1. Mapping Serotonergic Dynamics using Drug-Modulated Molecular Connectivity

    This article has 7 authors:
    1. TM Ionescu
    2. M Amend
    3. R Hafiz
    4. A Maurer
    5. BB Biswal
    6. HF Wehrl
    7. K Herfert
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important paper on measuring molecular connectivity using combined serotonin PET and resting-state fMRI provides both novel methods for studying the brain as well as insights into the effects of ecstasy administration. The methods are solid, with a few doubts that need to be dispelled surrounding the high anaesthetic dose used.

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    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  2. Electrophysiological dynamics of a triple network model of cognitive control: A multi-experiment replication

    This article has 2 authors:
    1. Anup Das
    2. Vinod Menon
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this manuscript, the authors present valuable findings on the apparent role of a salience-network anterior insula node in directing fronto-parietal and default-mode network activity within a tripartite network during control of memory, drawn from an impressive invasive human neurophysiological dataset. While we commend the use of a large intracranial EEG dataset to approach this question, the study at present is incomplete in its methodologies, analysis, and interpretation to support the authors' central claims. The manuscript could be improved by addressing the concerns described.

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    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  3. Distinct release properties of glutamate/GABA co-transmission serve as a frequency-dependent filtering of supramammillary inputs

    This article has 6 authors:
    1. Himawari Hirai
    2. Kohtarou Konno
    3. Miwako Yamasaki
    4. Masahiko Watanabe
    5. Takeshi Sakaba
    6. Yuki Hashimotodani
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important work provides evidence that glutamate and GABA are released from different synaptic vesicles at supramammillary axon terminals onto granule cells of the dentate gyrus. The study uses complementary electrophysiological and anatomical experimental approaches. Together, these provide solid evidence that the co-release of glutamate and GABA from different vesicles within the same terminal could modulate granule cell firing in a frequency-dependent manner, although thorough elimination of alternative mechanisms would have strengthened the study. The work will be of interest to neuroscientists investigating co-release of neurotransmitters in various synapses in the brain and those interested in subcortical control of hippocampal function.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  4. Remote automated delivery of mechanical stimuli coupled to brain recordings in behaving mice

    This article has 17 authors:
    1. Justin Burdge
    2. Anissa Jhumka
    3. Simon Ogundare
    4. Nicholas Baer
    5. Sasha Fulton
    6. Brittany Bistis
    7. William Foster
    8. Andre Toussaint
    9. Miao Li
    10. Yosuke M. Morizawa
    11. Leah Yadessa
    12. Ashar Khan
    13. Abednego Delinois
    14. Wadzanayi Mayiseni
    15. Noah Loran
    16. Guang Yang
    17. Ishmail Abdus-Saboor
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study describes the development and validation of an Automated Reproducible Mechano-stimulator (ARM), a valuable tool for standardizing and automating somatosensory behavior experiments. The data supporting the use of the ARM system are compelling, though the determination of whether that device emits any sounds, including in the ultrasonic range when in operation or when at rest, would add value to the study. Nevertheless, the ARM system is anticipated to be popular amongst somatosensory and pain researchers.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  5. Probing PAC1 receptor activation across species with an engineered sensor

    This article has 14 authors:
    1. Reto B Cola
    2. Salome N Niethammer
    3. Preethi Rajamannar
    4. Andrea Gresch
    5. Musadiq A Bhat
    6. Kevin Assoumou
    7. Elyse Williams
    8. Patrick Hauck
    9. Nina Hartrampf
    10. Dietmar Benke
    11. Miriam Stoeber
    12. Gil Levkowitz
    13. Sarah Melzer
    14. Tommaso Patriarchi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental paper reports a new biosensor to study G protein-coupled receptor activation by the pituitary adenylyl cyclase-activating polypeptide (PACAP) in cell culture, ex vivo (mouse brain slices), and in vivo (zebrafish, mouse). Convincing data are presented that show the new sensor works with high affinity in vitro, while requiring very high (non-physiological) concentrations of exogenous PACAP when applied to intact tissues. The sensor has not yet been used to detect endogenously released PACAP, raising questions about whether the sensor can be used for its intended purpose. While further work must be pursued to achieve broad in vivo applications under physiological conditions, the new tool will be of interest to cell biologists, especially those studying the large and significant GPCR family.

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    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  6. ATUM-Tomo: A multi-scale approach to cellular ultrastructure by combined volume scanning electron microscopy and electron tomography

    This article has 12 authors:
    1. Georg Kislinger
    2. Gunar Fabig
    3. Antonia Wehn
    4. Lucia Rodriguez
    5. Hanyi Jiang
    6. Cornelia Niemann
    7. Andrey S Klymchenko
    8. Nikolaus Plesnila
    9. Thomas Misgeld
    10. Thomas Müller-Reichert
    11. Igor Khalin
    12. Martina Schifferer
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The present paper describes an important methodological development that combines light (confocal) microscopy with scanning and transmission EM and EM tomography. The method expands the level of structural detail accessible to large-volume EM studies and thus represents an approach to integrate analyses of cellular and sub-cellular structures in biological samples. The study, which provides a compelling proof-of-principle, will be of particular value to cell biologists interested in the in-depth interpretation of high-resolution ultrastructural information from sparsely distributed targets - at multiple scales and in diverse biological structures.

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    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
  7. Sex-peptide targets distinct higher order processing neurons in the brain to induce the female post-mating response

    This article has 4 authors:
    1. Mohanakarthik P. Nallasivan
    2. Deepanshu N.D. Singh
    3. Mohammed Syahir R. S. Saleh
    4. Matthias Soller
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study provides new insights into the neural circuits involved in post-mating responses in Drosophila females. It presents convincing evidence that the circuits for mating receptivity and egg-laying are distinct. A more thorough discussion regarding the integration of the new findings into the current understanding of post-mating behavior as well as clarification of some experimental details would further improve the manuscript.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  8. Systematic annotation of a complete adult male Drosophila nerve cord connectome reveals principles of functional organisation

    This article has 23 authors:
    1. Elizabeth C Marin
    2. Billy J Morris
    3. Tomke Stürner
    4. Andrew S Champion
    5. Dominik Krzeminski
    6. Griffin Badalamente
    7. Marina Gkantia
    8. Christopher R Dunne
    9. Katharina Eichler
    10. Shin-ya Takemura
    11. Imaan FM Tamimi
    12. Siqi Fang
    13. Sung Soo Moon
    14. Han SJ Cheong
    15. Feng Li
    16. Philipp Schlegel
    17. Sebastian E Ahnert
    18. Stuart Berg
    19. Janelia FlyEM Project Team
    20. Gwyneth M Card
    21. Marta Costa
    22. David Shepherd
    23. Gregory SXE Jefferis
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental study provides a near-comprehensive anatomical description and annotation of neurons in a male Drosophila ventral nerve cord, based on large-scale circuit reconstruction from electron microscopy. This connectome resource will be of substantial interest to neuroscientists interested in sensorimotor control, neural development, and analysis of brain connectivity. However, although the evidence is extensive and compelling, the presentation of results in this very large manuscript lacks clarity and concision.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  9. Human adherent cortical organoids in a multiwell format

    This article has 6 authors:
    1. Mark van der Kroeg
    2. Sakshi Bansal
    3. Maurits Unkel
    4. Hilde Smeenk
    5. Steven A Kushner
    6. Femke MS de Vrij
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This paper describes an important advance in a 2D in vitro neural culture system to generate mature, functional, diverse, and geometrically consistent cultures, in a 384-well format with defined dimensions and the absence of the necrotic core, which persists for up to 300 days. The well-based format and conserved geometry make it a promising tool for arrayed screening studies. Some of the evidence is incomplete and could benefit from a more direct head-to-head comparison with more standard culture methods and standardization of cell seeding density as well as further data on reproducibility in each well and for each cell line.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  10. Revealing unexpected complex encoding but simple decoding mechanisms in motor cortex via separating behaviorally relevant neural signals

    This article has 4 authors:
    1. Yangang Li
    2. Xinyun Zhu
    3. Yu Qi
    4. Yueming Wang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a useful method for the extraction of behaviour-related activity from neural population recordings based on a specific deep learning architecture, a variational autoencoder. Although the authors performed thorough benchmarking of their method in the context of decoding behavioural variables, the evidence supporting claims about encoding is incomplete as the results may stem, in part, from the properties of the method itself.

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    This article has 18 evaluationsAppears in 1 listLatest version Latest activity