eLife

Latest preprint reviews

1. White Matter Stratification in Depression Predicts Multidimensional Antidepressant Responses

   This article has 11 authors:

   1. Jiaolong Qin
   2. Xinyi Wang
   3. Huangjing Ni
   4. Ye Wu
   5. Haiyan Liu
   6. Lingling Hua
   7. Rui Yan
   8. Hao Tang
   9. Peng Zhao
   10. Zhijian Yao
   11. Qing Lu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents valuable findings for identifying biotypes of depression patients using white matter measures, which are under-utilised and under-appreciated in current biological and computational psychiatry work. The evidence supporting the claims is solid, although enhanced interpretability of the identified biotypes across both white matter and symptom levels, and better justification of the choice of models would strengthen the paper. Overall, this study will be of interest to the broad community of neuroimagers, clinicians, and biological and computational psychiatry researchers.

   Reviewed by eLife

2. Systematic yeast two-hybrid screening identifies novel functions for SET1C/COMPASS

   This article has 15 authors:

   1. Pierre Luciano
   2. Kihyun Park
   3. Stéphane Audebert
   4. Luc Camoin
   5. Carlos A. Niño
   6. Da Kyeong Park
   7. Isabella E. Maudlin
   8. Marion Dubarry
   9. Lara Lee
   10. Marlene Oeffinger
   11. Jean D. Beggs
   12. Young Hye Kim
   13. Jaehoon Kim
   14. Bernhard Dichtl
   15. Vincent Géli

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study uses the yeast two-hybrid assay to identify proteins that may interact with yeast Set1 and other subunits of COMPASS/Set1C, the histone H3K4 methyltransferase, providing also some evidence for Set1 sumoylation and a role of SET1C methylating other factors in vitro. The results are valuable, and they should contribute to understanding the functions of the conserved SET1C complex, as they suggest potential functional connections with RNA biogenesis, chromatin remodeling, and non-histone methylation, whose implications would yet need to be explored. Nevertheless, apart from the fact that only a small subset of the Y2H interactions is further examined, the validating experiments are only partial or inconclusive, the strength of evidence being at this point incomplete.

   Reviewed by eLife

3. Proteomic composition and mutual assembly of the C2a projection in vertebrate motile cilia

   This article has 11 authors:

   1. Qian Lyu
   2. Qingchao Li
   3. Jingrui Li
   4. Jiajun Luo
   5. Chunyu Liu
   6. Shanshan Nai
   7. Hongbin Liu
   8. Xueliang Zhu
   9. Ting Song
   10. Min Liu
   11. Huijie Zhao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into the protein composition of the C2a projection in mouse motile cilia, building upon prior work in Chlamydomonas. The evidence supporting the claims of the authors is solid. The work will be of interest to biologists and clinicians studying cilia and ciliopathies.

   Reviewed by eLife

4. Primordial cardiomyocytes orchestrate myocardial morphogenesis and vascularization but are dispensable for regeneration

   This article has 3 authors:

   1. Jisheng Sun
   2. Lu Chen
   3. Jinhu Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work identifies phlda2 as a specific marker for primordial cardiomyocytes in the adult zebrafish heart and demonstrates their essential role in myocardial morphogenesis and coronary vascularization, but not in heart regeneration. The conclusions are well supported by single-cell transcriptomics, new genetic tools, and cell-specific ablation experiments. Overall, the evidence is solid and provides insight into the difference between developmental and regenerative cardiac programs. This work will be of interest for those studying cardiac development and regeneration.

   Reviewed by eLife

5. Alternative splicing of PIF4 regulates plant development under heat stress

   This article has 6 authors:

   1. María Niño-González
   2. Benjamin Alary
   3. Dóra Szakonyi
   4. Tom Laloum
   5. Paula Duque
   6. Guiomar Martín

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript identifies temperature-dependent alternative splicing of PIF4 in Arabidopsis thaliana and shows that heat stress promotes the accumulation of a short exon 5-skipping isoform that is predicted to encode a non-functional protein. This finding is important, and it provides an intriguing new layer of regulation for PIF4; however, the strength of the mechanistic conclusions is limited, and several key conclusions rely on indirect evidence. As a result, while the data robustly demonstrate heat-regulated alternative splicing of PIF4, the causal role of PIF4 isoforms' balance in shaping heat-induced developmental responses remains only partially supported. This work will be of interest to biologists working on alternative splicing.

   Reviewed by eLife

6. Deep mutational scanning reveals pharmacologically relevant insights into TYK2 signaling and disease

   This article has 24 authors:

   1. Conor J Howard
   2. Nathan S Abell
   3. Robert R Warneford-Thomson
   4. Eden Mahdavi
   5. Alan L Su
   6. Carmen Resnick
   7. Nabil Mohammed
   8. Erin M Thompson
   9. Emily R Holzinger
   10. Katrina Catalano
   11. Abhay Hukku
   12. Gabriel A Mintier
   13. Morgan MacKenzie
   14. Bryan L Jiang
   15. Dora Barbosa Rabago
   16. Angela Chan
   17. Carolindah Ntimi
   18. Kaitlyn N Weiler
   19. Stephen C Wilson
   20. Joseph C Maranville
   21. Payal R Sheth
   22. Robert M Plenge
   23. Sriram Kosuri
   24. Diane E Dickel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important paper presents a rigorous and comprehensive deep mutational analysis of the kinase TYK2, revealing how single amino acid substitutions influence protein abundance, signaling activity, and responses to pharmacological inhibitors. By combining high‑quality experimental design with dose‑response signaling assays and multiple inhibitor conditions, the authors generate a robust dataset that identifies variants across all domains of TYK2, including clusters at functionally critical sites and protein-protein interfaces. The study highlights mutations that drive drug resistance or potentiation and shows that reduced TYK2 abundance aligns with protective autoimmune‑associated variants, underscoring the therapeutic relevance of modulating TYK2 stability. Overall, the work provides compelling insights with clear implications for biochemistry, immunology, clinical genetics, and drug discovery.

   Reviewed by eLife

7. Flexible and high-throughput simultaneous profiling of gene expression and chromatin accessibility in single cells

   This article has 5 authors:

   1. Volker Soltys
   2. Moritz Peters
   3. Dingwen Su
   4. Marek Kučka
   5. Yingguang Frank Chan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a useful methodological advance that better enables the simultaneous measurement of gene expression and chromatin accessibility in individual cells. The evidence supporting the improved detection of gene expression is solid, though the reduced performance in detecting chromatin accessibility represents a limitation. This method will be of interest to those studying transcription and gene regulation.

   Reviewed by eLife

8. Temporal single-cell analysis reveals age-associated delay in immune resolution after respiratory viral infection

   This article has 17 authors:

   1. Yue Wu
   2. Chaofan Li
   3. Jinyi Tang
   4. Xiaochen Gao
   5. In Su Cheon
   6. Bibo Zhu
   7. Ruixuan Zhang
   8. Cori E. Fain
   9. Shengen Shawn Hu
   10. Harish Narasimhan
   11. Gislane de Almeida Santos
   12. Katayoun Ayasoufi
   13. Aaron J. Johnson
   14. Hui Zong
   15. Chongzhi Zang
   16. Haidong Dong
   17. Jie Sun

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents valuable findings that could potentially allow a deeper understanding of the immunopathogenesis underlying influenza infection in aged mice. The results are based on solid evidence that define putative immune determinants underlying immunopathology in the aged lung. This study will be of interest to researchers pursuing aging research, as well as to immunologists.

   Reviewed by eLife

9. A high-throughput assay for the measurement of Ca2+-oscillations and insulin release from uniformly sized β-cell spheroids

   This article has 4 authors:

   1. Stijn Robben
   2. Patricia Davidson
   3. Rita S Rodrigues Ribeiro
   4. Thomas Voets

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this valuable study, Robben et al. describe a 3D beta-cell spheroid platform, a tool allowing high-throughput monitoring of cytoplasmic calcium concentrations and insulin secretion, with calcium signals comparable to those recorded in primary pancreatic islets. The authors demonstrate the method by culturing MIN6 cells in a 3D culture system, and show solid evidence of its utility by recording calcium signals in a high-throughput format and characterizing these calcium signals using pharmacological tools. This highlights the potential utility of the 3D beta-cell spheroids for screening new pharmacological modulators of pancreatic beta-cell function.

   Reviewed by eLife

10. Contractile peri-nuclear actomyosin network repositions peripheral and polar chromosomes to promote early kinetochore–microtubule interactions

    This article has 6 authors:

    1. Nooshin Sheidaei
    2. John K Eykelenboom
    3. Zuojun Yue
    4. Graeme Ball
    5. Alexander JR Booth
    6. Tomoyuki U Tanaka

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important study demonstrates that a peri-nuclear actomyosin network, present in some types of human cells, facilitates kinetochore-spindle attachment of chromosomes in unfavorable locations - thereby suppressing their missegregation rate. This actomyosin network and its general role have been studied previously, but this study convincingly clarifies the underlying mechanism using a light-controlled perturbation and detailed tracking of kinetochore movement. The generality of the mechanism could be further supported by confirming the findings in non-synchronized cells and additional cell lines. The results may have implications for understanding chromosome missegregation in cancer cells.

      [Editors' note: this paper was reviewed by Review Commons.]

    Reviewed by eLife, Review Commons