Synthetic Biology

Enhanced Cas12a multi-gene regulation using a CRISPR array separator

1. Jens P Magnusson
2. Antonio Ray Rios
3. Lingling Wu
4. Lei S Qi

• Curated by eLife

  Evaluation Summary:

  This manuscript is of broad interest to those performing multiplexed genome engineering and related applications with CRISPR Cas12a technologies. While the proposed use of synSeparators is promising, the paper would benefit from further investigation of the mechanism by which synSeparators function to promote Cas12a activity. Additional data would be required to support the current conclusions regarding the generalizability of the findings.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Mapping the Functional Landscape of the Receptor Binding Domain of T7 Bacteriophage by Deep Mutational Scanning

1. Phil Huss
2. Anthony Meger
3. Megan Leander
4. Kyle Nishikawa
5. Srivatsan Raman

Reviewed by eLife

Tetravalent SARS-CoV-2 Neutralizing Antibodies Show Enhanced Potency and Resistance to Escape Mutations

1. Shane Miersch
2. Zhijie Li
3. Reza Saberianfar
4. Mart Ustav
5. James Brett Case
6. Levi Blazer
7. Chao Chen
8. Wei Ye
9. Alevtina Pavlenco
10. Maryna Gorelik
11. Julia Garcia Perez
12. Suryasree Subramania
13. Serena Singh
14. Lynda Ploder
15. Safder Ganaie
16. Rita E. Chen
17. Daisy W. Leung
18. Pier Paolo Pandolfi
19. Giuseppe Novelli
20. Giulia Matusali
21. Francesca Colavita
22. Maria R. Capobianchi
23. Suresh Jain
24. J.B. Gupta
25. Gaya K. Amarasinghe
26. Michael S. Diamond
27. James Rini
28. Sachdev S. Sidhu

Reviewed by PREreview, ScreenIT

Converting endogenous genes of the malaria mosquito into simple non-autonomous gene drives for population replacement

1. Astrid Hoermann
2. Sofia Tapanelli
3. Paolo Capriotti
4. Ellen K. G. Masters
5. Tibebu Habtewold
6. George K. Christophides
7. Nikolai Windbichler

Reviewed by eLife

A massively parallel reporter assay library to screen short synthetic promoters in mammalian cells

1. Adam M. Zahm
2. William S. Owens
3. Samuel R. Himes
4. Braden S. Fallon
5. Kathleen E. Rondem
6. Alexa N. Gormick
7. Joshua S. Bloom
8. Sriram Kosuri
9. Henry Chan
10. Justin G. English

Reviewed by preLights

Massively parallel protein-protein interaction measurement by sequencing (MP3-seq) enables rapid screening of protein heterodimers

1. Alexander Baryshev
2. Alyssa La Fleur
3. Benjamin Groves
4. Cirstyn Michel
5. David Baker
6. Ajasja Ljubetič
7. Georg Seelig

Reviewed by preLights

Quantifying the antimicrobial activity of CRISPR-Cas9-accA modified Δ B. subtilis mutants against V. harveyi and E. Coli

1. Tatiana Hillman

Reviewed by PREreview

Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast

1. Yue Shen
2. Feng Gao
3. Yun Wang
4. Yuerong Wang
5. Ju Zheng
6. Jianhui Gong
7. Jintao Zhang
8. Zhouqing Luo
9. Daniel Schindler
10. Yang Deng
11. Weichao Ding
12. Tao Lin
13. Reem Swidah
14. Hongcui Zhao
15. Shuangying Jiang
16. Cheng Zeng
17. Shihong Chen
18. Tai Chen
19. Yong Wang
20. Yisha Luo
21. Leslie Mitchell
22. Joel S. Bader
23. Guojie Zhang
24. Xia Shen
25. Jian Wang
26. Xian Fu
27. Junbiao Dai
28. Jef D. Boeke
29. Huanming Yang
30. Xun Xu
31. Yizhi Cai

Reviewed by preLights

Agrochemical control of gene expression using evolved split RNA polymerase

1. Yuan Yuan
2. Jin Miao

Reviewed by PeerJ

Periodic spatial patterning with a single morphogen

1. Sheng Wang
2. Jordi Garcia-Ojalvo
3. Michael B. Elowitz

Reviewed by preLights