eLife

Latest preprint reviews

1. Associations Between Meteorological Factors and Influenza A/B Incidence in Subtropical China: A Six-Year Surveillance Study with Deep Learning Modelling for Influenza Early Warning

   This article has 14 authors:

   1. Long Xie
   2. Meng-Jie Zhang
   3. Jin-Lin Tan
   4. Yi-Xin Ling
   5. Zhe-Qiang Xue
   6. Jun-Ju Huang
   7. Jian-Ling Chen
   8. Ze-Fan Ruan
   9. Jing Qian
   10. Hai-Yong Pan
   11. Xiao Han
   12. Sheng Xiong
   13. Long-Mei Ling
   14. Xi-Wen Jiang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study is a valuable contribution to the evidence base. However, the evidence provided is incomplete as the study results only partially support the study conclusions. Addressing the methodological and reporting issues raised by the peer reviewers and properly aligning the claim made for providing a tool for early warning with the study analysis/results would improve the study quality and usefulness of its findings.

   Reviewed by eLife

2. A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments

   This article has 5 authors:

   1. Annemiek C Veldsink
   2. Jonas S Fischer
   3. Sophie Hell
   4. Karsten Weis
   5. Liesbeth M Veenhoff

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study introduces a non-perturbative pulse-labeling strategy for yeast nuclear pore complexes (NPCs), employing a nanobody-based approach in order to selectively capture Nup84-containing complexes for imaging and biochemical analysis. The data convincingly demonstrate that a short induction period (20 minutes to 1 hour) yields a strong and sustained signal, enabling affinity purification that faithfully recapitulates the endogenous Nup84 interactome. This tool offers a powerful framework for investigating NPC dynamics and associated interactomes through both imaging and biochemical assays.

   Reviewed by eLife

3. Type I and type III interferon receptor knockout chickens: Novel models for unraveling interferon dynamics in influenza infection

   This article has 18 authors:

   1. Mohanned Naif Alhussien
   2. Hanna-Kaisa Vikkula
   3. Romina Klinger
   4. Christian Zenner
   5. Simon P Früh
   6. Rashi Negi
   7. Theresa von Heyl
   8. Sabrina Schleibinger
   9. Milena Brunner
   10. Tom VL Berghof
   11. Leora Avolio
   12. Arne Reich
   13. Benjamin Schade
   14. Bassel A Abukhadra
   15. Silke Rautenschlein
   16. Rudolf Preisinger
   17. Hicham Sid
   18. Benjamin Schusser

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study reports on the development and characterization of chickens with genetic deficiencies in type I or type III interferon receptors, which is an important contribution to the field of avian immunology. The data reflecting the development of the new interferon-receptor-deficient chickens is compelling. However, the characterization of IFN biology and infection responses in these knockout chickens is somewhat incomplete and could be improved by addressing the noted weaknesses.

   Reviewed by eLife

4. Host and antibiotic jointly select for greater virulence in Staphylococcus aureus

   This article has 7 authors:

   1. Michelle Su
   2. Kim L Hoang
   3. McKenna Penley
   4. Michelle H Davis
   5. Jennifer D Gresham
   6. Levi T Morran
   7. Timothy D Read

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study examines the evolution of virulence and antibiotic resistance in Staphylococcus aureus under multiple selection pressures. The evidence presented is convincing, with rigorous data that characterizes the outcomes of the evolution experiments. However, the manuscript's primary weakness is in its presentation, as claims about the causal relationship between genotypes and phenotypes are based on correlational evidence. The manuscript needs to be revised to address these limitations, clarify the implications of the experimental design, and adjust the overall narrative to better reflect the nature of the findings.

   Reviewed by eLife

5. Depletion of extracellular asparagine impairs self-reactive T cells and ameliorates autoimmunity in a murine model of multiple sclerosis

   This article has 15 authors:

   1. Peter Georgiev
   2. Sheila Johnson
   3. Kiran Kurmi
   4. Song-Hua Hu
   5. SeongJun Han
   6. Dillon Patterson
   7. Thao H Nguyen
   8. Linglin Huang
   9. Dan Liang
   10. Naomi Goldman
   11. Thomas Conway
   12. Hannah Creasey
   13. Jared Rowe
   14. Marcia C Haigis
   15. Arlene H Sharpe

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Non-essential amino acids such as glutamine have been known to be required for T cell general activation through sustaining basic biosynthetic processes, including nucleotide biosynthesis, ATP generation, and protein synthesis. In this important study, the authors found that extracellular asparagine (Asn) is required not only for T cells to generally refuel metabolic reprogramming, but to produce helper T cell lineage-specific cytokine, for instance, IL17. In particular, the importance of Asn in IL17 production was convincingly demonstrated in the mouse experimental autoimmune encephalomyelitei (EAE) model, mimicking human multiple sclerosis disease.

   Reviewed by eLife

6. Tolerance to Lung Infection in TWIK2 K+ Efflux Mediated Macrophage Trained Immunity

   This article has 6 authors:

   1. Josh Thompson
   2. Yufan Li
   3. Yuanling Song
   4. Ki-Wook Kim
   5. Asrar B Malik
   6. Jingsong Xu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents valuable data suggesting that ATP-induced modulation of alveolar macrophage (AM) functions is associated with NLRP3 inflammasome activation and enhanced phagocytic capacity. While the in vivo and in vitro data reveal an interesting phenotype, the evidence provided is incomplete and does not fully support the paper's conclusions. Additional investigations would be of value in complementing the data and strengthening the interpretation of the results. This study should be of interest to immunologists and the mucosal immunity community.

   Reviewed by eLife

7. Quantitative RNA pseudouridine landscape reveals dynamic modification patterns and evolutionary conservation across bacterial species

   This article has 10 authors:

   1. Letong Xu
   2. Shenghai Shen
   3. Yizhou Zhang
   4. Zhihao Guo
   5. Beifang Lu
   6. Jiadai Huang
   7. Runsheng Li
   8. Yitong Shen
   9. Li-Sheng Zhang
   10. Xin Deng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study illustrates a valuable application of BID-seq to bacterial RNA, allowing transcriptome-wide mapping of pseudouridine modifications across various bacterial species. The evidence presented includes a mix of solid and incomplete data and analyses, and would benefit from more rigorous approaches. The work will interest a specialized audience involved in RNA biology.

   Reviewed by eLife

8. Starvation of the bacteria Vibrio atlanticus promotes lightning group-attacks on the dinoflagellate Alexandrium pacificum

   This article has 13 authors:

   1. Jean Luc Rolland
   2. Estelle Masseret
   3. Mohamed Laabir
   4. Guillaume Tetreau
   5. Benjamin Gourbal
   6. Anne Thebault
   7. Eric Abadie
   8. Alice Rodrigues-Stien
   9. Carole Veckerlé
   10. Elodie Servanne-Meunier
   11. Delphine Destoumieux-Garzón
   12. Arnaud Lagorce
   13. Raphaël Lami

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study convincingly shows that Vibrio bacteria act as predators of ecologically significant algae that contribute to harmful blooms in the lab, as well as in their natural habitat. While the data strongly suggest that starvation may induce predation, further work is needed to fully establish this link. Similarly, the evidence for a social component in the predation process remains incomplete. This study will be very impactful to those interested in the diversity of microbial predator-prey interactions and controlling toxic algal bloom, but the paper could be strengthened by more clearly showing the degree of replication, by better defining the terms used to describe the observed behaviour, and by providing better support for starvation and collective behaviour.

   Reviewed by eLife

9. Realistic coupling enables flexible macroscopic traveling waves in the mouse cortex

   This article has 4 authors:

   1. Guanhua Sun
   2. James Hazelden
   3. Ruby Kim
   4. Daniel B Forger

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable work presents a novel computational framework for modeling macroscopic traveling waves in the mouse cortex by integrating open-source connectomic and transcriptomic data into a spiking network model. This approach allows the computational model to assign excitatory/inhibitory connections based on neurotransmitter profiles and extends simulations to the 3D domain. The authors present results that demonstrate how spatiotemporal dynamics such as slow oscillations (0.5-4 Hz) emerge and self-organize at the whole-brain scale. This study provides convincing initial insights into the structural basis of traveling waves at the whole-brain scale in the mouse.

   Reviewed by eLife

10. Neural Connectome of the Ctenophore Statocyst

    This article has 5 authors:

    1. Kei Jokura
    2. Sanja Jasek
    3. Lara Niederhaus
    4. Pawel Burkhardt
    5. Gáspár Jékely

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This fundamental work significantly advances our understanding of gravity sensing and orientation behavior in the ctenophore, an animal of major importance in understanding the evolution of nervous systems. Through comprehensive reconstruction with volumetric electron microscopy, and time-lapse imaging of cilia motion, the authors provide compelling evidence that the aboral nerve net coordinates the activity of balancer cilia. The resemblance to the ciliomotor circuit in marine annelids provides a fascinating example of how neural circuits may convergently evolve to solve common sensorimotor challenges.

    Reviewed by eLife