eLife

Latest preprint reviews

1. Design principles for inflammasome inhibition by pyrin-only-proteins

   This article has 10 authors:

   1. Shuai Wu
   2. Archit Garg
   3. Zachary Mazanek
   4. Gretchen Belotte
   5. Jeffery J Zhou
   6. Christina M Stallings
   7. Jacob Lueck
   8. Aubrey Roland
   9. Michael A Chattergoon
   10. Jungsan Sohn

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this useful and potentially important manuscript, Mazanek and colleagues combine computational analysis and in vitro experiments to develop a comprehensive analysis of the ability of pyrin-only proteins (POPs) to inhibit inflammasome assembly. The results lead the authors to propose that a mixture of favorable and unfavorable interaction surfaces is required for a POP to inhibit a given inflammasome component. The results presented are solid, but additional experimentation is required to fully justify the authors' model.

   Reviewed by eLife

2. Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells

   This article has 3 authors:

   1. Andrea McQuate
   2. Sharmon Knecht
   3. David W Raible

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Mutations in mitochondrial genes can lead to deafness but the mitochondrial biology of sensory hair cells is not well understood. In this study, high-resolution imaging of mitochondrial development in sensory hair cells of normal and mutant zebrafish lateral line systems was described. The authors provide evidence that the formation of the mitochondrial architecture requires normal hair cell activity. This paper is of potential interest to researchers interested in metabolic homeostasis and sensory hair cell biology.

   Reviewed by eLife

3. Cryo-plasma FIB/SEM volume imaging of biological specimens

   This article has 20 authors:

   1. Maud Dumoux
   2. Thomas Glen
   3. Jake LR Smith
   4. Elaine ML Ho
   5. Luis MA Perdigão
   6. Avery Pennington
   7. Sven Klumpe
   8. Neville BY Yee
   9. David Andrew Farmer
   10. Pui YA Lai
   11. William Bowles
   12. Ron Kelley
   13. Jürgen M Plitzko
   14. Liang Wu
   15. Mark Basham
   16. Daniel K Clare
   17. C Alistair Siebert
   18. Michele C Darrow
   19. James H Naismith
   20. Michael Grange

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important work presented is of interest to the electron microscopy community, which expanding to more and more cell biologists. The field has long searched for a suitable method to combine the pristine preservation of vitrified samples with a volumetric imaging modality that reveals subcellular architecture at sufficient contrast for ultrastructural analyses, and the authors describe here the use of novel ion beams for imaging cellular samples in three dimensions, concluding that one of the four plasma sources tested produces the highest quality images. This allows them to provide several recommendations for imaging along with software for improving collected images. This approach should be very useful for addressing many biological questions.

   Reviewed by eLife

4. The long noncoding RNA Charme supervises cardiomyocyte maturation by controlling cell differentiation programs in the developing heart

   This article has 15 authors:

   1. Valeria Taliani
   2. Giulia Buonaiuto
   3. Fabio Desideri
   4. Adriano Setti
   5. Tiziana Santini
   6. Silvia Galfrè
   7. Leonardo Schirone
   8. Davide Mariani
   9. Giacomo Frati
   10. Valentina Valenti
   11. Sebastiano Sciarretta
   12. Emerald Perlas
   13. Carmine Nicoletti
   14. Antonio Musarò
   15. Monica Ballarino

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript reports important and valuable new data about the intriguing role of the lncRNA Charme during cardiac development. Whilst the majority of claims are convincingly supported by the data, the evidence for the cardiac phenotype and the mechanism by which Charme/MATR3 interacts is currently incomplete and requires additional experimental support. This paper is of general interest to cardiac developmental biologists as well as to anyone studying non-coding RNAs.

   Reviewed by eLife

5. Plasmodium falciparum adapts its investment into replication versus transmission according to the host environment

   This article has 17 authors:

   1. Abdirahman I Abdi
   2. Fiona Achcar
   3. Lauriane Sollelis
   4. João Luiz Silva-Filho
   5. Kioko Mwikali
   6. Michelle Muthui
   7. Shaban Mwangi
   8. Hannah W Kimingi
   9. Benedict Orindi
   10. Cheryl Andisi Kivisi
   11. Manon Alkema
   12. Amrita Chandrasekar
   13. Peter C Bull
   14. Philip Bejon
   15. Katarzyna Modrzynska
   16. Teun Bousema
   17. Matthias Marti

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable work provides insight into how Plasmodium falciparum optimises the balance between infection of the human host and investment in onward transmission to the mosquito. Based on the appropriate and validated methodology most of the results are convincing, nonetheless, some conclusions are incomplete and require further support.

   Reviewed by eLife

6. The lingering effects of Neanderthal introgression on human complex traits

   This article has 10 authors:

   1. Xinzhu Wei
   2. Christopher R Robles
   3. Ali Pazokitoroudi
   4. Andrea Ganna
   5. Alexander Gusev
   6. Arun Durvasula
   7. Steven Gazal
   8. Po-Ru Loh
   9. David Reich
   10. Sriram Sankararaman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     A small proportion of the genomes of humans whose ancestors lived outside Africa traces back to an interbreeding event with Neanderthals. While we know that selection has generally acted to remove Neanderthal ancestry, intense interest has focused on understanding the contribution to current human phenotypic variation. This paper uses a new set of approaches to carefully quantify this contribution, taking into account various complicating factors. The work will be of interest to colleagues in human evolution and evolutionary biology more generally.

   Reviewed by eLife

7. Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis

   This article has 19 authors:

   1. Emma A Hall
   2. Dhivya Kumar
   3. Suzanna L Prosser
   4. Patricia L Yeyati
   5. Vicente Herranz-Pérez
   6. Jose Manuel García-Verdugo
   7. Lorraine Rose
   8. Lisa McKie
   9. Daniel O Dodd
   10. Peter A Tennant
   11. Roly Megaw
   12. Laura C Murphy
   13. Marisa F Ferreira
   14. Graeme Grimes
   15. Lucy Williams
   16. Tooba Quidwai
   17. Laurence Pelletier
   18. Jeremy F Reiter
   19. Pleasantine Mill

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript will be of interest to centrosome and cilia cell biologists. It evaluates the in vivo and in vitro role of PCM1, and by extension, centriole satellites in ciliogenesis. The major strength of this study is the detailed characterisation of Pcm1-/- mutant mice, which reveals a role for PCM1 in biogenesis of specific types of cilia, such as motile cilia on ependymal cells. The claims are generally well supported by the data, but the mechanistic basis for the cell-type specific requirement for PCM1 in ciliogenesis remains to be fully clarified.

   Reviewed by eLife

8. Neural circuits underlying habituation of visually evoked escape behaviors in larval zebrafish

   This article has 2 authors:

   1. Haleh Fotowat
   2. Florian Engert

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In the present study, the authors discovered the inhibitory neurons that are potentiated during the repetitive visual stimuli and control neurons that transmit looming information to evoke escape responses. Thus, the study elucidated a principle of habituation using a model vertebrate zebrafish.

   Reviewed by eLife

9. HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation

   This article has 15 authors:

   1. Sara Scinicariello
   2. Adrian Soderholm
   3. Markus Schäfer
   4. Alexandra Shulkina
   5. Irene Schwartz
   6. Kathrin Hacker
   7. Rebeca Gogova
   8. Robert Kalis
   9. Kimon Froussios
   10. Valentina Budroni
   11. Annika Bestehorn
   12. Tim Clausen
   13. Pavel Kovarik
   14. Johannes Zuber
   15. Gijs A Versteeg

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The RNA-binding protein Tristetraprolin (TPP) regulates the abundance of mRNAs encoding proinflammatory cytokines. The study by Scinicariello and collaborators examined mechanisms regulating the turnover of TTP in cultured cells and identified the ubiquitin E3 ligase HUWE1 as a regulator of TPP degradation. The conclusions are largely supported by the cellular and biochemical experiments. This paper thus implicates the HUWE1-TPP axis in regulating macrophage inflammatory responses at the post-transcriptional steps.

   Reviewed by eLife

10. Nitrogenase resurrection and the evolution of a singular enzymatic mechanism

    This article has 7 authors:

    1. Amanda K Garcia
    2. Derek F Harris
    3. Alex J Rivier
    4. Brooke M Carruthers
    5. Azul Pinochet-Barros
    6. Lance C Seefeldt
    7. Betül Kaçar

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This manuscript reports valuable findings regarding the evolution of nitrogenases through ancestral sequence reconstruction and resurrection. The results are solid and support the conclusions of the study, and highlight the historical constraints that have been acting on this enzyme. The findings will be of interest for people interested in enzyme evolution in general and particularly for those interested in the evolution of nitrogenases.

    Reviewed by eLife