Neuroscience

Parkinson’s disease-associated Pink1 loss disrupts vesicle trafficking in Ensheathing glia causing dopaminergic neuron synapse loss

1. Lorenzo Ghezzi
2. Ulrike Pech
3. Nils Schoovaerts
4. Suresh Poovathingal
5. Kristofer Davie
6. Jochen Lamote
7. Roman Praschberger
8. Patrik Verstreken

• Curated by eLife

  eLife Assessment

  The preliminary data presented in this manuscript seem valuable. The data are currently incomplete and there are numerous technical concerns, some of which may arise from insufficient description of methodologies used.

Reviewed by eLife

Strikingly different neurotransmitter release strategies in dopaminergic subclasses

1. Ana Dorrego-Rivas
2. Darren J Byrne
3. Yunyi Liu
4. Menghon Cheah
5. Ceren Arslan
6. Marcela Lipovsek
7. Marc C Ford
8. Matthew S Grubb

• Curated by eLife

  eLife Assessment

  This study presents solid evidence for distinct neurotransmitter release modalities between two subclasses of dopaminergic neurons in the olfactory bulb, highlighting an important finding that dendritic neurotransmitter release in anaxonic neurons and axonal neurotransmitter release in axon-bearing neurons, and GABAergic self-inhibition in anaxonic neurons emphasizes the functional differences between these neuronal subtypes. However, some experiments looked incomplete with a relatively small sample size (low n). The conclusion would benefit significantly from additional validations.

Reviewed by eLife

Mesoscale functional architecture in medial posterior parietal cortex

1. Riichiro Hira
2. Leah B Townsend
3. Ikuko T Smith
4. Che-Hang Yu
5. Jeffrey N Stirman
6. Yiyi Yu
7. Spencer LaVere Smith

• Curated by eLife

  eLife Assessment

  This important study measures the functional specialization of distinct subregions within the mouse posterior parietal cortex (PPC) using mesoscopic two-photon calcium imaging during visual discrimination and choice history-dependent tasks. It presents compelling evidence supporting the existence of functional specialized subregions within the PPC. The work will be of interest to system and computational neuroscientists interested in decision-making, working memory, and multisensory integration.

Reviewed by eLife

Translatome analysis reveals cellular network in DLK-dependent hippocampal glutamatergic neuron degeneration

1. Erin M Ritchie
2. Dilan Acar
3. Siming Zhong
4. Qianyi Pu
5. Yunbo Li
6. Binhai Zheng
7. Yishi Jin

• Curated by eLife

  eLife Assessment

  This manuscript describes the impact of modulating signaling by a key regulatory enzyme, Dual Leucine Zipper Kinase (DLK), on hippocampal neurons. The results are interesting and will be important for scientists interested in synapse formation, axon specification, and cell death. The authors have carefully addressed the comments made by the reviewers and the findings are convincing in large part due to the use of extensive mouse genetics, detailed gene expression of enriched genes, and recognition of neuron vulnerability.

Reviewed by eLife

Enteric glia regulate Paneth cell secretion and intestinal microbial ecology

1. Aleksandra Prochera
2. Anoohya N Muppirala
3. Gavin A Kuziel
4. Salima Soualhi
5. Amy Shepherd
6. Liang Sun
7. Biju Issac
8. Harry J Rosenberg
9. Farah Karim
10. Kristina Perez
11. Kyle H Smith
12. Tonora H Archibald
13. Seth Rakoff-Nahoum
14. Susan J Hagen
15. Meenakshi Rao

• Curated by eLife

  eLife Assessment

  This study presents important findings on the function of enteric glia expressing proteolipid protein 1 (PLP1+ glia). The evidence supporting the claims of the authors is solid, although the inclusion of additional data showing the mechanisms by which PLP1+ enteric glia acts on Paneth cells would have strengthened the study. The work will be of interest to colleagues studying intestinal biology.

Reviewed by eLife

Increased reluctant vesicles underlie synaptic depression by GPR55 in axon terminals of cerebellar Purkinje cells

1. Takuma Inoshita
2. Shin-ya Kawaguchi

• Curated by eLife

  eLife Assessment

  This is an important study reporting that activation of the presynaptic GPR55 receptor suppresses synaptic transmission by modulating GABA release through the reduction of the readily releasable pool without affecting the presynaptic AP waveform and calcium influx. The evidence supporting this claim is compelling and based on an impressive array of techniques including patch-clamp recordings from the axon terminals of cerebellar Purkinje cells and fluorescent imaging of vesicular exocytosis. However, a few technical issues leave some questions open, these include uncertainty regarding the specificity of pharmacological agents and the nature of the endogenous process that would activate this pathway in vivo. In the current form, the evidence indicating that synaptic vesicles become insensitive to VGCC activation in the presence of GPR55 is weak and would need to be supported with additional experimental data.

Reviewed by eLife

Combined transcriptomic, connectivity, and activity profiling of the medial amygdala using highly amplified multiplexed in situ hybridization (hamFISH)

1. Mathew D Edwards
2. Ziwei Yin
3. Risa Sueda
4. Alina Gubanova
5. Chang S Xu
6. Virág Lakner
7. Megan Murchie
8. Chi-Yu Lee
9. Kristal Ng
10. Karolina Farrell
11. Rupert Faraway
12. Subham Ganguly
13. Elina Jacobs
14. Bogdan Bintu
15. Yoh Isogai

• Curated by eLife

  eLife Assessment

  Overall, this is an important work: the new methodology of hamFISH is a key additional tool for the assessment of the expression of multiple genes simultaneously. The authors provide convincing evidence of the utility of this approach on Medial Amygdala (MeA) tissue leveraging previous a transcriptomic dataset for gene selection. The authors also present a deeper dive into putative relationships between the on-tissue expression of subsets of genes and connectivity and behavioral regulation. The putative biological insights are intriguing, although preliminary, but notably they set up questions for future studies.

Reviewed by eLife

Visually-guided compensation of deafening-induced song deterioration

1. Manon Rolland
2. Anja T. Zai
3. Richard H. R. Hahnloser
4. Catherine Del Negro
5. Nicolas Giret

Reviewed by preLights

Modeling and Simulation of Neocortical Micro- and Mesocircuitry. Part II: Physiology and Experimentation

1. James B Isbister
2. András Ecker
3. Christoph Pokorny
4. Sirio Bolaños-Puchet
5. Daniela Egas Santander
6. Alexis Arnaudon
7. Omar Awile
8. Natali Barros-Zulaica
9. Jorge Blanco Alonso
10. Elvis Boci
11. Giuseppe Chindemi
12. Jean-Denis Courcol
13. Tanguy Damart
14. Thomas Delemontex
15. Alexander Dietz
16. Gianluca Ficarelli
17. Mike Gevaert
18. Joni Herttuainen
19. Genrich Ivaska
20. Weina Ji
21. Daniel Keller
22. James King
23. Pramod Kumbhar
24. Samuel Lapere
25. Polina Litvak
26. Darshan Mandge
27. Eilif B Muller
28. Fernando Pereira
29. Judit Planas
30. Rajnish Ranjan
31. Maria Reva
32. Armando Romani
33. Christian Rössert
34. Felix Schürmann
35. Vishal Sood
36. Aleksandra Teska
37. Anil Tuncel
38. Werner Van Geit
39. Matthias Wolf
40. Henry Markram
41. Srikanth Ramaswamy
42. Michael W Reimann

• Curated by eLife

  eLife Assessment

  This study reports a model of 8 somatosensory areas of the rat cortex consisting of 4.2 million morphologically and electrically detailed neurons. The authors carry out simulation experiments aimed at understanding how multiscale organization of the cortical network shapes neural activity. While the reviewers found the results to be solid, they note that they could have likely been obtained using a much smaller portion of the model. Nonetheless, the release of the modeling platform represents a significant contribution to the field by providing a valuable resource for the scientific community.

Reviewed by eLife

Robust variability of grid cell properties within individual grid modules enhances encoding of local space

1. William T Redman
2. Santiago Acosta-Mendoza
3. Xue-Xin Wei
4. Michael J Goard

• Curated by eLife

  eLife Assessment

  This valuable study examines the variability in spacing and direction of entorhinal grid cells, providing convincing evidence that such variability helps disambiguate locations within an environment. This study will be of interest to neuroscientists working on spatial navigation and, more broadly, on neural coding.

Reviewed by eLife