Microbiology

Three metabolic pathways replenishing the one-carbon pool collectively support growth and virulence of Listeria monocytogenes

1. Sandra Freier
2. Sarah Frentzel
3. Moritz Müller
4. Susan Scheffler
5. Sabrina Wamp
6. Tim Engelgeh
7. Janina Döhling
8. Dunja Bruder
9. Sascha Kahlfuss
10. Sven Halbedel

• Curated by eLife

  eLife Assessment

  One-carbon tetrahydrofolate metabolism plays a crucial role in producing essential metabolic intermediates. In this valuable study, the authors employ a solid genetics-based approach to demonstrate that three distinct metabolic pathways are essential for synthesising 1C-tetrahydrofolates (1C-THF). Disrupting any of these pathways impairs both growth and virulence.

Reviewed by eLife

Unexpected plasticity in the life cycle of Trypanosoma brucei

1. Carina Praisler
2. Jaime N Lisack
3. Anna Sophie Kreis
4. Laura Hauf
5. Johanna Krenzer
6. Fabian Imdahl
7. Markus Engstler

• Curated by eLife

  eLife Assessment

  The manuscript concerns a fundamental and controversial question in Trypanosoma brucei biology and the parasite life cycle, whether or not dividing slender bloodstream forms must transition to growth-arrested stumpy forms before differentiating to the procyclic form in the Tsetse midgut. The authors provide further evidence that slender bloodstream forms can infect Tsetse flies, and that although their differentiation is considerably delayed, they do not become classical stumpy forms during the process. The study is solid in design and execution, and addresses several criticisms made of the authors' earlier work, although discrepancies with results from other laboratories remain.

Reviewed by eLife

Thermoregulation network governing virulence of a critical human fungal pathogen

1. Vikas Yadav
2. Joseph Heitman

Reviewed by Review Commons

A mitochondrial quality control mechanism reverses the phagosome maturation arrest caused by Mycobacterium tuberculosis

1. Surbhi Verma
2. Shweta Thakur
3. Mrinmoy Das
4. Raman D Sharma
5. Vikas Yadav
6. Priya Sharma
7. Mardiana Marzuki
8. Shihui Foo
9. Giulia M Piperno
10. Mehak Z Khan
11. Babu Mathew
12. Meenu Bajpai
13. Jaswinder Singh Maras
14. Shanshan Howland
15. Sovan Sarkar
16. Federica Benvenuti
17. Amit Singh
18. Vinay Nandicoori
19. Amit Singhal
20. Dhiraj Kumar

• Curated by eLife

  eLife Assessment

  Verma and colleagues interrogate the mechanisms of phagosome maturation arrest during Mycobacterium tuberculosis infection. While cellular events that culminate in this arrest have been largely elucidated, involvement of other organelles, such as mitochondria, has not been highlighted mechanistically. In this valuable study, elements of mitochondrial quality control, such as mitophagy and mitochondrial-derived vesicles involvement, are shown to be paramount in the host-pathogen tussle. The evidence supporting the main conclusions is solid, based on multiple complementary approaches and appropriate controls, although some central mechanistic aspects of the proposed pathway remain only partially resolved.

Reviewed by eLife

The vaccine candidate Liver Stage Antigen 3 is exported during Plasmodium falciparum infection and required for liver-stage development

1. Robyn McConville
2. Ryan WJ Steel
3. Matthew T O’Neill
4. Alan F Cowman
5. Norman Kneteman
6. Justin A Boddey

• Curated by eLife

  eLife Assessment

  This useful study provides new insights into the liver-stage antigen LSA3, its export to erythrocytes, and its role in liver-stage development. While the functional importance of LSA3 is well demonstrated, the data underlying the conclusions regarding antibody specificity, liver-stage localization, and phenotype remain incomplete. A key strength of the study is the use of mosquito and humanized mouse models to access life-cycle stages that are rarely studied in most laboratories.

Reviewed by eLife