Latest preprint reviews

1. eIF3 engages with 3’-UTR termini of highly translated mRNAs

   This article has 5 authors:

   1. Santi Mestre-Fos
   2. Lucas Ferguson
   3. Marena Trinidad
   4. Nicholas T Ingolia
   5. Jamie HD Cate

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study reveals extensive binding of eukaryotic translation initiation factor 3 (eIF3) to the 3' untranslated regions (UTRs) of efficiently translated mRNAs in human pluripotent stem cell-derived neuronal progenitor cells. The authors provide solid evidence to support their conclusions, although this study may be enhanced by addressing potential biases of techniques employed to study eIF3:mRNA binding and providing additional mechanistic detail. This work will be of significant interest to researchers exploring post-transcriptional regulation of gene expression, including cellular, molecular, and developmental biologists, as well as biochemists.

   Reviewed by eLife

2. BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment

   This article has 11 authors:

   1. Emilio Rios-Jimenez
   2. Anoek Zomer
   3. Raphael Collot
   4. Mario Barrera Román
   5. Hendrikus Ariese
   6. Ravian L van Ineveld
   7. Michiel Kleinnijenhuis
   8. Nils Bessler
   9. Hannah Johnson
   10. Anne Rios
   11. Maria Alieva

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study represents a potentially useful tool for extracting quantitative data from intravital microscopy directed at in vivo cancer models. In general, this is an area of interest as accessible non-proprietary tools are needed and some evidence of the tool's utility is provided. However, the work in its current form is incomplete as it is heavily reliant on proprietary software to segment, track, and correct the data. In addition, there are significant reservations regarding the methods used to produce statistics in the software, limiting its applicability and the potential advance over other approaches.

   Reviewed by eLife

3. N6-methyladenosine in DNA promotes genome stability

   This article has 16 authors:

   1. Brooke A Conti
   2. Leo Novikov
   3. Deyan Tong
   4. Qing Xiang
   5. Savon Vigil
   6. Thomas J McLellan
   7. Chuong Nguyen
   8. Nancy De La Cruz
   9. Reshma T Veettil
   10. Prashant Pradhan
   11. Parag Sahasrabudhe
   12. Jason D Arroyo
   13. Lei Shang
   14. Benjamin R Sabari
   15. David J Shields
   16. Mariano Oppikofer

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript reports important findings that the methyltransferase METTL3 is involved in the repair of abasic sites and uracil in DNA, mediating resistance to floxuridine-driven cytotoxicity. Convincing evidence shows the involvement of m6A in DNA based on single cell imaging and mass spec data. The authors present evidence that the m6A signal does not result from bacterial contamination or RNA, but the text does not make this overly clear.

   Reviewed by eLife

4. Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation

   This article has 6 authors:

   1. Mehmet Mahsum Kaplan
   2. Erika Hudacova
   3. Miroslav Matejcek
   4. Haneen Tuaima
   5. Jan Krivanek
   6. Ondrej Machon

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this manuscript, Kaplan et al. study mesenchymal Meis2 in whisker formation and the links between whisker formation and sensory innervation. These useful findings analyze the impact of the conditional deletion of Meis2 using the Wnt1 driver on whisker development and their interaction with trigeminal nerves. The evidence supporting the conclusions is incomplete lacking mechanistic links to the phenotype described and detailed information on the methods used to analyze single-cell RNA sequencing data. The work will be of interest to developmental and skin biologists.

   Reviewed by eLife

5. Dynamics of antimicrobial resistance and virulence of Salmonella enterica serovar Dublin

   This article has 9 authors:

   1. Cheryll M Sia
   2. Rebecca L Ambrose
   3. Mary Valcanis
   4. Patiyan Andersson
   5. Susan A Ballard
   6. Benjamin P Howden
   7. Deborah A Williamson
   8. Jaclyn S Pearson
   9. Danielle J Ingle

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into the evolutionary histories and cellular infection responses of two Salmonella Dublin genotypes. While the evidence is compelling, a more phylogenetically diverse bacterial collection would enhance the findings. This research is relevant to scientists studying Salmonella and gastroenteritis-related pathogens.

   Reviewed by eLife

6. Dynamics of compartment-specific proteomic landscapes of hepatotoxic and cholestatic models of liver fibrosis

   This article has 10 authors:

   1. Marketa Jirouskova
   2. Karel Harant
   3. Pavel Cejnar
   4. Srikant Ojha
   5. Katerina Korelova
   6. Lenka Sarnova
   7. Eva Sticova
   8. Christoph H Mayr
   9. Herbert B Schiller
   10. Martin Gregor

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study suggests that the composition of the extracellular matrix in a mouse model of liver fibrosis changes depending on the cause of liver fibrosis. The data could be used as a foundation for future antifibrotic therapies. The strength of evidence is solid with respect to the use of animal models and proteomic analysis. The study provides a helpful inventory of proteins up or down-regulated, but functional analyses are limited and translational data are lacking.

   Reviewed by eLife

7. CARD8 inflammasome activation during HIV-1 cell-to-cell transmission

   This article has 3 authors:

   1. Jessie Kulsuptrakul
   2. Michael Emerman
   3. Patrick S Mitchell

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Following up on their previous work, the authors investigated whether cell-to-cell transmission of HIV-1 activates the CARD8 inflammasome in macrophages. This is important given that inflammasome activation in myeloid cells triggers proinflammatory cytokine release. The data are solid and support the idea that CARD8 is activated by the viral protease and promotes inflammation. However, time-course analyses in primary T cells and macrophages and further information on the specific inflammasome involved would further increase the significance of the study.

   Reviewed by eLife

8. Multiple Functions of Cerebello-Thalamic Neurons in Learning and Offline Consolidation of a Motor Skill in mice

   This article has 7 authors:

   1. Andres P Varani
   2. Caroline Mailhes-Hamon
   3. Romain W Sala
   4. Sarah Fouda
   5. Jimena L Frontera
   6. Clément Léna
   7. Daniela Popa

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides evidence that cerebellar projections to the thalamus are required for learning and execution of motor skills in the accelerating rotarod task. This important study adds to a growing body of literature on the interactions between the cerebellum, motor cortex, and basal ganglia during motor learning. The data presentation is generally sound, especially the main observations, with some limitations in describing the statistical methods and a lack of support for two segregated cerebello-thalamic pathways, which is incomplete in supporting the overall claim.

   Reviewed by eLife

9. Learning and cognition in a decision made at reflex speed

   This article has 3 authors:

   1. Martin Krause
   2. Wolfram Schulze
   3. Stefan Schuster

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study investigates prey capture by archer fish, showing that even though the visuomotor behavior unfolds very rapidly (within 40-70 ms), it is not hardwired; it can adapt to different simulated physics and different prey shapes. Although there was agreement that the model system, experimental design, and main hypothesis are certainly interesting, opinions were divided on whether the evidence supporting the central claims is incomplete. A more rigorous definition and assessment of "reflex speed", more detailed evidence of stimulus control, and a more detailed analysis of individual subjects could potentially increase confidence in the main conclusions.

   Reviewed by eLife

10. Parallel mechanisms signal a hierarchy of sequence structure violations in the auditory cortex

    This article has 6 authors:

    1. Sara Jamali
    2. Sophie Bagur
    3. Enora Brémont
    4. Timo Van Kerkoerle
    5. Stanislas Dehaene
    6. Brice Bathellier

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important study advances our understanding of the way neurons in the auditory cortex of mice respond to unpredictable sounds. Through the use of state-of-the-art recording methods, compelling evidence is provided that responses to local and global violations in sound sequences are prediction errors and not simply the consequence of stimulus-specific adaptation. Although the cell-type-specific results are intriguing, further work is needed to establish their reliability.

    Reviewed by eLife