Physiology

RiboTRAP-seq identifies spatially distinct functions for the anterior and posterior intestine in immune and metabolic regulation in C. elegans

1. Chung-Chih Liu
2. Nicolas Seban
3. Supriya Srinivasan

• Curated by eLife

  eLife Assessment

  In this valuable study, the authors performed cell-specific ribosome pulldown to identify gene expression (translatome) differences in the anterior (NT1) vs middle & posterior (NT2-9) cells of the C. elegans intestine, under fed, starved, or refeeding conditions. The data generated will be very helpful to the C. elegans community, and the evidence supporting the conclusions of the study is assessed to be solid. Some methodological caveats remain and are discussed.

Reviewed by eLife

The myocyte Nfe2l1-ubiquitin-proteasome system controls muscle fiber type and obesity-induced insulin resistance

1. Imke L. Lemmer
2. Daniel T. Haas
3. Fernando Rivera
4. Stefan Kotschi
5. Leonardo Matta
6. Nienke Willemsen
7. Carolin Jethwa
8. Joel Guerra
9. Alba Mena Gómez
10. Irmak Toksöz
11. Ejona Gjika
12. Sajjad Khani
13. Maria Rohm
14. Nick Diercksen
15. Phong B.H. Nguyen
16. Michael P. Menden
17. Desalegn T. Egu
18. Jens Waschke
19. Steen Larsen
20. Tao Ma
21. Zachary Gerhart-Hines
22. Stephan Herzig
23. Kenneth Dyar
24. Natalie Krahmer
25. Alexander Bartelt

Reviewed by Review Commons

Sex-biased expression of enteroendocrine cell-derived hormones contributes to higher fat storage in Drosophila females

1. Puja Biswas
2. Elizabeth J Rideout

• Curated by eLife

  eLife Assessment

  This useful study provides a systematic comparison of sex-biased enteroendocrine hormone expression in Drosophila and suggests that gut-derived peptides may contribute to female-biased triglyceride levels. The revised manuscript includes helpful textual clarifications and an integrative model, but the evidence remains incomplete, because the proposed role of Tk is still over-interpreted relative to authors' stated criterion for statistical significance against both parental controls. The work will be of interest to researchers studying sex differences in metabolism, but the central mechanistic claims require either stronger experimental support or more careful qualification.

Reviewed by eLife