eLife

Latest preprint reviews

1. Cell chirality reversal through tilted balance between polymerization of radial fibers and clockwise-swirling of transverse arcs

   This article has 12 authors:

   1. Hoi Kwan Kwong
   2. Miu Ling Lam
   3. Siying Wu
   4. Cho Fan Chung
   5. Jianpeng Wu
   6. Lok Ting Chu
   7. King Hoo Lim
   8. Hiu Lam Chow
   9. Hogi Hartanto
   10. Wengang Liu
   11. Kwan Ting Chow
   12. Ting-Hsuan Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The intrinsic chirality of actin filaments (F-actin) is implicated in the chiral arrangement and movement of cellular structures, but it was unknown how opposite chiralities can arise when the chirality of F-actin is invariant. Kwong et al. present evidence that two actin filament-based cytoskeletal structures, transverse actin arcs and radial stress fibers, drive clockwise and anti-clockwise rotation, respectively. This fundamental work, which has broad implications for cell biology, is supported by compelling data.

   Reviewed by eLife

2. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2312.17293v3

3. TRPML1 gating modulation by allosteric mutations and lipids

   This article has 4 authors:

   1. Ninghai Gan
   2. Yan Han
   3. Weizhong Zeng
   4. Youxing Jiang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Transient receptor potential mucolipin 1 (TRPML1) functions as a lysosomal ion channel whose variants are associated with lysosomal storage disorder mucolipidosis type IV. This important report describes local and global structural changes driven by binding of regulatory phospholipids and by mutations that allosterically cause gain or loss of channel function. Most of the claims related to the allosteric regulation of TRPML1 are convincingly supported by two new cryo-EM structures which are evaluated within the context of previously reported TRPML1 structures, and a proposed allosteric gating mechanism is partially supported by functional electrophysiology results.

   Reviewed by eLife

4. The evolutionary modifications of a GoLoco motif in the AGS protein facilitate micromere formation in the sea urchin embryo

   This article has 4 authors:

   1. Natsuko Emura
   2. Florence D.M. Wavreil
   3. Annaliese Fries
   4. Mamiko Yajima

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study presents work on the molecular mechanism driving asymmetric cell division and fate decisions during embryonic development of echinoids. The evidence supporting the claims of the authors is solid overall but with some concerns about quantification and a lack of explanation for some of the findings. The work will be of interest to developmental biologists and cell biologists working in the field of self-renewal.

   Reviewed by eLife

5. Deuterium Metabolic Imaging Phenotypes Mouse Glioblastoma Heterogeneity Through Glucose Turnover Kinetics

   This article has 9 authors:

   1. Rui V Simões
   2. Rafael N Henriques
   3. Jonas L Olesen
   4. Beatriz M Cardoso
   5. Francisca F Fernandes
   6. Mariana AV Monteiro
   7. Sune N Jespersen
   8. Tânia Carvalho
   9. Noam Shemesh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work describes a convincingly validated non-invasive tool for in vivo metabolic phenotyping of aggressive brain tumors in mice brains. The analysis provides a valuable technique that tackles the unmet need for patient stratification and hence for early assessment of therapeutic efficacy. However, wider clinical applicability of the findings can be attained by expanding the work to include more diverse tumor models.

   Reviewed by eLife

6. One N-glycan regulates natural killer cell antibody-dependent cell-mediated cytotoxicity and modulates Fc γ receptor IIIa/CD16a structure

   This article has 4 authors:

   1. Paul G Kremer
   2. Elizabeth A Lampros
   3. Allison M Blocker
   4. Adam W Barb

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study explores the mechanistic link between glycosylation at the N162 site of the Fc gamma receptor FcγRIIIa and the modulation of NK cell-mediated antibody-dependent cytotoxicity. Using innovative isotope labeling strategies and advanced NMR spectroscopy techniques, the authors provide compelling evidence of how glycan composition influences receptor stability and immune function. These findings offer fundamental insights that may contribute to the development of more effective therapeutic antibodies. The manuscript will be of significant interest to immunologists and researchers focused on therapeutic antibody design.

   Reviewed by eLife

7. Allosteric inhibition of trypanosomatid pyruvate kinases by a camelid single-domain antibody

   This article has 14 authors:

   1. Joar Esteban Pinto Torres
   2. Mathieu Claes
   3. Rik Hendrickx
   4. Meng Yuan
   5. Natalia Smiejkowska
   6. Pieter Van Wielendaele
   7. Hans De Winter
   8. Serge Muyldermans
   9. Paul A Michels
   10. Malcolm D Walkinshaw
   11. Wim Versées
   12. Guy Caljon
   13. Stefan Magez
   14. Yann G.-J Sterckx

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work presents valuable data demonstrating that a camelid single-domain antibody can selectively inhibit a key glycolytic enzyme in trypanosomes via an allosteric mechanism. The claim that this information can be exploited for the design of novel chemotherapeutics is incomplete and limited by the modest effects on parasite growth, as well as the lack of evidence for cellular target engagement in vivo.

   Reviewed by eLife

8. MGPfact ^XMBD : A Model-Based Factorization Method for scRNA Data Unveils Bifurcating Transcriptional Modules Underlying Cell Fate Determination

   This article has 9 authors:

   1. Jun Ren
   2. Ying Zhou
   3. Yudi Hu
   4. Jing Yang
   5. Hongkun Fang
   6. Xuejing Lyu
   7. Jintao Guo
   8. Xiaodong Shi
   9. Qiyuan Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript describes a novel computational method to investigate cell evolutionary trajectory for scRNA-seq samples. This is an important tool for estimating pseudotime in the evolutionary path through modelling the bifurcations in a Gaussian process. While the evaluation of the method is extensive and compelling, the reviewers suggested further analyses to ensure that the method is indeed robust. When these issues are addressed, this will be of substantive value to biologists interested in scRNA-seq bioinformatic methods.

   Reviewed by eLife

9. Redistribution of fragmented mitochondria ensure symmetric organelle partitioning and faithful chromosome segregation in mitotic mouse zygotes

   This article has 11 authors:

   1. Haruna Gekko
   2. Ruri Nomura
   3. Daiki Kuzuhara
   4. Masato Kaneyasu
   5. Genpei Koseki
   6. Deepak Adhikari
   7. Yasuyuki Mio
   8. John Carroll
   9. Tomohiro Kono
   10. Hiroaki Funahashi
   11. Takuya Wakai

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study investigates the role of Drp1 in early embryo development, providing solid evidence on how this protein influences mitochondrial localization and partitioning during the first embryonic divisions. The research employs the Trim-Away technique to eliminate Drp1 in zygotes, revealing critical insights into mitochondrial clustering, spindle formation, and embryonic development.

   Reviewed by eLife

10. H2A.Z deposition at meiotic prophase I underlies homologous recombination and pachytene genome activation during male meiosis

    This article has 18 authors:

    1. Shenfei Sun
    2. Yamei Jiang
    3. Ning Jiang
    4. Qiaoli Zhang
    5. Hongjie Pan
    6. Fujing Huang
    7. Xinna Zhang
    8. Yuxuan Guo
    9. Xiaoyu You
    10. Kai Gong
    11. Wei Wei
    12. Hanmin Liu
    13. Zhenju Song
    14. Yuanlin Song
    15. Xiaofang Tang
    16. Miao Yu
    17. Runsheng Li
    18. Xinhua Lin

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This study shows that Znhit1, a regulator of chromatin and of the histone variant H2A.Z, is required for progression through meiotic prophase. It is an important observation that describes the role of epigenetics and gene expression during meiosis. The analysis is based on complementary approaches at the cytological, single-cell, and genomic levels that provide solid evidence for the role of Znhit1 in the control of gene expression and in the loading of H2A.Z in mouse spermatocytes.

    Reviewed by eLife