eLife

Latest preprint reviews

1. Linking genotypic and phenotypic changes in the E. coli long-term evolution experiment using metabolomics

   This article has 6 authors:

   1. John S Favate
   2. Kyle S Skalenko
   3. Eric Chiles
   4. Xiaoyang Su
   5. Srujana Samhita Yadavalli
   6. Premal Shah

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents convincing evidence that metabolite levels in Escherichia coli bacteria from a long-term evolution experiment have changed in consistent ways, which in turn can be explained by recurrent mutations in regulatory genes that affect enzyme expression levels. The use of high-resolution mass spectrometry measuring bulk metabolite levels, in combination with existing gene expression and DNA sequencing datasets provides valuable information linking changes in an organism's genome, transcriptome, and metabolome.

   Reviewed by eLife

2. A tRNA modification in Mycobacterium tuberculosis facilitates optimal intracellular growth

   This article has 4 authors:

   1. Francesca G Tomasi
   2. Satoshi Kimura
   3. Eric J Rubin
   4. Matthew K Waldor

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a valuable addition to the literature as it helps us understand the role of tRNA modifying enzymes in Mycobacterium tuberculosis. By knocking out one of the enzymes, the authors convincingly demonstrate the importance of tRNA-modifying enzymes for intra-host growth of tubercle bacteria. Some of the claims regarding modification as well as the role in virulence could be strengthened through further bioinformatics and phylogenetic analyses as well as experimental approaches. The work will be of interest to microbiologists.

   Reviewed by eLife

3. We couldn't get details of this article at this time:
   10.1101/2023.02.22.529599

4. We couldn't get details of this article at this time:
   10.1101/2023.02.27.530236

5. We couldn't get details of this article at this time:
   10.1101/2023.02.01.526734

6. A toxin-mediated policing system in Bacillus optimizes division of labor via penalizing cheater-like nonproducers

   This article has 11 authors:

   1. Rong Huang
   2. Jiahui Shao
   3. Zhihui Xu
   4. Yuqi Chen
   5. Yunpeng Liu
   6. Dandan Wang
   7. Haichao Feng
   8. Weibing Xun
   9. Qirong Shen
   10. Nan Zhang
   11. Ruifu Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript reports important findings regarding the potential for self-policing and a division of labor among biofilm-inhabiting Bacillus cells. Overall, this work is robust in its use of various techniques and provides solid insights into the intersections of well-understood regulatory controls and the suppression of cheaters. Despite some concerns about the data, all reviewers were excited by the potential impact of this work. Colleagues interested in microbial social interactions should find this study's narrative about the internal mediation of cell differentiation particularly valuable.

   Reviewed by eLife

7. Dissecting the chain of information processing and its interplay with neurochemicals and fluid intelligence across development

   This article has 4 authors:

   1. George Zacharopoulos
   2. Francesco Sella
   3. Uzay Emir
   4. Roi Cohen Kadosh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study combines behavioral and imaging experiments to understand how levels of important brain chemicals shape the processing of information in the brain in children and young adults. The sample size and data quality are outstanding and some of the data are quite convincing. However, the calculation and interpretation of the brain chemical concentration measurements as well as the interpretation of the model-based behavioral parameters are not fully justified and support for the overall conclusions is incomplete. This work will be of interest to neuroscientists, psychologists, and neuroimaging researchers investigating the developing brain in health and disease.

   Reviewed by eLife

8. A TRAF-like E3 ubiquitin ligase TrafE coordinates ESCRT and autophagy in endolysosomal damage response and cell-autonomous immunity to Mycobacterium marinum

   This article has 4 authors:

   1. Lyudmil Raykov
   2. Manon Mottet
   3. Jahn Nitschke
   4. Thierry Soldati

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents important findings on the mechanism as to how Mycobacterium-containing vacuoles are recognized by host cell factors and subjected to membrane repair or autophagic degradation using Dictyostelium discoideum as a useful model. The evidence for the role of TrafE in damaged-membrane repair and xenophagy induction is convincing, but that in autophagosome closure is rather incomplete.

   Reviewed by eLife

9. Motor cortex analogue neurons in songbirds utilize Kv3 channels to generate ultranarrow spikes

   This article has 8 authors:

   1. Benjamin M Zemel
   2. Alexander A Nevue
   3. Leonardo ES Tavares
   4. Andre Dagostin
   5. Peter V Lovell
   6. Dezhe Z Jin
   7. Claudio V Mello
   8. Henrique von Gersdorff

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment:

     Zemel and colleagues provide a report on the fundamental electrophysiological properties of motor neurons driving song in the zebrafish and provide complementary information about cell morphology, pharmacological sensitivity, and ion channel expression and heterogeneity. They provide mainly convincing data supporting the claim of a particular ion channel class, Kv3, that plays an important role in fast electrical spiking (action potentials) in song-related neurons.

   Reviewed by eLife

10. Rapid and precise genome engineering in a naturally short-lived vertebrate

    This article has 5 authors:

    1. Claire N Bedbrook
    2. Ravi D Nath
    3. Rahul Nagvekar
    4. Karl Deisseroth
    5. Anne Brunet

    This article has been curated by 1 group:

    • Curated by eLife

      **eLife assessment
      **
      Within this paper, the authors describe a rapid and easy-to-implement CRISPR/Cas9-mediated knock-in approach to precisely insert large transgenes in the African turquoise killifish. The established method will be instrumental for many researchers working with unusual model species, and, in particular, will expand the killifish community toolbox. It will revolutionize the field and bring the killifish, an emerging animal model in aging biology and disease modeling in vertebrates, into the spotlight even more.

    Reviewed by eLife