Microbiology

Lysosomal activation in bladder epithelium enhances intracellular antibiotic clearance of uropathogenic Escherichia coli

1. Kathrin Tomasek
2. Kristina Skurvydaite
3. Gauri Paduthol
4. Allison M. Burns
5. Léa Schlunke
6. Valentin Borgeat
7. Christian Pasquali
8. Mario Romani
9. John D. McKinney

Reviewed by Review Commons

Identifying and targeting the Mg-Fe-PmrAB regulatory circuit reverses phosphate starvation-driven polymyxin resistance in Enterobacteriaceae

1. Guangming Zhang
2. Ziqing Deng
3. Jiezhang Jiang
4. Minji Wang
5. Xiaoyuan Wang
6. Xiaoyun Liu
7. Aixin Yan

• Curated by eLife

  eLife Assessment

  This study characterizes a potentially targetable mechanism by which phosphate scarcity drives polymyxin B resistance in Enterobacteriaceae. The findings are important. While some aspects of the approach are very strong, particularly the diversity of techniques, it is recommended to include genetic controls and antibiotic resistance experiments in order to strengthen the evidence, which is currently solid. The clarity and presentation of the findings could also be improved.

Reviewed by eLife

A dual role for PGLYRP1 in host defense and immune regulation during B. pertussis infection

1. David M Rickert
2. Sasha Cardozo
3. Nicholas H Carbonetti
4. William E Goldman
5. Karen M Scanlon
6. Ciaran Skerry

• Curated by eLife

  eLife Assessment

  Rickert and colleagues demonstrate that the host peptidoglycan-binding protein PGLYRP1 has both beneficial and detrimental effects on Bordetella pertussis infection in mice. Using a solid array of techniques, the study provides useful insights into how the peptidoglycan fragment tracheal cytotoxin alters host immune responses, dampening inflammatory responses later in B. pertussis infection. These studies indicate that release of peptidoglycan fragments with particular structures can be used by bacteria to modulate NOD1 versus NOD2 responses to their advantage.

Reviewed by eLife

Niche exclusion of a lung pathogen in mice with designed probiotic communities

1. Kelsey E Hern
2. Ashlee M Phillips
3. Catherine M Mageeney
4. Kelly P Williams
5. Anupama Sinha
6. Hans K Carlson
7. Kunal Poorey
8. Nicole M Collette
9. Steven S Branda
10. Adam P Arkin

• Curated by eLife

  eLife Assessment

  This study presents a valuable framework for the rational design of bacterial probiotics to protect against respiratory infections. The evidence supporting the central claim - that metabolic niche overlap predicts probiotic efficacy - is solid, combining innovative in vitro modeling with in vivo validation, though the model appears less effective for probiotics that rely on antimicrobial metabolite production.

Reviewed by eLife

Simple Sequence Repeats Mediate Phase Variation of the Mucoid Phenotype in Hypervirulent Klebsiella pneumoniae

1. Oliwia Stawarska
2. Aarabhi Ravikrishnan Nair
3. Ania W. Y. Man
4. Luke R. Green
5. Ozcan Gazioglu
6. Francesco Flandi
7. Margaret M. C. Lam
8. Kathryn E. Holt
9. Hasan Yesilkaya
10. Marco R. Oggioni
11. Jose A. Bengoechea
12. Charlotte Odendall
13. Alex J. McCarthy
14. Joseph J. Wanford

Reviewed by PREreview

Mycobacterium tuberculosis partitions the Krebs cycle under iron starvation

1. Agnese Serafini
2. Acely Garza-Garcia
3. Davide Sorze
4. Luiz Pedro Sorio de Carvalho
5. Riccardo Manganelli

• Curated by eLife

  eLife Assessment

  This well-designed, valuable study uses isotope tracing to analyse how iron limitation alters TCA cycle metabolism in Mycobacterium tuberculosis, revealing potential antibiotic targets for non-replicating bacteria in the host. The evidence is solid, providing insights into metabolic remodelling under iron-limited conditions.

Reviewed by eLife

Membrane binding controls the ATPase cycle and localization of MinD in Bacillus subtilis

1. Helge Feddersen
2. Charlotte Dyckmans
3. Marc Bramkamp

• Curated by eLife

  eLife Assessment

  This important study provides convincing data suggesting that subcellular localization of the spatial regulator of cell division, MinD, is an intrinsic feature of the protein's ability to associate with the membrane as both a dimer and a monomer. These findings distinguish the behavior of MinD in B. subtilis from its counterpart in E. coli and suggest that there is not a need to invoke additional localization factors. The reviewers felt that the revisions, particularly the additional experiments and changes to the text to make the experimental design and conclusions clearer, improve the quality of the manuscript.

Reviewed by eLife

A synthetic peptide mimic kills Candida albicans and synergistically prevents infection

1. Sebastian Schaefer
2. Raghav Vij
3. Jakob L. Sprague
4. Sophie Austermeier
5. Hue Dinh
6. Peter R. Judzewitsch
7. Sven Müller-Loennies
8. Taynara Lopes Silva
9. Eric Seemann
10. Britta Qualmann
11. Christian Hertweck
12. Kirstin Scherlach
13. Thomas Gutsmann
14. Amy K. Cain
15. Nathaniel Corrigan
16. Mark S. Gresnigt
17. Cyrille Boyer
18. Megan D. Lenardon
19. Sascha Brunke

Reviewed by Rapid Reviews Infectious Diseases

A fast-acting inhibitor of blood-stage P. falciparum with mechanism distinct from artemisinin and chloroquine

1. Stephanie Kabeche
2. Thomas Meister
3. Ellen Yeh

Reviewed by Rapid Reviews Infectious Diseases

Functional genomics screens reveal a role for TBC1D24 and SV2B in antibody-dependent enhancement of dengue virus infection

1. Laura Belmont
2. Maya Contreras
3. Catiana H. Cartwright-Acar
4. Caleb D. Marceau
5. Aditi Agrawal
6. Lisa M. Levoir
7. Jay Lubow
8. Leslie Goo

Reviewed by Rapid Reviews Infectious Diseases