eLife

Latest preprint reviews

1. Type-I nNOS neurons orchestrate cortical neural activity and vasomotion

   This article has 9 authors:

   1. Kevin Turner
   2. Dakota Brockway
   3. Md Shakhawat Hossain
   4. Keith Griffith
   5. Denver Greenawalt
   6. Qingguang Zhang
   7. Kyle Gheres
   8. Nicole Crowley
   9. Patrick J Drew

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study provides solid evidence for new insights into the role of Type-1 nNOS interneurons in driving neuronal network activity and controlling vascular network dynamics in awake, head-fixed mice. The authors use an original strategy based on the ablation of Type-1 nNOS interneurons with local injection of saporin conjugated to a substance P analogue into the somatosensory cortex. They show that ablation of type I nNOS neurons has surprisingly little effect on neurovascular coupling, although it alters neural activity and vascular dynamics.

   Reviewed by eLife

2. Site-specific DNA demethylation during spermatogenesis presets the sites of nucleosome retention in mouse sperm

   This article has 5 authors:

   1. So Maezawa
   2. Masashi Yukawa
   3. Akihiko Sakashita
   4. Artem Barski
   5. Satoshi H Namekawa

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable findings on the role of site-specific DNA methylation changes during spermatogenesis and their contribution to paternal epigenetic inheritance. The study proposes that selective loss of DNA methylation at a subset of promoters is required for nucleosome retention and the establishment of epigenetic states that may influence embryonic gene regulation. The present study's conclusion is mostly supported by solid data.

   Reviewed by eLife

3. A mathematical model clarifies the ABC Score formula used in enhancer-gene prediction

   This article has 3 authors:

   1. Joseph Nasser
   2. Kee-Myoung Nam
   3. Jeremy Gunawardena

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study dissects the mathematical and biological assumptions underlying the commonly used Activity-by-Contact model of enhancer action in transcriptional regulation. The authors provide a convincing mathematical analysis that links this (mostly phenomenological) model to concrete molecular mechanisms of enhancer function. This work provides a strong foundation from which to analyze a broad swath of genome-wide data such as that generated by CRISPRi screens.

   Reviewed by eLife

4. Structural evolution of nitrogenase over 3 billion years

   This article has 7 authors:

   1. Bruno Cuevas Zuviría
   2. Franka Detemple
   3. Kaustubh Amritkar
   4. Amanda K Garcia
   5. Lance Seefeldt
   6. Oliver Einsle
   7. Betül Kaçar

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study presents computational analyses of over 5,000 predicted extant and ancestral nitrogenase structures. The data analyses are convincing, it offers unique insights into the relationship between structural evolution and environmental and biological phenotypes. The data generated in this study provide a vast resource that can serve as a starting point for studies of reconstructed and extant nitrogenases.

   Reviewed by eLife

5. Cross-species evaluation of TANGO2 homologs, including HRG-9 and HRG-10 in Caenorhabditis elegans, challenges a proposed role in heme trafficking

   This article has 12 authors:

   1. Sarah E Sandkuhler
   2. Kayla S Youngs
   3. Olivia Gottipalli
   4. Laura D Owlett
   5. Monica B Bandora
   6. Aaliya Naaz
   7. Euri Kim
   8. Lili Wang
   9. Andrew Wojtovich
   10. Vandana Gupta
   11. Michael Sacher
   12. Samuel J Mackenzie

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study provides solid evidence that supports TANGO2 homologs, including HRG-9 and HRG-10, can play a role in cellular bioenergetics and oxidative stress homeostasis. It also challenges the previously reported role of TANGO in heme transport and paves the way for future mechanistic studies addressing the mechanisms of how TANGO2 regulates oxidative stress homeostasis. The strengths include the use of different model systems, genetic tools, behavioral assays and efforts by the authors in using the same reagents to reproduce results of other groups.

   Reviewed by eLife

6. The population structure of invasive Lantana camara is shaped by its mating system

   This article has 3 authors:

   1. P Praveen
   2. Rajesh Gopal
   3. Uma Ramakrishnan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The submission by Praveen et al. reports important findings describing the structure of genetic and colour variation in its native range for the globally invasive weed Lantana camara. Whilst the importance of the research question and the scale of the sampling is appreciated, the analysis, which is currently incomplete, requires further tests to support the claims made by the authors.

   Reviewed by eLife

7. Neocortical layer-5 tLTD relies on non-ionotropic presynaptic NMDA receptor signaling

   This article has 5 authors:

   1. Aurore Thomazeau
   2. Sabine Rannio
   3. Jennifer A Brock
   4. Hovy Ho-Wai Wong
   5. Per Jesper Sjöström

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     By using sparse Cre-dependent deletion of GluN1 subunit, in vitro quadruple patch clamp recordings, and pharmacological interventions, the authors show that spike timing dependent plasticity at between L5 synapses in the mouse visual cortex is: (i) dependent on presynaptic NMDA receptors; (ii) mediated by non-ionotropic NMDA receptor signaling, and (iii) reliant on presynaptic JNK2/Syntaxin-1a interactions. These fundamental findings advance our understanding of the molecular mechanisms underlying spike time dependent plasticity. The data are compelling and are supported by the elegant application of sophisticated experimental approaches.

   Reviewed by eLife

8. Master control genes in the regeneration of rod photoreceptors from endogenous progenitor cells in zebrafish retina

   This article has 9 authors:

   1. Eyad Shihabeddin
   2. Abirami Santhanam
   3. Stephan Tetenborg
   4. Alexandra L Aronowitz
   5. Haichao Wei
   6. Guoting Qin
   7. Chengzhi Cai
   8. Jiaqian Wu
   9. John O’Brien

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Shihabeddin et al utilized single-cell RNA-Seq analysis of adult P23H zebrafish animals to identify transcription factors (e2fs, Prdm1a, Sp1) expressed selectively in neural progenitors and immature rods, and validated their necessity for regeneration using morphant analysis. The finding is useful, and the evidence is convincing. The deeper mechanistic analysis could further strengthen the current work by (1) distinguishing developmental vs regenerative transcriptional factors, (2) the addition of matched scATAC-Seq data, and (3) integration with single-cell multiome data from developing retina.

   Reviewed by eLife

9. Sex-specific single transcript level atlas of vasopressin and its receptor (AVPR1a) in the mouse brain

   This article has 19 authors:

   1. Anisa Azatovna Gumerova
   2. Georgii Pevnev
   3. Funda Korkmaz
   4. Uliana Cheliadinova
   5. Guzel Burganova
   6. Darya Vasilyeva
   7. Liam Cullen
   8. Orly Barak
   9. Farhath Sultana
   10. Weibin Zhou
   11. Steven Lee Sims
   12. Emily Weiss
   13. Victoria Laurencin
   14. Tal Frolinger
   15. Se-Min Kim
   16. Ki A Goosens
   17. Tony Yuen
   18. Mone Zaidi
   19. Vitaly Ryu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work presents a brain-wide atlas of vasopressin (Avp) and vasopressin receptor 1A (Avpr1a) mRNA expression in mouse brains using high-resolution RNAscope in situ hybridization. The single-transcript approach provides precise localization and identifies additional brain regions expressing Avpr1a, creating a valuable resource for the field. The revised manuscript is clearer and more impactful, with improved figures, stronger data organization, and enhanced scholarship through added context and citations. Overall, the evidence is compelling, and the atlas should be broadly of use to researchers studying vasopressin signaling and related neural circuits.

   Reviewed by eLife

10. Separable dorsal raphe dopamine projections mimic the facets of a loneliness-like state

    This article has 20 authors:

    1. Christopher R Lee
    2. Gillian A Matthews
    3. Mackenzie E Lemieux
    4. Elizabeth M Wasserlein
    5. Matilde Borio
    6. Raymundo L Miranda
    7. Laurel R Keyes
    8. Gates P Schneider
    9. Caroline Jia
    10. Andrea Tran
    11. Faith Aloboudi
    12. May G Chan
    13. Enzo Peroni
    14. Grace Pereira
    15. Alba López-Moraga
    16. Anna Pallé
    17. Eyal Y Kimchi
    18. Nancy Padilla-Coreano
    19. Romy Wichmann
    20. Kay M Tye

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This study dissects the function of 3 outputs of a specific population of modulatory neurons, dorsal raphe dopamine neurons, in social and affective behavior. It provides valuable information that both confirms prior results and provides new insights. The strength of the evidence is convincing, based on cutting-edge approaches and analysis. This study will be of interest to behavioral and systems neuroscientists, especially those interested in social and emotional behavior.

    Reviewed by eLife