eLife

Latest preprint reviews

1. Distinct allosteric remodeling of HIV-1 Env dynamics on virions by gp41-directed antibodies reveals two modes of neutralization

   This article has 15 authors:

   1. Wang Xu
   2. Narendra Kumar Gonepudi
   3. Junyu Liu
   4. Yufan He
   5. Revansiddha Katte
   6. Ran Wang
   7. Harry Baffour Awuah
   8. Yang Han
   9. Baoshan Zhang
   10. Jian Yu
   11. Bo Hu
   12. David D. Ho
   13. Priyamvada Acharya
   14. Peter D. Kwong
   15. Maolin Lu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript reports an important study in which the authors apply smFRET imaging to probe HIV-1 Env conformational dynamics in the presence of antibodies. Previous implementations of smFRET imaging of HIV-1 Env, which focus on gp120 conformation, have yielded limited information on antibodies that target gp41. Through the cutting-edge application of smFRET imaging, the study provides convincing insights into the mechanisms of action of relevant antibodies.

   Reviewed by eLife

2. Cellular basis of accelerated whole-tooth regeneration

   This article has 5 authors:

   1. Talha Mubeen
   2. Haowen He
   3. George W. Gruenhagen
   4. Anoushka Satoskar
   5. Jeffrey T. Streelman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into the cellular dynamics underlying accelerated tooth regeneration in a vertebrate model. Using single-nucleus RNA sequencing across multiple time points, the authors present a well-structured analysis of cell populations, trajectories, and intercellular signaling events associated with this process. The strength of evidence is solid but incomplete, as the conclusions are primarily supported by computational inference, without experimental validation of key findings.

   Reviewed by eLife

3. Gelsolin Counteracts ER Stress-Driven Inflammatory Circuits in Psoriasis-like Dermatitis

   This article has 20 authors:

   1. Daisuke Ori
   2. Haruna Okude
   3. Riko Konishi
   4. Motoya Murase
   5. Shuya Hiroki
   6. Saki Takahara
   7. Towa Tanaka
   8. Rina Toyodome
   9. Norisuke Kano
   10. Takumi Kawasaki
   11. Ken J Ishii
   12. Kouji Kobiyama
   13. Hideyuki Nakashima
   14. Kinichi Nakashima
   15. Miwa Sasai
   16. Masahiro Yamamoto
   17. Yutaro Kumagai
   18. Akio Tsuru
   19. Kenji Kohno
   20. Taro Kawai

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper provides a valuable observation that imiquimod, a compound often used to induce a psoriasis-like skin inflammation in mice, has a TLR7-independent effect acting through the unfolded protein response and binding to Gelsolin. However, the mechanism connecting Gelsolin to skin inflammation presented in this paper is incomplete and requires further investigation. These findings are of interest to the field of skin immunology.

   Reviewed by eLife

4. Beyond the Focus of Expansion: Retinal curl as a functional signal for heading estimation

   This article has 2 authors:

   1. Kontessa I Zorpala
   2. Joan López-Moliner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides an important and biologically plausible account of how human perceptual judgments of heading direction are influenced by a specific pattern of motion in optic flow fields known as retinal curl. By combining psychophysical experiments and neural modeling, the authors demonstrate that what was previously considered an incidental "nuisance" signal actually serves as a functional control signal for estimating heading and steering toward a fixated target. While the evidence for the role of curl signals is convincing and advances our understanding of vision-based navigation, the work's impact would be strengthened by situating these findings among other cues that contribute to heading estimation, and by clarifying both the time course of these computations and their generalizability across different navigational contexts.

   Reviewed by eLife

5. The overall and sequence-specific degradation of soil extracellular 16S rRNA genes across China: rates and influential factors

   This article has 9 authors:

   1. Ting Li
   2. Song Zhang
   3. Zelin Wang
   4. Wei Huang
   5. Zejin Zhang
   6. Fang Wang
   7. Dong Liu
   8. Xiaoyong Cui
   9. Rongxiao Che

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study introduces an innovative experimental design to address a crucial and timely issue in microbial ecology: the potential bias in soil microbial community analyses caused by extracellular DNA degradation. While the evidence showing variable degradation rates of extracellular DNA is convincing, additional conceptual, methodological, and statistical clarifications could reinforce the claims and the study's contribution to the field. This research will appeal to microbial ecologists and researchers interested in using molecular techniques to evaluate microbial community structure.

   Reviewed by eLife

6. Long-range neural pathways for octopus chemotactile processing revealed from periphery-to-brain by centimeter-field microCT

   This article has 15 authors:

   1. Andrew Sugarman
   2. Daniel Vanselow
   3. David Northover
   4. Stephen L Senft
   5. Carolyn Zaino
   6. Maksim A Yakovlev
   7. Jessica Christ
   8. Justin D Silverman
   9. Mee S Ngu
   10. Khai C Ang
   11. Steve Wang
   12. Wen-Sung Chung
   13. Patrick La Riviere
   14. Roger T Hanlon
   15. Keith C Cheng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this study, the authors use microCT to image an intact hatchling octopus and segment major organ systems, including the vascular, respiratory, digestive, and nervous systems. The resulting dataset is of good quality, and its release through a public web interface is a valuable resource for the community to explore cephalopod mesoscale anatomy. However, the authors claim to have elucidated previously uncharacterized chemotactile pathways from the suckers to the brain, for which there is incomplete evidence, as microCT does not reveal structural connectivity. In addition, the language is often overly complex, obscuring the main points and making it difficult to assess the strength of individual claims. This article would benefit from more cautious framing of the anatomical findings and complementary neuronal tracing experiments to support the proposed pathways.

   Reviewed by eLife

7. Gene specificity landscapes for comparative transcriptomic analysis across tissues, cell types, and species

   This article has 2 authors:

   1. Erik Bot
   2. Jose Davila-Velderrain

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study advances a new computational approach to measure and visualize gene expression specificity across different tissues and cell types. The framework is potentially helpful for improving the way gene expression specificity is defined across biological datasets, especially among single-cell datasets. The evidence supporting the method is generally solid, although further evaluation of the method's robustness and comparison to other approaches would strengthen the conclusions.

   Reviewed by eLife

8. Five-layer systems analysis of Leishmania stage differentiation reveals an essential role for protein degradation in parasite development

   This article has 16 authors:

   1. Pascale Pescher
   2. Thibaut Douché
   3. Quentin Giai-Gianetto
   4. Karen Druart
   5. Julie Kovarova
   6. Blaise Li
   7. Thomas Cokelaer
   8. K Shanmugha Rajan
   9. Laura Piel
   10. Céline Besse
   11. Anne Boland
   12. Jean-François Deleuze
   13. Mariette Matondo
   14. Michael P Barrett
   15. Shulamit Michaeli
   16. Gerald F Späth

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study provides a comprehensive multi-omics characterization of Leishmania donovani stage differentiation, offering insights into the molecular basis of parasite adaptation across host environments. The authors present convincing evidence that stage transitions are not driven by genomic variation but instead rely on coordinated post-transcriptional regulation, including mRNA turnover, translation, and protein degradation. Although experimental validation of these findings and conclusions remains to be completed, the integration of diverse, high-quality datasets establishes a robust resource that will be of broad utility to researchers investigating Leishmania biology and life-cycle progression.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

9. HIV-1 Envelope glycoprotein modulates CXCR4 clustering and dynamics on the T cell membrane

   This article has 16 authors:

   1. Adriana Quijada-Freire
   2. César A Santiago
   3. Eva M García-Cuesta
   4. Blanca Soler-Palacios
   5. Rosa Ayala-Bueno
   6. Sofía R Gardeta
   7. Enara San Sebastian
   8. Eva Armendariz-Burgoa
   9. María C Puertas
   10. Ricardo Villares
   11. Urtzi Garaigorta
   12. Luis Ignacio González-Granado
   13. José Miguel Rodríguez-Frade
   14. Jakub Chojnacki
   15. Javier Martinez-Picado
   16. Mario Mellado

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into how HIV-1 Env modulates the nanoscale organization and dynamics of the CXCR4 co-receptor on T cells, using quantitative imaging and functional approaches, the authors present convincing evidence that gp120 engagement promotes CD4-dependent clustering and altered mobility of CXCR4, distinct from the effects of the natural ligand CXCL12. Some concerns were raised regarding the interpretation of the single-particle tracking analyses, and additional clarification or analysis may help strengthen the conclusions. The physiological relevance of the findings could be further enhanced by validation with infectious virus and by more clearly integrating the CXCR4R334X mutant observations into the central mechanistic narrative. The work will be of interest to researchers studying HIV entry and membrane receptor organization.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

10. Serotonergic modulation of motor subspace dynamics drives a sleep-independent quiescent state

    This article has 5 authors:

    1. Kexin Qi
    2. Yuming Chai
    3. Guodong Tan
    4. Daguang Li
    5. Quan Wen

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      In light of the diverse functions associated with the Dorsal Raphe Nucleus across vertebrate species, this important study presents findings on the role of serotonin in promoting behavioral quiescence through the regulation of neuromotor populations. Combining optogenetics with brain-wide activity analyses, the study provides convincing evidence of interest to researchers in neuromodulation and translational medicine fields.

    Reviewed by eLife