eLife

Latest preprint reviews

1. A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation

   This article has 9 authors:

   1. Huaming Xu
   2. Zhijian Li
   3. Chao-Chung Kuo
   4. Katrin Götz
   5. Thomas Look
   6. Marcelo AS de Toledo
   7. Kristin Seré
   8. Ivan G Costa
   9. Martin Zenke

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Authors provide valuable evidence identifying a lncRNA transcribed specifically in the pDC subtype from the +32Kb promoter region which is also the region for the enhancer for Irf8 specifically in the cDC1 subtype. With convincing methodology, they provide in-depth analysis about the possible role of lncIrf8, and its promoter region and cross-talk with Irf8 promoter to identify that it is not the lncIRF8 itself but its promoter region that is crucial for pDC and cDC1 differentiation conferring feedback inhibition of Irf8 transcription. The work will be of interest to immunologists working on immune cell development.

   Reviewed by eLife

2. Impaired iron recycling from erythrocytes is an early hallmark of aging

   This article has 17 authors:

   1. Patryk Slusarczyk
   2. Pratik Kumar Mandal
   3. Gabriela Zurawska
   4. Marta Niklewicz
   5. Komal Chouhan
   6. Raghunandan Mahadeva
   7. Aneta Jończy
   8. Matylda Macias
   9. Aleksandra Szybinska
   10. Magdalena Cybulska-Lubak
   11. Olga Krawczyk
   12. Sylwia Herman
   13. Michal Mikula
   14. Remigiusz Serwa
   15. Małgorzata Lenartowicz
   16. Wojciech Pokrzywa
   17. Katarzyna Mleczko-Sanecka

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Slusarczyk et al demonstrate that red pulp macrophages (RPM), specialized splenic cells that clear senescent red blood cells through erythrophagocytosis, show diminished function in aging mice. This impairment leads to retention of hemolytic red blood cells and formation of extracellular aggregates which further exacerbate RPM demise. Iron restriction alleviates most of these symptoms in aging RPMs. They propose RPM collapse as an early indicator of aging that could be reversed through iron limitation.

   Reviewed by eLife

3. The entorhinal-DG/CA3 pathway in the medial temporal lobe retains visual working memory of a simple surface feature

   This article has 6 authors:

   1. Weizhen Xie
   2. Marcus Cappiello
   3. Michael A Yassa
   4. Edward Ester
   5. Kareem A Zaghloul
   6. Weiwei Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This useful study highlights the contribution of the medial temporal lobe (MTL), and the DG/CA3 hippocampal pathway in particular, to neural activity during the working memory delay period. The evidence supporting this is compelling, using diverse state-of-the-art approaches to neural data analysis and relating it to behavioural data. The work will be of significant interest to neuroscientists specialising in the research area of human working memory.

   Reviewed by eLife

4. B cell receptor-induced IL-10 production from neonatal mouse CD19+CD43- cells depends on STAT5-mediated IL-6 secretion

   This article has 5 authors:

   1. Jiro Sakai
   2. Jiyeon Yang
   3. Chao-Kai Chou
   4. Wells W Wu
   5. Mustafa Akkoyunlu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors present valuable findings about the mechanisms inducing IL-10 production by B cells in neonates. The evidence supporting the claims of the authors is solid, although the manuscript would be strengthened by amendments to the presentation of the data and explanation of some experimental choices.

   Reviewed by eLife

5. Protein composition of axonal dopamine release sites in the striatum

   This article has 3 authors:

   1. Lauren Kershberg
   2. Aditi Banerjee
   3. Pascal S Kaeser

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Using in-vivo proximity labeling using three different presynaptic proteins the authors have carried out a comprehensive proteome analysis of axonal dopamine release sites, resulting in the identification of many new presynaptic candidate proteins. Genetic deletion of the active zone protein RIM1 but not of the presynaptic calcium sensor synaptotagmin 1 resulted in a loss of enrichment indicative of a disruption of the active zone. Although the functional significance of many of the novel proteins will require future corroboration, the analysis provides a valuable and high-quality dataset as a starting point for future investigations.

   Reviewed by eLife

6. Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells

   This article has 13 authors:

   1. Yasunari Seita
   2. Keren Cheng
   3. John R McCarrey
   4. Nomesh Yadu
   5. Ian H Cheeseman
   6. Alec Bagwell
   7. Corinna N Ross
   8. Isamar Santana Toro
   9. Li-hua Yen
   10. Sean Vargas
   11. Christopher S Navara
   12. Brian P Hermann
   13. Kotaro Sasaki

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper describes a method for robust differentiation of the common marmoset induced pluripotent stem cells (iPSCs) into primordial germ cell-like cells and subsequently into spermatogonia-like cells when combined with testis somatic cells. The data suggest that marmosets are very similar to humans and macaques. The paper is nicely done but needs further characterization and a better explanation of the methodology.

   Reviewed by eLife

7. Evolved bacterial resistance to the chemotherapy gemcitabine modulates its efficacy in co-cultured cancer cells

   This article has 8 authors:

   1. Serkan Sayin
   2. Brittany Rosener
   3. Carmen G Li
   4. Bao Ho
   5. Olga Ponomarova
   6. Doyle V Ward
   7. Albertha JM Walhout
   8. Amir Mitchell

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental work advances our understanding of how bacteria evolve to resist drugs used for cancer treatment and how this could potentially affect drug efficacy and treatment outcome. The data were collected and analyzed using a solid methodology and can be used as a starting point for functional studies of the interaction between microbiome interactions and cancer drug treatment. The findings will be of broad interest to microbiologists and organismal biologists interested in the role of microbiomes in drug responses.

   Reviewed by eLife

8. Mechanical basis and topological routes to cell elimination

   This article has 4 authors:

   1. Siavash Monfared
   2. Guruswami Ravichandran
   3. José Andrade
   4. Amin Doostmohammadi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this work, Monfared et al. construct a useful three-dimensional phase-field model for cell layers and use this to investigate the link of extrusion events to defects in cellular arrangement. The extension of existing 2D phase field models to three dimensions is an important contribution of this paper. Here the model is used to study the importance of cell-cell and cell-substrate interaction in extrusion from cell monolayers. Their claim that extrusion events can be distinctly linked to defects in nematic and hexatic orders in the monolayer need to be better justified to be fully convincing.

   Reviewed by eLife

9. Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation

   This article has 9 authors:

   1. Eshwar R Tammineni
   2. Lourdes Figueroa
   3. Carlo Manno
   4. Disha Varma
   5. Natalia Kraeva
   6. Carlos A Ibarra
   7. Amira Klip
   8. Sheila Riazi
   9. Eduardo Rios

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Junctophilin has been traditionally known as a structural anchor to keep excitation-contraction proteins in place for healthy contractile function of skeletal muscle. Here the authors provide an interesting and important role in skeletal muscle for Junctophilin, where it translocates to the nuclei and influences gene transcription. The authors provide convincing evidence for a novel role of junctophilin beyond its structural role as a regulator of gene expression.

   Reviewed by eLife

10. T follicular helper 17 (Tfh17) cells are superior for immunological memory maintenance

    This article has 28 authors:

    1. Xin Gao
    2. Kaiming Luo
    3. Diya Wang
    4. Yunbo Wei
    5. Yin Yao
    6. Jun Deng
    7. Yang Yang
    8. Qunxiong Zeng
    9. Xiaoru Dong
    10. Le Xiong
    11. Dongcheng Gong
    12. Lin Lin
    13. Kai Pohl
    14. Shaoling Liu
    15. Yu Liu
    16. Lu Liu
    17. Thi HO Nguyen
    18. Lilith F Allen
    19. Katherine Kedzierska
    20. Yanliang Jin
    21. Mei-Rong Du
    22. Wanping Chen
    23. Liangjing Lu
    24. Nan Shen
    25. Zheng Liu
    26. Ian A Cockburn
    27. Wenjing Luo
    28. Di Yu

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      The enrichment of Tfh17 cells in Tfh cell central memory compartment and the dominance of Tfh17 cell population and the Tfh17 transcriptional signature in circulating Tfh cells at the memory phase are nicely demonstrated, and may well be helpful for understanding the heterogeneity of memory Tfh cells and potentially providing clues for vaccine design. The in vitro differentiation system for mouse Tfh cells also provides a strategy for others to build upon in dissection of Tfh cell development and function.

    Reviewed by eLife