eLife

Latest preprint reviews

1. Individual differences in fear memory expression engage distinct functional brain networks

   This article has 6 authors:

   1. Barbara D Fontana
   2. Jacob Hudock
   3. Neha Rajput
   4. Dea Kanini
   5. Dinh Luong
   6. Justin W Kenney

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work sets out to identify the neural substrates of associative fear responses in adult zebrafish. Through a compelling and innovative paradigm and analysis, the authors suggest brain regions associated with individual differences in fear memory. While several findings are well supported, aspects of the interpretation and presentation are partially incomplete, and the manuscript would benefit from adjusting key claims or including additional experiments. Nonetheless, this study showcases the strength of zebrafish for systems-level neuroscience and will be of broad interest to the neuroscience community.

   Reviewed by eLife

2. Viral commitment to infection depends on host metabolism

   This article has 4 authors:

   1. Anastasios Marantos
   2. Kim Sneppen
   3. Stanley Brown
   4. Namiko Mitarai

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study shows the impact of the metabolic state of bacteria on phage infection. The experimental results, based on various phages infecting E. coli, are solid and consistent with a two-step adsorption mathematical model, although the detailed evidence supporting this model is currently incomplete. This study should be of interest to the communities working on cell metabolism and on host-pathogen interactions.

   Reviewed by eLife

3. Cortical motor activity modulates respiration and reduces apnoea in neonates

   This article has 8 authors:

   1. Coen S Zandvoort
   2. Fatima Usman
   3. Shellie Robinson
   4. Odunayo Fatunla
   5. Eleri Adams
   6. Kyle TS Pattinson
   7. Simon F Farmer
   8. Caroline Hartley

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Zandvoort and colleagues describe respiration-brain coupling in the context of apnoea in human newborns. The authors have addressed an important question and supported their claims with solid data. The rigor of the findings could perhaps be further strengthened with some relatively minor changes to the analysis methodology.

   Reviewed by eLife

4. Beta-Glucan Modulates Monocyte Plasticity and Differentiation Capacity to Mitigate DSS-Induced Colitis

   This article has 16 authors:

   1. Yinyin Lv
   2. Yanyun Fan
   3. Qingxiang Gao
   4. Qiongyun Chen
   5. Yiqun Hu
   6. Lin Wang
   7. Huaxiu Shi
   8. Ermei Chen
   9. Qinyu Xu
   10. Ying Cai
   11. Qingqi Fan
   12. Linying Li
   13. Dan Du
   14. Jianlin Ren
   15. Shih-Chin Cheng
   16. Hongzhi Xu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents valuable and compelling evidence that β-glucan-induced trained immunity can protect against intestinal inflammation by reprogramming innate immune cells toward a reparative phenotype. The authors employ a convincing combination of functional assays, adoptive transfers, and single-cell transcriptomics to uncover mechanistic insights and demonstrate the therapeutic potential of innate immune memory in IBD. While the work is robust, addressing the underlying epigenetic mechanisms and including additional controls would further reinforce the trained immunity-specific interpretation.

   Reviewed by eLife

5. Synthetic auxotrophy reveals metabolic regulation of plasma cell generation, affinity maturation, and cytokine receptor signaling

   This article has 16 authors:

   1. Sung Hoon Cho
   2. Shawna K Brookens
   3. Ariel L Raybuck
   4. Kaylor Meyer
   5. Yeeun C Paik
   6. Jennie Hamilton
   7. Jingxin Li
   8. Karel Kalecky
   9. Chloe Park
   10. Sakeenah L Hicks
   11. John Karijolich
   12. Teodoro H Bottiglieri
   13. Jeffrey C Rathmell
   14. Denis Mogilenko
   15. Chris D Scharer
   16. Mark R Boothby

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors addressed an important biological question, namely the role of glutamine metabolism in humoral responses, and they obtained solid conclusions. The strength of this study is that the authors used state-of-the-art transgenic mouse models together with in vitro analysis, thereby providing significant insights into the question posed. The following would strengthen the manuscript: i) adding more in-depth functionality/physiological relevance in the discussion part, and ii) regarding the experiments, the inclusion of more appropriate controls and a clearer and more accurate description of the methods.

   Reviewed by eLife

6. PHD1-dependent hydroxylation of RepoMan (CDCA2) on P604 modulates the control of mitotic progression

   This article has 12 authors:

   1. Jimena Druker
   2. Hao Jiang
   3. Dilem Shakir
   4. Fraser Child
   5. Vanesa Alvarez
   6. Melpomeni Platani
   7. Andrea Corno
   8. Constance Alabert
   9. Adrian T Saurin
   10. Jason R Swedlow
   11. Sonia Rocha
   12. Angus I Lamond

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This compelling work describes how the cell cycle-regulating phosphatase subunit, RepoMan, is regulated by the oxygen-dependent, metabolite-sensing hydroxylase PHD1. The characterisation of how proline hydroxylation alters signalling at the molecular and cellular level provides important evidence to enhance our understanding of how 2-oxoglutarate-dependent dioxygenases influence the cell cycle and mitosis.

   Reviewed by eLife

7. SLC4A1 mutations that cause distal renal tubular acidosis alter cytoplasmic pH and cellular autophagy

   This article has 12 authors:

   1. Grace Essuman
   2. Midhat Rizvi
   3. Ensaf Almomani
   4. Shahid AK M Ullah
   5. Sarder MA Hasib
   6. Forough Chelangarimiyandoab
   7. Priyanka Mungara
   8. Manfred J Schmitt
   9. Marguerite Hureaux
   10. Rosa Vargas-Poussou
   11. Nicolas Touret
   12. Emmanuelle Cordat

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work characterizes the function and localization of SLC4A1 variants associated with distal renal tubular acidosis in human patients. Cell culture and limited animal studies provide partial but incomplete support to the authors' claim that the variants disrupt normal protein degradative flux by alkalinizing the intracellular pH. The study is valuable in providing preliminary evidence for future exploration of the link between intracellular pH regulation by SLC4A1 and kidney cell function in vivo.

   Reviewed by eLife

8. Optimised genome editing for precise DNA insertion and substitution using Prime Editors in zebrafish

   This article has 11 authors:

   1. Yosuke Ono
   2. Martin Peterka
   3. Michael Love
   4. Ashish Bhandari
   5. Euan Gordon
   6. Jonathan S Ball
   7. Charles R Tyler
   8. Steve Rees
   9. Mohammad Bohlooly-Y
   10. Marcello Maresca
   11. Steffen Scholpp

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable comparison of the efficiency and precision of two prime editing methods to introduce single-nucleotide variants and longer exogenous DNA sequences into the zebrafish genome. Solid data support the conclusion that the PE2 prime editor Nickase is more effective at introducing single-nucleotide variants, while the PEn prime editor nuclease is more effective at integrating short sequences from 3 up to 30 base pairs, for both somatic and germline editing. The results will be of interest to the zebrafish community, in particular to model human disease variants in this model organism.

   Reviewed by eLife

9. Decoding movie content from neuronal population activity in the human medial temporal lobe

   This article has 12 authors:

   1. Franziska Gerken
   2. Alana Darcher
   3. Pedro J Gonçalves
   4. Rachel Rapp
   5. Ismail Elezi
   6. Johannes Niediek
   7. Marcel S Kehl
   8. Thomas P Reber
   9. Stefanie Liebe
   10. Jakob H Macke
   11. Florian Mormann
   12. Laura Leal-Taixé

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study demonstrates that it is possible to decode information about characters and locations from single-unit responses in the human brain to a narrative movie, using a convincing technical approach to capture information in population-level dynamics. The study introduces an exciting dataset of single-unit responses in humans during a naturalistic and dynamic movie stimulus, with recordings from multiple regions within the medial temporal lobe. Using both a traditional firing-rate analysis as well as a population decoding analysis to connect these neural responses to the visual content of the movie, they show that in this dataset, the decoding of semantic scene features (e.g., the person currently on screen), but not scene transitions, is surprisingly driven by classically non-responsive neurons. Based on these findings, the authors argue that dynamic naturalistic semantic information may be processed within the medial temporal lobe at the population level.

   Reviewed by eLife

10. Specific GPCRs Elicit Unique Extracellular Vesicle MiRNA Array Signatures: An Exploratory Study

    This article has 6 authors:

    1. Xiao Shi
    2. Michelle C Palumbo
    3. Sheila Benware
    4. Jack Wiedrick
    5. Sheila Markwardt
    6. Aaron Janowsky

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This study presents valuable findings by demonstrating that specific GPCR subtypes induce distinct extracellular vesicle miRNA signatures, highlighting a potential novel mechanism for intercellular communication with implications for receptor pharmacology within the field. The data is compelling, however, more evidence is needed to determine whether the distinct extracellular vesicle miRNA signatures result from GPCR-dependent miRNA expression or GPCR-dependent incorporation of miRNAs into extracellular vesicles.

    Reviewed by eLife