Pathology

Cbfβ regulates Wnt/β-catenin, Hippo/Yap, and Tgfβ signaling pathways in articular cartilage homeostasis and protects from ACLT surgery-induced osteoarthritis

1. Wei Chen
2. Yun Lu
3. Yan Zhang
4. Jinjin Wu
5. Abigail McVicar
6. Yilin Chen
7. Siyu Zhu
8. Guochun Zhu
9. You Lu
10. Jiayang Zhang
11. Matthew McConnell
12. Yi-Ping Li

• Curated by eLife

  eLife assessment

  This fundamental work advances our understanding of the role of Cbfβ in maintaining articular cartilage homeostasis and the underlying mechanisms. The evidence supporting the conclusion is mostly convincing, although including additional experiments and discussions would have strengthened the study. This paper is of potential interest to skeletal biologists and orthopaedic surgeons who study the pathogenesis and the therapeutics of osteoarthritis.

Reviewed by eLife

Membrane-bound O-acyltransferase 7 (MBOAT7) shapes lysosomal lipid homeostasis and function to control alcohol-associated liver injury

1. Venkateshwari Varadharajan
2. Iyappan Ramachandiran
3. William J Massey
4. Raghav Jain
5. Rakhee Banerjee
6. Anthony J Horak
7. Megan R McMullen
8. Emily Huang
9. Annette Bellar
10. Shuhui W Lorkowski
11. Kailash Gulshan
12. Robert N Helsley
13. Isabella James
14. Vai Pathak
15. Jaividhya Dasarathy
16. Nicole Welch
17. Srinivasan Dasarathy
18. David Streem
19. Ofer Reizes
20. Daniela S Allende
21. Jonathan D Smith
22. Judith Simcox
23. Laura E Nagy
24. J Mark Brown

• Curated by eLife

  eLife assessment

  Varadharajan et al. explore the mechanistic basis of MBOAT7 SNP association with steatotic liver disease and link its function in LPI acylation to altered lipidomics of endosomal/lysosomal system and impaired TFEB mediated lysosomal biogenesis. The findings are important with theoretical and practical implications in MAFLD, alcohol-induced hepatic steatosis, and lysosomal diseases. The strength of evidence is convincing using methodology in line with current state-of-the-art.

Reviewed by eLife

Open-top Bessel beam two-photon light sheet microscopy for three-dimensional pathology

1. Won Yeong Park
2. Jieun Yun
3. Jinho Shin
4. Byung Ho Oh
5. Gilsuk Yoon
6. Seung-Mo Hong
7. Ki Hean Kim

• Curated by eLife

  eLife assessment

  This important work by Park et al. demonstrates an open-top two-photon light sheet microscopy (OT-TP-LSM) for lesser invasive evaluation of intraoperative 3D pathology. The authors provide convincing evidence for the effectiveness of this technique investigating various human cancer cells. This article will be of broad interest to biologists and, specifically, pathologists utilizing 3D optical microscopy.

Reviewed by eLife

The intricate relationship of G-Quadruplexes and bacterial pathogenicity islands

1. Bo Lyu
2. Qisheng Song

• Curated by eLife

  eLife assessment

  This fundamental study explores the relationship between guanine-quadruplex structures and pathogenicity islands in 89 bacterial strains representing a range of pathogens. Guanine-quadruplex structures were found to be non-randomly distributed within pathogenicity islands and conserved within the same strains. These compelling findings shed light on the molecular mechanisms of Guanine-quadruplex structure-pathogenicity island interactions and will be of interest to all microbiologists.

Reviewed by eLife

β-catenin inhibition disrupts the homeostasis of osteogenic/adipogenic differentiation leading to the development of glucocorticoid-induced osteonecrosis of the femoral head

1. Chenjie Xia
2. Huihui Xu
3. Liang Fang
4. Jiali Chen
5. Wenhua Yuan
6. Danqing Fu
7. Xucheng Wang
8. Bangjian He
9. Luwei Xiao
10. Chengliang Wu
11. Peijian Tong
12. Di Chen
13. Pinger Wang
14. Hongting Jin

• Curated by eLife

  eLife assessment

  This study presents valuable findings on the mechanism of glucocorticoid-induced osteonecrosis of the femoral head. The data were collected and analyzed using solid, validated methodology and can be used as a starting point for functional studies of development of glucocorticoid-induced osteonecrosis. This paper would be of interest to cell biologists and biophysicists working on potential pharmacological treatments for glucocorticoid-induced osteonecrosis.

Reviewed by eLife

Activin A marks a novel progenitor cell population during fracture healing and reveals a therapeutic strategy

1. Lutian Yao
2. Jiawei Lu
3. Leilei Zhong
4. Yulong Wei
5. Tao Gui
6. Luqiang Wang
7. Jaimo Ahn
8. Joel D Boerckel
9. Danielle Rux
10. Christina Mundy
11. Ling Qin
12. Maurizio Pacifici

• Curated by eLife

  eLife assessment

  This work is a valuable presentation of sharp-wave-ripple reactivation of hippocampal neural ensemble activity recorded as animals explored two different environments. It attempts to use the fact that the ensemble code remaps between the two mazes to identify the best replay-detection procedures for analyzing this type of data. The reviewers found the evidence for a prescriptive conclusion inadequate, while still appreciating the concept of comparing maze-identity discrimination with replay.

Reviewed by eLife

A novel sustained release therapy of combined VEGF and TNF-α inhibitors leads to pan-ocular protection for months after severe ocular trauma

1. Chengxin Zhou
2. Fengyang Lei
3. Pui-Chuen Hui
4. Natalie Wolkow
5. Claes H. Dohlman
6. Demetrios G. Vavvas
7. James Chodosh
8. Eleftherios I. Paschalis

Reviewed by PREreview

Mouse gingival single-cell transcriptomic atlas identified a novel fibroblast subpopulation activated to guide oral barrier immunity in periodontitis

1. Takeru Kondo
2. Annie Gleason
3. Hiroko Okawa
4. Akishige Hokugo
5. Ichiro Nishimura

• Curated by eLife

  eLife assessment

  The findings of this article provide valuable information on the changes of cell clusters induced by chronic periodontitis. The observation of a new fibroblast subpopulation, named AG fibroblasts, is interesting, and the strength of evidence presented is solid.

Reviewed by eLife

Iron chelation improves ineffective erythropoiesis and iron overload in myelodysplastic syndrome mice

1. Wenbin An
2. Maria Feola
3. Maayan Levy
4. Srinivas Aluri
5. Marc Ruiz-Martinez
6. Ashwin Sridharan
7. Eitan Fibach
8. Xiaofan Zhu
9. Amit Verma
10. Yelena Ginzburg

• Curated by eLife

  eLife assessment

  This study presents a valuable finding that erythrocyte precursors could re-gain EPO responsiveness after DFP chelation therapy. In addition, the authors investigated iron trafficking in erythroblasts using the MDS mouse model. However, the evidence supporting the claims of the authors is still inadequate. The work will be of interest to medical biologists working on hematology.

Reviewed by eLife

VEGFA mRNA-LNP promotes biliary epithelial cell-to-hepatocyte conversion in acute and chronic liver diseases and reverses steatosis and fibrosis

1. Fatima Rizvi
2. Yu-Ri Lee
3. Ricardo Diaz-Aragon
4. Pushpinder S. Bawa
5. Juhoon So
6. Rodrigo M. Florentino
7. Susan Wu
8. Arianna Sarjoo
9. Emily Truong
10. Anna R. Smith
11. Feiya Wang
12. Elissa Everton
13. Alina Ostrowska
14. Kyounghwa Jung
15. Ying Tam
16. Hiromi Muramatsu
17. Norbert Pardi
18. Drew Weissman
19. Alejandro Soto-Gutierrez
20. Donghun Shin
21. Valerie Gouon-Evans

Reviewed by preLights