eLife reviews preprints in the life sciences and medicine.

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  1. The microtubule-associated proteins CKAP2 and its paralog CKAP2- Like control ciliogenesis in human cells

    1. Luiz E. da Silva
    2. Gabriel Campolina-Silva
    3. Jenine Afani
    4. Clémence Belleannée
    5. Susanne Bechstedt

    • Curated by eLife

      eLife Assessment

      In this valuable study, the authors build on previous work demonstrating that the microtubule-associated protein CKAP2 functions at the mitotic spindle to regulate microtubule dynamics for proper chromosome segregation. Here, the authors show that CKAP2 and its paralog CKAP2L also function at the cilium to regulate cilium formation and Hedgehog signaling. The writing is clear, the experiments and data are generally convincing, and the proper data analysis is carried out; however, the evidence supporting the proposal that CKAP2 and CKAP2L compensate for each other is incomplete. Overall, the findings advance the field in our knowledge of microtubule-associated proteins with roles in both the mitotic spindle and the cilium.

    Reviewed by eLife

    3 evaluationsAppears in 1 listLatest version Latest activity

  2. Cross-Kingdom Control: Yeast Prion Protein [ MRPL10 + ] Modulates Host Physiology in Drosophila

    1. Chih-Chun Janet Lin
    2. Jessica Y. Jiang
    3. Maushmi D. Chitale
    4. Elissa J. Cosgrove
    5. Alexandria Van Elgort
    6. Asha M. Jain
    7. Julia C. Kelso
    8. Xinyue Cui
    9. Nilay Yapici
    10. Andrew G. Clark

    • Curated by eLife

      eLife Assessment

      This valuable study shows first that yeast strains potentially associated with the [MRPL10+] prion state strongly influence Drosophila cold tolerance and locomotor behavior, and it goes on to exploit natural variation to identify host pathways contributing to these responses. The evidence for host physiology and genetics is solid, but the evidence for MRPL10 prion causality is incomplete because the prion state has not been directly validated in the yeast strains used. The study would be substantially strengthened either by establishing the presence of the MRPL10 prion state, or by tempering the prion-specific causal claims and framing involvement of MPRL10 or other prions in the yeast strains as a plausible but unproven mechanism. The work will be of interest to many fields, including quantitative genetics, insect physiology and microbe-host interactions.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  3. Fitness flux in SARS-CoV-2 and influenza H3N2

    1. Trevor Bedford

    • Curated by eLife

      eLife Assessment

      This valuable study presents a comparative framework for quantifying adaptation in SARS-CoV-2 and influenza H3N2 from changes in clade frequencies, enabling comparison of rates of adaptation across the two viruses on a common per-generation scale and showing that a simple count of mutations performs as well as much more sophisticated protein language models in predicting variant fitness. The evidence supporting the quantitative estimates of fitness flux is currently incomplete because the analysis assumes a single, time-independent fitness for each clade; for antigenically evolving viruses, clade fitness changes with population immunity, and it remains unclear how this assumption affects the estimated fitness flux. The interpretation of cumulative fitness flux in relation to changes in absolute fitness over time also requires clarification.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  4. Glia regulate local retinoic acid levels to specify neuronal specialisation for high-acuity vision

    1. Manuela Lahne
    2. Roxana Lungu
    3. Madison Snorton
    4. Takeshi Yoshimatsu
    5. Ryan B. MacDonald

    • Curated by eLife

      eLife Assessment

      This important study investigates how retinoic acid signaling and retinal support cells called Müller glia might contribute to regional differences in the morphology and function of cone photoreceptors - light-sensitive neurons in the zebrafish retina - and may have broader implications for understanding how non-neuronal cells such as glia shape neuronal cell specialization in other regions of the nervous system. Despite well-executed imaging and pharmacological experiments, the evidence remains incomplete because the proposed mechanism is not established as specific to Müller glia, and broader effects on retinal development, cell growth, maturation, and/or differentiation could explain the observed shortening of light-sensitive structures. The work will interest researchers studying vision, nervous system development, and interactions between neurons and their supporting cells.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  8. A Sterol-Binding Cavity Underlies Sterol Recognition and Differential Activation of the ABCG5/G8

    1. Fatemeh Rezaei
    2. Isra F. Omar
    3. Danny Farhat
    4. Zi-Wen Weng
    5. Kadambari Vijay Sai
    6. Qing-Fang Xiao
    7. Yuan-Chih Chang
    8. Shang-Te Danny Hsu
    9. Jyh-Yeuan Lee

    • Curated by eLife

      eLife Assessment

      This work presents valuable findings that identify ergosterol binding by the human ABC transporter ABCG5/G8 and show that ergosterol stimulates its ATPase activity more strongly than cholesterol, advancing understanding of sterol recognition and transport. The evidence from single-particle cryo-EM, molecular dynamics simulations, and biochemical assays is convincing, although additional structures and mutational studies would strengthen the proposed mechanism. These results provide key insight into the molecular basis of sterol recognition and regulation of ABCG5/G8 and will be of interest to researchers studying sterol transport and ABC transporter function.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity
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