eLife

Latest preprint reviews

1. Gallbladder adenocarcinomas undergo subclonal diversification and selection from precancerous lesions to metastatic tumors

   This article has 21 authors:

   1. Minsu Kang
   2. Hee Young Na
   3. Soomin Ahn
   4. Ji-Won Kim
   5. Sejoon Lee
   6. Soyeon Ahn
   7. Ju Hyun Lee
   8. Jeonghwan Youk
   9. Haesook T Kim
   10. Kui-Jin Kim
   11. Koung Jin Suh
   12. Jun Suh Lee
   13. Se Hyun Kim
   14. Jin Won Kim
   15. Yu Jung Kim
   16. Keun-Wook Lee
   17. Yoo-Seok Yoon
   18. Jee Hyun Kim
   19. Jin-Haeng Chung
   20. Ho-Seong Han
   21. Jong Seok Lee

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors collected human samples from a rare cancer type in which evolutionary features have not been well-defined. They describe the clonal evolution through sampling at precancerous, primary tumour, and metastatic stages. Whole exome sequencing was performed and one of the mutation types was confirmed with other techniques.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. A systematic, complexity-reduction approach to dissect the kombucha tea microbiome

   This article has 3 authors:

   1. Xiaoning Huang
   2. Yongping Xin
   3. Ting Lu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work will be of interest for researchers studying the functions of microbial communities, microbial ecology and interactions. Using the Kombucha tea (KT) microbiome as a case study, Huang et al. provide a framework for simplifying complex communities into core communities that capture aspects of complex communities. Authors demonstrated that core communities can facilitate a mechanistic understanding of how microbes interact, especially when member species are individually culturable. The work presents a fresh, novel approach for the coarse-grained analysis of complex microbiomes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Inhibitory IL-10-producing CD4+ T cells are T-bet-dependent and facilitate cytomegalovirus persistence via coexpression of arginase-1

   This article has 17 authors:

   1. Mathew Clement
   2. Kristin Ladell
   3. Kelly L Miners
   4. Morgan Marsden
   5. Lucy Chapman
   6. Anna Cardus Figueras
   7. Jake Scott
   8. Robert Andrews
   9. Simon Clare
   10. Valeriia V Kriukova
   11. Ksenia R Lupyr
   12. Olga V Britanova
   13. David R Withers
   14. Simon A Jones
   15. Dmitriy M Chudakov
   16. David A Price
   17. Ian R Humphreys

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript analyses the inhibitory role of IL-10 producing regulatory T-cells in a mouse cytomegalovirus infection model. The authors report that IL-10 producing CD4+T-cells express genes of chronically activated Th1-cells, are clonally expanded and inhibit anti-viral T-cell responses via arginase, an enzyme that breaks down an essential amino acid for T-cell activation. The manuscript presents some novel and potentially important data; however, it requires the provision of additional experimental data and clarifications.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Arbidol inhibits human esophageal squamous cell carcinoma growth in vitro and in vivo through suppressing ataxia telangiectasia and Rad3-related protein kinase

   This article has 13 authors:

   1. Ning Yang
   2. Xuebo Lu
   3. Yanan Jiang
   4. Lili Zhao
   5. Donghao Wang
   6. Yaxing Wei
   7. Yin Yu
   8. Myoung Ok Kim
   9. Kyle Vaughn Laster
   10. Xin Li
   11. Baoyin Yuan
   12. Zigang Dong
   13. Kangdong Liu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to a broad audience of cancer biologists, especially those interested in esophageal cancer or treatment strategies involving ATR inhibition. It provides novel information about how FDA-approved antiretroviral compound Arbidol is a potential ATR inhibitor, which is of interest in the treatment of multiple tumor types. The key claims of the manuscript are supported by in silico, in vitro, and in vivo data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

5. Label-free three-photon imaging of intact human cerebral organoids for tracking early events in brain development and deficits in Rett syndrome

   This article has 11 authors:

   1. Murat Yildirim
   2. Chloe Delepine
   3. Danielle Feldman
   4. Vincent A Pham
   5. Stephanie Chou
   6. Jacque Ip
   7. Alexi Nott
   8. Li-Huei Tsai
   9. Guo-Li Ming
   10. Peter TC So
   11. Mriganka Sur

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to stem cell and developmental biologists who aim to use newly emerging brain organoid models to understand the structure and function of the developing human brain. It presents a technological advance in imaging and describes an innovative method for labeling and tracking of cells within organoids to enable the assessment of dynamic processes within the intact organoid. The method is validated in a disease model and addresses a challenge in the field of human stem cell modeling of assessing cells within the 3D structure.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. The Candida albicans virulence factor candidalysin polymerizes in solution to form membrane pores and damage epithelial cells

   This article has 13 authors:

   1. Charles M Russell
   2. Katherine G Schaefer
   3. Andrew Dixson
   4. Amber LH Gray
   5. Robert J Pyron
   6. Daiane S Alves
   7. Nicholas Moore
   8. Elizabeth A Conley
   9. Ryan J Schuck
   10. Tommi A White
   11. Thanh D Do
   12. Gavin M King
   13. Francisco N Barrera

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript is of interest to several fields, in particular to microbiologists and structural biologists interested in pore-forming proteins and peptides. The data presented reveal insights into the mode of action of a newly identified peptide toxin secreted by Candida albicans (candidalysin). Using different techniques the authors propose and test a model for membrane perforation by candidalysin and identify an intriguing inactive mutant. While the presented data supports the main conclusions of the paper some of the initial assumptions need further assessment while the described mutants could benefit from more extensive characterization.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Design of an optimal combination therapy with broadly neutralizing antibodies to suppress HIV-1

   This article has 6 authors:

   1. Colin LaMont
   2. Jakub Otwinowski
   3. Kanika Vanshylla
   4. Henning Gruell
   5. Florian Klein
   6. Armita Nourmohammad

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study provides computational predictions on optimal combinations of broadly neutralizing antibodies for treating HIV-1, based on the finding that population diversity alone permits the prediction of the timing of viral escape from broadly neutralizing antibodies. The idea behind the approach used is good, although the analyses and computational data/results highlight important limitations of the modeling approach. Nonetheless, the study should be of broad interest to those studying viral responses to therapeutic interventions as well as to both evolutionary and computational biologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. DNALI1 interacts with the MEIG1/PACRG complex within the manchette and is required for proper sperm flagellum assembly in mice

   This article has 14 authors:

   1. Yi Tian Yap
   2. Wei Li
   3. Qian Huang
   4. Qi Zhou
   5. David Zhang
   6. Yi Sheng
   7. Ljljiana Mladenovic-Lucas
   8. Siu-Pok Yee
   9. Kyle E Orwig
   10. James G Granneman
   11. David C Williams
   12. Rex A Hess
   13. Aminata Toure
   14. Zhibing Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of potential interest to a broad audience in the fields of germ cell biology and cytoskeleton, as it implies a microtubule-based motor function in intra-manchette cargo transport in developing sperm tail. However, some conclusions of this paper require stronger experimental support.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

9. Vocalization categorization behavior explained by a feature-based auditory categorization model

   This article has 8 authors:

   1. Manaswini Kar
   2. Marianny Pernia
   3. Kayla Williams
   4. Satyabrata Parida
   5. Nathan Alan Schneider
   6. Madelyn McAndrew
   7. Isha Kumbam
   8. Srivatsun Sadagopan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study combines behavioral data from guinea pigs and data from a classifier model to ask what auditory features are important for classifying vocalisations. This study is likely to be of interest to both computational and experimental neuroscientists, in particular auditory neurophysiologists and cognitive and comparative neuroscientists. A strength of this work is that a model trained on natural calls was able to predict some aspects of responses to temporally and spectrally altered cues. However, additional data, analysis, or modelling would be required to support some of the stronger claims.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

10. Effective mechanical potential of cell–cell interaction explains three-dimensional morphologies during early embryogenesis

    This article has 7 authors:

    1. Hiroshi Koyama
    2. Hisashi Okumura
    3. Atsushi M. Ito
    4. Kazuyuki Nakamura
    5. Tetsuhisa Otani
    6. Kagayaki Kato
    7. Toshihiko Fujimori

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      In this manuscript, effective force-distance curves between cells are inferred for various tissues. This study is potentially interesting for researchers interested in tissue dynamics, because computer models of growing cellular tissues are becoming an increasingly important tool to understand experimental data and eventually predict medical interventions.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife