eLife

Latest preprint reviews

1. POMC neurons control fertility through differential signaling of MC4R in Kisspeptin neurons

   This article has 12 authors:

   1. Rajae Talbi
   2. Todd L Stincic
   3. Kaitlin Ferrari
   4. Choi Ji Hae
   5. Karol Walec
   6. Elizabeth Medve
   7. Achi Gerutshang
   8. Silvia León
   9. Elizabeth A McCarthy
   10. Oline K Rønnekleiv
   11. Martin J Kelly
   12. Víctor M Navarro

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The study presents compelling evidence that the melanocortin system originating in the arcuate nucleus of the hypothalamus plays a crucial role in puberty onset, representing a significant advance in our understanding of reproductive biology. The work, which represents a fundamental advance, employs innovative approaches and benefits from the combined expertise of two respected laboratories, enhancing the robustness of the findings. Given the potential impact on human health and the strength of the evidence presented, this work will likely influence the field substantially and may inform future clinical applications.

   Reviewed by eLife

2. MLCK/MLCP regulates mammalian axon regeneration via the redistribution of the growth cone F-actin

   This article has 5 authors:

   1. Saijilafu
   2. Wei-Hua Wang
   3. Jin-Jin Ma
   4. Yin Yin
   5. Yan-Xia Ma

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Saijilafu et al. describe that MLCK and MLCP bidirectionally regulate NMII phosphorylation ultimately impinging on axonal growth during regeneration in the central and peripheral nervous systems. However, the evidence is in most cases incomplete, since some key controls are missing, some major claims are too broad to be supported by data and some claims and evidence present internal contradictions. In sum, this knowledge is potentially useful for the field due to the relevance of identifying mechanisms that regulate axonal regeneration, providing some claims inconsistencies are better supported and properly discussed.

   Reviewed by eLife

3. Coordinated regulation of chemotaxis and resistance to copper by CsoR in Pseudomonas putida

   This article has 9 authors:

   1. Meina He
   2. Yongxin Tao
   3. Kexin Mu
   4. Haoqi Feng
   5. Ying Fan
   6. Tong Liu
   7. Qiaoyun Huang
   8. Yujie Xiao
   9. Wenli Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Data presented in this useful report suggest a potentially new model for chemotaxis regulation in the gram-negative bacterium P. putida. Data supporting interactions between CheA and the copper-binding protein CsoR, reveal potential mechanisms for coordinating chemotaxis and copper resistance. There was, however concern about the large number of CheA interactors identified in the initial screen and it was felt that the study was incomplete without a substantial number of additional experiments to test the model and bolster the authors' conclusions.

   Reviewed by eLife

4. BCAS2 promotes primitive hematopoiesis by sequestering β-catenin within the nucleus

   This article has 9 authors:

   1. Guozhu Ning
   2. Yu Lin
   3. Haixia Ma
   4. Jiaqi Zhang
   5. Liping Yang
   6. Zhengyu Liu
   7. Lei Li
   8. Xinyu He
   9. Qiang Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this important study, the findings have theoretical and practical implications beyond a single subfield; the work supports the role of breast carcinoma amplified sequence 2 (Bcas2) in positively regulating primitive wave hematopoiesis through amplification of beta-catenin-dependent (canonical) Wnt signaling. The study is convincing, using appropriate and validated methodology in line with the current state-of-the-art; there is a first-rate analysis of a strong phenotype with highly supportive mechanistic data. The findings shed light on the controversial question of whether, when, and how canonical Wnt signaling may be involved in hematopoietic development. The work will be of interest to hematologists but also to developmental biologists.

   Reviewed by eLife

5. Integration of ATAC and RNA-sequencing identifies chromatin and transcriptomic signatures in classical and non-classical zebrafish osteoblasts and indicates mechanisms of entpd5a regulation

   This article has 4 authors:

   1. Kleio Petratou
   2. Martin Stehling
   3. Ferenc Müller
   4. Stefan Schulte-Merker

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work describes for the first time the combined gene expression and chromatin structure at the genome level in isolated chondrocytes and classical (cranial) and non-classical (notochordal) osteoblasts. In a compelling analysis of RNA-Seq and ATAC data, the authors characterize the two osteoblast populations relative to their associated chondrocyte cells and further proceed with a convincing analysis of the crucial entpd5a gene regulatory elements by investigating their respective transcriptional activity and specificity in developing zebrafish.

   Reviewed by eLife

6. WNKs regulate mouse behavior and alter central nervous system glucose uptake and insulin signaling

   This article has 7 authors:

   1. Ankita B Jaykumar
   2. Derk Binns
   3. Clinton A Taylor
   4. Anthony Anselmo
   5. Shari G Birnbaum
   6. Kimberly M Huber
   7. Melanie H Cobb

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study seeks to expand the understanding of insulin and glucose responses in the brain, specifically by implicating a family of protein kinases responsive to insulin. The significance of the study to the field is valuable. The evidence supporting the conclusions about brain glucose utilization is convincing, although there are several aspects that could benefit from additional validation to strengthen the claims.

   Reviewed by eLife

7. APP β-CTF triggers cell-autonomous synaptic toxicity independent of Aβ

   This article has 9 authors:

   1. Mengxun Luo
   2. Jia Zhou
   3. Cailu Sun
   4. Wanjia Chen
   5. Chaoying Fu
   6. Chenfang Si
   7. Yaoyang Zhang
   8. Yang Geng
   9. Yelin Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a useful demonstration that a specific protein fragment may induce the loss of synapses in Alzheimer's disease. The evidence supporting the data is solid but incomplete and would benefit from additional experiments. The application of the findings is limited because blocking the formation of the protein fragment has not benefited patients in several clinical trials.

   Reviewed by eLife

8. Translatome analysis reveals cellular network in DLK-dependent hippocampal glutamatergic neuron degeneration

   This article has 6 authors:

   1. Erin M Ritchie
   2. Siming Zhong
   3. Qianyi Pu
   4. Yunbo Li
   5. Binhai Zheng
   6. Yishi Jin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript describes the impact of modulating signaling by a key regulatory enzyme, Dual Leucine Zipper Kinase (DLK), on hippocampal neurons. The results are interesting and will be important for scientists interested in synapse formation, axon specification, and cell death. The methods and interpretation of the data are solid, but the study can be further strengthened with some additional studies and controls.

   Reviewed by eLife

9. α/β-Hydrolase domain-containing 6 (ABHD6) accelerates the desensitization and deactivation of TARP γ-2-containing AMPA receptors

   This article has 14 authors:

   1. Rixu Cong
   2. Huiran Li
   3. Hong Yang
   4. Jing Gu
   5. Shanshan Wang
   6. Xiangyu Guan
   7. Tangyunfei Su
   8. Yunlin Zheng
   9. Dianchun Wang
   10. Xinran Chen
   11. Lei Yang
   12. Yun Stone Shi
   13. Mengping Wei
   14. Chen Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental work demonstrates that ABHD6 regulates AMPAR gating kinetics in a TARP γ-2-dependent manner. The evidence in this study is compelling. This study will be of interest to readers in the field of synaptic transmission.

   Reviewed by eLife

10. Cryo-electron tomography reveals the microtubule-bound form of inactive LRRK2

    This article has 8 authors:

    1. Siyu Chen
    2. Tamar Basiashvili
    3. Joshua Hutchings
    4. Marta Sanz Murillo
    5. Amalia Villagran Suarez
    6. Jaime Alegrio Louro
    7. Andres E Leschziner
    8. Elizabeth Villa

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      In this manuscript, Chen et al. used cryo-ET and in vitro reconstituted system to demonstrate that the autoinhibited form of LRRK2 can also assemble into filaments on the microtubule surface, with a new interface involving the N-terminal repeats that were disordered in the previous active-LRRK2 filament structure. The structure obtained in this study is the highest resolution of LRRK2 filaments done by subtomogram averaging, representing a major technical advance compared to the previous paper from the same group. This is an important study, especially considering the pharmacological implications of the effect of inhibitors of the protein. The strengths of the data are convincing, but the study would be considerably strengthened if the authors addressed several discrepancies relating to their earlier work, and explored the physiological significance of the new interfaces and the incomplete decoration of microtubules described here.

    Reviewed by eLife