eLife

Latest preprint reviews

1. Gas6 drives Zika virus-induced neurological complications in humans and congenital syndrome in immunocompetent mice

   This article has 46 authors:

   1. Joao Luiz Silva-Filho
   2. Lilian G. de Oliveira
   3. Leticia Monteiro
   4. Pierina L. Parise
   5. Nagela G. Zanluqui
   6. Carolina M. Polonio
   7. Carla L. de Freitas
   8. Daniel A. Toledo-Teixeira
   9. William M. de Souza
   10. Najara Bittencourt
   11. Mariene R. Amorim
   12. Julia Forato
   13. Stéfanie P. Muraro
   14. Gabriela F. de Souza
   15. Matheus C. Martini
   16. Karina Bispo-dos-Santos
   17. Aline Vieira
   18. Carla C. Judice
   19. Glaucia M. Pastore
   20. Eliana Amaral
   21. Renato Passini Junior
   22. Helaine M.B.P. Mayer-Milanez
   23. Carolina C. Ribeiro-do-Valle
   24. Roseli Calil
   25. João Renato Bennini Junior
   26. Giuliane J. Lajos
   27. Albina Altemani
   28. Marcos T. Nolasco da Silva
   29. Ana Carolina Coan
   30. Maria Francisca Colella-Santos
   31. Andrea P.B. von Zuben
   32. Marco Aurélio R. Vinolo
   33. Clarice Weis Arns
   34. Rodrigo Ramos Catharino
   35. Maria Laura Costa
   36. Rodrigo N. Angerami
   37. André R.R. Freitas
   38. Mariangela R. Resende
   39. Márcia T. Garcia
   40. Maria Luiza Moretti
   41. Laurent Renia
   42. Lisa F.P. Ng
   43. Carla V. Rothlin
   44. Fabio T.M. Costa
   45. Jean Pierre Schatzmann Peron
   46. José Luiz Proença-Modena

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The pathophysiology of Zika virus infection remains an area of high research interest. In this study, the authors use diverse experimental models to examine the potential role of Gas6 in Zika virus infection and associated neurological sequelae.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. Intrinsic excitability mechanisms of neuronal ensemble formation

   This article has 4 authors:

   1. Tzitzitlini Alejandre-García
   2. Samuel Kim
   3. Jesús Pérez-Ortega
   4. Rafael Yuste

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript reveals the contribution of intrinsic excitability to the formation of cortical neuronal ensembles. By combining optogenetic and electrophysiological approaches in vitro, the authors provide new insight regarding the role that plasticity of membrane excitability (intrinsic plasticity) plays in synaptic plasticity and the formation of memories.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Transient exposure of a buried phosphorylation site in an autoinhibited protein

   This article has 3 authors:

   1. Simone Orioli
   2. Carl G. Henning Hansen
   3. Kresten Lindorff-Larsen

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript describes a metadynamics simulation-based characterization of the binding and unbinding dynamics of a buried phosphorylated residue in the inhibitory module to the functional domain of Vav1, in an effort to shed lights on the autoinhibition mechanism. The work led to a conformation-selection scenario of the event. The authors discussed the inconsistency between the computational findings and the NMR data, in terms of the free energy differences between the bound and unbound states. Further justification is required for the somewhat ad hoc choice of three collective variables for the metadynamics simulations, including two that are highly correlated.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Comparative transcriptome analysis of human and murine choroidal neovascularization identifies fibroblast growth factor inducible-14 as phylogenetically conserved mediator of neovascular age-related macular degeneration

   This article has 8 authors:

   1. Julian Wolf
   2. Anja Schlecht
   3. Dennis-Dominik Rosmus
   4. Stefaniya Boneva
   5. Hansjürgen Agostini
   6. Günther Schlunck
   7. Peter Wieghofer
   8. Clemens Lange

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors present here an analysis of the gene expression patterns of lesions as found in patients wet age-related macular degeneration, known as choroidal neovascularization. This gene expression analysis is compared to an experimental mouse model. Using this analysis, the authors have identified a candidate target, FN14, as a potential target for the treatment of choroidal neovascularization. Identifying an alternative treatment modality is important for the treatment of these lesions beyond the current standard of care.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. A neural mechanism for detecting object motion during self-motion

   This article has 3 authors:

   1. HyungGoo R Kim
   2. Dora E Angelaki
   3. Gregory C DeAngelis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of broad interest to readers in the field of motion perception. The authors use concurrent psychophysics and single unit recordings, along with modeling, to investigate how primate cortical area MT uses specific visual signals to make inferences that distinguish between visual motion induced by self-motion and the motion of other objects in the world. The experiments and stimuli are expertly designed and the analyses are careful.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

6. Directing Cholangiocyte Morphogenesis in Natural Biomaterial Scaffolds

   This article has 6 authors:

   1. Quinton Smith
   2. Jennifer Bays
   3. Linqing Li
   4. Haniyah Shareef
   5. Christopher S. Chen
   6. Sangeeta N. Bhatia

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest for cell biologists, developmental biologists and tissue engineers. The authors identify a combination of natural extracellular matrix and growth factors that enables to grow cholangiocytes as branched three-dimensional ducts in culture. The work is physiologically relevant and represents an interesting step forward in the study of bile duct formation and disease, although the cultured ducts could be characterized more in depth.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Subcellular Dynamic Immunopatterning of Cytosolic Protein Complexes on Microstructured Polymer Substrates

   This article has 5 authors:

   1. Roland Hager
   2. Ulrike Müller
   3. Nicole Ollinger
   4. Julian Weghuber
   5. Peter Lanzerstorfer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes a method of evaluating the steady state levels and kinetics of protein-protein interactions at the plasma membrane of living cells. This approach builds on previous work in this area, and with stronger validation and demonstration of biological applications, it may be complementary to other biochemical or imaging-based approaches to address important questions related to mechanisms of cell signaling.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12 –tubulin complex

   This article has 14 authors:

   1. Shinya Taguchi
   2. Juri Nakano
   3. Tsuyoshi Imasaki
   4. Tomoki Kita
   5. Yumiko Saijo-Hamano
   6. Naoki Sakai
   7. Hideki Shigematsu
   8. Hiromichi Okuma
   9. Takahiro Shimizu
   10. Eriko Nitta
   11. Satoshi Kikkawa
   12. Satoshi Mizobuchi
   13. Shinsuke Niwa
   14. Ryo Nitta

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Here, Taguchi et al. study a member of the kinesin-4 family of motors, which is important in controlling microtubule length during normal development and maintenance. The authors aim to determine how a member of the kinesin-4 family is able to stabilize the tips of microtubules to suppress both their growth and shrinkage. This paper provides compelling data on KLP-12 by combining in vivo C. elegans work with in vitro single-molecule analysis and structural studies of the motor domain. The structure shows that KLP-12 bends tubulin heterodimers to a level that lies in between the extremes of bending by KIF5B (lattice stabilizer) and KIF2C (lattice destabilizer). This study will be of interest to those in the fields of neuronal development and cytoskeletal dynamics.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

9. Photoreceptors generate neuronal diversity in their target field through a Hedgehog morphogen gradient in Drosophila

   This article has 4 authors:

   1. Matthew P Bostock
   2. Anadika R Prasad
   3. Alicia Donoghue
   4. Vilaiwan M Fernandes

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this paper, Bostock and Fernandes show that photoreceptor axons provide a graded Hedgehog signal to their target region, the lamina, that is highest in the distal and lowest in the proximal lamina. High levels of Hh favor specification of the distally located lamina neurons L2 and L3, while low levels favor specification of the proximal L5 neurons. This graded response raises interesting parallels with the patterning of the vertebrate spinal cord. The paper will be of special interest to those who study optic lobe development, but will also be of more general interest to developmental neurobiology.

     This manuscript was co-submitted with: https://www.biorxiv.org/content/10.1101/2021.12.13.472383v2

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. ATP binding facilitates target search of SWR1 chromatin remodeler by promoting one-dimensional diffusion on DNA

    This article has 10 authors:

    1. Claudia C Carcamo
    2. Matthew F Poyton
    3. Anand Ranjan
    4. Giho Park
    5. Robert K Louder
    6. Xinyu A Feng
    7. Jee Min Kim
    8. Thuc Dzu
    9. Carl Wu
    10. Taekjip Ha

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      Using biophysical measurements, this work establishes that the SWR1 chromatin remodeling complex, which directs incorporation of the histone variant H2A.Z adjacent to nucleosome depleted regions, preferentially associates with longer DNA fragments. It is proposed that larger stretches of free DNA determine the specificity of the complex in vivo. These findings will be of general interest to researchers interested in understanding how chromatin remodelling enzymes act to influence the localisation of histone variants.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

    Reviewed by eLife