Molecular Biology

A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plants

1. Mingming Chen
2. Naoyoshi Kumakura
3. Hironori Saito
4. Ryan Muller
5. Madoka Nishimoto
6. Mari Mito
7. Pamela Gan
8. Nicholas T Ingolia
9. Ken Shirasu
10. Takuhiro Ito
11. Yuichi Shichino
12. Shintaro Iwasaki

• Curated by eLife

  Evaluation Summary:

  In this important paper, Chen and colleagues identify a species of fungus, Ophiocordyceps sp. BRM1, that is able to grow on Aglaia sp. plants despite their production of rocaglate inhibitors of the eIF4A translation initiation factor. Through a series of solid experiments, the authors identify an amino acid substitution encoded in the fungal eIF4A gene that preserves eIF4A activity in the presence of these compounds. The authors conclude the substitution evolved to bypass this defense mechanism, similar to the way in which the plant itself bypasses it. The work will be of interest to fungal biologists and colleagues studying plant-microbe interactions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

A novel SATB1 protein isoform with different biophysical properties

1. Tomas Zelenka
2. Dionysios-Alexandros Papamatheakis
3. Petros Tzerpos
4. Giorgos Panagopoulos
5. Konstantinos C. Tsolis
6. Vassilis M. Papadakis
7. Dimitris Mariatos Metaxas
8. George Papadogkonas
9. Eleftherios Mores
10. Manouela Kapsetaki
11. Joseph Papamatheakis
12. David Stanek
13. Charalampos Spilianakis

Reviewed by Review Commons

Highly efficient generation of isogenic pluripotent stem cell models using prime editing

1. Hanqin Li
2. Oriol Busquets
3. Yogendra Verma
4. Khaja Mohieddin Syed
5. Nitzan Kutnowski
6. Gabriella R Pangilinan
7. Luke A Gilbert
8. Helen S Bateup
9. Donald C Rio
10. Dirk Hockemeyer
11. Frank Soldner

• Curated by eLife

  Evaluation Summary:

  In this manuscript, Li et al directly compare different editing strategies for human pluripotent stem cells. They demonstrate that prime editing is more efficient and precise, compared with double-strand break-based methods. They also confirm the suitability of prime editing for the introduction of different mutations related to Parkinson's disease as a model.

  (This preprint has been reviewed by eLife. We include the joint public review from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

RNA localization mechanisms transcend cell morphology

1. Raeann Goering
2. Ankita Arora
3. Megan C Pockalny
4. J Matthew Taliaferro

• Curated by eLife

  Evaluation Summary:

  Goering and colleagues investigate subcellular RNA localization across different cell types and species. The major insight is that there may be general mechanisms and specifically conserved proteins that regulate RNA localization in diverse cell types and morphologies. This manuscript will be of interest to those studying gene expression and how its regulation occurs within the cell.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Validation of scRNA-seq by scRT-ddPCR using the example of ErbB2 in MCF7 cells

1. Tobias Lange
2. Tobias Groß
3. Ábris Jeney
4. Julia Scherzinger
5. Elly Sinkala
6. Christoph Niemöller
7. Stefan Zimmermann
8. Peter Koltay
9. Felix von Stetten
10. Roland Zengerle
11. Csaba Jeney

Reviewed by Review Commons

Disruption of Smarce1, a component of the SWI/SNF chromatin remodeling complex, decreases nucleosome stability in mouse embryonic stem cells and impairs differentiation

1. Katsunobu Kashiwagi
2. Junko Yoshida
3. Hiroshi Kimura
4. Kyoji Horie

Reviewed by Review Commons

ATP induced conformational change of axonemal outer dynein arms studied by cryo-electron tomography

1. Noemi Zimmermann
2. Akira Noga
3. Jagan Mohan Obbineni
4. Takashi Ishikawa

Reviewed by Review Commons

The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth

1. Marina Badenes
2. Emma Burbridge
3. Ioanna Oikonomidi
4. Abdulbasit Amin
5. Érika de Carvalho
6. Lindsay Kosack
7. Camila Mariano
8. Pedro Domingos
9. Pedro Faísca
10. Colin Adrain

Reviewed by Review Commons

The establishment of variant surface glycoprotein monoallelic expression revealed by single-cell RNA-seq of Trypanosoma brucei in the tsetse fly salivary glands

1. Sebastian Hutchinson
2. Sophie Foulon
3. Aline Crouzols
4. Roberta Menafra
5. Brice Rotureau
6. Andrew D. Griffiths
7. Philippe Bastin

• Curated by eLife

  Evaluation Summary:

  All reviewers think the study will be really valuable for the field, especially after re-writing to include a detailed comparison with results that were previously published. We all appreciate the clear identification of a gamete sub-population, and also thought that the discovery of low activation of all VSG expression sites was intriguing and will be of considerable interest to those in the field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers all agreed to share their names with the authors.)

Reviewed by eLife

Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure

1. Ana Novačić
2. Dario Menéndez
3. Jurica Ljubas
4. Slobodan Barbarić
5. Françoise Stutz
6. Julien Soudet
7. Igor Stuparević

Reviewed by Review Commons