eLife

Latest preprint reviews

1. Expanding automated multiconformer ligand modeling to macrocycles and fragments

   This article has 9 authors:

   1. Jessica Flowers
   2. Nathaniel Echols
   3. Galen J Correy
   4. Priyadarshini Jaishankar
   5. Takaya Togo
   6. Adam R Renslo
   7. Henry van den Bedem
   8. James S Fraser
   9. Stephanie A Wankowicz

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The work presents a valuable extension of qFit-ligand, a computational method for modeling conformational heterogeneity of ligands in X-ray crystallography and cryo-EM density maps. The authors provide solid evidence of improved capabilities through careful validation against the previous version, particularly in expanding ligand sampling within conformational space. Such improvements suggest practical utility for challenging applications, including macrocyclic compound modeling and crystallographic drug fragment screening.

   Reviewed by Arcadia Science, eLife

2. Molecular feature-based classification of retroperitoneal liposarcoma: a prospective cohort study

   This article has 10 authors:

   1. Mengmeng Xiao
   2. Xiangji Li
   3. Fanqin Bu
   4. Shixiang Ma
   5. Xiaohan Yang
   6. Jun Chen
   7. Yu Zhao
   8. Ferdinando Cananzi
   9. Chenghua Luo
   10. Li Min

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable and simplified classification system for predicting clinical outcomes of RPLS patients. The data were collected and analyzed using solid and validated methodology and can be used as a starting point for personalized treatment of RPLS. The work will be of interest to scientists working in the field of RPLS.

   Reviewed by eLife

3. Roles of the membrane-binding motif and the C-terminal domain of RNase E in localization and diffusion in E. coli

   This article has 8 authors:

   1. Laura Troyer
   2. Yu-Huan Wang
   3. Shobhna Shobhna
   4. Seunghyeon Kim
   5. Brooke Ramsey
   6. Jeechul Woo
   7. Emad Tajkhorshid
   8. Sangjin Kim

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study uses single-molecule imaging to characterize factors controlling the localization, mobility, and function of RNase E in E. coli, a key bacterial ribonuclease central to mRNA catabolism. The supporting evidence for the differential roles of RNAse E's membrane targeting sequence (MTS) and the C-terminal domain (CTD) to RNAse E's diffusion and membrane association is convincing. It provides insight into how RNAse E shapes the spatiotemporal organization of RNA processing in bacterial cells. This interdisciplinary work will be of interest to cell biologists, microbiologists, biochemists, and biophysicists.

   Reviewed by eLife

4. Phosphoglycerate mutase regulates Treg differentiation through control of serine synthesis and one-carbon metabolism

   This article has 11 authors:

   1. Wesley H Godfrey
   2. Judy J Lee
   3. Shruthi Shanmukha
   4. Kaho Cho
   5. Xiaojing Deng
   6. Chandra Shekar R Ambati
   7. Vasanta Putluri
   8. Abu Hena Mostafa Kamal
   9. Paul M Kim
   10. Nagireddy Putluri
   11. Michael D Kornberg

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper highlights an important physiological function of PGAM in the differentiation and suppressive activity of Treg cells by regulating serine synthesis. This role is proposed to intersect with glycolysis and one-carbon metabolism. The study's conclusion is supported by solid evidence from in-vitro cellular and in-vivo mouse models.

   Reviewed by eLife

5. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2403.16382v2

6. Citalopram exhibits immune-dependent anti-tumor effects by modulating C5aR1+ TAMs

   This article has 24 authors:

   1. Fangyuan Dong
   2. Shan Zhang
   3. Kaiyuan Song
   4. Luju Jiang
   5. Li-Peng Hu
   6. Qing Li
   7. Xue-Li Zhang
   8. Jun Li
   9. Mingxuan Feng
   10. Zhi-Wei Cai
   11. Hong-Fei Yao
   12. Rong-Kun Li
   13. Hui Li
   14. Jie Chen
   15. Xiaona Hu
   16. Jiaofeng Wang
   17. Chongyi Jiang
   18. Helen He Zhu
   19. Cun Wang
   20. Lin-Tai Da
   21. Zhi-Gang Zhang
   22. Zhijun Bao
   23. Xu Wang
   24. Shu-Heng Jiang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study provides solid evidence to support the anti-tumor potential of citalopram, originally an anti-depression drug, in hepatocellular carcinoma (HCC). In addition to their previous report on directly targeting tumor cells via glucose transporter 1 (GLUT1), the authors tried to uncover additional working mechanisms of citalopram in HCC treatment in the current study. The data here suggests that citalopram may regulate the phagocytotic function of TAM via C5aR1 or CD8+T cell function to suppress HCC growth in vivo.

   Reviewed by eLife

7. Disentangling acute motor deficits and adaptive responses evoked by the loss of cerebellar output

   This article has 6 authors:

   1. Nirvik Sinha
   2. Sharon Israely
   3. Ora Ben Harosh
   4. Ran Harel
   5. Julius PA Dewald
   6. Yifat Prut

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Using a unique cerebellar disruption approach in non-human primates, this study provides valuable new insight into how cerebellar inputs to the motor cortex contribute to reaching. The findings convincingly demonstrate that reaching movements following cerebellar disruption slow down because of both an acute deficit in producing muscle activity as well as a progressive decline in compensating for limb dynamics. This work will be of interest to neuroscientists and clinicians interested in cerebellar function and pathology.

   Reviewed by eLife

8. Antagonist actions of CMK-1/CaMKI and TAX-6/calcineurin along the C. elegans thermal avoidance circuit orchestrate adaptation of nociceptive response to repeated stimuli

   This article has 5 authors:

   1. Martina Rudgalvyte
   2. Zehan Hu
   3. Dieter Kressler
   4. Jörn Dengjel
   5. Dominique A Glauser

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study uses C. elegans to investigate how the Calcium/Calmodulin-dependent kinase CMK-1 regulates adaptation to thermo-nociceptive stimuli. The authors use compelling approaches to identify Calcineurin as a phosphorylation target of CMK-1 and to investigate the relationship between CMK-1 and Calcineurin using gain and loss of function genetic and pharmacological methods. The findings of this study are valuable as they show that CMK-1 and Calcineurin act in separate neurons in an antagonistic and complex manner to regulate thermo-nociceptive adaptation, and these results may be relevant for understanding some chronic human pain conditions.

   Reviewed by eLife

9. The second messenger signaling molecule cyclic di-AMP drives developmental cycle progression in Chlamydia trachomatis

   This article has 2 authors:

   1. Junghoon Lee
   2. Scot P Ouellette

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this useful study, ectopic expression and knockdown strategies were used to assess the effects of increasing and decreasing Cyclic di-AMP on the developmental cycle in Chlamydia. The authors convincingly demonstrate that overexpression of the dacA-ybbR operon results in increased production of c-di-AMP and early expression of the transitionary gene hctA and late gene omcB. Whilst the authors have attempted to revise the submission, the model proposed in the revised manuscript is still not fully supported by the data presented.

   Reviewed by eLife

10. Development of D-box peptides to inhibit the anaphase-promoting complex/cyclosome

    This article has 13 authors:

    1. Rohan Eapen
    2. Cynthia Okoye
    3. Christopher Stubbs
    4. Marianne Schimpl
    5. Thomas Tischer
    6. Eileen J Fisher
    7. Maria Zacharopoulou
    8. Fernando Ferrer
    9. David Barford
    10. David R Spring
    11. Catherine Lindon
    12. Christopher Phillips
    13. Laura S Itzhaki

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      The manuscript represents a fundamental advance in designing peptide inhibitors targeting Cdc20, a key activator and substrate-recognition subunit of the APC/C ubiquitin ligase. Supported by compelling biophysical and cellular evidence, the study lays a strong foundation for future developments in degron-based therapeutics. The revised manuscript has been strengthened by additional clarifications and data that address prior reviewer concerns. The work provides a robust framework for developing tools to manipulate protein degradation and will be of broad interest to researchers in protein engineering, cell cycle regulation, and targeted protein degradation.

    Reviewed by eLife