eLife

Latest preprint reviews

1. Interrogating the precancerous evolution of pathway dysfunction in lung squamous cell carcinoma using XTABLE

   This article has 8 authors:

   1. Matthew Roberts
   2. Julia Ogden
   3. AS Mukarram Hossain
   4. Anshuman Chaturvedi
   5. Alastair RW Kerr
   6. Caroline Dive
   7. Jennifer Ellen Beane
   8. Carlos Lopez-Garcia

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors have developed a useful and user-friendly software to analyse gene expression data from four datasets representing premalignant lung lesions. This software would be of interest to those working in lung cancer and specifically the pre-malignant space. The major strength is the ease of use while the major limitation is the inability for the user to integrate other datasets.

   Reviewed by eLife

2. Shear and hydrostatic stress regulate fetal heart valve remodeling through YAP-mediated mechanotransduction

   This article has 6 authors:

   1. Mingkun Wang
   2. Belle Yanyu Lin
   3. Shuofei Sun
   4. Charles Dai
   5. Feifei Long
   6. Jonathan T Butcher

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Determination of the biomechanical forces and downstream pathways that direct heart valve morphogenesis is an important area of research. In the current study, potential functions of localized Yap signaling in cardiac valve morphogenesis were examined. However, the evidence for Yap pathway activation and localization relative to areas of the valve subject to different mechanical stresses is not convincing.

   Reviewed by eLife

3. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells

   This article has 8 authors:

   1. Payam E Farahani
   2. Xiaoyu Yang
   3. Emily V Mesev
   4. Kaylan A Fomby
   5. Ellen H Brumbaugh-Reed
   6. Caleb J Bashor
   7. Celeste M Nelson
   8. Jared E Toettcher

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a well-explained and potentially useful study that describes the generation and use of pYtags, recombinant proteins that, if properly used, should allow spatiotemporal monitoring of the activation of different receptor tyrosine kinases in living cells. Although this study has generated new tools to evaluate receptor localization and activation in different cells, the broad concept showing that different receptor dimers generate specific stimuli, and downstream signaling pathways, is quite limited in terms of novelty. Although it is felt that the study is technologically innovative, the analysis of receptor spatial signaling is incomplete and should be improved.

   Reviewed by eLife

4. Concurrent remodelling of nucleolar 60S subunit precursors by the Rea1 ATPase and Spb4 RNA helicase

   This article has 6 authors:

   1. Valentin Mitterer
   2. Matthias Thoms
   3. Robert Buschauer
   4. Otto Berninghausen
   5. Ed Hurt
   6. Roland Beckmann

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental study substantially advances our understanding of the process of ribosome maturation. The authors have purified and determined the structures of several nucleolar ribosome assembly intermediates in yeast using cryo-electron microscopy (cryo-EM). The study combines genetic, biochemical, and structural analysis to provide compelling support for the conclusions the authors wish to draw. The work will be of broad interest to cell biologists, biochemists, and structural biologists.

   Reviewed by eLife

5. Context-dependent requirement of G protein coupling for Latrophilin-2 in target selection of hippocampal axons

   This article has 6 authors:

   1. Daniel T Pederick
   2. Nicole A Perry-Hauser
   3. Huyan Meng
   4. Zhigang He
   5. Jonathan A Javitch
   6. Liqun Luo

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an intriguing study investigating the molecular mechanisms of neural circuit developmental organization. Using a defined hippocampal circuit, the authors find that ectopic expression of an adhesion G protein-receptor leads to axon mistargeting. This work defines new mechanisms of axon target specificity.

   Reviewed by eLife

6. Statistical inference reveals the role of length, GC content, and local sequence in V(D)J nucleotide trimming

   This article has 4 authors:

   1. Magdalena L Russell
   2. Noah Simon
   3. Philip Bradley
   4. Frederick A Matsen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Russel et al. study and reveal compelling evidence for potential sequence-based factors that may drive VDJ trimming, a mechanism involved in VDJ recombination that shapes adaptive immune repertoire generation. The work is based on a rigorous statistical comparison of logistic regression models to reveal the role and function of cutting enzymes in shaping T- and B-cell receptor diversity. It could provide fundamental new insights into these processes with some claims being currently incomplete.

   Reviewed by eLife

7. Spatiotemporal neural dynamics of object recognition under uncertainty in humans

   This article has 3 authors:

   1. Yuan-hao Wu
   2. Ella Podvalny
   3. Biyu J He

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study investigates the spatiotemporal characteristics of human brain activities during object recognition under noisy and ambiguous conditions. By using state-of-the-art data analysis and model-driven fusion of MEG and 7T, this work demonstrates distinct representational profiles in ventral and dorsal pathways, contributing new perspectives to our understanding of the neural implementation of object recognition under uncertainty.

   Reviewed by eLife

8. Actin-regulated Siglec-1 nanoclustering influences HIV-1 capture and virus-containing compartment formation in dendritic cells

   This article has 12 authors:

   1. Enric Gutiérrez-Martínez
   2. Susana Benet Garrabé
   3. Nicolas Mateos
   4. Itziar Erkizia
   5. Jon Ander Nieto-Garai
   6. Maier Lorizate
   7. Kyra JE Borgman
   8. Carlo Manzo
   9. Felix Campelo
   10. Nuria Izquierdo-Useros
   11. Javier Martinez-Picado
   12. Maria F Garcia-Parajo

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Siglec-1 (CD169), a plasma membrane-associated sialic acid-binding lectin, has been implicated in the capture of HIV and other viruses by dendritic cells and macrophages. However, the molecular details of how HIV particles are captured by Siglec-1 are poorly understood. In this paper, the authors use advanced imaging methods to analyse the cell surface distribution of Siglec-1 on immature and mature dendritic cells to study the regulation of Siglec-1 distribution by actin and regulators of actin polymerization and to understand how virus-Siglec-1 engagement leads to virus sequestration within so-called virus containing compartments. These types of analyses have only recently become feasible with the implementation of super-resolution imaging and as yet few virus-host cell systems have been examined in detail. Thus, this study has relevance for researchers studying the engagement of HIV and many other viruses with cells, as well as researchers interested in the mechanisms regulating receptor distribution and function on cells.

   Reviewed by eLife

9. Binding and sequestration of poison frog alkaloids by a plasma globulin

   This article has 10 authors:

   1. Aurora Alvarez-Buylla
   2. Marie-Therese Fischer
   3. Maria Dolores Moya Garzon
   4. Alexandra E Rangel
   5. Elicio E Tapia
   6. Julia T Tanzo
   7. H Tom Soh
   8. Luis A Coloma
   9. Jonathan Z Long
   10. Lauren A O'Connell

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Poison frogs sequester alkaloids to make themselves toxic or unpalatable to predators, but how this sequestration occurs is not well understood. This valuable study identifies an alkaloid-binding protein in the plasma of poison frogs, which may help explain how these animals are able to sequester a diversity of alkaloids with different target sites. The supporting evidence is solid and the study adds to our understanding of how toxic animals resist the effects of their own defenses.

   Reviewed by eLife, Arcadia Science

10. CCR1 mediates Müller cell activation and photoreceptor cell death in macular and retinal degeneration

    This article has 5 authors:

    1. Sarah Elbaz-Hayoun
    2. Batya Rinsky
    3. Shira Hagbi-Levi
    4. Michelle Grunin
    5. Itay Chowers

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      Elbaz-Hayoun et al. investigate the role of macrophages in the gliotic response of retinal Müller glia and photoreceptor cell death. The authors find that macrophages play a role in inducing retinal damage. A role for the muller glia expressed, C-C chemokine receptor axis was identified as a causative factor in promoting retinal degeneration. These important data identify a new link between cells of the immune system and those within the retina which contribute to the progression of retinal degeneration.

    Reviewed by eLife