eLife

Latest preprint reviews

1. Interplay between acetylation and ubiquitination of imitation switch chromatin remodeler Isw1 confers multidrug resistance in Cryptococcus neoformans

   This article has 15 authors:

   1. Yang Meng
   2. Zhuoran Li
   3. Tianhang Jiang
   4. Tianshu Sun
   5. Yanjian Li
   6. Xindi Gao
   7. Hailong Li
   8. Chenhao Suo
   9. Chao Li
   10. Sheng Yang
   11. Tian Lan
   12. Guojian Liao
   13. Tong-Bao Liu
   14. Ping Wang
   15. Chen Ding

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study links chromatin remodeling with antifungal drug resistance in Cryptococcus neoformans. The work is important because it reveals a new facet of how drug resistance can emerge and associates. The work presented is well done but the story is incomplete since there are questions about methods and association that need to be addressed. Establishing a link between chromatin remodeling and antifungal resistance is a finding that would be of interest to infectious disease researchers, cell biologists, and drug developers.

   Reviewed by eLife

2. Roles for mycobacterial DinB2 in frameshift and substitution mutagenesis

   This article has 7 authors:

   1. Pierre Dupuy
   2. Shreya Ghosh
   3. Allison Fay
   4. Oyindamola Adefisayo
   5. Richa Gupta
   6. Stewart Shuman
   7. Michael S Glickman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study uses a combination of compelling biochemical and genetic approaches to identify a highly mutagenic DNA polymerase, which drives a wide spectrum of mutations when overexpressed. The important findings advance the understanding of mutagenesis in mycobacteria. The work will be of interest to bacteriologists interested in mutagenesis and the emergence of drug resistance.

   Reviewed by eLife

3. Three-dimensional imaging of vascular development in the mouse epididymis

   This article has 16 authors:

   1. Christelle Damon-Soubeyrand
   2. Antonino Bongiovanni
   3. Areski Chorfa
   4. Chantal Goubely
   5. Nelly Pirot
   6. Luc Pardanaud
   7. Laurence Piboin-Fragner
   8. Caroline Vachias
   9. Stephanie Bravard
   10. Rachel Guiton
   11. Jean-Leon Thomas
   12. Fabrice Saez
   13. Ayhan Kocer
   14. Meryem Tardivel
   15. Joël R Drevet
   16. Joelle Henry-Berger

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     There are many strengths in this paper that examines patterns of epididymal blood and lymphatic vasculature, supported by quantitative methods, and well-conducted 3D imaging studies (graphics and videos). Minor weaknesses include the lack of higher magnification images and the organization of image panels in some figures. Overall, this is a very important contribution to the epididymis research field.

   Reviewed by eLife

4. Limited role of generation time changes in driving the evolution of the mutation spectrum in humans

   This article has 5 authors:

   1. Ziyue Gao
   2. Yulin Zhang
   3. Nathan Cramer
   4. Molly Przeworski
   5. Priya Moorjani

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study, of interest to population geneticists and evolutionary biologists alike, aims at investigating temporal variation in patterns of germline mutation during the evolution of human populations. The authors suggest that shifts in mutation spectra occur frequently, over a few thousands of generations, possibly as a consequence of changes in environmental exposure, or of genetic modifiers. There are several important aspects of methodology that need to be clarified, and several additional tests have to be done to confirm that the reported observations are not the result of methodological artifacts. The paper also overstates certain weaknesses of previously published papers on mutation spectrum evolution as well as the generation time hypothesis; correcting these oversimplifications would more accurately capture what the paper's new analyses add to the state of knowledge in these areas.

   Reviewed by eLife

5. Transferred mitochondria accumulate reactive oxygen species, promoting proliferation

   This article has 14 authors:

   1. Chelsea U Kidwell
   2. Joseph R Casalini
   3. Soorya Pradeep
   4. Sandra D Scherer
   5. Daniel Greiner
   6. Defne Bayik
   7. Dionysios C Watson
   8. Gregory S Olson
   9. Justin D Lathia
   10. Jarrod S Johnson
   11. Jared Rutter
   12. Alana L Welm
   13. Thomas A Zangle
   14. Minna Roh-Johnson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript provides compelling evidence that macrophages transfer mitochondria to cancer cells and that transferred mitochondria stimulate proliferation in recipient cells. The usage an array of clever cell biology-based tools provides compelling evidence for these claims despite the difficulties associated with studying a relatively low probability event. Solid evidence supports the proposed model that transferred mitochondria induce proliferation by stimulating ERK signaling in a ROS dependent manner, although at present some aspects of the proposed model are incomplete. The work has broad significance for both mitochondrial biology and cancer biology as the authors show clear evidence of mitochondrial transfer in mouse models of human tumors.

   Reviewed by eLife, ASAPbio crowd review

6. Opioid suppression of an excitatory pontomedullary respiratory circuit by convergent mechanisms

   This article has 2 authors:

   1. Jordan T Bateman
   2. Erica S Levitt

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Opioid-induced respiratory depression is one of the side effects of opioid drugs. Although opioid overdose deaths are highly prevalent, our knowledge of the neural circuits underlying respiratory depression in the brainstem is far from complete. The present study used a variety of sophisticated experimental techniques to convincingly reveal the identity of brainstem components that are part of the neural circuits involved in the mediation of opioid respiratory effects, together with defining potential synaptic underlying mechanisms. They focused on two regions of the brainstem, namely the Kolliker-Fuse and the preBötzinger Complex, and proposed a combination of three complementary processes at pre- and post-synaptic sites in both KF and preBötC regions to explain respiratory depression linked to opioid exposure. This study provides very important findings on the circuitry involved in opioid-induced respiratory depression, and the present results are of broad interest to the respiratory control research community, as well as medically relevant.

   Reviewed by eLife

7. Intravital imaging-based genetic screen reveals the transcriptional network governing Candida albicans filamentation during mammalian infection

   This article has 4 authors:

   1. Rohan S Wakade
   2. Laura C Ristow
   3. Melanie Wellington
   4. Damian J Krysan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Candida morphogenesis is important for virulence. This study provides important new information as to how C. albicans regulates the switch from budding to hyphal morphology. Their results identify transcription factors involved in the process of hyphal morphogenesis in the host. The results are convincing and will be interesting to scientists in the fields of medical mycology and cell biology.

   Reviewed by eLife

8. Mega-scale movie-fields in the mouse visuo-hippocampal network

   This article has 2 authors:

   1. Chinmay Purandare
   2. Mayank Mehta

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript analyzes large-scale Neuropixels recordings from visual areas and hippocampus of mice passively viewing repeated clips of a movie and reports that neurons respond with elevated firing activities to specific, continuous sequences of movie frames. The important results support a role of rodent hippocampal neurons in general episode encoding and advance understanding of visual information processing across different brain regions. The strength of evidence for the primary conclusion was found to be convincing.

   Reviewed by eLife

9. Arabidopsis transcriptome responses to low water potential using high-throughput plate assays

   This article has 10 authors:

   1. Stephen Gonzalez
   2. Joseph Swift
   3. Adi Yaaran
   4. Jiaying Xu
   5. Charlotte Miller
   6. Natanella Illouz-Eliaz
   7. Joseph R Nery
   8. Wolfgang Busch
   9. Yotam Zait
   10. Joseph R Ecker

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work critically evaluates several widely-used assays of transcriptional responses to water limitation in Arabidopsis grown on defined agar-solidified media and, finding inconsistent responses in root transcriptome responses, introduces a new 'hard agar' assay with more consistent responses. The work is valuable as a simple and alternative experimental system that would enable high-throughput genetic screening (and GWAS) to assess the impacts of environmental perturbations on transcriptional responses in various genetic backgrounds. Within this scope, the work is solid, though the debate about whether field-level physiological inferences can be made from such assays remains.

   Reviewed by eLife

10. A stable, distributed code for cue value in mouse cortex during reward learning

    This article has 5 authors:

    1. David J Ottenheimer
    2. Madelyn M Hjort
    3. Anna J Bowen
    4. Nicholas A Steinmetz
    5. Garret D Stuber

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This study makes valuable observations about the representation of "value" in the mouse brain, by using a nice task design and recording from an impressive number of brain regions. The combination of state-of-the-art imaging and electrophysiology data offer solid support for the authors' conclusions. The paper will be of interest to a broad audience of neuroscientists interested in reward processing in the brain.

    Reviewed by eLife