eLife

Latest preprint reviews

1. Occupancy of the HbYX hydrophobic pocket is sufficient to induce gate opening in the archaeal 20S proteasomes

   This article has 3 authors:

   1. Janelle JY Chuah
   2. Madalena R Daugherty
   3. David M Smith

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable manuscript describes cryo-EM structures of archaeal proteasomes that reveal insights into how occupancy of binding pockets on the 20S proteasome regulates proteasome gating. The evidence supporting these claims is convincing, although the extrapolation of these findings to the more complex eukaryotic proteasome may prove challenging. This work will be of high interest to researchers interested in proteasome structure and regulation.

   Reviewed by eLife

2. The integration mechanism of the four positional cues—anterior, posterior, dorsal, and ventral—in axolotl limb regeneration

   This article has 5 authors:

   1. Sakiya Yamamoto
   2. Saya Furukawa
   3. Ayaka Ohashi
   4. Mayuko Hamada
   5. Akira Satoh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental work by Yamamoto and colleagues advances our understanding of how positional information is coordinated between axes during limb outgrowth and patterning. They provide solid evidence that the dorsal-ventral axis feeds into anterior-posterior signaling, and identify the responsible molecules by combining transplantations with molecular manipulations. This work will be of broad interest to regeneration, tissue engineering, and evolutionary biologists.

   Reviewed by eLife

3. Linage priming and cell type proportioning depends on the interplay between stochastic and deterministic factors

   This article has 7 authors:

   1. William M Salvidge
   2. Chris Brimson
   3. Nicole Gruenheit
   4. Li-Yao Huang
   5. Catherine J Pears
   6. Jason B Wolf
   7. Christopher RL Thompson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study investigates how stochastic and deterministic factors are integrated during cellular decision-making, particularly in situations where cells differentiate into distinct fates despite being exposed to the same environmental conditions. The authors present convincing evidence that gene expression variability contributes to the robustness of cell fate decisions in D. discoideum, which sheds light into the role of stochasticity during cell differentiation.

   Reviewed by eLife

4. Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells

   This article has 13 authors:

   1. Qi Zhang
   2. Yunfeng Wang
   3. Xueli Fu
   4. Ziguang Wang
   5. Yang Zhang
   6. Lizhong Yan
   7. Yuejia Wang
   8. Muhan Yang
   9. Dongze Song
   10. Ruixing Zhang
   11. Hongru Zhang
   12. Shian Wu
   13. Shaowei Zhao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study examines the role of E2 ubiquitin enzyme, Uev1a in tissue resistance to oncogenic RasV12 in Drosophila melanogaster polyploid germline cells and human cancer cell lines. The incomplete evidence suggests that Uev1a works with the E3 ligase APC/C to degrade Cyclin A, and the strength of evidence could be increased by addressing the expression of CycA in the ovaries and the uev1a loss of function in human cancer cells. This work would be of interest to researchers in germline biology and cancer.

   Reviewed by eLife

5. Structure of an oxygen-induced tubular nanocompartment in Pyrococcus furiosus

   This article has 11 authors:

   1. Wenfei Song
   2. Jan Fiala
   3. Ioannis Skalidis
   4. Pascal Albanese
   5. Constantinos Patinios
   6. Marten L Chaillet
   7. Servé WM Kengen
   8. Richard A Scheltema
   9. Stuart C Howes
   10. Albert JR Heck
   11. Friedrich Förster

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Using advanced CryoEM and mass spectrometry, the authors provide compelling evidence of how tubule formation occurs in an oxygen-dependent manner. These fundamental findings offer a novel mechanism by which rubrerythrin tubules encapsulate encapsulin to prevent oxidative stress in Pyrococcus furiosus. However, there are a few reasonable concerns about biochemical validations and the lack of adequate description of results and methodology.

   Reviewed by eLife

6. The denitrosylase SCoR2 controls cardioprotective metabolic reprogramming

   This article has 12 authors:

   1. Zachary W Grimmett
   2. Rongli Zhang
   3. Hua-Lin Zhou
   4. Qiuying Chen
   5. Dawson Miller
   6. Zhaoxia Qian
   7. Justin Lin
   8. Riti Kalra
   9. Steven S Gross
   10. Walter J Koch
   11. Richard T Premont
   12. Jonathan S Stamler

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents compelling evidence that the denitrosylase SCoR2 regulates cardioprotective metabolic reprogramming in the heart following ischemia/reperfusion injury. The findings are supported by a novel multi-omics approach and the integration of mouse and human data, which provides valuable insights into S-nitrosylation and cardiac metabolism. However, some conclusions remain limited by unresolved methodological issues that warrant clarification.

   Reviewed by eLife

7. SVEP1 enables efficient binding of Angiopoietin-2 to the TIE1 receptor, allowing receptor phosphorylation and downstream signaling

   This article has 9 authors:

   1. Katharina Uphoff
   2. Melina Hußmann
   3. Dörte Schulte-Ostermann
   4. Yvonne Huisman
   5. Matthias Mörgelin
   6. Fabian Metzen
   7. J Fernando Bazan
   8. Manuel Koch
   9. Stefan Schulte-Merker

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript focuses on developing a structural model of how the multidomain ECM protein SVEP1 enables Angiopoietin (ANG) binding to the orphan receptor TIE1, resulting in downstream receptor phosphorylation and signaling. This is a potentially important study, however, it currently lacks key controls and is therefore incomplete. The data will be of interest to scientists working in vascular biology and RTK signaling.

   Reviewed by eLife

8. High-throughput neutralization measurements correlate strongly with evolutionary success of human influenza strains

   This article has 13 authors:

   1. Caroline Kikawa
   2. Andrea N Loes
   3. John Huddleston
   4. Marlin D Figgins
   5. Philippa Steinberg
   6. Tachianna Griffiths
   7. Elizabeth M Drapeau
   8. Heidi Peck
   9. Ian G Barr
   10. Janet A Englund
   11. Scott E Hensley
   12. Trevor Bedford
   13. Jesse D Bloom

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study advances our understanding of population-level immune responses to influenza in both children and adults. The strength of the evidence supporting the conclusions is compelling, with high-throughput profiling assays and mathematical modeling. The work will be of interest to immunologists, virologists, vaccine developers, and those working on mathematical modeling of infectious diseases.

   Reviewed by eLife

9. Effects of Arginine Vasopressin on Islet Cells in Pancreatic Tissue Slices: Glucose-Dependent Modulation of IP3 Receptor-Specific Responses

   This article has 14 authors:

   1. Jasmina Kerčmar
   2. Nastja Murko
   3. Lidija Križančić Bombek
   4. Eva Paradiž Leitgeb
   5. Johannes Pfabe
   6. Sandra Postić
   7. Ya-Chi Huang
   8. Andraž Stožer
   9. Dean Korošak
   10. Xaver Kozisek
   11. Monika Perisic
   12. Markus Muttenthaler
   13. Christian W Gruber
   14. Marjan Slak Rupnik

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a useful finding on the effects of arginine vasopressin (AVP) on islet cells in pancreatic tissue slices, using technically sophisticated spatio-temporal calcium recordings to confirm that AVP influences α and β cells differently depending on glucose concentrations. While the study’s methods – particularly the calcium imaging techniques and peptide ligand design targeting V1b receptors – are strong, the reviewers were concerned about several aspects of the experimental design. The results on β-cell responses are incomplete and insufficient to support the manuscript’s claims, especially due to the high variability of islet responses and lack of mechanistic and functional (hormone release) data. There are also concerns about the possibility of off-target effects and suboptimal receptor specificity: the study would be significantly strengthened by inclusion of signaling pathway interrogation, hormone output assays, genetic validation (e.g., β cell-specific deletion of V1br), and receptor localization. The work will still be of interest to researchers studying islet physiology in the context of health and diabetes.

   Reviewed by eLife

10. Identifying a novel mechanism of L-leucine uptake in Mycobacterium tuberculosis using a chemical genomic approach

    This article has 8 authors:

    1. Nisheeth Agarwal
    2. Himanshu Gogoi
    3. Eeba
    4. Linus Augustin
    5. Md. Younus Khan
    6. Yashwant Kumar
    7. Sayan Kumar Bhowmick
    8. Bappaditya Dey

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      By performing a chemical screen of an FDA-approved library of small molecules against a leucine-dependent Mtb strain, this work discovered that semapimod inhibits Mtb growth by impairing leucine import. The work is useful because it connects leucine uptake with the cell wall lipids in Mtb; however, it remains incomplete as the evidence supporting semapimod's ability to target leucine uptake needs more substantial proof. The work requires significant experimental evidence to identify leucine transporter(s) and determine how PDIM participates in leucine uptake.

    Reviewed by eLife