1. Loss of CTRP10 results in female obesity with preserved metabolic health

    This article has 7 authors:
    1. Fangluo Chen
    2. Dylan C Sarver
    3. Muzna Saqib
    4. Leandro M Velez
    5. Susan Aja
    6. Marcus M Seldin
    7. G William Wong
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This manuscript presents a detailed characterization of male and female wildtype and Ctrp10 knockout mice, and reveals that knockout mice develop female-specific obesity that is largely uncoupled from metabolic dysfunction. The data are convincing, and the work will be an important contribution to understanding how obesity is coupled to metabolic dysfunction, and how this can occur in a sex-specific manner.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  2. Megakaryocytes assemble a three-dimensional cage of extracellular matrix that controls their maturation and anchoring to the vascular niche

    This article has 17 authors:
    1. Claire Masson
    2. Cyril Scandola
    3. Jean-Yves Rinckel
    4. Fabienne Proamer
    5. Emily Janus-Bell
    6. Fareeha Batool
    7. Naël Osmani
    8. Jacky G Goetz
    9. Léa Mallo
    10. Catherine Léon
    11. Alicia Bornert
    12. Renaud Poincloux
    13. Olivier Destaing
    14. Alma Mansson
    15. Hong Qian
    16. Maxime Lehmann
    17. Anita Eckly
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The authors provide a thorough investigation of the interaction of megakaryocytes (MK) with their associated extracellular matrix (ECM) during maturation; they provide evidence that the existence of a dense cage-like pericellular structure containing laminin γ1 and α4 and collagen IV is key to fixing the perisinusoidal localization of MK and preventing their premature intravasation. Adhesion of MK to this ECM cage is dependent on integrin beta1 and beta3 expressed by MK. This strong and solid conclusion is based on the use of state-of-the art techniques such as the use of primary murine bone marrow MK cultures, mice lacking ECM receptors, namely integrin beta1 and beta3 null mice, as well as high-resolution 2D and 3D imaging. The study provides valuable insight into the role of cell-matrix interactions in MK maturation and provides an interesting model with practical implications for the fields of hemostasis and thrombosis.

    Reviewed by preLights, eLife

    This article has 6 evaluationsAppears in 2 listsLatest version Latest activity
  3. Adiponectin exerts sex-dependent effects on lipid, amino acid, and glucose metabolism during caloric restriction

    This article has 17 authors:
    1. Yoshiko M Ikushima
    2. Kuan-Chan Chen
    3. Richard J. Sulston
    4. Domenico Mattiucci
    5. Eleanor J. Brain
    6. Stefanie A Fung Xin Zi
    7. Karla J. Suchacki
    8. Benjamin J. Thomas
    9. Andrea Lovdel
    10. Matthew Bennett
    11. Hiroshi Kobayashi
    12. Phillip D. Whitfield
    13. Keiyo Takubo
    14. Andrew H. Baker
    15. Nicholas M. Morton
    16. Robert K. Semple
    17. William P. Cawthorn

    Reviewed by PREreview

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  4. The Kv2.2 channel mediates the inhibition of prostaglandin E2 on glucose-stimulated insulin secretion in pancreatic β-cells

    This article has 12 authors:
    1. Chengfang Pan
    2. Ying Liu
    3. Liangya Wang
    4. Wen-Yong Fan
    5. Yunzhi Ni
    6. Xuefeng Zhang
    7. Di Wu
    8. Chenyang Li
    9. Jin Li
    10. Zhaoyang Li
    11. Rui Liu
    12. Changlong Hu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The study presents valuable findings on the molecular mechanisms of glucose-stimulated insulin secretion from pancreatic islets, focusing on the main regulatory elements of the signaling pathway in physiological conditions. While the evidence supporting the conclusions is solid, the study can be strengthened by the use of a beta cell line or knockout mice. The work will be of interest to cell biologists and biochemists working on diabetes.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  5. Blood pressure variability compromises vascular function in middle-aged mice

    This article has 8 authors:
    1. Perenkita J Mendiola
    2. Philip O’Herron
    3. Kun Xie
    4. Michael W Brands
    5. Weston Bush
    6. Rachel E Patterson
    7. Valeria Di Stefano
    8. Jessica A Filosa
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This is an important study providing convincing evidence that increased blood pressure variability impairs myogenic tone and diminishes baroreceptor reflex. The study also provides evidence that blood pressure variability blunts functional hyperemia and contributes to cognitive decline. The authors use appropriate and validated methodology in line with the current state-of-the-art.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  6. Neuronal Detection Triggers Systemic Digestive Shutdown in Response to Adverse Food Sources

    This article has 8 authors:
    1. Yating Liu
    2. Guojing Tian
    3. Ziyi Wang
    4. Junkang Zheng
    5. Huimin Liu
    6. Sucheng Zhu
    7. Zhao Shan
    8. Bin Qi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study investigates how signals from the nervous system can influence the response to different food sources. To demonstrate the role of specific neuronal and intestinal regulators in sensing food quality and modulating digestion, the authors present evidence through a combination of genetic screening, RNA-seq analysis, and functional studies. While the findings shed light on an adaptive strategy to integrate food perception with physiological responses, the evidence presented varies between convincing and incomplete, and additional experiments are needed to more fully support their central hypothesis.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  7. TRPV4 overactivation enhances cellular contractility and drives ocular hypertension in TGFβ2 overexpressing eyes

    This article has 10 authors:
    1. Christopher N Rudzitis
    2. Monika Lakk
    3. Ayushi Singh
    4. Sarah N Redmon
    5. Denisa Kirdajova
    6. Yun-Ting Tseng
    7. Michael L De Ieso
    8. W Daniel Stamer
    9. Samuel Herberg
    10. David Križaj
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This fundamental work extends our understanding of the role of TGFβ2 as a modulator of mechanosensing in the eye and identifies the TRPV4 ion channel as a common regulator of Trabecular Meshwork (TM) contractility and pathological OHT. The data and evidence are convincing, with some minor limitations. This work will clearly be of interest to researchers investigating the role of mechanosensors in the TM and may underpin future research into treatments that aim to lower intra ocular pressure. This work will additionally be of interest to the growing field of researchers investigating the regulation of force sensing via ion channels and their roles in health and disease, in particular the ion channel TRPV4.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  8. Characterization of the cellular components of mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells regulating lymphatic contractions

    This article has 12 authors:
    1. SD Zawieja
    2. GA Pea
    3. SE Broyhill
    4. A Patro
    5. KH Bromert
    6. CE Norton
    7. HJ Kim
    8. SK Sivasankaran
    9. M Li
    10. JA Castorena-Gonzalez
    11. BT Drumm
    12. MJ Davis
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This manuscript aims to identify the pacemaker cells in the lymphatic collecting vessels - the cells that initiate the autonomous action potentials and contractions needed to drive lymphatic pumping. Through the exemplary use of existing approaches (genetic deletions and cytosolic calcium detection in multiple cell types), the authors convincingly determine that lymphatic muscle cells are the origin of the action potential that triggers lymphatic contraction. The inclusion of scRNAseq and membrane potential data enhances a tremendous study. This fundamental discovery establishes a new standard for the field of lymphatic physiology.

    Reviewed by eLife

    This article has 13 evaluationsAppears in 1 listLatest version Latest activity
  9. Peripheral opioid receptor antagonism alleviates fentanyl-induced cardiorespiratory depression and is devoid of aversive behavior

    This article has 11 authors:
    1. Brian C. Ruyle
    2. Sarah Masud
    3. Rohith Kesaraju
    4. Mubariz Tahirkheli
    5. Juhi Modh
    6. Caroline G. Roth
    7. Sofia Angulo-Lopera
    8. Tania Lintz
    9. Jessica A. Higginbotham
    10. Nicolas Massaly
    11. Jose A. Moron
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This manuscript represents a fundamental contribution demonstrating that fentanyl-induced respiratory depression can be reversed with a peripherally-restricted mu opioid receptor antagonist. The paper reports compelling and rigorous physiological, pharmacokinetic, and behavioral evidence supporting this major claim, and furthers mechanistic understanding of how peripheral opioid receptors contribute to respiratory depression. These findings reshape our understanding of opioid-related effects on respiration and have significant therapeutic implications given that medications currently used to reverse opioid overdose (such as naloxone) produce severe aversive and withdrawal effects via actions within the central nervous system.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  10. Multi-dimensional oscillatory activity of mouse GnRH neurons in vivo

    This article has 5 authors:
    1. Su Young Han
    2. Shel-Hwa Yeo
    3. Jae-Chang Kim
    4. Ziyue Zhou
    5. Allan E Herbison
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study investigates the oscillatory activity of gonadotropin-releasing hormone (GnRH) neurones in mice using GCaMP fiber photometry. It demonstrates three distinct patterns of oscillatory activity that occur in GnRH neurons comprising low-level rapid baseline activity, abrupt short-duration oscillations that drive pulsatile gonadotropin secretion, and, in females, a gradual and prolonged oscillating increase in activity responsible for the relatively short-lived preovulatory LH surge. The evidence presented in the study is solid, offering theoretical implications for understanding the behaviour of GnRH neurones in the context of reproductive physiology, and will be of interest to researchers in neuroendocrinology and reproductive biology.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
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