eLife

Latest preprint reviews

1. Three Dimensional Dynamics of Epithelial Monolayers

   This article has 4 authors:

   1. Silja Borring Låstad
   2. Nigar Abbasova
   3. Thomas Combriat
   4. Dag Kristian Dysthe

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This article by Lästad and colleagues proposes to reconsider some important aspects of single-cell volume and dry mass homeostasis in a model system of epithelial monolayer, based on a novel method relying on quantitative phase imaging to measure these parameters. The article proposes an in-depth analysis of a large dataset uniquely based on this method. This is the main current weakness of the manuscript, since the claims that differ from previous findings are not tested using other methods to exclude potential artifacts.

   Reviewed by eLife

2. Single-cell characterization of tumor immune landscapes in colorectal cancer humanized mice

   This article has 7 authors:

   1. Sanne Bootsma
   2. Job Saris
   3. Andrew Y.F. Li Yim
   4. Kristiaan J. Lenos
   5. Felipe A. Vieira Braga
   6. Joep Grootjans
   7. Louis Vermeulen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this study, Bootsma and colleagues use single-cell RNA-seq to characterize immune cell populations in a colorectal human mouse model. Because these models have not yet been fully characterized from this standpoint, the data generated will be useful to the field. The study is technically solid and lays the foundation for subsequent mechanistic and functional work.

   Reviewed by eLife

3. Genome-wide structural variant landscape following HDR-enhanced CRISPR editing in human hematopoietic stem and progenitor cells

   This article has 5 authors:

   1. Fanny-Meï Cloarec-Ung
   2. Guy Sauvageau
   3. Hilary M Sheppard
   4. Peter M. Lansdorp
   5. David JHF Knapp

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into the structural consequences of CRISPR-Cas9 editing in human hematopoietic stem and progenitor cells, identifying large deletions and genome-wide structural variants using nanopore sequencing and Strand-seq, while demonstrating PolQ inhibition as a potential strategy to reduce large deletions. Although the evidence is generally solid and supports the conclusions, several aspects remain incomplete. In particular, further validation of genome-wide structural alterations, larger single-cell datasets, and functional studies would strengthen the findings and their implications for gene therapy safety and DNA repair mechanisms.

   Reviewed by eLife

4. Single-molecule behavior and cell-growth regulation in human RTKs

   This article has 6 authors:

   1. Mitsuhiro Abe
   2. Masataka Yanagawa
   3. Yotaro Koizumi
   4. Ryoji Kise
   5. Asuka Inoue
   6. Yasushi Sako

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study measures how 52 of the 58 human receptor tyrosine kinases move in the membrane of living cells, one molecule at a time, before and after stimulation, and provides a comparative resource for the field. The evidence for these measurements is solid: the raw movies and tracking results are publicly deposited, and openly available code reproduces the analysis of movement states on a representative subset, although the tracking step relies on commercial software. The evidence linking these movement patterns to the regulation of cell growth is incomplete, because it rests on correlations with public datasets the authors did not test experimentally. The work will interest cell biologists and biophysicists who study membrane receptors and signalling.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

5. Metabolically Activated Proteostasis Regulators Reduce Differentiation of CD4+ TH17 Cells

   This article has 7 authors:

   1. Priyadarshini Chatterjee
   2. Keishla Sánchez Ortiz
   3. Stanislav Dikiy
   4. Michael J Bollong
   5. Jessica E Thaxton
   6. Alejandra Mendoza
   7. R Luke Wiseman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable evidence that the metabolically activated proteostasis regulator AA147 preferentially suppresses T helper 17 cell differentiation, implicating nuclear factor erythroid 2-related factor 2 (NRF2), reduced reactive oxygen species (ROS) levels, and proteasome-dependent degradation of RORγt in this effect. The findings are supported by solid evidence from complementary pharmacological, genetic, biochemical, transcriptomic, proteomic, and cellular approaches. However, the precise mechanistic link between NRF2 activation, ROS reduction, and RORγt degradation remains to be fully elucidated.

   Reviewed by eLife

6. A structured-illumination miniscope for optically sectioned imaging and real-time neural decoding

   This article has 6 authors:

   1. Huixin Lin
   2. Shu Wang
   3. Yuhang Zhu
   4. Zhaoyang Yin
   5. Qingchun Guo
   6. Jingfeng Zhou

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study describes a well-designed lightweight head-mounted microscope that uses structured illumination to suppress out-of-focus background fluorescence, potentially enhancing real-time decoding of neuronal activity. However, the evidence for its central performance claims is incomplete: whereas the method shows improvement compared to unprocessed conventional images, typical image processing steps widely used in the field generated similar results, suggesting a limited practical advance.

   Reviewed by eLife

7. Longitudinal analysis of visuomotor orientation after optic lobe lesions reveals brain plasticity in Drosophila

   This article has 3 authors:

   1. Margarida Caio
   2. Dean Rance
   3. Christa Rhiner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study uses longitudinal single-animal tracking in the Buridan arena to examine recovery of visually guided behavior after optic lobe injury in Drosophila and identifies a potential role for Myc-dependent neural progenitor activation in this process. The evidence is incomplete, as key controls and analyses are lacking to exclude alternative explanations for the behavioral recovery and to firmly establish the requirement for progenitor activation.

   Reviewed by eLife

8. Interocular suppression weakens binocular disparity integration in the dorsal visual stream

   This article has 2 authors:

   1. Rong Jiang
   2. Ming Meng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study uses multi-session fMRI and multivariate analyses to investigate how interocular suppression affects binocular disparity representations across the human visual cortex. The authors provide convincing evidence that interocular suppression weakens disparity-related information in the dorsal visual stream, particularly in V3A and IPS. The findings further suggest that suppression alters anticorrelated binocular disparity representations and reduces coordinated disparity-related information across dorsal visual areas. Together, these results advance our understanding of how binocular integration and interocular suppression interact and highlight the involvement of higher-level dorsal visual regions in these processes.

   Reviewed by eLife

9. Healed predation scar on a Cambrian apex predator

   This article has 5 authors:

   1. Kun-sheng Du
   2. Yu Wang
   3. Juan Gao
   4. Stephen Pates
   5. Wei Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this useful study, Du and colleagues report a fossil specimen of a swimming flap probably belonging to the radiodont Amplectobelua symbrachiata from a new locality of the early Cambrian Chengjiang Biota. The authors interpret a W-shaped notch as the first evidence of healed injuries on an apex predator of the Cambrian. With this reasonable and solid evidence, the manuscript has potential significance for understanding ecological interactions among high trophic levels in the Cambrian.

   Reviewed by eLife

10. In vivo optical clearing of the mouse brain

    This article has 8 authors:

    1. Maiko Kume
    2. Ningdong Kang
    3. Alejandro Akrouh
    4. Dae Woo Kim
    5. Joshua T Dearborn
    6. David F Wozniak
    7. Daniel Kerschensteiner
    8. Timothy E Holy

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      An in vivo imaging medium raises the refractive index of the fluid surrounding cells in the brain, rendering living tissue more accessible to light for in vivo experimentation. This important approach enables imaging neurons more brightly and at greater depth than in untreated tissue. Solid evidence, consisting of both optical and electrophysiological neural activity recordings in brain slices and in living mice, demonstrate a clearing effect, albeit subtle, and not without a significant impact on mice behavior, which limits the use of the proposed method. This work will be of relevance to any optical studies of in vivo tissue.

    Reviewed by eLife