eLife

Latest preprint reviews

1. Demonstrating the ability of GABAergic cells in the zona incerta to modulate motivation

   This article has 2 authors:

   1. Laura Korobkova
   2. Brian G Dias

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This set of studies provides important knowledge for the role of zona incerta neurons in motivation and food-seeking in mice. The evidence supporting the findings is convincing, with the use of multiple approaches and nice behavioral procedures to provide converging lines of evidence. Additional analyses and data from the current studies would further strengthen the evidence. This work will be of interest to those interested in motivation and its brain substrates.

   Reviewed by eLife

2. Glycolipid MPIase is essential for the TAT (Twin-Arginine Translocation) pathway

   This article has 8 authors:

   1. Hanako Nishikawa
   2. Nachi Yamamoto
   3. Hayate Kunezaki
   4. Yui Shirogane
   5. Kotoka Kanno
   6. Katsuhiro Sawasato
   7. Miwa Yamada
   8. Ken-ichi Nishiyama

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study demonstrates a critical role of the glycolipid membrane protein insertase (MPIase) in the twin-arginine translocation (Tat) pathway, a protein translocation system that is conserved across all domains of life. The successful reconstitution of the bacterial Tat system in both bacteria-derived and artificial liposomes provides solid experimental evidence that MPIase has a broader role in membrane protein translocation than previously recognized. By revealing the essential function of a nonproteinaceous membrane component in catalyzing a core cellular process, this work offers fundamental insights into the molecular mechanisms of protein translocation.

   Reviewed by eLife

3. Quantitative multi-scale morphodynamic analysis reveals ratchet-like collective DVE migration and epiblast retrograde cell flow during anterior patterning in the mouse embryo

   This article has 10 authors:

   1. Matthew J. Stower
   2. Felix Y. Zhou
   3. Rahil Valani
   4. Jan Rozman
   5. Holly Hathrell
   6. Jonathan Godwin
   7. Xin Lu
   8. Jens Rittscher
   9. Julia M. Yeomans
   10. Shankar Srinivas

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors provide convincing evidence that, during early mouse embryogenesis, distal visceral endoderm cells migrate in a coordinated, intermittent start-and-stop manner along an axis that correlates with the asymmetric morphology of the ectoplacental cone. This fundamental finding is supported by high-quality live imaging and quantitative image analysis, while computational modelling suggests that the observed intermittent behavior may be explained by the movement of a cohesive cell group through a viscoelastic tissue, rather than by fluctuations in DVE migration velocity itself.

   Reviewed by eLife

4. A hierarchical coordinate system for sequence memory in human entorhinal cortex

   This article has 10 authors:

   1. Anna Shpektor
   2. Jacob J. W. Bakermans
   3. Alon B. Baram
   4. Johannes Sarnthein
   5. Debora Ledergerber
   6. Lukas Imbach
   7. Alina Kiseleva
   8. Emma Müller-Seydlitz
   9. Helen C. Barron
   10. Timothy E. J. Behrens

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study provides fMRI evidence for a hierarchical representation of sequence, arranged along the anterior-posterior axis of the entorhinal cortex, as well as evidence from human single-unit recordings for sequence position coding in the hippocampus and entorhinal cortex. Weaknesses include tenuous links between the single-unit and fMRI datasets, incomplete evidence for some claims, lack of discussion of relevant hippocampal literature, and potential over-interpretation of the findings in relation to rodent grid coding. This work will be of interest to researchers studying memory, sequence representations, and spatial coding in the medial temporal lobe.

   Reviewed by eLife

5. Preferential formation of NUP98-KDM5A condensates at specific H3K4me3-rich loci drives leukemogenic gene expression

   This article has 10 authors:

   1. Augusto Berrocal
   2. Jonathan E Sandoval
   3. Neha Khetan
   4. Alexa Ma
   5. Tianhong Wang
   6. Camille Moore
   7. Geeta J Narlikar
   8. Hao Li
   9. Danica Galonić Fujimori
   10. Bo Huang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is an important study establishing the mechanistic principles how NUP98-KDM5A phase separates together with H3K4me3 chromatin in leukemia. Methods are sound and results are convincing, providing a framework to understand gene expression changes in leukemia patients.

   Reviewed by eLife

6. CDK4 or CDK6 upregulation induces DNA replication stress and genomic instability to cause EGFR targeted therapy resistance in lung cancer

   This article has 15 authors:

   1. Beatrice Gini
   2. Philippe Gui
   3. Wei Wu
   4. D Lucas Kerr
   5. Lisa Tan
   6. Dora Barbosa
   7. Victor Olivas
   8. Paul Allegakoen
   9. Carlos Gomez
   10. Patrick R Halliday
   11. Sarah Elmes
   12. Veronica Steri
   13. Turja Chakrabarti
   14. Trever G Bivona
   15. Collin M Blakely

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this important study, the authors describe the mechanisms by which CDK4/6 overexpression mediates resistance to Osimertinib in EGFR mutant lung cancer models. Their data show that CD4/6 overexpressing cells had increased replicative stress and genomic instability. The evidence is solid, but inclusion of some additional experiments would make this stronger.

   Reviewed by eLife

7. The structure-interaction model of polymyxin lipopeptides with human oligopeptide transporter 2

   This article has 11 authors:

   1. Xukai Jiang
   2. Yining Luo
   3. Mohammad AK Azad
   4. Limei Xu
   5. Min Xiao
   6. Tony Velkov
   7. Kade D Roberts
   8. Visanu Thamlikitkul
   9. Qi Tony Zhou
   10. Fanfan Zhou
   11. Jian Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a potentially important structure-interaction model describing recognition of polymyxin antibiotics by the human transporter hPepT2 and applies these insights to guide the rational design of potent polymyxin B analogues with reduced nephrotoxicity. The conclusions are supported by compelling evidence, integrating molecular dynamics simulations, transporter mutagenesis, uptake assays, protein expression analyses, antibacterial testing, and mouse nephrotoxicity studies, although some mechanistic interpretations remain limited by altered transporter expression and the absence of direct structural validation.

   Reviewed by eLife

8. N6-Adenosine Methylation of a Single Site in SARS-CoV-2 5′-UTR Promotes Translation

   This article has 4 authors:

   1. Ammar Aly
   2. Gary Scott
   3. Mario Calderon
   4. A Pejmun Haghighi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study provides solid evidence that N6 methylation of A74 by METTL3 is required for efficient translation directed by the SARS-CoV-2 5′UTR in uninfected cells, likely by limiting access of protein factors to the 5′UTR that are necessary for efficient translation. These findings advance our understanding of the role of RNA modification in coronavirus replication and m6A-mediated regulation of gene expression more broadly, while further supporting METTL3 as a potential anticoronaviral therapeutic target. However, the evidence that m6A acts by destabilizing the third stem-loop (SL3) of the 5′UTR remains incomplete. The impact of the study would be strengthened by biochemical analyses directly assessing structural changes in the 5′UTR induced by A74 methylation.

   Reviewed by eLife

9. Adaptive plasticity of aspartate metabolism in succinate dehydrogenase-deficient cancer cells

   This article has 5 authors:

   1. David Sokolov
   2. Eric Zheng
   3. Serwah Danquah
   4. Madeleine L Hart
   5. Lucas B Sullivan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is a valuable study on the metabolic adaptations upon succinate dehydrogenase loss in cancer cells. If confirmed, this study will offer some therapeutic vulnerabilities in treating SDH-deficient cancer. However, the evidence supporting the authors' claim is incomplete and would benefit from additional experimental evidence. This study will be of broad interest for cancer biologists focusing on metabolism.

   Reviewed by eLife

10. Multi-area activity in mouse motor cortex associated with one- and two-handed oromanual dexterity

    This article has 4 authors:

    1. John M Barrett
    2. Joshua I Glaser
    3. Andrew Miri
    4. Gordon MG Shepherd

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This valuable study documents, for the first time, the degree of preference for contralateral versus ipsilateral forelimb movements in the motor cortex of mice, revealing subtly distinct profiles across cortical areas, with the orofacial region showing comparatively little limb selectivity relative to the forelimb motor areas. The experiments and analyses are, in general, solid and well-designed, and the data support the paper's conclusions.

    Reviewed by eLife