eLife

Latest preprint reviews

1. Antibiotic potentiation and inhibition of cross-resistance in pathogens associated with cystic fibrosis

   This article has 11 authors:

   1. Nikol Kadeřábková
   2. R Christopher D Furniss
   3. Evgenia Maslova
   4. Kathryn E Potter
   5. Lara Eisaiankhongi
   6. Patricia Bernal
   7. Alain Filloux
   8. Cristina Landeta
   9. Diego Gonzalez
   10. Ronan R McCarthy
   11. Despoina AI Mavridou

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study demonstrates that disruption of a common protein-folding system renders drug-resistant clinical bacteria susceptible to antibiotics. The work convincingly shows that targeting protein folding can be used to combat multidrug-resistant pathogens, both by potentiating the efficacy of existing drugs and by therapeutic use of small-molecule inhibitors. This study is significant and timely as it informs on a new strategy that is relevant to microbiologists and clinicians interested in combating antimicrobial resistance.

   Reviewed by eLife

2. Early acquisition of S-specific Tfh clonotypes after SARS-CoV-2 vaccination is associated with the longevity of anti-S antibodies

   This article has 7 authors:

   1. Xiuyuan Lu
   2. Hiroki Hayashi
   3. Eri Ishikawa
   4. Yukiko Takeuchi
   5. Julian Vincent Tabora Dychiao
   6. Hironori Nakagami
   7. Sho Yamasaki

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study by Lu et al aimed to determine the key factors of T cell responses associated with durable antibody responses following the initial two shots of COVID-19 mRNA vaccinations. By comparing the SARS-CoV-2 spike protein (S)-specific T cell subsets between "Ab sustainers" and "Ab decliners" that were present post-vaccination, the authors concluded that S-specific CD4+ T cells in "Ab sustainers" were enriched with Tfh cells. There is solid evidence as the authors applied multiple methods and approaches to address the key questions, and the presented data are robust.

   Reviewed by eLife

3. Direct observation of the neural computations underlying a single decision

   This article has 6 authors:

   1. Natalie Steinemann
   2. Gabriel M Stine
   3. Eric Trautmann
   4. Ariel Zylberberg
   5. Daniel M Wolpert
   6. Michael N Shadlen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental work quantifies the stochastic dynamics of neural population activity in the lateral intraparietal area (LIP) of the macaque monkey brain during single perceptual decisions. These single-trial dynamics have been subject to intense debate in neuroscience, and they have significant implications for modelling decision-making in various fields including neuroscience and psychology. Through a combination of state-of-the-art recordings from many LIP neurons and theory-driven data analyses, the authors provide convincing evidence for the notion that single-trial neural population dynamics in LIP encode the decision variable postulated by the drift-diffusion model of decision-making.

   Reviewed by eLife

4. SPAG7 deletion causes intrauterine growth restriction, resulting in adulthood obesity and metabolic dysfunction

   This article has 18 authors:

   1. Stephen E Flaherty
   2. Olivier Bezy
   3. Brianna LaCarubba Paulhus
   4. LouJin Song
   5. Mary Piper
   6. Jincheng Pang
   7. Yoson Park
   8. Shoh Asano
   9. Yu-Chin Lien
   10. John D Griffin
   11. Andrew Robertson
   12. Alan Opsahl
   13. Dinesh Hirenallur Shanthappa
   14. Youngwook Ahn
   15. Evanthia Pashos
   16. Rebecca A Simmons
   17. Morris J Birnbaum
   18. Zhidan Wu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study combines molecular genetics and target validation to discover genes involved in obesity and determine their role. It was unanimously agreed that the work is important in terms of significance as it has conceptual and practical implications beyond metabolism, including embryonic and placental development. The strength of evidence is convincing from the use of their forward genetic screen in mice.

   Reviewed by eLife

5. Imaging analysis of six human histone H1 variants reveals universal enrichment of H1.2, H1.3, and H1.5 at the nuclear periphery and nucleolar H1X presence

   This article has 3 authors:

   1. Monica Salinas-Pena
   2. Elena Rebollo
   3. Albert Jordan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript is an important advance in the study of Histone H1s, finding distinct distributions of various H1 variants in the genome. The controls presented by the authors provide convincing evidence to demonstrate a heterogenous distribution of H1 which might reflect functional regulation of chromatin accessibility by linker histones. This work will be of interest to the genome organization field, and could additionally provide a framework for understanding H1 mis-regulation observed in cancer cells.

   Reviewed by eLife

6. Combining array tomography with electron tomography provides insights into leakiness of the blood-brain barrier in mouse cortex

   This article has 12 authors:

   1. Georg Kislinger
   2. Gunar Fabig
   3. Antonia Wehn
   4. Lucia Rodriguez
   5. Hanyi Jiang
   6. Cornelia Niemann
   7. Andrey S Klymchenko
   8. Nikolaus Plesnila
   9. Thomas Misgeld
   10. Thomas Müller-Reichert
   11. Igor Khalin
   12. Martina Schifferer

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The present paper describes an important methodological development that combines light (confocal) microscopy with scanning and transmission EM and EM tomography. The method expands the level of structural detail accessible to large-volume EM studies and thus represents an approach to integrate analyses of cellular and sub-cellular structures in biological samples. The study, which provides a compelling proof-of-principle, will be of particular value to cell biologists interested in the in-depth interpretation of high-resolution ultrastructural information from sparsely distributed targets - at multiple scales and in diverse biological structures.

   Reviewed by eLife

7. Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius

   This article has 12 authors:

   1. Lavinia Gambelli
   2. Mathew McLaren
   3. Rebecca Conners
   4. Kelly Sanders
   5. Matthew C Gaines
   6. Lewis Clark
   7. Vicki AM Gold
   8. Daniel Kattnig
   9. Mateusz Sikora
   10. Cyril Hanus
   11. Michail N Isupov
   12. Bertram Daum

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental work substantially advances our structural understanding of S-layers in Archaea and how they are built to form formidable cell support structures able to stabilise the cytoplasmic membrane under harsh physicochemical conditions. The supporting evidence for the S-layer model is convincing, making excellent use of state-of-the-art 3D cryo-electron tomography reconstructions, although the proposed S-layer model would benefit from some additional validation.

   Reviewed by eLife

8. Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells

   This article has 4 authors:

   1. Lars N Royall
   2. Diana Machado
   3. Sebastian Jessberger
   4. Annina Denoth-Lippuner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript reports important findings in the field of developmental neurobiology, particularly in our understanding of human cortical development. The methods, data, and analyses are solid yet, the lack of clonal resolution or timelapse imaging makes it hard to assess whether the inheritance of centrosomes occurs as the authors claim.

   Reviewed by eLife

9. scMultiome analysis identifies embryonic hindbrain progenitors with mixed rhombomere identities

   This article has 3 authors:

   1. Yong-Il Kim
   2. Rebecca O'Rourke
   3. Charles G Sagerström

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study transcriptomically profiles the developing zebrafish hindbrain from gastrulation through stages of rhombomere formation. The strength is that the transcriptomic data will be a valuable resource to the field. The paper would profit from a deeper analysis of functional aspects of hindbrain development during its segmentation into rhombomeres.

   Reviewed by eLife

10. ACC neural ensemble dynamics are structured by strategy prevalence

    This article has 3 authors:

    1. Mikhail Proskurin
    2. Maxim Manakov
    3. Alla Karpova

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This manuscript posits a novel role for the anterior cingulate cortex (ACC) in coding for sequential action strategies and the prevalence of each strategy. These findings provide important insight into ACC function and will therefore be of broad interest within the field of cognitive neuroscience. The evidence supporting the primary hypothesis is currently incomplete but could be rendered convincing with some further effort to rule out potential confounding factors.

    Reviewed by eLife