eLife

Latest preprint reviews

1. Hugin-AstA circuitry is a novel central energy sensor that directly regulates sweet sensation in Drosophila and mouse

   This article has 6 authors:

   1. Wusa Qin
   2. Tingting Song
   3. Zeliang Lai
   4. Daihan Li
   5. Liming Wang
   6. Rui Huang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work characterizes layers of neuropeptidergic modulations that collectively regulate the intake of sugar in a hunger state-dependent manner. Combinations of genetic, physiological, and behavioral approaches present convincing evidence that neurons that release Hugin and Allatostatin A are in an active state in sated flies, leading to suppression of sugar feeding behavior by reducing the sensitivity of sugar-sensitive gustatory neurons that express Gr5a. They also demonstrate that neurons that release Neuromedin U, a vertebrate homolog of Hugin, have common physiological properties as the fly Hugin neurons, revealing a similar function of evolutionarily conserved peptides across animal phyla.

   Reviewed by eLife

2. Regulation of sensorimotor serial learning in speech production by motor compensation rather than sensory error

   This article has 6 authors:

   1. Yuhan Lu
   2. Xiaowei Tang
   3. Zhenyan Xiao
   4. Anqi Xu
   5. Junxi Chen
   6. Xing Tian

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study investigates how people adapt their speech when auditory feedback is altered. The analyses are rigorous and the work makes a valuable contribution by extending methods from limb motor control to speech. However, because the paradigm does not directly measure sensory error, the evidence for the proposed mechanism of sensorimotor learning is incomplete. The findings are best viewed as evidence for how prior motor adjustments influence subsequent behaviour, highlighting the need for future studies to more precisely separate sensory and motor contributions to adaptation.

   Reviewed by eLife

3. A comprehensive mechanosensory connectome reveals a somatotopically organized neural circuit architecture controlling stimulus-aimed grooming of the Drosophila head

   This article has 5 authors:

   1. Steven A. Calle-Schuler
   2. Alexis E. Santana-Cruz
   3. Lucia Kmecová
   4. Stefanie Hampel
   5. Andrew M. Seeds

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study extends prior work on head bristle mechanosensation by delivering a synaptic-resolution map of second-order partners that preserves somatotopy and highlights a cholinergic pathway linking sensory input to grooming circuits, providing a valuable resource for the field. The reconstructions and quantitative connectivity analyses provide solid support the main anatomical claims, while causal sufficiency for the behavioral sequence remains inferential and could be strengthened by a simple rank-order test relating wiring to the known grooming hierarchy.

   Reviewed by eLife

4. Direct contact between iPSC-derived macrophages and hepatocytes drives reciprocal acquisition of Kupffer cell identity and hepatocyte maturation

   This article has 13 authors:

   1. Christopher Zhe Wei Lee
   2. Farah Tasnim
   3. Xiaozhong Huang
   4. Raman Sethi
   5. Yoohyun Song
   6. Tatsuya Kozaki
   7. Sebastiaan De Schepper
   8. Nicholas Ang
   9. Ivy Low
   10. You Yi Hwang
   11. Jinmiao Chen
   12. Hanry Yu
   13. Florent Ginhoux

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This timely and fundamental study introduces a human iPSC-based co-culture system that models Kupffer cell-hepatocyte interactions and aims to recapitulate liver-specific immune-parenchymal dynamics. Direct contact between iMacs and iHeps promotes mutual tissue-specific maturation, with iHeps downregulating fetal genes while iMacs acquire a Kupffer cell-like profile. This convincing in vitro model holds significant promise and is a leap forward; future experimental understanding will enhance its translational impact.

   Reviewed by eLife

5. Early exercise disrupts a pro-repair extracellular matrix program during zebrafish fin regeneration

   This article has 5 authors:

   1. Victor M. Lewis
   2. Rafael A. Fernandez
   3. Samuel G. Horst
   4. Carlos A. Gonzalez
   5. Kryn Stankunas

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The results in this study are useful because they begin to establish a causal link between physical activity and the cellular mechanisms of regeneration. The evidence presented is largely solid, supporting the conclusion that exercise-induced changes in the extracellular matrix disrupt regeneration; however, some claims are incomplete, requiring additional controls and a clearer distinction between the effects of mechanical loading and mechanical injury to the blastema. The work will be of interest to researchers in regenerative medicine.

   Reviewed by eLife

6. Rejuvenation-Responsive and Senolytic-Sensitive Muscle Stem Cells Unveiled by CD200 and CD63 in Geriatric Muscle

   This article has 13 authors:

   1. Ye Lynne Kim
   2. Young-Woo Jo
   3. Takwon Yoo
   4. Kyusang Yoo
   5. Ji-Hoon Kim
   6. Myungsun Park
   7. In-Wook Song
   8. Hyun Kim
   9. Yea-Eun Kim
   10. Sang-Hyeon Hann
   11. Jong-Eun Park
   12. Daehyun Baek
   13. Young-Yun Kong

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study is useful because it challenges the widely accepted notion that muscle stem cell numbers decline with aging, providing novel insights into population heterogeneity and the identification of new surface markers for geriatric MuSCs. However, the evidence is considered incomplete due to insufficient quantitative comparisons of absolute cell numbers, limited analysis of age groups (particularly the lack of "aged" mice as opposed to geriatric), and the need for further functional and mechanistic validation of key subpopulations. Additional concerns that require clarification include the lack of statistical rigor in some experiments, the presentation of supporting data not being complete, and the overextension of claims relating to senescence and new marker validation. Overall, while the findings advance understanding of MuSC aging, the conclusions drawn by the authors should be strengthened with expanded experiments and more rigorous data analysis.

   Reviewed by eLife

7. Contrasting roles for IKK regulated inflammatory signalling pathways for development and maintenance of type 1 and adaptive γδ T cells

   This article has 6 authors:

   1. Farjana Islam
   2. Cayman Williams
   3. Thea Hogan
   4. Louise Webb
   5. Ines Boal-Carvalho
   6. Benedict Seddon

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into the role of the NF-kB and IKK signaling pathways in γδ T cell development and survival, using robust genetic mouse models. While the research is methodologically sound, some conclusions require further evidence, with incomplete analyses, particularly regarding cell-intrinsic effects and mechanistic details. Overall, the findings are significant for immunologists interested in innate-like T cell biology and advancing the understanding of γδ T cell differentiation and maintenance.

   Reviewed by eLife

8. Long-range inhibitory axons from medial entorhinal cortex target lateral entorhinal neurons projecting to the hippocampal formation

   This article has 5 authors:

   1. Eirik S. Nilssen
   2. Bente Jacobsen
   3. Thanh P. Doan
   4. Paulo J. B. Girão
   5. Menno P. Witter

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides a valuable insight into how the medial and lateral entorhinal cortices interact through distinct excitatory and inhibitory pathways. Using anatomical tracing, optogenetics, and electrophysiology, the authors show that glutamatergic medial entorhinal neurons provide broad excitatory input to lateral entorhinal, while long-range SST+ interneurons deliver selective inhibition to layer I. These findings reveal a novel layer- and cell-type-specific organization of medial to lateral entorhinal connectivity with implications for spatial and episodic memory. The work is solid, but validation of injection specificity and viral spread is needed to fully confirm the anatomical interpretations; with these clarifications, this will be a significant contribution to understanding entorhinal-hippocampal circuit organization.

   Reviewed by eLife

9. DNA tensiometer reveals catch-bond detachment kinetics of kinesin-1, -2 and -3

   This article has 5 authors:

   1. Crystal R Noell
   2. Tzu-Chen Ma
   3. Rui Jiang
   4. Scott A McKinley
   5. William O Hancock

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The use of DNA tethers is an important advance for studying how motor proteins respond to load. The authors use a convincing methodology to investigate the detachment and reattachment kinetics of kinesin-1, 2, and 3 motors against loads oriented parallel to the microtubule. As the manuscript stands, the conclusions drawn from the experiments, as well as the overall interpretation of the results, are incompletely supported by the presented data, and the novelty over previous reports appears less clear.

   Reviewed by eLife

10. NAD+ boosting by oral nicotinamide mononucleotide administration regulates key metabolic and immune pathways through SIRT1 dependent and independent mechanisms to mitigate diet-induced obesity and dyslipidemia in mice

    This article has 12 authors:

    1. Yasser Majeed
    2. Najeeb M Halabi
    3. Rudolf Engelke
    4. Hina Sarwath
    5. Muna N Al-Noubi
    6. Sunkyu Choi
    7. Aisha Al-Malki
    8. Maha V Agha
    9. Muneera Vakayil
    10. Lotfi Chouchane
    11. Frank Schmidt
    12. Nayef A Mazloum

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      The mechanistic basis for the potential health benefits of NAD⁺ precursors remains incompletely understood. This manuscript provides a useful assessment of the role of SIRT1 in mediating the effects of NMN in mice fed a high-fat diet. The study addresses a key question, though some of the conclusions appear only partially supported by the presented data.

    Reviewed by eLife