eLife reviews preprints in the life sciences and medicine.

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  1. Stimulating neurogenesis of distinct retinal lineages in human retinal pigmented epithelium (RPE) with proneural transcription factors

    1. L. Kaplan
    2. A.L. Green
    3. J. Pang
    4. M. Pavlou
    5. J. Wohlschlegel
    6. T.A Reh

    • Curated by eLife

      eLife Assessment

      This study provides an important contribution to retinal regeneration research by using overexpression of pro-neural factors to reprogram fetal human RPE cells into retinal neurons. The authors provide solid evidence of fetal RPE reprogramming into neural and photoreceptor-like states using scRNA-seq and imaging validation; however, there are concerns regarding comparisons between the effectiveness of different combinatorial transcription factor codes.

    Reviewed by eLife

    3 evaluationsAppears in 1 listLatest version Latest activity

  2. A nanoscale atlas of extracellular vesicles and particles in Drosophila olfactory sensilla

    1. Pawel Vijayakumar
    2. Kalyani Cauwenberghs
    3. Muskaan Garg
    4. Jonathan Choy
    5. Shadi Charara
    6. Quintyn McKaughan
    7. Chih-Ying Su

    • Curated by eLife

      eLife Assessment

      This important study presents an ultrastructural atlas of extracellular vesicles and non-vesicular particles in Drosophila olfactory sensilla, using cryofixation-based serial block-face electron microscopy to catalogue roughly 7,800 particles across four antennal regions. The evidence is compelling: the cryofixation minimizes fixation artifacts, and the rigorous, well-validated methodology and morphometric analyses provide strong support for the structural classifications and spatial distributions, though some conclusions require moderation or additional evidence. The work provides a potentially foundational resource for researchers studying extracellular-particle signaling in sensory organs.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  3. Prophase Chromosomes Relocalization to Nuclear Periphery in NPP-3/NUP205 Depletion Protects Genome Stability

    1. Ling Jiang
    2. Yu Chung Tse
    3. Karen Wing Yee Yuen

    • Curated by eLife

      eLife Assessment

      This study presents valuable findings on the functional consequences of nuclear envelope rupture caused by the depletion of nuclear pore complex subunits on the behaviour and organisation of chromosomes during mitosis in C. elegans embryos. The experiments are generally well-designed and executed; however, the evidence for some of the main conclusions is incomplete. This work is of potential interest to cell biologists working on cell division.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  5. Genome-wide mapping of Cas9-induced sister chromatid exchange across single and over 200 genomic targets

    1. Peter Chovanec
    2. Shiyang He
    3. Yi Yin

    • Curated by eLife

      eLife Assessment

      Using sci-L3-Strand-seq, this study shows genome-wide, single-cell evidence that DSBs triggered by CRISPR/Cas9 are frequently resolved through sister chromatid exchange (SCE), which is undetectable with conventional whole-genome sequencing approaches. This important work provides helpful metrics quantifying the occurrence of SCE events at targeted and repetitive genomic loci that are of particular interest to those working in gene editing, DNA repair, genome instability, or repetitive genomic loci. However, the evidence supporting the proposed involvement of under-replicated region/replication-termination-zone resolution and TRAIP/URR-like pathways is currently incomplete and could be strengthened with an increased number of reciprocal daughter-cell pairs and by genetic or molecular perturbation, or alternatively, this can be addressed by changing the discussion.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity
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