eLife

Latest preprint reviews

1. Multi-area activity in mouse motor cortex associated with one- and two-handed oromanual dexterity

   This article has 4 authors:

   1. John M Barrett
   2. Joshua I Glaser
   3. Andrew Miri
   4. Gordon MG Shepherd

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study documents, for the first time, the degree of preference for contralateral versus ipsilateral forelimb movements in the motor cortex of mice, revealing subtly distinct profiles across cortical areas, with the orofacial region showing comparatively little limb selectivity relative to the forelimb motor areas. The experiments and analyses are, in general, solid and well-designed, and the data support the paper's conclusions.

   Reviewed by eLife

2. Identifying molecular instructions to hard-wire a sensory neuron's synaptic connectivity

   This article has 13 authors:

   1. Júnia Vieira dos Santos
   2. Renee Yin Yu
   3. Andrea Terceros
   4. Peter Mussells Pires
   5. Denisa Rusu
   6. Vedrana Cvektkovska
   7. Alyeri Bucio-Méndez
   8. Tsung-Jung Lin
   9. Farida Emran
   10. Haig Djambazian
   11. Pierre Bérubé
   12. Robert Sladek
   13. Brian E Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study addresses how the molecular identity of a single neuron specifies its hard-wired synaptic connectivity, using repeated single-cell RNA sequencing of identified Drosophila sensory neurons together with functional perturbation of candidate cell-surface molecules. It demonstrates remarkably low transcriptomic variability across animals for the same identified neuron, defines a tractable set of differentially expressed cell-surface molecules that distinguish mechanosensory from chemosensory neurons, and links several of these molecules to axonal targeting and circuit function. The evidence is solid, with the single-neuron transcriptomic datasets and Dscam isoform repertoires offering a lasting resource for the field, though clearer articulation of the experimental logic, additional controls in the RNAi screen, and a more complete characterization of the neuronal re-wiring would further strengthen the central claims.

   Reviewed by eLife

3. ITK deficiency protects against autoimmune pulmonary hemorrhage by promoting Amphiregulin-producing regulatory T cells

   This article has 8 authors:

   1. Mohammad Saddam Hossain
   2. Ali Mobeen
   3. Ibrahim H Topkaya
   4. Hui Xiong
   5. Rosanne Trevail
   6. Li Chen
   7. Liye Suo
   8. Mobin Karimi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study investigates the role of Interleukin-2-inducible T cell kinase (ITK) deficiency in autoimmune lung injury using a pristane-induced pulmonary hemorrhage (PH) model, suggesting that ITK-deficient regulatory T cells (Tregs) restrict severe tissue pathology. The work represents an important addition to the fields of autoimmunity, inflammation, and T-cell biology in the lung. The experimental evidence supporting the core ideas is solid and provides mechanistic insight into how ITK deficiency promotes a tissue-protective Treg phenotype, although additional studies will be needed to further define the molecular pathways involved.

   Reviewed by eLife

4. Junctional β-Catenin Stabilization Links Wnt Signaling and Force Generation

   This article has 5 authors:

   1. Agimaa Otgonbaatar
   2. Sujithra Shankar
   3. Prameet Kaur
   4. Prabhat Tawari
   5. Nicholas S Tolwinski

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study by Otgonbaatar and colleagues employs advanced live microscopy, optogenetics, and an endogenous fluorescent timer system to investigate short- and long-term stabilization dynamics of β-catenin/Armadillo (Arm) during Drosophila development. The authors identify an unexpected and functionally relevant enrichment of stabilized junctional Arm in leading-edge cells during dorsal closure, providing evidence for a stabilization mechanism that appears independent of canonical Wingless signaling. These findings are significant because they expand current understanding of β-catenin/Arm beyond its canonical signaling functions and suggest a role in tissue mechanics and force transmission during dorsal closure. The proposed model represents a key advance in the field, but the strength of evidence is currently incomplete: the main conclusions regarding Wingless independence, JNK-mediated regulation, and the mechanical role of stabilized Arm are only partially supported by the available data and would benefit from further experimental testing and corroboration.

   Reviewed by eLife

5. Excitation–inhibition balance controls coupling stability and network reorganization in a plastic Kuramoto model

   This article has 2 authors:

   1. Satoshi Kuroki
   2. Kenji Mizuseki

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study investigates how plasticity and homeostatic adaptation can lead to the emergence of synchronization patterns associated with different sleep phases. The ideas are novel and interesting, but the evidence at present remains incomplete. Further work is needed to determine whether the reported states persist for larger system sizes, longer integration times, and how robust the results are to different initial conditions.

   Reviewed by eLife

6. Multiple molecular pathways to longevity with opposing gene expression programs defining distinct aging strategies in Caenorhabditis elegans

   This article has 9 authors:

   1. Zenith D Rudich
   2. Jiaxi Guan
   3. Aura A Tamez Gonzalez
   4. Grant F Booth
   5. Sonja K Soo
   6. Ulrich Anglas
   7. Meeta Mistry
   8. Megan M Senchuk
   9. Jeremy M Van Raamsdonk

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study presents a comparative analysis of the transcriptomic features underlying C. elegans longevity, providing insights into how different changes in gene expression can promote longevity. The authors present solid evidence with analysis and selected functional validation showing that some long-lived animals share common changes while others appear to use opposing strategies. The datasets and analyses contained within and the user-friendly website developed will be of interest to researchers interested in complicated transcriptomic analyses and/or the biology of aging.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

7. Asymmetric introgression and thermal advantage jointly drive climate-mediated lineage turnover in a mixed-ploidy reed

   This article has 8 authors:

   1. Lele Liu
   2. Wenyi Sheng
   3. Yuhui Wang
   4. Lele Lin
   5. Cui Wang
   6. Huijia Song
   7. Yaolin Guo
   8. Weihua Guo

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The report by Liu and colleagues reports on an analysis of environmental adaptation across diverse lineages of the grass Phragmites australis differing by their level of ploidy. These results represent important findings for understanding the environmental adaptation of species complexes with mixed ploidy and the analysis reports solid evidence that lineages with distinct levels of ploidy occupy different climate niches. The use of regional survey in tandem with common garden experiment represents a convincing approach to suggest a correlation between ploidy and climate adaptation. This manuscript will be of interest to a broad community of ecological genomicists interested in how structural variation in gene dosage potentially affects the pattern of adaptation.

   Reviewed by eLife

8. Identification of key Y4R residues enables the discovery of selective non-peptide small-molecule agonists

   This article has 12 authors:

   1. Tim Pelczyk
   2. Corinna Schüß
   3. Mateusz Skłodowski
   4. Fabian Liessmann
   5. David Jordan
   6. Vivian Ehrlich
   7. Paul Eisenhuth
   8. Jan Stichel
   9. Albert O Gattor
   10. Max Keller
   11. Jens Meiler
   12. Annette G Beck-Sickinger

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study combines peptide engineering, molecular docking, and functional assays to define the molecular basis of ligand recognition and activation of the human Y4 receptor and to identify three novel small-molecule agonists. The evidence supporting the conclusions is convincing, with complementary experimental and computational approaches providing strong support for the proposed receptor-ligand interactions. While concentration-response analyses of the small-molecule agonists and additional structural or mutagenesis studies would further strengthen the work, these are not essential to support the main conclusions. The work will be of interest to researchers studying GPCR pharmacology, structural biology, and ligand discovery.

   Reviewed by eLife

9. Open source laser-scanning photostimulation for neuroscience with Zapit

   This article has 20 authors:

   1. Michael Lohse
   2. Oliver M Gauld
   3. Maja T Quigley
   4. Ainiah Masood
   5. Chaofei Bao
   6. Jingjie Li
   7. Nathaniel J Miska
   8. Gerion Nabbefeld
   9. Quentin Pajot-Moric
   10. Simon Townsend
   11. Peter Vincent
   12. Angela Y Yang
   13. Nikolaos Zervogiannis
   14. Athena Akrami
   15. Chunyu A Duan
   16. Jeffrey C Erlich
   17. Sonja B Hofer
   18. Thomas D Mrsic-Flogel
   19. Philip Coen
   20. Robert AA Campbell

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work presents a software and hardware suite for targeted photostimulation that can be used in vivo. The package is a well-designed and documented hardware/software suite with a comprehensive build guide. This tool will likely promote important neuroscience advances through targeted real-time perturbation of the cerebral cortex. Overall, this manuscript makes a compelling case on how to design and make available power tools for the research community.

   Reviewed by eLife

10. Bacterial and viral gut microbiome alterations characterize microbiome-immune-pathophysiology axes in sickle cell disease

    This article has 11 authors:

    1. Zachary N Flamholz
    2. Jennifer De Los Santos
    3. Karen Ireland
    4. Janine Keenan
    5. Jacob S Kazmi
    6. Aakash Mahant Mahant
    7. Anayeli Correa
    8. Paul S Frenette
    9. Betsy C Herold
    10. Deepa Manwani
    11. Libusha Kelly

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This study presents a valuable metagenomic analysis of the gut microbiome in sickle cell disease (SCD) patients, revealing associations between bacteriophage, host immunity, and SCD pathophysiology. This solid dataset and in-depth analysis will be of broad interest to researchers studying SCD, immunology, phage biology, and the microbiome, adding to the small but growing literature suggesting a microbial component to SCD.

    Reviewed by eLife