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   10.1101/2023.01.13.523917

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   10.1101/2024.11.18.624089

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   10.1101/2024.11.13.623378

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   10.1101/2024.10.31.621279

5. SATAY-based chemogenomic screening uncovers antifungal resistance mechanisms and key determinants of ATI-2307 and chitosan sensitivity

   This article has 7 authors:

   1. Matthew T Karadzas
   2. Agnès H Michel
   3. Andreas Mosbach
   4. George Giannakopoulos
   5. Ruairi McGettigan
   6. Gabriel Scalliet
   7. Benoît Kornmann

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important manuscript proposes a new strategy for the identification of new mechanisms of drug resistance based on SAturated Transposon Analysis in Yeast (SATAY), a powerful transposon sequencing method in Saccharomyces cerevisiae. This method allows us to uncover loss- and gain-of-function mutations conferring resistance to 20 different antifungal compounds. The method is convincing, allowing the authors to identify a novel interaction of chitosan with the cell wall mannosylphosphate, and show that the transporter Hol1 concentrates the novel antifungal ATI-2307 within yeast.

   Reviewed by eLife

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   10.1101/2024.12.17.629038

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   10.1101/2024.11.25.625128

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   10.1101/2024.07.03.601861

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   10.1101/2024.10.14.618165

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    10.1101/2024.12.30.630740