eLife

Latest preprint reviews

1. Variation in the ACE2 receptor has limited utility for SARS-CoV-2 host prediction

   This article has 5 authors:

   1. Nardus Mollentze
   2. Deborah Keen
   3. Uuriintuya Munkhbayar
   4. Roman Biek
   5. Daniel G Streicker

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This important study shows that methods currently used to predict which animals species might be at risk of infection by SARS-CoV-2, by looking at features of the host cell receptor the virus binds to, are fundamentally flawed, with exceptionally strong support for this conclusion. Much work on the potential host range of SARS-CoV-2 has focused on measuring the susceptibility of different species' ACE2 receptors to sarbecovirus entry and extending predictions to other unmeasured species based on ACE2 sequence features. Mollentze and colleagues show that ACE2 sequences are not more than a proxy for generic species relationships. In other words, species phylogeny alone can provide equivalent predictive power, allowing for predictions of mammalian susceptibility to sarbecovirus infection for the many species for which ACE2 sequences are not known yet.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

2. A novel fold for acyltransferase-3 (AT3) proteins provides a framework for transmembrane acyl-group transfer

   This article has 6 authors:

   1. Kahlan E Newman
   2. Sarah N Tindall
   3. Sophie L Mader
   4. Syma Khalid
   5. Gavin H Thomas
   6. Marjan W Van Der Woude

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     By integrating a range of computational techniques, the authors generated a structural model for the AT3 domain, which is predicted to adopt a new fold. The key features of the structural model are consistent with the activity of the enzyme as an acyltransferase, with a transmembrane channel that can accommodate an acyl-CoA donor, and an outer cavity formed with a second domain that can accommodate a nascent LPS molecule as substrate. Overall, the study will help stimulate specific experimental analyses that can further evaluate and improve the model for better mechanistic understanding of this class of enzymes. The work will be of interest to structural biologists, and all studying acyltransferase enzymes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. A novel monocyte differentiation pattern in pristane-induced lupus with diffuse alveolar hemorrhage

   This article has 4 authors:

   1. Shuhong Han
   2. Haoyang Zhuang
   3. Rawad Daniel Arja
   4. Westley H Reeves

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors dissect the phenotypes of bone-marrow derived myeloid cells in a murine model of pulmonary vasculitis with relevance to human disease, revealing the association of novel phenotypic subsets associated with lung injury, yet the role of these subsets in regulating or contributing to tissue injury is less clearly determined.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewer remained anonymous to the authors.)

   Reviewed by eLife

4. High spatial resolution analysis using automated indentation mapping differentiates biomechanical properties of normal vs. degenerated articular cartilage in mice

   This article has 4 authors:

   1. Anand O Masson
   2. Bryce Besler
   3. W Brent Edwards
   4. Roman J Krawetz

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The work presented by Masson et al. highlights experimental approaches using spatial indentation and contrast-enhanced 3-D x-ray imaging to topographically map cartilage thickness in mouse knee joints. This methods described have the potential to impact the field of musculoskeletal biomechanics, especially for researchers using mouse models to study cartilage wear and disease, given the high resolution and sensitivity of the described approaches.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Integration of mouse ovary morphogenesis with developmental dynamics of the oviduct, ovarian ligaments, and rete ovarii

   This article has 6 authors:

   1. Jennifer McKey
   2. Dilara N Anbarci
   3. Corey Bunce
   4. Alejandra E Ontiveros
   5. Richard R Behringer
   6. Blanche Capel

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work describes mouse ovary morphogenesis from E14.5 to birth using recently developed methods combining CUBIC and iDISCO and optimized 3D imaging using light sheet microscopy. The manuscript is of interest to all developmental biologists as it will serve as a reference to whole embryo morphogenesis, in particular vertebrate ovary morphogenetic processes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Transversal functional connectivity and scene-specific processing in the human entorhinal-hippocampal circuitry

   This article has 5 authors:

   1. Xenia Grande
   2. Magdalena M Sauvage
   3. Andreas Becke
   4. Emrah Düzel
   5. David Berron

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Grande and colleagues provide new insights into how different regions of the entorhinal cortex functionally interact with specific cortical brain areas and how, in turn, subregions of the entorhinal cortex interact with the hippocampus during 'scene' and 'object' processing. This paper is relevant to cognitive neuroscientists with an interest in the entorhinal cortex - hippocampal pathways and 'scene' and 'object' representation in the medial temporal lobe. The study is well-motivated, well-designed and appropriately analysed to address the research questions. Most conclusions of the paper are well supported by the data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Variation in ubiquitin system genes creates substrate-specific effects on proteasomal protein degradation

   This article has 3 authors:

   1. Mahlon A Collins
   2. Gemechu Mekonnen
   3. Frank Wolfgang Albert

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors use an elegant experimental design to study genetic variation in the ubiquitin-proteasome degradation system in yeast. They identify a large number of QTLs for naturally occurring variation, and they elucidate the causal variants and likely functional mechanisms of several of these. The paper illustrates an innovative new approach to high-throughput QTL mapping for specific molecular processes and it will be of interest to colleagues aiming to harness natural variation for understanding a range of biological processes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Differences in the immune response elicited by two immunization schedules with an inactivated SARS-CoV-2 vaccine in a randomized phase 3 clinical trial

   This article has 40 authors:

   1. Nicolás MS Gálvez
   2. Gaspar A Pacheco
   3. Bárbara M Schultz
   4. Felipe Melo-González
   5. Jorge A Soto
   6. Luisa F Duarte
   7. Liliana A González
   8. Daniela Rivera-Pérez
   9. Mariana Ríos
   10. Roslye V Berrios
   11. Yaneisi Vázquez
   12. Daniela Moreno-Tapia
   13. Omar P Vallejos
   14. Catalina A Andrade
   15. Guillermo Hoppe-Elsholz
   16. Carolina Iturriaga
   17. Marcela Urzua
   18. María S Navarrete
   19. Álvaro Rojas
   20. Rodrigo Fasce
   21. Jorge Fernández
   22. Judith Mora
   23. Eugenio Ramírez
   24. Aracelly Gaete-Argel
   25. Mónica L Acevedo
   26. Fernando Valiente-Echeverría
   27. Ricardo Soto-Rifo
   28. Daniela Weiskopf
   29. Alba Grifoni
   30. Alessandro Sette
   31. Gang Zeng
   32. Weining Meng
   33. CoronaVacCL03 Study Group
   34. José V González-Aramundiz
   35. Marina Johnson
   36. David Goldblatt
   37. Pablo A González
   38. Katia Abarca
   39. Susan M Bueno
   40. Alexis M Kalergis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript described the effects of two different CoronaVac vaccination schedules in a Chilean adult population. They find that a 0 and 28-day two-dose schedule produced superior levels of neutralizing antibodies and antibodies with a great breadth of interaction across variants compared to a 0 and 14-day two-dose schedule. They find no differences in T cell responses or total antibody levels between the two groups. These findings demonstrate that a short two-week two-dose interval should provide sufficient immunity to reduce the likelihood of serious outcomes during a COVID infection.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution

   This article has 37 authors:

   1. Geoffrey W Meissner
   2. Aljoscha Nern
   3. Zachary Dorman
   4. Gina M DePasquale
   5. Kaitlyn Forster
   6. Theresa Gibney
   7. Joanna H Hausenfluck
   8. Yisheng He
   9. Nirmala A Iyer
   10. Jennifer Jeter
   11. Lauren Johnson
   12. Rebecca M Johnston
   13. Kelley Lee
   14. Brian Melton
   15. Brianna Yarbrough
   16. Christopher T Zugates
   17. Jody Clements
   18. Cristian Goina
   19. Hideo Otsuna
   20. Konrad Rokicki
   21. Robert R Svirskas
   22. Yoshinori Aso
   23. Gwyneth M Card
   24. Barry J Dickson
   25. Erica Ehrhardt
   26. Jens Goldammer
   27. Masayoshi Ito
   28. Dagmar Kainmueller
   29. Wyatt Korff
   30. Lisa Mais
   31. Ryo Minegishi
   32. Shigehiro Namiki
   33. Gerald M Rubin
   34. Gabriella R Sterne
   35. Tanya Wolff
   36. Oz Malkesman
   37. FlyLight Project Team

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study bridges the gap between connectomic data from the fly hemibrain and driver lines needed for functional experiments through a new freely available computational tool, NeuronBridge. It demonstrates that this software provides users with the ability to identify the same neurons within different driver lines, and the opportunity to match expression of neurons in a driver line with those in a connectomic database. Overall, this manuscript does a commendable job of describing an important resource for the community, which will hopefully be built upon via collaborative science of many groups as the field develops.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymis

    This article has 17 authors:

    1. Christiane Pleuger
    2. Dingding Ai
    3. Minea L Hoppe
    4. Laura T Winter
    5. Daniel Bohnert
    6. Dominik Karl
    7. Stefan Guenther
    8. Slava Epelman
    9. Crystal Kantores
    10. Monika Fijak
    11. Sarina Ravens
    12. Ralf Middendorff
    13. Johannes U Mayer
    14. Kate L Loveland
    15. Mark Hedger
    16. Sudhanshu Bhushan
    17. Andreas Meinhardt

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This manuscript addresses a long-standing question regarding the highly variable cellular composition and functions as well as immune environments along the epididymis. Using multiple mouse models (bacterial infection and parabiosis between WT and Ccr2 KO) in conjunction with powerful scRNA-seq analyses, the authors provided solid evidence supporting the notion that resident immune cells are strategically positioned along the epididymal duct, potentially providing different immunological environments required for sperm maturations and elimination of pathogens ascending the urogenital tract.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife