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  1. Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cord in vivo

    This article has 4 authors:
    1. Yu-Ting Cheng
    2. Kawasi M. Lett
    3. Chris Xu
    4. Chris B. Schaffer
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this work, the authors put forward a valuable methodological advancement for imaging deeper in the intact spinal cord of anaesthetized mice. The authors measured blood flow across different vessel types within the spinal cord and observed the cellular responses following venule occlusion. The demonstration is solid, although, a more quantitative comparison with state-of-the-art two-photon excited fluorescence microscopy and a discussion about applicability to functional imaging (e.g., calcium imaging) would have strengthened the study.

    Reviewed by eLife

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