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  1. Microscopic PhotoSelection (MiPS) of single cells in mother machine microfluidic devices

    1. Jessica James
    2. Idris Kempf
    3. Kirill Sechkar
    4. Jingyu Wang
    5. Gabriel Abrahams
    6. Sebastian Towers
    7. Harrison Steel

    • Curated by eLife

      eLife Assessment

      The manuscript presents a compelling application of the microfluidic mother machine as a "selection" machine. While individual elements of the work have existed for many years across various research communities, the simplicity and elegance of this particular application should make it readily implementable and therefore a useful contribution. The proposed plans for revision are sound, particularly in light of the senior author's stated logistical constraints.

      [Editors' note: this paper was reviewed by Review Commons.]

    Reviewed by eLife, Review Commons

    9 evaluationsAppears in 2 listsLatest version Latest activity

  3. Dual regulation of chemical stress-induced DDI2/3 expression by a transcription factor Fzf1 and nucleosome in Saccharomyces cerevisiae

    1. Ying Du
    2. Aiyang Lin
    3. Joshua A.R. Brown
    4. LeAnn J. Howe
    5. Wei Xiao

    • Curated by eLife

      eLife Assessment

      This valuable study identifies an upstream repressive region in the Saccharomyces cerevisiae DDI2/3 promoter and shows that chemical stress induced by cyanamide or MMS is accompanied by reduced histone abundance and decreased nucleosome occupancy across the DDI2/3 locus. Genetic histone depletion is sufficient to enhance DDI2/3 expression, while MNase-seq and genetic analyses support a model in which the transcription factor Fzf1 both activates transcription through its established promoter-binding function and promotes chromatin changes that relieve nucleosome-mediated repression, helping to explain the unusually strong induction of DDI2/3 relative to other Fzf1 targets. The study is significant, as it provides a conceptually interesting extension of Fzf1-mediated stress regulation. The strength of the evidence is solid, although additional controls and a more cautious treatment of whether Fzf1 directly drives nucleosome eviction versus acting indirectly through transcriptional recruitment or chromatin-remodeling factors would further strengthen the mechanistic conclusions.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  6. Functional High Throughput Drug Screening Reveals Cyproheptadine as a Novel Treatment for LMNA -related Cardiomyopathy

    1. Hananeh Fonoudi
    2. Ali Negahi Shirazi
    3. Hui-Hsuan Kuo
    4. Carlos G. Vanoye
    5. Xiaozhi Gao
    6. Susan Doody
    7. Mariam Jouni
    8. Brian Lenny
    9. Achal Neupane
    10. Janavi Kotamarthi
    11. Yadav Sapkota
    12. Jane E. Wilcox
    13. Alfred L. George
    14. Paul W. Burridge

    • Curated by eLife

      eLife Assessment

      This study presents a valuable patient-derived human cell platform for investigating LMNA-associated cardiomyopathy and identifies cyproheptadine as a compound that improves abnormal calcium handling across multiple LMNA variants in vitro. The experimental approaches are generally rigorous and establish reproducible disease-associated cellular phenotypes; however, the evidence remains incomplete because the mechanistic basis of the observed rescue is not established, and the translational conclusions substantially exceed the presented data.

    Reviewed by eLife

    3 evaluationsAppears in 1 listLatest version Latest activity

  9. S1PR1 signaling biases neutrophils toward long-lived low-inflammatory functional states

    1. Yueh-Chien Lin
    2. Takahiro Seno
    3. Alan Y. Hsu
    4. Andréane Cartier
    5. Andrew Kuo
    6. Michel V Levesque
    7. Avishek Ghosh
    8. Ingo Fohmann
    9. Victoria A. Blaho
    10. Sylvain Galvani
    11. Reed Crocker
    12. Samuel W Kazer
    13. José Ordovás–Montañés
    14. Hongbo R. Luo
    15. Timothy Hla

    • Curated by eLife

      eLife Assessment

      This manuscript examines how overexpression of sphingosine 1-phosphate receptor 1 in a mouse model affects neutrophil distribution, metabolism and function. Given the significance of S1PR1 in inflammatory mechanisms, this work is valuable for uncovering novel aspects of S1PR1 signaling in neutrophil dynamics and function. The evidence provided is solid as it integrates single-cell transcriptomics, flow cytometry, and multiple in vivo studies. This work also raises key questions about distinct physiological and pathophysiological roles of S1PR1 signaling in neutrophils that could become therapeutic targets.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity
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