eLife

Latest preprint reviews

1. Distinct nanoscale organizations of mucins and trans -sialidases in Trypanosoma cruzi

   This article has 9 authors:

   1. Gonzalo Escalante
   2. Raquel Parada-Puig
   3. Lucía F. Lopez
   4. Alan M. Szalai
   5. María de los Milagros Cámara
   6. Luciano A. Masullo
   7. Oscar Campetella
   8. Juan Mucci
   9. Fernando D. Stefani

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study uses dual-color super-resolution microscopy, quantitative clustering analyses and simulations, as well as biochemical approaches to investigate the nanoscale organization of mucins and trans-sialidases on the surface of Trypanosoma cruzi, the causative agent of Chagas disease. The evidence supporting a non-uniform distribution of these molecules into segregated nanoclusters and a more ordered non-clustered population is solid. Overall, this work provides a generalizable analytical framework that can help understand how parasite surface proteins are spatially organized. The main remaining limitations concern the mechanistic basis of the proposed organization and the need to exclude possible effects of sample preparation before fixation.

   Reviewed by eLife

2. Ferroptosis is executed through caspase-5 cleavage of gasdermin E in ovarian cancer cells

   This article has 7 authors:

   1. Mahmuda Akter
   2. Lei Sun
   3. Cheng Chi
   4. Iram Hyder
   5. Zheng Fu
   6. Lingtao Jin
   7. Shuang Huang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     A regulated cell death pathway that intentionally causes ferroptosis has not been previously described. The authors describe a useful finding that the ferroptosis inducers ML162 and erastin induce caspase-5-mediated cleavage of GSDME in mesenchymal-like ovarian cancer cells, resulting in pore formation. The data supporting the main claims are incomplete. This work will be of interest to researchers who study regulated cell death.

   Reviewed by eLife

3. Measurement and comparison of acoustic space use in vocalizations of humans and close primate relatives

   This article has 3 authors:

   1. Hans T. Bilger
   2. Michael J. Ryan
   3. Julia A. Clarke

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an important empirical analysis of the acoustic space of vocal repertoires across primates and humans, providing new data that challenge the widely held hypothesis that the expansion of the human vocal space, driven by modifications of the vocal tract, was a key prerequisite for the evolution of spoken language. The evidence is convincing in its technical implementation and acoustic measurements, offering helpful comparative insights into voiced vocalizations. However, the theoretical framing and interpretation would benefit from further discussion.

   Reviewed by eLife

4. Super compensatory substitutions restore protein function and flatten fitness landscapes

   This article has 5 authors:

   1. Wenhong Jiang
   2. Hengchuang Yin
   3. Saad S. Khan
   4. Yiparemu Abuduhaibaier
   5. Lucas B. Carey

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study identifies and characterizes a set of amino acid states that can rescue protein function in the presence of substantially deleterious mutations. Some of these super-compensatory substitutions also confer substantial mutational robustness, with broader implications for understanding epistasis, protein evolution, and protein engineering. The evidence is convincing, supported by a creative reanalysis of a large deep-mutational-scanning dataset, statistically rigorous treatment of anticipated error rates, experimental validation, and analyses of additional proteins, although clearer presentation and deeper investigation of the evolutionary implications and structural mechanisms would further strengthen the study.

   Reviewed by eLife

5. High basal autophagic activity in the brain revealed by systemic quantitative analysis using GFP-LC3-RFP mice

   This article has 7 authors:

   1. Yuki Kanda
   2. Tomoya Eguchi
   3. Hideaki Morishita
   4. Yutaro Hama
   5. Manabu Abe
   6. Kenji Sakimura
   7. Noboru Mizushima

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The ability to measure autophagic flux in vivo in response to physiological stresses remains a challenge for investigators in the field; the novel mouse model present in this work is significant and should prove valuable to investigators in addressing shortcomings of existing models. In addition, the development of the microplate reader approach to permit semi-high-throughput analysis of samples is convincing and a significant advance. The application of these approaches to measuring autophagy in multiple tissues is appreciated but raises some questions that need to be answered and highlighted, including what new biological insight has been generated for tissues under study, how overall autophagy versus rates of flux are determined, and how the sex of the animal affects outcomes. The neuronal populations under study should be reassessed.

   Reviewed by eLife

6. Cell cycle-coupled CK1δ turnover, autoinhibition, and activity

   This article has 4 authors:

   1. Fidel E. Serrano
   2. Bianca Ruppert
   3. Axel C. R. Diernfellner
   4. Michael Brunner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript presents valuable experimental results describing the localisation and regulation of casein kinase CK1δ during the cell cycle. During the G2 phase of the cell cycle, the autophosphorylation of the C-terminal tail stabilises and inhibits the kinase which might protect CK1δ in the subsequent G1 phase. The results may be of interest to researchers working on casein kinase function and regulation but due to lack of some controls remain incomplete.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife

7. Life-stage-specific specialities in the cell atlases of the Clytia hemisphaerica planula and medusa

   This article has 14 authors:

   1. Anna Ferraioli
   2. Julia Ramon Mateu
   3. Marc Meynadier
   4. Thomas Lamonerie
   5. Sophie Pagnotta
   6. Sandra Chevalier
   7. Marta Iglesias
   8. Sebastian R Najle
   9. Arnau Sebé-Pedrós
   10. Marie-Jeanne Arguel
   11. Julie Cazareth
   12. Virginie Magnone
   13. Evelyn Houliston
   14. Richard R. Copley

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides a useful single-cell atlas of the Clytia hemisphaerica planula, complemented by an updated medusa dataset, ultrastructural analyses and in situ validation of expression patterns. The cross-stage comparison and cluster-similarity framework offer a promising basis for investigating cellular diversification across the life cycle. The evidence has the potential to be convincing, but is currently incomplete due to documentation deficits and insufficient cross-referencing with prior work. It should be relatively easy to address these issues, which should make this a valued resource for cnidarian researchers and for colleagues studying cell-type evolution.

   Reviewed by eLife

8. We couldn't get details of this article at this time:
   10.64898/2026.05.01.722137

9. We couldn't get details of this article at this time:
   10.1101/2025.09.09.675276

10. We couldn't get details of this article at this time:
    10.64898/2026.05.28.728318