eLife

Latest preprint reviews

1. Cytotoxic CD4+ T cells driven by T-cell intrinsic IL-18R/MyD88 signaling predominantly infiltrate Trypanosoma cruzi-infected hearts

   This article has 12 authors:

   1. Carlos-Henrique D Barbosa
   2. Fábio B Canto
   3. Ariel Gomes
   4. Layza M Brandao
   5. Jéssica R Lima
   6. Guilherme A Melo
   7. Alessandra Granato
   8. Eula GA Neves
   9. Walderez O Dutra
   10. Ana-Carolina Oliveira
   11. Alberto Nóbrega
   12. Maria Bellio

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is an interesting study, conducted in mice, that demonstrates for the first time the presence of a large population of cytotoxic CD4+ T lymphocytes in infection with Trypanosoma cruzi, a relevant human pathogen. At present, the relevance of these cells in protective immunity engendered by the host remains unclear. Additional experiments are needed to characterize the functionality of these cytotoxic CD4 T cells vis-a-vis the canonical Th1 T cells. This paper can be of interest to scientists interested in immune responses to parasitic infections.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. Redox-controlled reorganization and flavin strain within the ribonucleotide reductase R2b–NrdI complex monitored by serial femtosecond crystallography

   This article has 27 authors:

   1. Juliane John
   2. Oskar Aurelius
   3. Vivek Srinivas
   4. Patricia Saura
   5. In-Sik Kim
   6. Asmit Bhowmick
   7. Philipp S Simon
   8. Medhanjali Dasgupta
   9. Cindy Pham
   10. Sheraz Gul
   11. Kyle D Sutherlin
   12. Pierre Aller
   13. Agata Butryn
   14. Allen M Orville
   15. Mun Hon Cheah
   16. Shigeki Owada
   17. Kensuke Tono
   18. Franklin D Fuller
   19. Alexander Batyuk
   20. Aaron S Brewster
   21. Nicholas K Sauter
   22. Vittal K Yachandra
   23. Junko Yano
   24. Ville RI Kaila
   25. Jan Kern
   26. Hugo Lebrette
   27. Martin Högbom

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript will be of interest to a broad audience in structural biology, biochemistry, and enzymology. The work demonstrates the use of a cutting-edge approach in protein crystallography to investigate and visualize the complex mechanism of an enzyme, the paradigm being Mn-dependent ribonucleotide reductase R2b in complex with flavin-bound NrdI at different redox states. The work is timely and has implications for future investigation of complex biochemical processes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Tree species and genetic diversity increase productivity via functional diversity and trophic feedbacks

   This article has 18 authors:

   1. Ting Tang
   2. Naili Zhang
   3. Franca J Bongers
   4. Michael Staab
   5. Andreas Schuldt
   6. Felix Fornoff
   7. Hong Lin
   8. Jeannine Cavender-Bares
   9. Andrew L Hipp
   10. Shan Li
   11. Yu Liang
   12. Baocai Han
   13. Alexandra-Maria Klein
   14. Helge Bruelheide
   15. Walter Durka
   16. Bernhard Schmid
   17. Keping Ma
   18. Xiaojuan Liu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Using an impressive experimental design, Tang et al. analyzed the effects of intraspecific (genetic) and interspecific (species) diversity in ecosystem processes carried out by forest communities. The results show that both species and genotype diversity influence productivity via changes in overall functional diversity, herbivory, and soil fungal diversity. This study will be important to ecologists and environmentalists interested in ecosystem processes and restoration efforts.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Centrally expressed Cav3.2 T-type calcium channel is critical for the initiation and maintenance of neuropathic pain

   This article has 14 authors:

   1. Sophie L Fayad
   2. Guillaume Ourties
   3. Benjamin Le Gac
   4. Baptiste Jouffre
   5. Sylvain Lamoine
   6. Antoine Fruquière
   7. Sophie Laffray
   8. Laila Gasmi
   9. Bruno Cauli
   10. Christophe Mallet
   11. Emmanuel Bourinet
   12. Thomas Bessaih
   13. Régis C Lambert
   14. Nathalie Leresche

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript shows an important role for Cav2.3 channels in SNI-mediated allodynia and firing properties of PV-expressing APT neurons. Mechanisms that underlie adaptations in chronic pain models are extremely important for the development of novel therapeutics for chronic pain and this could be a significant contribution in that regard. However, the discussion asserts that these studies are the "first direct evidence that supra-spinal Cav3.2 channels play a fundamental role in pain pathophysiology." This is an overstatement as Chen and colleagues examined the role of these channels in the anterior cingulate cortex in CCI-mediated neuropathic pain (Shen, et al., 2015, Molecular Pain).

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. GPR183 mediates the capacity of the novel CD47-CD19 bispecific antibody TG-1801 to heighten ublituximab-umbralisib (U2) anti-lymphoma activity

   This article has 12 authors:

   1. Marcelo Lima Ribeiro
   2. Núria Profitós-Pelejà
   3. Juliana Carvalho Santos
   4. Pedro Blecua
   5. Diana Reyes Garau
   6. Marc Armengol
   7. Miranda Fernández-Serrano
   8. Hari P. Miskin
   9. Francesc Bosch
   10. Manel Esteller
   11. Emmanuel Normant
   12. Gael Roué

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Ribeiro M et al investigate the ability of a novel bispecific CD19-CD47 antibody to enhance the cell mediated killing mediated by existing drug combinations - anti-CD20 plus PIK3d/CK1E inhibitor. The novelty of this study is the restriction to CD19 positive lymphoma cells, thus potentially avoiding toxicity to non-lymphoma lineages, and the gene expression profiling to identify up regulation of GPR183 after combined treatment of CD19/47 plus CD20/PI3K/CK1E vs CD19/47 alone. Genetic and drug studies suggest that GPR183 is essential for the full activity of the triplet drug combination.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. Neural dynamics of causal inference in the macaque frontoparietal circuit

   This article has 5 authors:

   1. Guangyao Qi
   2. Wen Fang
   3. Shenghao Li
   4. Junru Li
   5. Liping Wang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation summary:

     This study investigates the neural basis of the hidden causal structure between visual and proprioceptive signals in the primate premotor and parietal circuit during reaching tasks executed in a virtual reality environment, where information between the two modalities can be dissociated. Modelling is used to characterize the proprioceptive drift of the monkeys when integrating bimodal information. The key novel result is that premotor neurons represent the integration of bimodal information for small disparities and the segregation for large disparities between the proprioceptive and visual information, while parietal cells show reaching tuning changes that support the updating sensory uncertainty between tasks. Overall, the experiments are technically sound, and the conclusions are mostly well supported. However, a simpler framing of the paper could make the main message easier to grasp, the analysis of Bayesian models seems to lack major details, the statistical reporting is below standard, and a large part of the extensive literature on the role of premotor and parietal cortex in visuomotor behavior is lacking.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

7. β2-subunit alternative splicing stabilizes Cav2.3 Ca2+ channel activity during continuous midbrain dopamine neuron-like activity

   This article has 19 authors:

   1. Anita Siller
   2. Nadja T Hofer
   3. Giulia Tomagra
   4. Nicole Burkert
   5. Simon Hess
   6. Julia Benkert
   7. Aisylu Gaifullina
   8. Desiree Spaich
   9. Johanna Duda
   10. Christina Poetschke
   11. Kristina Vilusic
   12. Eva Maria Fritz
   13. Toni Schneider
   14. Peter Kloppenburg
   15. Birgit Liss
   16. Valentina Carabelli
   17. Emilio Carbone
   18. Nadine Jasmin Ortner
   19. Jörg Striessnig

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper suggests that assembly of CaV2.3 with b2a/b2e splice variants confers biophysical properties that enable these channels to contribute to calcium-dependent pacemaking in dopaminergic neurons. The findings could have implications for why these neurons are vulnerable to degeneration in Parkinson's disease. The work will be of interest to ion channel biophysicists and neuroscientists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. Vision-related convergent gene losses reveal SERPINE3’s unknown role in the eye

   This article has 10 authors:

   1. Henrike Indrischek
   2. Juliane Hammer
   3. Anja Machate
   4. Nikolai Hecker
   5. Bogdan Kirilenko
   6. Juliana Roscito
   7. Stefan Hans
   8. Caren Norden
   9. Michael Brand
   10. Michael Hiller

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors use a comparative genomics approach to predict gene function, in particular genes that have a role in eye development. After identifying the convergent loss of SERPINE3 with vision loss across mammals, the authors confirmed its involvement in eye development by characterizing zebrafish knockouts. This work highlights the power of comparative genomics to generate hypotheses that can be experimentally validated. This work is relevant to a broad audience interested in evolution and adaptation as well as for those studying eye development and eye pathologies.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

9. Transcriptome network analysis implicates CX3CR1-positive type 3 dendritic cells in non-infectious uveitis

   This article has 11 authors:

   1. Sanne Hiddingh
   2. Aridaman Pandit
   3. Fleurieke Verhagen
   4. Rianne Rijken
   5. Nila Hendrika Servaas
   6. Rina CGK Wichers
   7. Ninette H ten Dam-van Loon
   8. Saskia M Imhof
   9. Timothy RDJ Radstake
   10. Joke H de Boer
   11. Jonas JW Kuiper

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors provide evidence suggesting the gene expression profile of a specific subset of dendritic cells define features of specific forms of non-infectious uveitis. This work suggests specific pathways in these cells that may have mechanistic import in inflammatory eye disease. This manuscript is of interested to immunologists studying autoimmunity and ocular immunity. While the paper is largely descriptive, the data it presents should serve as a valuable resource for generating hypotheses about the pathogenesis of ocular autoimmune disease.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Robust and Efficient Assessment of Potency (REAP) as a quantitative tool for dose-response curve estimation

    This article has 9 authors:

    1. Shouhao Zhou
    2. Xinyi Liu
    3. Xinying Fang
    4. Vernon M Chinchilli
    5. Michael Wang
    6. Hong-Gang Wang
    7. Nikolay V Dokholyan
    8. Chan Shen
    9. J Jack Lee

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This article proposes methodology and accompanying software for robustly fitting dose-response curves where response is a number between 0 and 1. When response is transformed using the common logistic transformation, values close to 0 or 1 become large in magnitude, unduly influencing the fitted curve after back-transformation and introducing bias in the estimate of certain parameters. The proposed approach, called Robust and Efficient Assessment of Potency, is less perturbed by these extreme measurements.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife