7. Jayati Chakrabarti
   8. Yana Zavros
   9. Juanita L Merchant
   10. Curtis A Thorne
   11. Andrew L Paek

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Pond et al. established a 2D patient-derived organoid screening platform to study tissue patterning and kinase pathway dynamics. They aim to understand how the spacing of different colonic cell types and their communication are regulated. They found that apoptosis induces Erk signaling waves that prime cell movement and are essential to maintain tissue patterning in the organoid monolayers. The work presented here is of importance to the field and provides insights into how Erk waves driven by apoptosis can help maintain gut homeostasis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. DetecDiv, a generalist deep-learning platform for automated cell division tracking and survival analysis

   This article has 3 authors:

   1. Théo Aspert
   2. Didier Hentsch
   3. Gilles Charvin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This article introduces "DetecDiv", a high throughput, deep learning method to perform automated cell-division tracking in yeast. The performance of the method, estimated to be 100 times faster than manual annotation, overcomes current time processing limitations that are inherent to large single cell datasets. In particular, DetecDiv allows to automatically get quantitative measurements of replicative life span in yeast. The method is of broad interest for quantitative biology as it can be used to study yeast cells dynamics across their lifespan.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior

   This article has 8 authors:

   1. Ian G McLachlan
   2. Talya S Kramer
   3. Malvika Dua
   4. Elizabeth M DiLoreto
   5. Matthew A Gomes
   6. Ugur Dag
   7. Jagan Srinivasan
   8. Steven W Flavell

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study asks how diverse signals are integrated at the cellular level to generate adaptive behaviors. The authors show that prolonged food deprivation (i.e. fasting) of C. elegans broadly alters gene expression in food sensing neurons, thereby altering foraging behavior and chemosensory neuron responses to food. The fasting-induced genes include many chemoreceptors, one of which mediates responses to specific volatile components of food. Finally, they show that food controls the expression of a fasting-induced chemoreceptor via multiple external (i.e. sensory) and internal (potentially metabolic) cues. The paper is of importance to scientists with an interest in adaptive behaviour as well as the cellular and molecular mechanisms underlying integration of stimuli.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

6. “Integration of multimodal data in the developing tooth reveals candidate dental disease genes”

   This article has 3 authors:

   1. Emma Wentworth Winchester
   2. Alexis Hardy
   3. Justin Cotney

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study, which will be of interest to basic scientists and clinicians in the fields of dental and craniofacial malformations, explores the validity and usefulness of mouse teeth as a model for studying the role of enhancers in human dental development and disease, based on a multi-layered integrated analysis of genetic data with different available data from mice and humans. The results from the study can provide a useful tool for the manipulation of expression of reporter or other genes in a tooth- or enamel-specific manner.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

7. CriSNPr, a single interface for the curated and de novo design of gRNAs for CRISPR diagnostics using diverse Cas systems

   This article has 5 authors:

   1. Asgar H Ansari
   2. Manoj Kumar
   3. Sajal Sarkar
   4. Souvik Maiti
   5. Debojyoti Chakraborty

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The web-based software developed in this study will be of interest to researchers who develop CRISPR-based diagnostic methods. The use of CRISPR-Cas to rapidly identify specific mutations in both cancer and infection is an evolving field with good potential to play a role in future research and diagnostics. This software will facilitate the implementation of such technologies and is therefore useful.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. A high-throughput yeast display approach to profile pathogen proteomes for MHC-II binding

   This article has 4 authors:

   1. Brooke D Huisman
   2. Zheng Dai
   3. David K Gifford
   4. Michael E Birnbaum

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript builds on previous work to design yeast display libraries representing full viral proteomes with overlapping 15-mer peptides binding to specific HLA-DR alleles, and therefore potentially immunogenic for CD4 T cell responses. The authors use SARS-CoV-2 and dengue viruses as proof of concept and identify a number of potentially immunogenic peptides not predicted by current algorithms. The methods are interesting and promising and will be of interest to a wide range of researchers in immunological and infectious disease studies.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

9. Using adopted individuals to partition indirect maternal genetic effects into prenatal and postnatal effects on offspring phenotypes

   This article has 3 authors:

   1. Liang-Dar Hwang
   2. Gunn-Helen Moen
   3. David M Evans

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to scientists interested in intergenerational transmission of phenotypes through genetic pathways. The authors propose an innovative and sound method to leverage the adoption of a design for disentangling prenatal and postnatal genetic effects. Additional analyses are needed to address the limitations of the model applied to the specific dataset that was used to illustrate the method.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Dynamic allostery in substrate binding by human thymidylate synthase

    This article has 3 authors:

    1. Jeffrey P Bonin
    2. Paul J Sapienza
    3. Andrew L Lee

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The authors analyze the mechanisms of entropically driven cooperativity in the human thymidylate synthase (hTS), an enzyme essential for DNA replication and a promising target for anticancer drugs. The authors conclude that the cooperative binding of dUMP ligands to its two identical sites arises from a disproportionate reduction in the enzyme's conformational entropy upon binding the first ligand. The results provide rare insights into the mechanisms of ligand binding for an essential human protein and should be of great interest to readers interested in enzyme structure/dynamics/function relationships, cooperativity and allostery, and possible drug targeting of thymidylate synthase.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)”

    Reviewed by eLife