eLife

Latest preprint reviews

1. Allosteric stabilization of calcium and phosphoinositide dual binding engages several synaptotagmins in fast exocytosis

   This article has 5 authors:

   1. Janus RL Kobbersmed
   2. Manon MM Berns
   3. Susanne Ditlevsen
   4. Jakob B Sørensen
   5. Alexander M Walter

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Synaptotagmins are Ca2+ sensors for synchronous neurotransmitter release. However, despite intense study it remains unclear exactly how the binding of 5 Ca2+ ions to Synaptotagmin's two C2 domains leads to the observed Ca2+ dependence of vesicle fusion. This study puts forward a novel mechanistic model of neurotransmitter vesicle fusion (vesicle exocytosis) which is relatively simple but significantly more detailed than the widely used phenomenological models of calcium-dependent fast exocytosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. State-dependent activity dynamics of hypothalamic stress effector neurons

   This article has 7 authors:

   1. Aoi Ichiyama
   2. Samuel Mestern
   3. Gabriel B Benigno
   4. Kaela E Scott
   5. Brian L Allman
   6. Lyle Muller
   7. Wataru Inoue

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The observations from high quality in vivo and in vitro recordings of tagged CHR neurons are supported by a computational model that suggests feedback inhibition may regulate the activity patterns of CRH neurons in distinct states, and represent an important contribution. The authors also present an unexpected observation that uncovers interesting neural dynamics that will provide the impetus for new studies exploring firing characteristics in discrete physiological and emotional states. Previous work reaching an opposite conclusion and likely effects of urethane on in vivo recordings are not discussed.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

3. Widespread multi-targeted therapy resistance via drug-induced secretome fucosylation

   This article has 20 authors:

   1. Mark Borris D. Aldonza
   2. Junghwa Cha
   3. Insung Yong
   4. Jayoung Ku
   5. Dabin Lee
   6. Pavel Sinitcyn
   7. Ryeong-Eun Cho
   8. Roben D. Delos Reyes
   9. Dongwook Kim
   10. Hye-Jin Sung
   11. Soyeon Kim
   12. Minjeong Kang
   13. Yongsuk Ku
   14. Geonho Park
   15. Han Suk Ryu
   16. Sukki Cho
   17. Tae Min Kim
   18. Pilnam Kim
   19. Je-Yoel Cho
   20. Yoosik Kim

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript provides a comprehensive unbiased analysis of the fucosylation secretome and correlates with drug response in cancer. It uses a combination of bioinformatic based analyses of multiple datasets and cell based data to identify changes in the secretome and correlates this to drug responses to several targeted therapies.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

4. Ancestral reconstruction of duplicated signaling proteins reveals the evolution of signaling specificity

   This article has 2 authors:

   1. Isabel Nocedal
   2. Michael T Laub

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript is of interest to protein biochemists, protein engineers, and those interested in molecular evolution. The computation and experiments presented in this paper are very logical and rigorously performed. The results provide an example of how protein interaction specificity can be rewired using a small number of mutations, in the context of ancestral sequence reconstruction.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

5. Native American genetic ancestry and pigmentation allele contributions to skin color in a Caribbean population

   This article has 12 authors:

   1. Khai C Ang
   2. Victor A Canfield
   3. Tiffany C Foster
   4. Thaddeus D Harbaugh
   5. Kathryn A Early
   6. Rachel L Harter
   7. Katherine P Reid
   8. Shou Ling Leong
   9. Yuka Kawasawa
   10. Dajiang Liu
   11. John W Hawley
   12. Keith C Cheng

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The work reports collection of DNA from 400-plus individuals from the Kalinago territory of Dominica, and shows their relationships with other ancestries, along with a more specific genetic analysis of skin pigmentation. The material is clearly rare and hard to obtain and the analyses are extremely interesting. The work will be of interest to all studying human populations.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Batch Production of High-Quality Graphene Grids for Cryo-EM: Cryo-EM Structure of Methylococcus capsulatus Soluble Methane Monooxygenase Hydroxylase

   This article has 8 authors:

   1. Eungjin Ahn
   2. Byungchul Kim
   3. Soyoung Park
   4. Amanda L. Erwin
   5. Suk Hyun Sung
   6. Robert Hovden
   7. Shyamal Mosalaganti
   8. Uhn-Soo Cho

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this work, the authors describe a protocol for coating supports for transmission electron microscopy with graphene. The approach uses a commercially available PMMA/graphene pad and the authors provide a new process for PMMA removal after graphene transfer. This work has the potential to be an important methodological step forward - if a way were found to remove amorphous residue from the surface of graphene. The work has the potential to be of broad interest to the many structural biologists using cryo-EM techniques.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Locally adaptive temperature response of vegetative growth in Arabidopsis thaliana

   This article has 8 authors:

   1. Pieter Clauw
   2. Envel Kerdaffrec
   3. Joanna Gunis
   4. Ilka Reichardt-Gomez
   5. Viktoria Nizhynska
   6. Stefanie Koemeda
   7. Jakub Jez
   8. Magnus Nordborg

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work investigates natural variation in plant growth plasticity in Arabidopsis thaliana. Notably, the paper shows that plants from cold regions show less growth response to temperature than plants from warmer regions and this variation in response is consistent with local adaptation. These results have clear relevance for those specialized in working on A. thaliana life history.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses

   This article has 6 authors:

   1. Ivonne Sehring
   2. Hossein Falah Mohammadi
   3. Melanie Haffner-Luntzer
   4. Anita Ignatius
   5. Markus Huber-Lang
   6. Gilbert Weidinger

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work is of interest for readers in the field of bone regeneration, and more broadly to readers in the field of tissue repair and regenerative medicine. The authors took advantage of a well-established in vivo model, live imaging, pharmacological inhibition and genetic strategies to dissect the interrelations of key cellular events in zebrafish fin regeneration. The finding of how distinct generic injury responses are differentially regulated, and are functioning independently from each other, is a valuable piece of information for the community.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. Understanding drivers of phylogenetic clustering and terminal branch lengths distribution in epidemics of Mycobacterium tuberculosis

   This article has 1 author:

   1. Fabrizio Menardo

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is an interesting simulation-based study focusing on the genomic epidemiology of Mycobacterium tuberculosis. The work nicely relates key biological and epidemiological parameters to how M. tuberculosis isolates cluster together, and to the terminal branch lengths in M. tuberculosis phylogenies. These concepts have both been applied to comparative studies of M. tuberculosis success and have often been interpreted as reflecting differences in transmission. The author finds that clustering and terminal branch lengths can also be modified by differences in the latent period, the mutation rate or the sampling fraction. This work will be of broad interest to readers studying tuberculosis epidemiology and transmission modelling.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Botulinum neurotoxin accurately separates tonic vs. phasic transmission and reveals heterosynaptic plasticity rules in Drosophila

    This article has 7 authors:

    1. Yifu Han
    2. Chun Chien
    3. Pragya Goel
    4. Kaikai He
    5. Cristian Pinales
    6. Christopher Buser
    7. Dion Dickman

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The authors describe a new tool to completely block both evoked and spontaneous release in a selective manner using the GAL4 system in Drosophila. Surprisingly, they see no effects on either pre or postsynaptic development when both evoked and spontaneous release are blocked, in contrast to prior studies in the field. Overall, the results are provocative and will be of interest to the field. The study contradicts a number of published works, so it is important for the field to see the data and be able to evaluate it themselves.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife