eLife

Latest preprint reviews

1. TCR-pMHC complex formation triggers CD3 dynamics

   This article has 8 authors:

   1. Floris J. van Eerden
   2. Aalaa Alrahman Sherif
   3. Mara Anais Llamas-Covarrubias
   4. Arthur Millius
   5. Xiuyuan Lu
   6. Shigenari Ishizuka
   7. Sho Yamasaki
   8. Daron M. Standley

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     There was a range of opinion among three highly expert reviewers from different perspectives in the field. This is a significant topic and it was felt that the contribution at present is valuable to those in the field. However, it was agreed after consultation that the description of the simulation methodology was inadequate.

   Reviewed by eLife

2. Chromosome-level genome assembly of hadal snailfish reveals mechanisms of deep-sea adaptation in vertebrates

   This article has 16 authors:

   1. Wenjie Xu
   2. Chenglong Zhu
   3. Xueli Gao
   4. Baosheng Wu
   5. Han Xu
   6. Mingliang Hu
   7. Honghui Zeng
   8. Xiaoni Gan
   9. Chenguang Feng
   10. Jiangmin Zheng
   11. Jing Bo
   12. Li-Sheng He
   13. Qiang Qiu
   14. Wen Wang
   15. Shunping He
   16. Kun Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study advances our understanding of the potential mechanisms of deep-sea adaptation and sheds light on the evolutionary history of hadal snailfish. Through comparative genomic analysis, the authors provide convincing evidence and propose hypotheses on the timing of trench colonization, population structure, and adaptations to the hadal snailfish genome in response to their environment.

   Reviewed by eLife

3. Long-read single-cell sequencing reveals expressions of hypermutation clusters of isoforms in human liver cancer cells

   This article has 10 authors:

   1. Silvia Liu
   2. Yan-Ping Yu
   3. Bao-Guo Ren
   4. Tuval Ben-Yehezkel
   5. Caroline Obert
   6. Mat Smith
   7. Wenjia Wang
   8. Alina Ostrowska
   9. Alejandro Soto-Gutierrez
   10. Jian-Hua Luo

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors pair single-cell sequencing technology with the LoopSeq synthetic long-read method to examine samples of hepatocellular carcinoma and benign liver, with the goal of identifying mutations and fusion transcripts specific to cancer cells. The authors present a valuable resource and the overall support for the major claims is solid.

   Reviewed by eLife

4. Sibling similarity can reveal key insights into genetic architecture

   This article has 4 authors:

   1. Tade Souaiaia
   2. Hei Man Wu
   3. Clive Hoggart
   4. Paul F O'Reilly

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors present a solid statistical framework for using sibling phenotype data to assess whether there is evidence for de-novo or rare variants causing extreme trait values. Their valuable method is promising and will be of interest to researchers studying complex trait genetics.

   Reviewed by eLife

5. Is tumor mutational burden predictive of response to immunotherapy?

   This article has 5 authors:

   1. Carino Gurjao
   2. Dina Tsukrov
   3. Maxim Imakaev
   4. Lovelace J Luquette
   5. Leonid A Mirny

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study examines relationships between tumor mutational burden and the response to immunotherapy, using new data sets along with publicly available data sets. The authors conclude that tumor mutational burden cut-offs are unreliable proxies for predicting the response to therapy, underpinned by solid evidence, but with several caveats and assumptions that leave the central question subject to further inquiry. In summary, this is an interesting study that adds to a growing body of work investigating the particular conditions governing the effectiveness of immunotherapy.

   Reviewed by eLife

6. A new look at the architecture and dynamics of the Hydra nerve net

   This article has 13 authors:

   1. Athina Keramidioti
   2. Sandra Schneid
   3. Christina Busse
   4. Christoph Cramer von Laue
   5. Bianca Bertulat
   6. Willi Salvenmoser
   7. Martin Hess
   8. Olga Alexandrova
   9. Kristine M Glauber
   10. Robert E Steele
   11. Bert Hobmayer
   12. Thomas W Holstein
   13. Charles N David

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work presents important findings on the cellular and ultrastructural organization of the nervous system in the freshwater polyp Hydra. The authors present outstanding imaging data with convincing evidence to support their claims. The manuscript provides a starting point for further functional in vivo studies. The work will be of interest to developmental biologists and neurobiologists.

   Reviewed by eLife

7. Diameter dependence of transport through nuclear pore complex mimics studied using optical nanopores

   This article has 6 authors:

   1. Nils Klughammer
   2. Anders Barth
   3. Maurice Dekker
   4. Alessio Fragasso
   5. Patrick R Onck
   6. Cees Dekker

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study reports on a new method for the fabrication and the analysis of the transport through nuclear pore complexes mimic. Methods, data and analyses are convincing and show a clear correlation between the size of the nuclear pore complex mimic and its transport selectivity. This work will be of high interest to biologists and biophysicists working on the mechanosensitivity of nucleocytoplasmic transport.

   Reviewed by eLife

8. Alternative paths to immune activation: the role of costimulatory risk genes for polygenic inflammatory disease in T helper cells

   This article has 6 authors:

   1. Alexandru-Ioan Voda
   2. Kristina Correa
   3. Jonathan Hamp
   4. Chloe Moscrop
   5. Michael Dustin
   6. Luke Jostins-Dean

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study examines the effects of different co-stimulatory pathways on gene expression and chromatin accessibility in T cells, in order to link the role of co-stimulatory proteins to genetic variants associated with inflammatory bowl disease (IBD). The work provides valuable insight into the role of alternative co-stimulatory proteins in controlling T cell responses. However, the data presented and the analyses performed are still incomplete and inconclusive with regards to IBD risk factors.

   Reviewed by eLife

9. A MSTNDel73C mutation with FGF5 knockout sheep by CRISPR/Cas9 promotes skeletal muscle myofiber hyperplasia

   This article has 21 authors:

   1. Ming-Ming Chen
   2. Yue Zhao
   3. Kun Yu
   4. Xue-Ling Xu
   5. Xiao-Sheng Zhang
   6. Jin-Long Zhang
   7. Su-Jun Wu
   8. Zhi-Mei Liu
   9. Yi-Ming Yuan
   10. Xiao-Fei Guo
   11. Shi-Yu Qi
   12. Guang Yi
   13. Shu-Qi Wang
   14. Huang-Xiang Li
   15. Ao-Wu Wu
   16. Guo-Shi Liu
   17. Shou-Long Deng
   18. Hong-Bing Han
   19. Feng-Hua Lv
   20. Di Lian
   21. Zheng-Xing Lian

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors present a useful analysis of the phenotype of sheep in which the muscle developmental regulator myostatin has been mutated in a FGF5 knockout background. The goal was to produce sheep with a "double-muscled" phenotype, yet the genetically engineered sheep exhibited meat with a smaller cross-sectional area and higher number of muscle fibers. The work extends the extensive body of knowledge already published in this area. The authors provide evidence using in vitro experiments that Fosl1 regulates myogenesis, but the strength of evidence relating to the muscle phenotype and underlying cellular and molecular mechanism remains incomplete.

   Reviewed by eLife

10. Vaccination with mycobacterial lipid loaded nanoparticle leads to lipid antigen persistence and memory differentiation of antigen-specific T cells

    This article has 12 authors:

    1. Eva Morgun
    2. Jennifer Zhu
    3. Sultan Almunif
    4. Sharan Bobbala
    5. Melissa S Aguilar
    6. Junzhong Wang
    7. Kathleen Conner
    8. Yongyong Cui
    9. Liang Cao
    10. Chetan Seshadri
    11. Evan A Scott
    12. Chyung-Ru Wang

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      The authors generate a new formulation built upon a previous nanoparticle platform to generate a new system termed bicontinuous nanospheres (BCN), allowing for the dual incorporation of lipid and protein antigens. The authors generate mycolic acid (MA)-loaded BCN and perform a series of characterization studies to demonstrate the superior performance of this new formulation relative to the original one in terms of antigen persistence, a quality needed to sustain responses after vaccination. This work provides important new insights relevant to the TB vaccine field and it suggests that alternative antigens to proteins could be used in TB vaccine formulations. The data are convincing and will be of interest to individuals working on tuberculosis, vaccines and basic immunology.

    Reviewed by eLife