eLife

Latest preprint reviews

1. Thermosynechococcus switches the direction of phototaxis by a c-di-GMP-dependent process with high spatial resolution

   This article has 6 authors:

   1. Daisuke Nakane
   2. Gen Enomoto
   3. Heike Bähre
   4. Yuu Hirose
   5. Annegret Wilde
   6. Takayuki Nishizaka

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript by Nakane et al investigates phototaxis of the rod shaped bacteria Thermosynechococcus vulcanus. This is important because most our knowledge on phototaxis is only emerging on round-shaped cyanobacteria. In the study, the authors demonstrate that T. vulcanus can chemotax positively or negatively to light depending on the light source. They identify a photoreceptor complex that drives negative phototaxis and propose that it impacts motility by increasing cdiGMP levels, which in turn would regulate the motility complex, formed by bi-polar Type-IV pili. Provided that the link between light induced cdi-GMP and spatial TFP activity is established, the work would provide a new mechanistic framework to explain TFP-driven phototaxis. This is an important topic because TFPs are emerging as spatially-regulated motility machineries in a large number of bacterial systems and linking their activity to receptors and secondary messengers is a current unresolved question.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. The metabolome of Mexican cavefish shows a convergent signature highlighting sugar, antioxidant, and Ageing-Related metabolites

   This article has 8 authors:

   1. J Kyle Medley
   2. Jenna Persons
   3. Tathagata Biswas
   4. Luke Olsen
   5. Robert Peuß
   6. Jaya Krishnan
   7. Shaolei Xiong
   8. Nicolas Rohner

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Medley et al. study A. mexicanus, an extreme-adapted organism with important connections to human health. The authors test metabolic responses in this natural model of elevated blood glucose and extensive body fat deposits, conditions generally expected to predispose to higher risk for metabolic syndrome and higher frailty. The work is rigorous and will provide a reference for future studies aimed at dissecting the mechanistic basis underlying metabolic shifts in this uniquely attractive model. The authors also provide an open and accessible window into their data and analyses by sharing a Shiny app.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Long-range migration of centrioles to the apical surface of the olfactory epithelium

   This article has 3 authors:

   1. Kaitlin Ching
   2. Jennifer T Wang
   3. Tim Stearns

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Analyzing the long-distance migration of centrioles to the dendrite tip of multicilated olfactory neurons in mice, Ching et al. use expansion microscopy to show that centrioles migrate as clusters, which mature as they reach the apical surface. The super-resolution data are impressive and the claims are generally supported by the data. Although the manuscript is largely descriptive, it is an important addition to the field, and will be of broad interest to cell biologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife, preLights

4. A-type FHFs mediate resurgent currents through TTX-resistant voltage-gated sodium channels

   This article has 6 authors:

   1. Yucheng Xiao
   2. Jonathan W Theile
   3. Agnes Zybura
   4. Yanling Pan
   5. Zhixin Lin
   6. Theodore R Cummins

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an exciting and important study that constitutes a major advance in the molecular understanding of resurgent Na current. Reproducing resurgent current by expression of two proteins has never been done. Here, the authors have for the first time molecularly reconstituted Na channels that produce resurgent Na current. Not only do these experiments satisfactorily and convincingly address a long-standing question in the field, but they also open the door to molecular manipulation of this current, potentially of significant practical use given the proposed role of the current in several disorders and disease states, including pain. The work will be of interest to many neuroscientists.

   Reviewed by eLife

5. Deciphering a hexameric protein complex with Angstrom optical resolution

   This article has 3 authors:

   1. Hisham Mazal
   2. Franz-Ferdinand Wieser
   3. Vahid Sandoghdar

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to the structural biology community and people working on cryogenic fluorescence microscopy. This paper is a clear step forward in the use of single-molecule localization microscopy at angstrom resolution, thanks to low-temperature polarized super-resolution imaging and advanced data processing algorithms.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

6. Inner membrane complex proteomics reveals a palmitoylation regulation critical for intraerythrocytic development of malaria parasite

   This article has 9 authors:

   1. Pengge Qian
   2. Xu Wang
   3. Chuan-Qi Zhong
   4. Jiaxu Wang
   5. Mengya Cai
   6. Wang Nguitragool
   7. Jian Li
   8. Huiting Cui
   9. Jing Yuan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to scientists within the field of apicomplexans cytoskeleton and malaria parasite proliferation. A series of compelling experimental manipulations identify potential new pellicle proteins and dissect the role of a protein acyl-transferase for the development of the intraerythrocytic stages of Plasmodiun yoelii and the palmitoylation status of two potential substrates.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Landscape of epithelial–mesenchymal plasticity as an emergent property of coordinated teams in regulatory networks

   This article has 5 authors:

   1. Kishore Hari
   2. Varun Ullanat
   3. Archana Balasubramanian
   4. Aditi Gopalan
   5. Mohit Kumar Jolly

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this paper, the authors identify topological metrics in gene-regulatory networks that potentially predict the kinds of phenotypic steady-states that the network allows. In particular, they apply their results to the epithelial-mesenchymal plasticity, showing that the relevant gene regulatory networks are structured as ‘teams' that may be 'strong', yielding stable phenotypes, or 'weak', yielding unstable phenotypes prone to plasticity. The work would be of interest to researchers interested in systems biology and the nonlinear dynamics of biological systems, as well as biologists interested in gene regulatory networks and their (mis)functioning in cancer cells.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

   This article has 35 authors:

   1. Lyra O Randzavola
   2. Paige M Mortimer
   3. Emma Garside
   4. Elizabeth R Dufficy
   5. Andrea Schejtman
   6. Georgia Roumelioti
   7. Lu Yu
   8. Mercedes Pardo
   9. Kerstin Spirohn
   10. Charlotte Tolley
   11. Cordelia Brandt
   12. Katherine Harcourt
   13. Esme Nichols
   14. Mike Nahorski
   15. Geoff Woods
   16. James C Williamson
   17. Shreehari Suresh
   18. John M Sowerby
   19. Misaki Matsumoto
   20. Celio XC Santos
   21. Cher Shen Kiar
   22. Subhankar Mukhopadhyay
   23. William M Rae
   24. Gordon J Dougan
   25. John Grainger
   26. Paul J Lehner
   27. Michael A Calderwood
   28. Jyoti Choudhary
   29. Simon Clare
   30. Anneliese Speak
   31. Giorgia Santilli
   32. Alex Bateman
   33. Kenneth GC Smith
   34. Francesca Magnani
   35. David C Thomas

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study focus follows this group's previous work on EROS and NOX2. In this current study the authors examine neutrophil EROS in the generation of superoxide by the NADPH oxidase. They demonstrate how EROS is involved in the maturation of gp91phox and expand our knowledge on the role of EROS in regulating expression of the P2x7 ion channel.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. Residual force enhancement is affected more by quadriceps muscle length than stretch amplitude

   This article has 4 authors:

   1. Patrick Bakenecker
   2. Tobias Weingarten
   3. Daniel Hahn
   4. Brent Raiteri

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors have systematically examined relationships between muscle length and force potentiation in young adults using very carefully conducted and controlled measurements by dynamometry and estimated using patellar tendon shear wave speed. The paper should be of interest to those who study human performance.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Time-resolved parameterization of aperiodic and periodic brain activity

    This article has 3 authors:

    1. Luc Edward Wilson
    2. Jason da Silva Castanheira
    3. Sylvain Baillet

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The paper addresses the highly timely questions of how to quantify aperiodic and periodic neural activity. This was done by extending previous work by embracing time-resolved parametrization of both simulated, noninvasive EEG and intracranial data. The new approach is termed Spectral Parametrization Resolved in Time (SPRiNT) and the paper shows that the slope of aperiodic activity is linked with both behaviour and age. The method thus demonstrates the importance of evaluating the state-dependence of aperiodic activity and dynamic properties of oscillatory components in a time-resolved manner.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife