eLife

Latest preprint reviews

1. Associative learning drives longitudinally graded presynaptic plasticity of neurotransmitter release along axonal compartments

   This article has 11 authors:

   1. Aaron Stahl
   2. Nathaniel C Noyes
   3. Tamara Boto
   4. Valentina Botero
   5. Connor N Broyles
   6. Miao Jing
   7. Jianzhi Zeng
   8. Lanikea B King
   9. Yulong Li
   10. Ronald L Davis
   11. Seth M Tomchik

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to scientists working on learning and memory and synaptic plasticity. It provides a useful overview of different forms of plasticity taking place in the learning and memory center of the fly, the mushroom body. The study mostly uses an acetylcholine sensor to image activity, which is novel and helps to tie together previous studies reporting memory-induced changes in calcium transients. In particular, the study highlights the compartmentalised plasticity along Kenyon cell axon terminals, the main cell type of the mushroom body. The current version of the manuscript could be improved by including some key issues: (1) behavioral experiments for the Cac knock-down experiments, (2) specific controls for some of the imaging experiments, (3) consideration of the role of dopaminergic neurons and (4) acknowledgment of the complexity of the mushroom body circuit and the literature that has addressed this previously.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. State-dependent representations of mixtures by the olfactory bulb

   This article has 4 authors:

   1. Aliya Mari Adefuin
   2. Sander Lindeman
   3. Janine Kristin Reinert
   4. Izumi Fukunaga

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study examined how mixture odors are represented in the mouse olfactory bulb. The authors found that compared to the responses in anesthetized mice, mixture responses are more linear in awake mice regardless whether the mice were engaged in a behavioral task or not. The results are potentially important as the results differ from previous studies which were done mostly in anesthetized animals, but the reviewers raised concerns for the validity and the strength of the conclusions.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Confined migration promotes cancer metastasis through resistance to anoikis and increased invasiveness

   This article has 14 authors:

   1. Deborah Fanfone
   2. Zhichong Wu
   3. Jade Mammi
   4. Kevin Berthenet
   5. David Neves
   6. Kathrin Weber
   7. Andrea Halaburkova
   8. François Virard
   9. Félix Bunel
   10. Catherine Jamard
   11. Hector Hernandez-Vargas
   12. Stephen WG Tait
   13. Ana Hennino
   14. Gabriel Ichim

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The study proposes that confined migration renders breast cancer cells resistant to apoptosis via NFkappaB-dependent mechanisms. The technical aspects of the study are impressive and experiments are very well performed and demonstrate the value of mimetic bioengineering approaches, but the postulated central premise would require more rigorous support.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

4. Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses

   This article has 4 authors:

   1. Lisa AE Catsburg
   2. Manon Westra
   3. Annemarie ML van Schaik
   4. Harold D MacGillavry

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Catsburg et al. provide a new descriptive characterization of clathrin structures in spines vs dendrites using an excellent knock-in approach they recently developed. These results carefully validate earlier findings using the CRISPR approach and constitute useful baseline information, which would be useful in examining changes in the zone induced by neuronal activity or synaptic plasticity.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)

   This article has 6 authors:

   1. Daniel W McKay
   2. Heather E McFarlane
   3. Yue Qu
   4. Apriadi Situmorang
   5. Matthew Gilliham
   6. Stefanie Wege

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Presented here is a study on the role of cation chloride cotransporter CCC1 as a key regulator of the plant Trans-Golgi/Early Endosome trafficking network. While the work is well controlled and presented overall, the reviewers judged the data supporting localization of CCC1 to TGN/EE as not being sufficiently clear, as was the role of CCC1 in endocytosis, which is one of the main conclusions. These points can be clarified with future careful experimentation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Telencephalic outputs from the medial entorhinal cortex are copied directly to the hippocampus

   This article has 7 authors:

   1. Sau Yee Tsoi
   2. Merve Öncül
   3. Ella Svahn
   4. Mark Robertson
   5. Zuzanna Bogdanowicz
   6. Christina McClure
   7. Gülşen Sürmeli

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to readers in the field of anatomy and hippocampal physiology. The authors provide characterization of a novel projection pathway from layer 5a neurons in the MEC to CA1 of the hippocampus. They utilize cell specific viral labelling techniques, RNA-sequencing based projection mapping, and optogenetic aided in vitro physiology. The anatomical conclusions are well supported by the data. Future functional experiments will be necessary to establish the functional role of the projection.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

7. Plant SYP12 syntaxins mediate an evolutionarily conserved general immunity to filamentous pathogens

   This article has 4 authors:

   1. Hector M Rubiato
   2. Mengqi Liu
   3. Richard J O'Connell
   4. Mads E Nielsen

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The study provides evidence that PEN1 and SYP122 regulate defense structures against filamentous pathogen infection including papillae formation and encasement of haustoria; this appears to be an ancient defense mechanism in land plants. If the findings that PEN1 and its close homolog SYP122 play an overlapping role in pre- and post-invasive immunity against cell-wall penetrating filamentous pathogens could be further validated, this would advance our understanding of callosic papilla/encasement-based nonhost defense mechanisms.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Connexin hemichannels with prostaglandin release in anabolic function of bone to mechanical loading

   This article has 7 authors:

   1. Dezhi Zhao
   2. Manuel A Riquelme
   3. Teja Guda
   4. Chao Tu
   5. Huiyun Xu
   6. Sumin Gu
   7. Jean X Jiang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors have used two transgenic mouse models expressing dominant negative Cx43 mutants to evaluate the role of Cx43 hemichannels in mechanical loading response in bone. While understanding the molecular mechanisms by which osteocytes respond to mechanical strain is of interest in the skeletal biology arena, the conclusions of this study are not fully supported by experimental data and are of only incremental in nature.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Control of Parallel Hippocampal Output Pathways by Amygdalar Long-Range Inhibition

   This article has 7 authors:

   1. Rawan AlSubaie
   2. Ryan W S Wee
   3. Anne Ritoux
   4. Karyna Mishchanchuk
   5. Jessica Passlack
   6. Daniel Regester
   7. Andrew F MacAskill

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript suggests that the neuronal circuit from the basal amygdala (BA) to the ventral hippocampus (VH) consists of both excitatory and inhibitory projections to specific targeted areas. The main message is that excitatory input specifically targets VH neurons that in turn project to the BA and nucleus accumbens (NA). In contrast, BA inhibitory input preferentially targets VH neurons that project to the BA to gate place-value associations. The reviewers agree that the manuscript reports potentially interesting data. However, they all agree that the claim made is preliminary and only partially sustained by the submitted experimental evidence.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

10. Neural correlates and determinants of approach–avoidance conflict in the prelimbic prefrontal cortex

    This article has 6 authors:

    1. Jose A Fernandez-Leon
    2. Douglas S Engelke
    3. Guillermo Aquino-Miranda
    4. Alexandria Goodson
    5. Maria N Rasheed
    6. Fabricio H Do Monte

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The study of cortical regulation of behavior under conflict is novel, timely, and important. It is of broad interest to neuroscientists studying the neural substrates of fear and reward and offers a novel behavioural perspective showing how these opposing motivational states interact to influence behaviour differentially across individuals and how this is regulated by glutamatergic and GABAergic neurons in the prefrontal cortex. The paper uses a variety of cutting-edge tools to dissect the microcircuits of the prefrontal cortex. The claims are generally supported by the data, but some claims comparing individual differences require additional statistical analyses and consideration of potential alternative interpretations for the behavioral phenotypes observed.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife